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Gene information
General information
TC007882 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 2134232..2147716
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007882
    iBeetle screen iB_01278
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007882 RA
      MAVCLVGLV LLALALDAG GFYISQKSA TKVVNNETF 
      IVFCRDDVG GLPIKWRNQ KGEILGPKT RPAVHNTSY 
      GTSIMFTNP LRVEDSGNY TCSTGKEER VFQFLVDAP 
      LKFTDTSTN QTAVEGSEF NLKCEVKGG STSWSMDEG 
      SAITDNAKY AVLGDGLLI RNVTRSDSK TYICKGVQP 
      STGTVLDRP IHLHVLHKP VHPNHPQRH VRQEVIYGY 
      INGTANLTC VTIANPPAT FVWKKHRDP KKKIGKTIS 
      ESDEISILQ LHIRDKSFF DNYTCIARN KYGVYEEHF 
      TLKEGARPL PPSQLQLEE IKSKSLIFK IHGPANSSE 
      MDLGTKGFN VKYKKIDDN EWKQKDFNI TQDNLYSLE 
      GLEPNTEYE VQAATRNAA GLSVYVSSS TYTTSRGAA 
      VHSTLPHVL GAAIISLKF FW
    CDS sequence
    • >TC007882 RA
      atggcagtg tgtttagtc gggttggtc ctgctggca 
      ctggcgtta gatgccggc ggattttat atatctcag 
      aagtcggcg acgaaggta gtaaacaac gaaacgttt 
      atagttttt tgtcgtgac gacgtcgga ggccttccg 
      ataaaatgg agaaaccaa aaaggcgag attttgggt 
      ccgaagaca cgtccagct gtccacaat acgtcttac 
      ggcacttcg atcatgttc actaatccc ctgagggtg 
      gaggattcg ggcaattat acgtgttcg acgggcaag 
      gaagagcga gtgttccag tttttggtg gatgctccg 
      ctcaaattc accgatact tcgacgaac caaacggcc 
      gtagaaggc tccgaattt aacttaaaa tgcgaggtc 
      aaaggcggc tcgacctcc tggtcaatg gacgaaggc 
      tcggcaatt acagataat gcaaagtac gctgttctt 
      ggtgacggc ctcctgata cgaaacgtc acccgatct 
      gatagcaaa acctacatc tgtaaagga gttcagcca 
      agcacagga acggtcctc gaccgcccc atccacctc 
      cacgtgttg cacaagccg gtccacccg aaccatccg 
      cagagacac gtccgacaa gaagttatc tacggctac 
      atcaacggc accgccaat ctgacttgt gtcacgatt 
      gctaatccc ccggccacc ttcgtctgg aaaaaacac 
      agagacccc aagaagaaa atcgggaaa accatcagc 
      gaatcggac gaaatctcc atcctgcaa ttgcacatt 
      cgcgacaag tccttcttc gacaactac acgtgcatt 
      gcccggaat aagtacggg gtgtacgag gagcatttc 
      accctgaaa gagggcgcc cgaccactg ccgcccagc 
      cagctccag ttggaggaa ataaaaagc aagtcgctc 
      atattcaaa atacacggc ccggcgaat tcgtccgag 
      atggatttg ggaacgaaa ggatttaac gtgaagtat 
      aagaagatt gatgataac gaatggaaa caaaaggac 
      tttaatatc acacaagac aatttgtat tccttggaa 
      ggtttggag ccaaacacc gaatacgaa gttcaagcg 
      gcgaccagg aacgctgca ggtttgagt gtttacgtc 
      tcctccagc acttacacg acttcgcga ggcgctgct 
      gtccactcg actttacca catgttctc ggcgccgca 
      attatatca ctgaaattc ttttggtga
    mRNA sequence
    • >TC007882 RA
      tcgatgtaa aatagtcgg agtttctag tcttccttg 
      tacggtcca tgtggcgcc agtacaatt ttgcgcgcg 
      cgacgatca tctcgtcct ggcagcctt taattcgaa 
      gcccatatt gcacctcgt tgtagaaat tgtggctga 
      aagacgtgg ctaagtacg tgaagtttt aactgccat 
      tgatggatt taacgctct tcatgtgac gatctaaac 
      atggcagtg tgtttagtc gggttggtc ctgctggca 
      ctggcgtta gatgccggc ggattttat atatctcag 
      aagtcggcg acgaaggta gtaaacaac gaaacgttt 
      atagttttt tgtcgtgac gacgtcgga ggccttccg 
      ataaaatgg agaaaccaa aaaggcgag attttgggt 
      ccgaagaca cgtccagct gtccacaat acgtcttac 
      ggcacttcg atcatgttc actaatccc ctgagggtg 
      gaggattcg ggcaattat acgtgttcg acgggcaag 
      gaagagcga gtgttccag tttttggtg gatgctccg 
      ctcaaattc accgatact tcgacgaac caaacggcc 
      gtagaaggc tccgaattt aacttaaaa tgcgaggtc 
      aaaggcggc tcgacctcc tggtcaatg gacgaaggc 
      tcggcaatt acagataat gcaaagtac gctgttctt 
