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Gene information
General information
TC007868 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 1972509..1975665
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007868
    iBeetle screen iB_01276
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    tropharium potentially size increased - Number of animals showing the phenotype: 4
    tropharium posterior structure irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007868 RA
      MGDSLTECM EIFSQDGVQ LKTAQASFV NYLNLVGTL 
      YITEYANSN CRRCLEWKP NDVTVDSDV QDQEWAVVN 
      TVQKRSRTY SESLPPDST IKPKLLKVN FVDIKSFKT 
      SNKNRQLTL FDGKSEIMC CFMFQHGNC DYLITVLRG 
      MLKTIPAKR DKHLFIVIH DESLEAAQL DKSFAELNI 
      FQDPPPTLW KLVKSFHDR PYETTLETF AKVTDYVYR 
      SPEQRDIDD DQRELLHRS LTEYASSHS QGDYEVIAK 
      VPELPERKD YPRGRPLSA EQWKNLQNH EGKIEDVEQ 
      IKLMIFRGG VAPNLRYEV WKYLLDYFP WNSTQAERQ 
      KLLCEKNDE YYNMKLQWK RMTKVQEDN FSDYRERKN 
      LIEKDVNRT DRTMDFYAG DNNPNLQLL YDILMTYIM 
      YNFDLGYVQ GMSDLLSPI LHLLKNEVD AFWCFVGFM 
      NKISSNFDI DQAGMKEQL QNLHTLLGF IEPQLVNYL 
      DKHDSGNMF FCFRWLLVW FKRELSYDD VMRLWEVLW 
      TGLPCENFH LLVCVAILE TEKQALMEN NYGFTEILK 
      HINDLCGKL DVAAVLVKA EGIFHQIKE AEHLTDNIR 
      MILGLPLVG VQQCPEEIL STSPDSPDE DLYERVYDS 
      MQHVMT
    CDS sequence
    • >TC007868 RA
      atgggtgac agtttaacc gaatgcatg gagattttc 
      tcccaggat ggggtgcag ctcaaaaca gcacaggcg 
      tctttcgtc aactatttg aaccttgtg ggcacgtta 
      tatatcacc gaatatgca aattccaac tgcaggagg 
      tgtctcgag tggaagccc aatgatgta actgtggac 
      tcagacgtc caggaccag gaatgggct gttgttaac 
      actgtccag aaaagatcg aggacctac agtgaaagc 
      ctcccacca gactcaaca attaagccc aaactgttg 
      aaagtcaat ttcgttgat attaaaagc ttcaaaact 
      tcgaataaa aaccgtcaa ttgactttg tttgatggc 
      aaatcggaa attatgtgt tgtttcatg ttccaacac 
      gggaattgt gattattta attacggtt ttgagagga 
      atgctcaaa acaatacct gccaaaaga gacaagcat 
      ctttttatt gttatacat gacgagagt cttgaagca 
      gcacagctg gacaagtct ttcgctgag ttaaatatc 
      tttcaagac cctccaccc acgctgtgg aagcttgtg 
      aaaagtttc catgatagg ccgtacgaa accacacta 
      gagactttt gccaaagtc accgattat gtttacaga 
      agtccagaa caaagagat atcgacgac gatcaaaga 
      gaactgttg catcgaagt cttaccgag tatgcaagt 
      tcgcattct caaggagat tacgaagtc atagccaaa 
      gttcccgaa cttcccgaa cgaaaagac tatcccaga 
      ggccggccc ttgagcgcc gaacaatgg aaaaattta 
      caaaaccac gaaggtaaa atcgaggat gttgagcaa 
      atcaaactc atgatattc cgaggtgga gtggcgccc 
      aatttgcgc tacgaagtg tggaaatac ctcctggac 
      tatttcccc tggaacagc actcaagcc gaacgtcag 
      aaattatta tgcgaaaaa aacgacgag tattacaac 
      atgaagctc caatggaaa cgaatgacc aaagtccaa 
      gaagacaac ttctcggac tatcgcgag cgcaaaaat 
      ctgatcgaa aaagacgta aatcggacg gacaggacg 
      atggatttt tacgccggg gataacaac ccgaacttg 
      cagctgctc tacgatatt cttatgacc tacattatg 
      tacaacttt gacctaggc tacgtccaa ggcatgagc 
      gacttgctc tcgcccatt ttacacctc ctcaagaac 
      gaagtcgat gccttttgg tgctttgtg ggtttcatg 
      aataaaatt agctccaac ttcgacata gaccaggct 
      gggatgaaa gaacagctc cagaacttg cacactttg 
      ctcggcttc attgaacca caactcgtc aattacttg 
      gataagcac gactcagga aacatgttc ttctgtttc 
      cggtggctt ctagtttgg ttcaaacga gaattgagc 
      tatgacgat gtgatgcgt ttgtgggaa gtcctctgg 
      actggactt ccatgcgaa aatttccat ttgctagtc 
      tgtgttgcg attttagaa acggaaaaa caagcactc 
