General information | |||
TC007850 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1412951..1416085 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC007850 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01273 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
tropharium degenerated - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (19 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (22 dpi)
- >TC007850 RA
MDQPSEDSV PFYEEAANF ADNSEPPVS GHFYSVFIP HDVRQKGTQ TCDYIYKDK AEAMKMVKK TKKARFKHF EFFHDALKF ASVGAAETP NSPPVVNGS VKGLNSQVV NERLSPFKG PKPQDLVKL RKAIETGDV DTFRQAVWE NPRYLISSG DTPSILQEG FRYNALHVA AKAKSGILT EVILNTISN PKFVKLLYG DDVNENVES RIQVLLDLY LNTPDKGLN ETPLHFAAK FGAVDVVEV LVSFPECDK SLKNKYGQT PDMIICDRC DNIEGASII KKKIALHLE NSYYVPVLR AEDDSVAPV IGEPFSPGR SQVVNKDPL SPRLEIHAF AGPMNKEGA EQFRKVWKT PPRTSTPIK KRIDDPMDS VFSLRYTDP SKGLERLGQ KLANKFDVN WKEYWSFLD CFVDIGSDE GLALLENYL TAKIQSMDN SYLSPDSPE EASKKSLST IADLCLQMQ NCNINQSCE EESGFNSFL CVEKACNVF ANRIASEIY HRIFDDFSL QVSLLEMQT KQVELLILS YLDDYRFNK INFSQLHPR LGLLTSQKL CYFIEKSAR NNYIQNVNK LVTECVKAF DCFSSDDEA NYPKDNKPT SSKKQLICL MQNILNNFT QDSEPCLEL DEEQACRDA WMKTKECTC IFHFKKSRK TLGLSRNNS ARHSRLKTA KKLFFERID VETSSSDDD FYTPPESPS KIYDSDNEA DFSDVPLTL EVFVEGSQP TKTDNALYK ALKYSDCCI TPGKYPNIY RWFHNVGLY SEAERQSWP RSKIKTPPH TSLATPPRH ASSKATFQS PSSSSWLRI TGANSPRAS LQKLN
- >TC007850 RA
atggaccaa ccctccgaa gactctgtg ccgttctac gaagaagcg gcaaatttc gccgacaac agcgagccc cccgtctca ggccacttc tactcggtc tttatcccg cacgacgtg cgccagaag ggcacccaa acatgcgac tacatctac aaggacaag gccgaagcc atgaagatg gtcaagaag acgaagaaa gctcgcttc aagcatttc gaattcttc cacgatgcg ttgaaattc gcaagtgtg ggggctgcg gaaacgccg aattcccca ccggttgtc aacggatcg gtcaaaggt ttaaactcg caggttgtt aacgaaaga ctgagcccc ttcaagggc cccaaacct caggatctc gtcaagcta cgcaaagcg atagagacg ggggatgtg gacacgttc aggcaagcg gtgtgggag aaccccagg tatctgatc agcagtgga gacaccccg tccatcctc caggaaggt tttcgctac aatgcgctt cacgtggcg gcgaaagcg aaaagtgga attttgact gaagttatc ttgaatacg atttcaaat ccgaaattt gttaagctc ttgtatggt gatgacgtc aatgaaaac gttgaaagt cgtattcaa gtcctactt gacttgtat ttaaacact cccgacaag ggtctgaac gagacacca ctgcatttt gctgcgaaa ttcggtgct gtggacgtt gtcgaagtt ctagtgtca ttccctgaa tgtgataag agtttgaaa aataaatac ggacaaact cccgatatg attatttgt gatcgttgt gacaatatt gaaggagcg tctattatt aaaaagaaa attgctttg catttagaa aattcatac tacgttccg gtgcttcga gctgaagat gattcagta gcaccagtg attggtgag ccgttttca ccaggacga tcacaggtc gtcaataag gaccctctg agtccgcgg ctagaaatt cacgcattt gctggacca atgaacaag gaaggggcg gaacaattt cgcaaagtg tggaaaact ccgccccga acgagtaca ccaatcaag aagcgaatc gatgatcca atggattca gttttttcg ttgcgttac acagacccg tcgaaaggt ttagagcga ttgggtcaa aaactggcc aataaattt gacgttaac tggaaggaa tattggtcg tttttggat tgttttgtt gatattggc tctgatgaa ggtttagct ttgttggag aactatcta actgcgaaa attcaatca atggataac tcgtactta tcacccgat tcgccagaa gaggcgtca aaaaaatcc ttaagtacg attgcggac ctttgtctc caaatgcag aattgtaac attaaccaa agttgcgag gaagagtcc ggttttaat tcgttttta tgtgttgaa aaggcttgt aacgtgttt gcgaatcga atcgcgagt gagatttac catcggatt tttgatgat ttttctctg caagtttcc cttctcgaa atgcaaacc aaacaagtc gaattgttg attttaagt tacttggat gactaccgt tttaacaaa attaacttc tcgcaattg catccccgg ttaggtctc ttaaccagt caaaaattg tgttatttt atcgaaaaa tcggcgcgt aataattac attcaaaac gttaacaaa ttagtgacc gagtgtgtg aaagcgttc gattgtttt tcgtcggat gacgaggcc aattatccg aaagacaac aaaccaacg tcatcaaaa aaacaactg atttgtttg atgcaaaac attttaaat aatttcact caagatagt gagccgtgt ctggaactg gacgaggag caagcttgt cgagacgct tggatgaaa acaaaagag tgcacatgc atttttcat ttcaaaaag tcgcgaaaa acgctcggt ttgagccga aataacagt