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Gene information
General information
TC007869 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 1977037..1979581
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007869
    iBeetle screen iB_01277
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007869 RA
      MWPKIIVLS ILLPFLNGQ LYFPDEKSG GYVQMQKEG 
      PSEPILPTN LTELRGTTL YEQFVDKII STGILKLTV 
      AMEKALTAA SPNNYESIV FAPVNIAGA LALVLLGSN 
      GKTFDEIST IMGLATGVD IHNKSQLVH EQFGRLLEK 
      LQITSGFDI GRQVNIANA VFVQNNYPI RQIYKNTAE 
      MVYRSEVLN VDFETNPKS AEQIINAWV SERTKGKIT 
      QILNETPDS STKVILASA MYFKAEWER PFFDGSTKR 
      RPFYTNGRG AKSDVMVEM MSNGGLFPY YKDRDLNCE 
      IIGFPYKGN QTTMYVVIP TNSDKTKLK ELEDKLDYK 
      QVERLVANV KYTGAIVIF PKMRIDTTI DLKKSLKNL 
      GVRSLFSPA EANLALLSP GKGAPGTNP VIGNSIQNP 
      ISVNTTGND VIIFNRNGE VVNCTQIFN PKSNVSTCE 
      QVVTDSSTP QKVVYKKFG NKVGRRIDG EITPRIAET 
      LDNVRQYLN QQSNAERFE NPGLYADNV IHKVYMDIT 
      ESGTEAAAS TSISLSRDG GRVTVRADV PFLFFIMHE 
      ETKTMLFWG SVNTPTPNF K
    CDS sequence
    • >TC007869 RA
      atgtggcct aaaataatc gtcctatcc atactgcta 
      ccgtttcta aatggccag ttgtacttc cctgacgaa 
      aaaagcggt ggctacgtc cagatgcaa aaagaaggc 
      ccatcggag ccaatttta ccgacaaac ctgactgaa 
      ctaagagga accacactt tatgaacaa ttcgtagac 
      aaaattatt tcaaccgga attctcaaa ctgactgta 
      gcgatggaa aaagcccta actgcagca tcgcccaac 
      aattacgaa agcattgtg tttgctccc gttaacatt 
      gctggagca ctagccctt gtgcttttg ggctcaaat 
      ggcaagact tttgacgag atttcgacc atcatgggg 
      ctagcaact ggcgttgat attcacaat aagtcccaa 
      ctagtccat gaacaattc gggagattg ttggaaaaa 
      ctgcagatt acttcaggg tttgatatt gggcgtcaa 
      gtcaacatt gcgaacgcc gtttttgtg caaaataat 
      taccctatt cgacaaatt tacaaaaac acggctgaa 
      atggtttac aggagcgag gttttgaac gtggatttt 
      gaaacgaat ccaaaatcg gccgaacaa attataaac 
      gcatgggtg tctgaacgg accaaaggc aaaatcacc 
      caaattcta aacgaaact cccgacagc tcaaccaaa 
      gtgattttg gccagtgct atgtacttc aaagccgag 
      tgggagcga ccgtttttt gacgggagc acgaaaaga 
      cgtcccttt tataccaac ggtcggggg gccaaaagt 
      gacgttatg gtggaaatg atgtcaaac ggtggcttg 
      ttcccttac tacaaagac cgggactta aactgcgaa 
      ataatcggg tttccgtac aaaggcaac caaaccacc 
      atgtacgtt gtaatcccc accaattcg gacaaaacg 
      aaattaaaa gaactcgaa gacaagttg gactataag 
      caagtcgag cgtttggtc gccaatgtg aaatacacg 
      ggggctatc gtcatattt ccgaaaatg cgcatcgac 
      acaacgatt gatttgaaa aaatcgctc aaaaattta 
      ggcgtgcgt tcgctcttc agtccagct gaggcgaac 
      ctagcgctg ctgtccccc ggaaaaggc gctccgggc 
      acgaatccg gtcatcggc aactcgatc cagaaccca 
      atttcggtc aacacgacc ggaaacgac gtcattata 
      ttcaaccgg aacggagaa gtggtcaat tgtacgcaa 
      attttcaac ccgaagagc aatgtcagt acttgcgag 
      caggtcgtg accgattcc tcaactccg cagaaggtc 
      gtgtacaag aagtttggg aataaagtt gggcggagg 
      atcgacggg gagataact ccgcgaatt gccgaaact 