      atggaaaat aattacggc ttcaccgaa attctaaag 
      cacattaac gatctctgt ggaaaactg gacgtcgct 
      gctgtttta gtcaaagct gaggggatt tttcaccaa 
      atcaaagaa gcggaacat ttgacggac aatattcgc 
      atgattttg gggcttccc cttgtgggg gtgcaacaa 
      tgtcccgag gaaattcta agtacaagt cccgacagt 
      ccagatgag gatctctat gagcgagtt tatgactca 
      atgcaacac gttatgact tag
    mRNA sequence
    • >TC007868 RA
      cgtcatctg tcagttaaa cgtcaggga aaatgtcac 
      aaacgtcat taaaatcaa caatgtttg tttttgtgg 
      cttttgagg tttttggct tgtcgtttt tgccgttga 
      taaagacta tactttatc gttaaagtt tcgaatgat 
      ttttggtgc atcacatcc acgaacgat cgatcattt 
      gaaatttct tgtgaaagt gcttattgt tacaaatat 
      aaaaataac gtgaccatg actgctcat gtgcattgt 
      tacaattaa ttccaagtt ctaattaaa gcgtgatta 
      gaaaaaaat gggtgacag tttaaccga atgcatgga 
      gattttctc ccaggatgg ggtgcagct caaaacagc 
      acaggcgtc tttcgtcaa ctatttgaa ccttgtggg 
      cacgttata tatcaccga atatgcaaa ttccaactg 
      caggaggtg tctcgagtg gaagcccaa tgatgtaac 
      tgtggactc agacgtcca ggaccagga atgggctgt 
      tgttaacac tgtccagaa aagatcgag gacctacag 
      tgaaagcct cccaccaga ctcaacaat taagcccaa 
      actgttgaa agtcaattt cgttgatat taaaagctt 
      caaaacttc gaataaaaa ccgtcaatt gactttgtt 
      tgatggcaa atcggaaat tatgtgttg tttcatgtt 
      ccaacacgg gaattgtga ttatttaat tacggtttt 
      gagaggaat gctcaaaac aatacctgc caaaagaga 
      caagcatct ttttattgt tatacatga cgagagtct 
      tgaagcagc acagctgga caagtcttt cgctgagtt 
      aaatatctt tcaagaccc tccacccac gctgtggaa 
      gcttgtgaa aagtttcca tgataggcc gtacgaaac 
      cacactaga gacttttgc caaagtcac cgattatgt 
      ttacagaag tccagaaca aagagatat cgacgacga 
      tcaaagaga actgttgca tcgaagtct taccgagta 
      tgcaagttc gcattctca aggagatta cgaagtcat 
      agccaaagt tcccgaact tcccgaacg aaaagacta 
      tcccagagg ccggccctt gagcgccga acaatggaa 
      aaatttaca aaaccacga aggtaaaat cgaggatgt 
      tgagcaaat caaactcat gatattccg aggtggagt 
      ggcgcccaa tttgcgcta cgaagtgtg gaaatacct 
      cctggacta tttcccctg gaacagcac tcaagccga 
      acgtcagaa attattatg cgaaaaaaa cgacgagta 
      ttacaacat gaagctcca atggaaacg aatgaccaa 
      agtccaaga agacaactt ctcggacta tcgcgagcg 
      caaaaatct gatcgaaaa agacgtaaa tcggacgga 
      caggacgat ggattttta cgccgggga taacaaccc 
      gaacttgca gctgctcta cgatattct tatgaccta 
      cattatgta caactttga cctaggcta cgtccaagg 
      catgagcga cttgctctc gcccatttt acacctcct 
      caagaacga agtcgatgc cttttggtg ctttgtggg 
      tttcatgaa taaaattag ctccaactt cgacataga 
      ccaggctgg gatgaaaga acagctcca gaacttgca 
      cactttgct cggcttcat tgaaccaca actcgtcaa 
      ttacttgga taagcacga ctcaggaaa catgttctt 
      ctgtttccg gtggcttct agtttggtt caaacgaga 
      attgagcta tgacgatgt gatgcgttt gtgggaagt 
      cctctggac tggacttcc atgcgaaaa tttccattt 
      gctagtctg tgttgcgat tttagaaac ggaaaaaca 
      agcactcat ggaaaataa ttacggctt caccgaaat 
      tctaaagca cattaacga tctctgtgg aaaactgga 
      cgtcgctgc tgttttagt caaagctga ggggatttt 
      tcaccaaat caaagaagc ggaacattt gacggacaa 
      tattcgcat gattttggg gcttcccct tgtgggggt 
      gcaacaatg tcccgagga aattctaag tacaagtcc 
      cgacagtcc agatgagga tctctatga gcgagttta 
      tgactcaat gcaacacgt tatgactta gtagtgttt 
      gtacttttg taggaaggc taggtgtac aagagctca 
      ttatttaat gattgcaaa ttgttatta tgtgtttta 
      tcaattctt cgaatttta ttgctttta gaagagttt 
      tggcaacgt atattgtac ttttatggc aaataaata 
      ttttttatg aaa