gcaaggcac tcgcgattg aagacggcc aagaaattg ttttttgaa cgcattgac gtggaaact tcatcgtct gatgatgat ttctacaca ccacctgaa agcccgagc aaaatttac gattcggac aatgaggcc gattttagt gatgttcct ctgactctt gaagtgttt gtcgaaggg tcacagccg accaagacc gataacgcc ctttataaa gctttaaaa tattccgat tgttgcata actccgggg aagtacccg aatatttat aggtggttc cataacgtc gggctctat tcagaggcg gagcgccag agttggccc cggtcgaag atcaaaact ccgcctcac acgtctttg gctacgccc ccgcgccac gcctcctcg aaagcaacg ttccaatcg ccttcttcc agttcgtgg ctccgaatc accggtgcg aactcacct cgagcaagt cttcaaaaa ctaaactaa
- >TC007850 RA
cacgtgtca ctttgacat tcgtcggtt tagtttgca atttgcatt tgacgtgtt atttaaaat ttaaaacta gtttcttta ctctccttt tacaagaaa actccttac gtccaccca ccttgcgcg taaaatgcc catttcagc cctttcaca cacctaacc taagatgga ccaaccctc cgaagactc tgtgccgtt ctacgaaga agcggcaaa tttcgccga caacagcga gccccccgt ctcaggcca cttctactc ggtctttat cccgcacga cgtgcgcca gaagggcac ccaaacatg cgactacat ctacaagga caaggccga agccatgaa gatggtcaa gaagacgaa gaaagctcg cttcaagca tttcgaatt cttccacga tgcgttgaa attcgcaag tgtgggggc tgcggaaac gccgaattc cccaccggt tgtcaacgg atcggtcaa aggtttaaa ctcgcaggt tgttaacga aagactgag ccccttcaa gggccccaa acctcagga tctcgtcaa gctacgcaa agcgataga gacggggga tgtggacac gttcaggca agcggtgtg ggagaaccc caggtatct gatcagcag tggagacac cccgtccat cctccagga aggttttcg ctacaatgc gcttcacgt ggcggcgaa agcgaaaag tggaatttt gactgaagt tatcttgaa tacgatttc aaatccgaa atttgttaa gctcttgta tggtgatga cgtcaatga aaacgttga aagtcgtat tcaagtcct acttgactt gtatttaaa cactcccga caagggtct gaacgagac accactgca ttttgctgc gaaattcgg tgctgtgga cgttgtcga agttctagt gtcattccc tgaatgtga taagagttt gaaaaataa atacggaca aactcccga tatgattat ttgtgatcg ttgtgacaa tattgaagg agcgtctat tattaaaaa gaaaattgc tttgcattt agaaaattc atactacgt tccggtgct tcgagctga agatgattc agtagcacc agtgattgg tgagccgtt ttcaccagg acgatcaca ggtcgtcaa taaggaccc tctgagtcc gcggctaga aattcacgc atttgctgg accaatgaa caaggaagg ggcggaaca atttcgcaa agtgtggaa aactccgcc ccgaacgag tacaccaat caagaagcg aatcgatga tccaatgga ttcagtttt ttcgttgcg ttacacaga cccgtcgaa aggtttaga gcgattggg tcaaaaact ggccaataa atttgacgt taactggaa ggaatattg gtcgttttt ggattgttt tgttgatat tggctctga tgaaggttt agctttgtt ggagaacta tctaactgc gaaaattca atcaatgga taactcgta cttatcacc cgattcgcc agaagaggc gtcaaaaaa atccttaag tacgattgc ggacctttg tctccaaat gcagaattg taacattaa ccaaagttg cgaggaaga gtccggttt taattcgtt tttatgtgt tgaaaaggc ttgtaacgt gtttgcgaa tcgaatcgc gagtgagat ttaccatcg gatttttga tgatttttc tctgcaagt ttcccttct cgaaatgca aaccaaaca agtcgaatt gttgatttt aagttactt ggatgacta ccgttttaa caaaattaa cttctcgca attgcatcc ccggttagg tctcttaac cagtcaaaa attgtgtta ttttatcga aaaatcggc gcgtaataa ttacattca aaacgttaa caaattagt gaccgagtg tgtgaaagc gttcgattg tttttcgtc ggatgacga ggccaatta tccgaaaga caacaaacc aacgtcatc aaaaaaaca actgatttg tttgatgca aaacatttt aaataattt cactcaaga tagtgagcc gtgtctgga actggacga ggagcaagc ttgtcgaga cgcttggat gaaaacaaa agagtgcac atgcatttt tcatttcaa aaagtcgcg aaaaacgct cggtttgag ccgaaataa cagtgcaag gcactcgcg attgaagac ggccaagaa attgttttt tgaacgcat tgacgtgga aacttcatc gtctgatga tgatttcta cacaccacc tgaaagccc gagcaaaat ttacgattc ggacaatga ggccgattt tagtgatgt tcctctgac tcttgaagt gtttgtcga agggtcaca gccgaccaa gaccgataa cgcccttta taaagcttt aaaatattc cgattgttg cataactcc ggggaagta cccgaatat ttataggtg gttccataa cgtcgggct ctattcaga ggcggagcg ccagagttg gccccggtc gaagatcaa aactccgcc tcacacgtc tttggctac gcccccgcg ccacgcctc ctcgaaagc aacgttcca atcgccttc ttccagttc gtggctccg aatcaccgg tgcgaactc acctcgagc aagtcttca aaaactaaa ctaaggaga aacattttt atttgtgcc taaactaat t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