      ttggataat gttagacag tatttaaat cagcagagc 
      aacgcggag aggtttgaa aatccggga ctgtatgct 
      gataatgtc attcataag gtgtatatg gatattact 
      gagagtggg actgaagcg gccgcttcc acgtcgatt 
      agtctgtcg agggatgga ggaagggtc acggttagg 
      gccgatgtg ccctttttg tttttcatc atgcatgag 
      gagaccaag accatgctg ttttggggg tcggtgaac 
      acgcccaca cccaatttc aaataa
    mRNA sequence
    • >TC007869 RA
      cagaggttg tgctgttgg tgctgtgtg gttgtaata 
      ctgttttac caaaaattt acgtcttta gacaacttc 
      tccttccga taacatgtg ctaatcttt tgtcgcaaa 
      taagtgata aacaccata attaatttt aaacgtgag 
      tcaagatgt ggcctaaaa taatcgtcc tatccatac 
      tgctaccgt ttctaaatg gccagttgt acttccctg 
      acgaaaaaa gcggtggct acgtccaga tgcaaaaag 
      aaggcccat cggagccaa ttttaccga caaacctga 
      ctgaactaa gaggaacca cactttatg aacaattcg 
      tagacaaaa ttatttcaa ccggaattc tcaaactga 
      ctgtagcga tggaaaaag ccctaactg cagcatcgc 
      ccaacaatt acgaaagca ttgtgtttg ctcccgtta 
      acattgctg gagcactag cccttgtgc ttttgggct 
      caaatggca agacttttg acgagattt cgaccatca 
      tggggctag caactggcg ttgatattc acaataagt 
      cccaactag tccatgaac aattcggga gattgttgg 
      aaaaactgc agattactt cagggtttg atattgggc 
      gtcaagtca acattgcga acgccgttt ttgtgcaaa 
      ataattacc ctattcgac aaatttaca aaaacacgg 
      ctgaaatgg tttacagga gcgaggttt tgaacgtgg 
      attttgaaa cgaatccaa aatcggccg aacaaatta 
      taaacgcat gggtgtctg aacggacca aaggcaaaa 
      tcacccaaa ttctaaacg aaactcccg acagctcaa 
      ccaaagtga ttttggcca gtgctatgt acttcaaag 
      ccgagtggg agcgaccgt tttttgacg ggagcacga 
      aaagacgtc ccttttata ccaacggtc ggggggcca 
      aaagtgacg ttatggtgg aaatgatgt caaacggtg 
      gcttgttcc cttactaca aagaccggg acttaaact 
      gcgaaataa tcgggtttc cgtacaaag gcaaccaaa 
      ccaccatgt acgttgtaa tccccacca attcggaca 
      aaacgaaat taaaagaac tcgaagaca agttggact 
      ataagcaag tcgagcgtt tggtcgcca atgtgaaat 
      acacggggg ctatcgtca tatttccga aaatgcgca 
      tcgacacaa cgattgatt tgaaaaaat cgctcaaaa 
      atttaggcg tgcgttcgc tcttcagtc cagctgagg 
      cgaacctag cgctgctgt cccccggaa aaggcgctc 
      cgggcacga atccggtca tcggcaact cgatccaga 
      acccaattt cggtcaaca cgaccggaa acgacgtca 
      ttatattca accggaacg gagaagtgg tcaattgta 
      cgcaaattt tcaacccga agagcaatg tcagtactt 
      gcgagcagg tcgtgaccg attcctcaa ctccgcaga 
      aggtcgtgt acaagaagt ttgggaata aagttgggc 
      ggaggatcg acggggaga taactccgc gaattgccg 
      aaactttgg ataatgtta gacagtatt taaatcagc 
      agagcaacg cggagaggt ttgaaaatc cgggactgt 
      atgctgata atgtcattc ataaggtgt atatggata 
      ttactgaga gtgggactg aagcggccg cttccacgt 
      cgattagtc tgtcgaggg atggaggaa gggtcacgg 
      ttagggccg atgtgccct ttttgtttt tcatcatgc 
      atgaggaga ccaagacca tgctgtttt gggggtcgg 
      tgaacacgc ccacaccca atttcaaat aatccggcg 
      tttgtgagc actttataa acattattt acacgattg 
      tgatttcgt tttttacct ttgtaccta tttattttt 
      atgtgtaag tgtgcatta atattaaag atttgtcca 
      ctct