| General information | |||
| TC007869 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1977037..1979581 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007869 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01277 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC007869 RA
MWPKIIVLS ILLPFLNGQ LYFPDEKSG GYVQMQKEG PSEPILPTN LTELRGTTL YEQFVDKII STGILKLTV AMEKALTAA SPNNYESIV FAPVNIAGA LALVLLGSN GKTFDEIST IMGLATGVD IHNKSQLVH EQFGRLLEK LQITSGFDI GRQVNIANA VFVQNNYPI RQIYKNTAE MVYRSEVLN VDFETNPKS AEQIINAWV SERTKGKIT QILNETPDS STKVILASA MYFKAEWER PFFDGSTKR RPFYTNGRG AKSDVMVEM MSNGGLFPY YKDRDLNCE IIGFPYKGN QTTMYVVIP TNSDKTKLK ELEDKLDYK QVERLVANV KYTGAIVIF PKMRIDTTI DLKKSLKNL GVRSLFSPA EANLALLSP GKGAPGTNP VIGNSIQNP ISVNTTGND VIIFNRNGE VVNCTQIFN PKSNVSTCE QVVTDSSTP QKVVYKKFG NKVGRRIDG EITPRIAET LDNVRQYLN QQSNAERFE NPGLYADNV IHKVYMDIT ESGTEAAAS TSISLSRDG GRVTVRADV PFLFFIMHE ETKTMLFWG SVNTPTPNF K
- >TC007869 RA
atgtggcct aaaataatc gtcctatcc atactgcta ccgtttcta aatggccag ttgtacttc cctgacgaa aaaagcggt ggctacgtc cagatgcaa aaagaaggc ccatcggag ccaatttta ccgacaaac ctgactgaa ctaagagga accacactt tatgaacaa ttcgtagac aaaattatt tcaaccgga attctcaaa ctgactgta gcgatggaa aaagcccta actgcagca tcgcccaac aattacgaa agcattgtg tttgctccc gttaacatt gctggagca ctagccctt gtgcttttg ggctcaaat ggcaagact tttgacgag atttcgacc atcatgggg ctagcaact ggcgttgat attcacaat aagtcccaa ctagtccat gaacaattc gggagattg ttggaaaaa ctgcagatt acttcaggg tttgatatt gggcgtcaa gtcaacatt gcgaacgcc gtttttgtg caaaataat taccctatt cgacaaatt tacaaaaac acggctgaa atggtttac aggagcgag gttttgaac gtggatttt gaaacgaat ccaaaatcg gccgaacaa attataaac gcatgggtg tctgaacgg accaaaggc aaaatcacc caaattcta aacgaaact cccgacagc tcaaccaaa gtgattttg gccagtgct atgtacttc aaagccgag tgggagcga ccgtttttt gacgggagc acgaaaaga cgtcccttt tataccaac ggtcggggg gccaaaagt gacgttatg gtggaaatg atgtcaaac ggtggcttg ttcccttac tacaaagac cgggactta aactgcgaa ataatcggg tttccgtac aaaggcaac caaaccacc atgtacgtt gtaatcccc accaattcg gacaaaacg aaattaaaa gaactcgaa gacaagttg gactataag caagtcgag cgtttggtc gccaatgtg aaatacacg ggggctatc gtcatattt ccgaaaatg cgcatcgac acaacgatt gatttgaaa aaatcgctc aaaaattta ggcgtgcgt tcgctcttc agtccagct gaggcgaac ctagcgctg ctgtccccc ggaaaaggc gctccgggc acgaatccg gtcatcggc aactcgatc cagaaccca atttcggtc aacacgacc ggaaacgac gtcattata ttcaaccgg aacggagaa gtggtcaat tgtacgcaa attttcaac ccgaagagc aatgtcagt acttgcgag caggtcgtg accgattcc tcaactccg cagaaggtc gtgtacaag aagtttggg aataaagtt gggcggagg atcgacggg gagataact ccgcgaatt gccgaaact ttggataat gttagacag tatttaaat cagcagagc aacgcggag aggtttgaa aatccggga ctgtatgct gataatgtc attcataag gtgtatatg gatattact gagagtggg actgaagcg gccgcttcc acgtcgatt agtctgtcg agggatgga ggaagggtc acggttagg gccgatgtg ccctttttg tttttcatc atgcatgag gagaccaag accatgctg ttttggggg tcggtgaac acgcccaca cccaatttc aaataa