      ggtgacggc ctcctgata cgaaacgtc acccgatct 
      gatagcaaa acctacatc tgtaaagga gttcagcca 
      agcacagga acggtcctc gaccgcccc atccacctc 
      cacgtgttg cacaagccg gtccacccg aaccatccg 
      cagagacac gtccgacaa gaagttatc tacggctac 
      atcaacggc accgccaat ctgacttgt gtcacgatt 
      gctaatccc ccggccacc ttcgtctgg aaaaaacac 
      agagacccc aagaagaaa atcgggaaa accatcagc 
      gaatcggac gaaatctcc atcctgcaa ttgcacatt 
      cgcgacaag tccttcttc gacaactac acgtgcatt 
      gcccggaat aagtacggg gtgtacgag gagcatttc 
      accctgaaa gagggcgcc cgaccactg ccgcccagc 
      cagctccag ttggaggaa ataaaaagc aagtcgctc 
      atattcaaa atacacggc ccggcgaat tcgtccgag 
      atggatttg ggaacgaaa ggatttaac gtgaagtat 
      aagaagatt gatgataac gaatggaaa caaaaggac 
      tttaatatc acacaagac aatttgtat tccttggaa 
      ggtttggag ccaaacacc gaatacgaa gttcaagcg 
      gcgaccagg aacgctgca ggtttgagt gtttacgtc 
      tcctccagc acttacacg acttcgcga ggcgctgct 
      gtccactcg actttacca catgttctc ggcgccgca 
      attatatca ctgaaattc ttttggtga ctgtaaacc 
      ggaaatata cttcattta ctatacgta tggctacca 
      tagggcgag accaggata ataagtcta ataaaacgt 
      tcctttcca caattcctc ttaatcttg tcatatcac 
      tcatcatct cacctcatt catttgtga tcgatcaaa 
      ttacaaagt gaggtcatg gccgaatga cctcatttt 
      gactacgaa tgtttattt ttaaaaagt gccattttg 
      tacatgtga tttagtcgg ttttgattg tttacgtag 
      accaaacta ttcagtgta aataataag tgtatgcta 
      attattgtt gactaacgt gccaatgtt aatgaatgt 
      tgcattatc ggtgtagct gtgttttta gtctcatgg 
      ctttgtttg gaatgactg acaataagg tcgatttgt 
      aacatatgt gagaagaca aaacgtttc aatagcatt 
      acaaatcct ataggcgac tgtttatca gaatttatt 
      cgacggtta tataacaaa ttgtaaaag tttatcgct 
      tttaattgt atttattac acagatccg tgatctaat 
      ttttgttgt ctagatata agacgtcgg ctcgcttcg 
      acagggctc taggaatag ttgttatcg ccctgccga 
      agcggccaa atccacgat caaataaat ttgtttttc 
      tataaatgt tgtttattt tcccttgac tccaaaaat 
      aacgaaaag tgagaacat ttcatatca caaagtact 
      ttagtctgt gacacttga aaaaagcta taactagtt 
      gagggaaag atattggat catgacaat ttttttcct 
      ccttggtaa aaaatagtg gaccgacca aattctaaa 
      ttgcttgca atatagttc tcgacaatc aataaaatt 
      tgtaataaa tagaatttc cgggcttga cacatttcc 
      aatagcagc aaaaatgaa tcgtgagct gtcagttcg 
      tcgaagata tcggttctt tccttggtc acctaaaaa 
      attctgaaa ggaataaat taatgcgag cttccacat 
      gaaagcaag cttctgcca tctacagcg ctccaacca 
      agtctcgga gcgaaacct gtacccaag taaattaaa 
      aacgtgata atagtgata acaatgaat cagcgacca 
      ggccaaggc cacaacctt tggcttatg gtgacccct 
      ggcacaatt ctttcccaa acattcggc tgcttgttt 
      attaaaggt catttgcat gtcaacaat aaaacgtcc 
      ttcccctat ttttagact gggaacgct gtctcagac 
      agtgctttc agcgaaaag cttatcatc gtccatggc 
      aaccgtcaa ctgtgacaa cttcatcga tttttgtgc 
      cgaaaattc accggcgat gaccagagg agcgtctcc 
      aagccggtt gggggtcct ctagctcta gaataagtc 
      tagagcatt cgatcagga tcgtcggta atattccgt 
      gcaccttac gcgcagact cctccacca acaaggaac 
      caaagaacc catcagcta aacgagtga cttcaccgg 
      ccggtagtt tggaaaaat tgagacact cgtgaaaat 
      tctcaatcg acgcaaaaa tgggcttgt ggagacgcc 
      tgccgaggg ttccttccg agagcaact ttgacagtg 
      agtactcct tttatctgt ggttggata acctacctc 
      aatattgat cttgacccc tttgcaaaa tttacggcc 
      actccagtt tcacaaaat tgcaaaacg gcgctctga 
      atccatgaa tgaaggctt cggcaatca tggtcctga 
      ccttgttcc aggaaaact cccgttgta attgtccat 
      tattactgt ttgctgaat tcgttaata attacttac 
      tcggcat