Status
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Gene information
General information
TC007850 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1412951..1416085
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007850
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01273

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 4
    tropharium degenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007850 RA
      MDQPSEDSV PFYEEAANF ADNSEPPVS GHFYSVFIP 
HDVRQKGTQ TCDYIYKDK AEAMKMVKK TKKARFKHF 
EFFHDALKF ASVGAAETP NSPPVVNGS VKGLNSQVV 
NERLSPFKG PKPQDLVKL RKAIETGDV DTFRQAVWE 
NPRYLISSG DTPSILQEG FRYNALHVA AKAKSGILT 
EVILNTISN PKFVKLLYG DDVNENVES RIQVLLDLY 
LNTPDKGLN ETPLHFAAK FGAVDVVEV LVSFPECDK 
SLKNKYGQT PDMIICDRC DNIEGASII KKKIALHLE 
NSYYVPVLR AEDDSVAPV IGEPFSPGR SQVVNKDPL 
SPRLEIHAF AGPMNKEGA EQFRKVWKT PPRTSTPIK 
KRIDDPMDS VFSLRYTDP SKGLERLGQ KLANKFDVN 
WKEYWSFLD CFVDIGSDE GLALLENYL TAKIQSMDN 
SYLSPDSPE EASKKSLST IADLCLQMQ NCNINQSCE 
EESGFNSFL CVEKACNVF ANRIASEIY HRIFDDFSL 
QVSLLEMQT KQVELLILS YLDDYRFNK INFSQLHPR 
LGLLTSQKL CYFIEKSAR NNYIQNVNK LVTECVKAF 
DCFSSDDEA NYPKDNKPT SSKKQLICL MQNILNNFT 
QDSEPCLEL DEEQACRDA WMKTKECTC IFHFKKSRK 
TLGLSRNNS ARHSRLKTA KKLFFERID VETSSSDDD 
FYTPPESPS KIYDSDNEA DFSDVPLTL EVFVEGSQP 
TKTDNALYK ALKYSDCCI TPGKYPNIY RWFHNVGLY 
SEAERQSWP RSKIKTPPH TSLATPPRH ASSKATFQS 
PSSSSWLRI TGANSPRAS LQKLN
    CDS sequence
    • >TC007850 RA
      atggaccaa ccctccgaa gactctgtg ccgttctac 
gaagaagcg gcaaatttc gccgacaac agcgagccc 
cccgtctca ggccacttc tactcggtc tttatcccg 
cacgacgtg cgccagaag ggcacccaa acatgcgac 
tacatctac aaggacaag gccgaagcc atgaagatg 
gtcaagaag acgaagaaa gctcgcttc aagcatttc 
gaattcttc cacgatgcg ttgaaattc gcaagtgtg 
ggggctgcg gaaacgccg aattcccca ccggttgtc 
aacggatcg gtcaaaggt ttaaactcg caggttgtt 
aacgaaaga ctgagcccc ttcaagggc cccaaacct 
caggatctc gtcaagcta cgcaaagcg atagagacg 
ggggatgtg gacacgttc aggcaagcg gtgtgggag 
aaccccagg tatctgatc agcagtgga gacaccccg 
tccatcctc caggaaggt tttcgctac aatgcgctt 
cacgtggcg gcgaaagcg aaaagtgga attttgact 
gaagttatc ttgaatacg atttcaaat ccgaaattt 
gttaagctc ttgtatggt gatgacgtc aatgaaaac 
gttgaaagt cgtattcaa gtcctactt gacttgtat 
ttaaacact cccgacaag ggtctgaac gagacacca 
ctgcatttt gctgcgaaa ttcggtgct gtggacgtt 
gtcgaagtt ctagtgtca ttccctgaa tgtgataag 
agtttgaaa aataaatac ggacaaact cccgatatg 
attatttgt gatcgttgt gacaatatt gaaggagcg 
tctattatt aaaaagaaa attgctttg catttagaa 
aattcatac tacgttccg gtgcttcga gctgaagat 