- >TC007869 RA
cagaggttg tgctgttgg tgctgtgtg gttgtaata ctgttttac caaaaattt acgtcttta gacaacttc tccttccga taacatgtg ctaatcttt tgtcgcaaa taagtgata aacaccata attaatttt aaacgtgag tcaagatgt ggcctaaaa taatcgtcc tatccatac tgctaccgt ttctaaatg gccagttgt acttccctg acgaaaaaa gcggtggct acgtccaga tgcaaaaag aaggcccat cggagccaa ttttaccga caaacctga ctgaactaa gaggaacca cactttatg aacaattcg tagacaaaa ttatttcaa ccggaattc tcaaactga ctgtagcga tggaaaaag ccctaactg cagcatcgc ccaacaatt acgaaagca ttgtgtttg ctcccgtta acattgctg gagcactag cccttgtgc ttttgggct caaatggca agacttttg acgagattt cgaccatca tggggctag caactggcg ttgatattc acaataagt cccaactag tccatgaac aattcggga gattgttgg aaaaactgc agattactt cagggtttg atattgggc gtcaagtca acattgcga acgccgttt ttgtgcaaa ataattacc ctattcgac aaatttaca aaaacacgg ctgaaatgg tttacagga gcgaggttt tgaacgtgg attttgaaa cgaatccaa aatcggccg aacaaatta taaacgcat gggtgtctg aacggacca aaggcaaaa tcacccaaa ttctaaacg aaactcccg acagctcaa ccaaagtga ttttggcca gtgctatgt acttcaaag ccgagtggg agcgaccgt tttttgacg ggagcacga aaagacgtc ccttttata ccaacggtc ggggggcca aaagtgacg ttatggtgg aaatgatgt caaacggtg gcttgttcc cttactaca aagaccggg acttaaact gcgaaataa tcgggtttc cgtacaaag gcaaccaaa ccaccatgt acgttgtaa tccccacca attcggaca aaacgaaat taaaagaac tcgaagaca agttggact ataagcaag tcgagcgtt tggtcgcca atgtgaaat acacggggg ctatcgtca tatttccga aaatgcgca tcgacacaa cgattgatt tgaaaaaat cgctcaaaa atttaggcg tgcgttcgc tcttcagtc cagctgagg cgaacctag cgctgctgt cccccggaa aaggcgctc cgggcacga atccggtca tcggcaact cgatccaga acccaattt cggtcaaca cgaccggaa acgacgtca ttatattca accggaacg gagaagtgg tcaattgta cgcaaattt tcaacccga agagcaatg tcagtactt gcgagcagg tcgtgaccg attcctcaa ctccgcaga aggtcgtgt acaagaagt ttgggaata aagttgggc ggaggatcg acggggaga taactccgc gaattgccg aaactttgg ataatgtta gacagtatt taaatcagc agagcaacg cggagaggt ttgaaaatc cgggactgt atgctgata atgtcattc ataaggtgt atatggata ttactgaga gtgggactg aagcggccg cttccacgt cgattagtc tgtcgaggg atggaggaa gggtcacgg ttagggccg atgtgccct ttttgtttt tcatcatgc atgaggaga ccaagacca tgctgtttt gggggtcgg tgaacacgc ccacaccca atttcaaat aatccggcg tttgtgagc actttataa acattattt acacgattg tgatttcgt tttttacct ttgtaccta tttattttt atgtgtaag tgtgcatta atattaaag atttgtcca ctct