gattcagta gcaccagtg attggtgag ccgttttca 
ccaggacga tcacaggtc gtcaataag gaccctctg 
agtccgcgg ctagaaatt cacgcattt gctggacca 
atgaacaag gaaggggcg gaacaattt cgcaaagtg 
tggaaaact ccgccccga acgagtaca ccaatcaag 
aagcgaatc gatgatcca atggattca gttttttcg 
ttgcgttac acagacccg tcgaaaggt ttagagcga 
ttgggtcaa aaactggcc aataaattt gacgttaac 
tggaaggaa tattggtcg tttttggat tgttttgtt 
gatattggc tctgatgaa ggtttagct ttgttggag 
aactatcta actgcgaaa attcaatca atggataac 
tcgtactta tcacccgat tcgccagaa gaggcgtca 
aaaaaatcc ttaagtacg attgcggac ctttgtctc 
caaatgcag aattgtaac attaaccaa agttgcgag 
gaagagtcc ggttttaat tcgttttta tgtgttgaa 
aaggcttgt aacgtgttt gcgaatcga atcgcgagt 
gagatttac catcggatt tttgatgat ttttctctg 
caagtttcc cttctcgaa atgcaaacc aaacaagtc 
gaattgttg attttaagt tacttggat gactaccgt 
tttaacaaa attaacttc tcgcaattg catccccgg 
ttaggtctc ttaaccagt caaaaattg tgttatttt 
atcgaaaaa tcggcgcgt aataattac attcaaaac 
gttaacaaa ttagtgacc gagtgtgtg aaagcgttc 
gattgtttt tcgtcggat gacgaggcc aattatccg 
aaagacaac aaaccaacg tcatcaaaa aaacaactg 
atttgtttg atgcaaaac attttaaat aatttcact 
caagatagt gagccgtgt ctggaactg gacgaggag 
caagcttgt cgagacgct tggatgaaa acaaaagag 
tgcacatgc atttttcat ttcaaaaag tcgcgaaaa 
acgctcggt ttgagccga aataacagt gcaaggcac 
tcgcgattg aagacggcc aagaaattg ttttttgaa 
cgcattgac gtggaaact tcatcgtct gatgatgat 
ttctacaca ccacctgaa agcccgagc aaaatttac 
gattcggac aatgaggcc gattttagt gatgttcct 
ctgactctt gaagtgttt gtcgaaggg tcacagccg 
accaagacc gataacgcc ctttataaa gctttaaaa 
tattccgat tgttgcata actccgggg aagtacccg 
aatatttat aggtggttc cataacgtc gggctctat 
tcagaggcg gagcgccag agttggccc cggtcgaag 
atcaaaact ccgcctcac acgtctttg gctacgccc 
ccgcgccac gcctcctcg aaagcaacg ttccaatcg 
ccttcttcc agttcgtgg ctccgaatc accggtgcg 
aactcacct cgagcaagt cttcaaaaa ctaaactaa
    mRNA sequence
    • >TC007850 RA
      cacgtgtca ctttgacat tcgtcggtt tagtttgca 
atttgcatt tgacgtgtt atttaaaat ttaaaacta 
gtttcttta ctctccttt tacaagaaa actccttac 
gtccaccca ccttgcgcg taaaatgcc catttcagc 
cctttcaca cacctaacc taagatgga ccaaccctc 
cgaagactc tgtgccgtt ctacgaaga agcggcaaa 
tttcgccga caacagcga gccccccgt ctcaggcca 
cttctactc ggtctttat cccgcacga cgtgcgcca 
gaagggcac ccaaacatg cgactacat ctacaagga 
caaggccga agccatgaa gatggtcaa gaagacgaa 
gaaagctcg cttcaagca tttcgaatt cttccacga 
tgcgttgaa attcgcaag tgtgggggc tgcggaaac 
gccgaattc cccaccggt tgtcaacgg atcggtcaa 
aggtttaaa ctcgcaggt tgttaacga aagactgag 
ccccttcaa gggccccaa acctcagga tctcgtcaa 
gctacgcaa agcgataga gacggggga tgtggacac 
gttcaggca agcggtgtg ggagaaccc caggtatct 
gatcagcag tggagacac cccgtccat cctccagga 
aggttttcg ctacaatgc gcttcacgt ggcggcgaa 
agcgaaaag tggaatttt gactgaagt tatcttgaa 
tacgatttc aaatccgaa atttgttaa gctcttgta 
tggtgatga cgtcaatga aaacgttga aagtcgtat 
tcaagtcct acttgactt gtatttaaa cactcccga 
caagggtct gaacgagac accactgca ttttgctgc 
gaaattcgg tgctgtgga cgttgtcga agttctagt 
gtcattccc tgaatgtga taagagttt gaaaaataa 
atacggaca aactcccga tatgattat ttgtgatcg 
ttgtgacaa tattgaagg agcgtctat tattaaaaa 
gaaaattgc tttgcattt agaaaattc atactacgt 
tccggtgct tcgagctga agatgattc agtagcacc 
agtgattgg tgagccgtt ttcaccagg acgatcaca 
ggtcgtcaa taaggaccc tctgagtcc gcggctaga 
aattcacgc atttgctgg accaatgaa caaggaagg 
ggcggaaca atttcgcaa agtgtggaa aactccgcc 
ccgaacgag tacaccaat caagaagcg aatcgatga 
tccaatgga ttcagtttt ttcgttgcg ttacacaga 
cccgtcgaa aggtttaga gcgattggg tcaaaaact 
ggccaataa atttgacgt taactggaa ggaatattg 
gtcgttttt ggattgttt tgttgatat tggctctga 
tgaaggttt agctttgtt ggagaacta tctaactgc 
gaaaattca atcaatgga taactcgta cttatcacc 
cgattcgcc agaagaggc gtcaaaaaa atccttaag 
tacgattgc ggacctttg tctccaaat gcagaattg 
taacattaa ccaaagttg cgaggaaga gtccggttt 
taattcgtt tttatgtgt tgaaaaggc ttgtaacgt 
gtttgcgaa tcgaatcgc gagtgagat ttaccatcg 
gatttttga tgatttttc tctgcaagt ttcccttct 
cgaaatgca aaccaaaca agtcgaatt gttgatttt 
aagttactt ggatgacta ccgttttaa caaaattaa 
cttctcgca attgcatcc ccggttagg tctcttaac 
cagtcaaaa attgtgtta ttttatcga aaaatcggc 
gcgtaataa ttacattca aaacgttaa caaattagt 
gaccgagtg tgtgaaagc gttcgattg tttttcgtc 
ggatgacga ggccaatta tccgaaaga caacaaacc 
aacgtcatc aaaaaaaca actgatttg tttgatgca 
aaacatttt aaataattt cactcaaga tagtgagcc 
gtgtctgga actggacga ggagcaagc ttgtcgaga 
cgcttggat gaaaacaaa agagtgcac atgcatttt 
tcatttcaa aaagtcgcg aaaaacgct cggtttgag 
ccgaaataa cagtgcaag gcactcgcg attgaagac 
ggccaagaa attgttttt tgaacgcat tgacgtgga 
aacttcatc gtctgatga tgatttcta cacaccacc 
tgaaagccc gagcaaaat ttacgattc ggacaatga 
ggccgattt tagtgatgt tcctctgac tcttgaagt 
gtttgtcga agggtcaca gccgaccaa gaccgataa 
cgcccttta taaagcttt aaaatattc cgattgttg 
cataactcc ggggaagta cccgaatat ttataggtg 
gttccataa cgtcgggct ctattcaga ggcggagcg 
ccagagttg gccccggtc gaagatcaa aactccgcc 
tcacacgtc tttggctac gcccccgcg ccacgcctc 
ctcgaaagc aacgttcca atcgccttc ttccagttc 
gtggctccg aatcaccgg tgcgaactc acctcgagc 
aagtcttca aaaactaaa ctaaggaga aacattttt 
atttgtgcc taaactaat t

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY