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Gene information
General information
TC007850 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1412951..1416085
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007850
    iBeetle screen iB_01273

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 4
    tropharium degenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007850 RA
      MDQPSEDSV PFYEEAANF ADNSEPPVS GHFYSVFIP 
      HDVRQKGTQ TCDYIYKDK AEAMKMVKK TKKARFKHF 
      EFFHDALKF ASVGAAETP NSPPVVNGS VKGLNSQVV 
      NERLSPFKG PKPQDLVKL RKAIETGDV DTFRQAVWE 
      NPRYLISSG DTPSILQEG FRYNALHVA AKAKSGILT 
      EVILNTISN PKFVKLLYG DDVNENVES RIQVLLDLY 
      LNTPDKGLN ETPLHFAAK FGAVDVVEV LVSFPECDK 
      SLKNKYGQT PDMIICDRC DNIEGASII KKKIALHLE 
      NSYYVPVLR AEDDSVAPV IGEPFSPGR SQVVNKDPL 
      SPRLEIHAF AGPMNKEGA EQFRKVWKT PPRTSTPIK 
      KRIDDPMDS VFSLRYTDP SKGLERLGQ KLANKFDVN 
      WKEYWSFLD CFVDIGSDE GLALLENYL TAKIQSMDN 
      SYLSPDSPE EASKKSLST IADLCLQMQ NCNINQSCE 
      EESGFNSFL CVEKACNVF ANRIASEIY HRIFDDFSL 
      QVSLLEMQT KQVELLILS YLDDYRFNK INFSQLHPR 
      LGLLTSQKL CYFIEKSAR NNYIQNVNK LVTECVKAF 
      DCFSSDDEA NYPKDNKPT SSKKQLICL MQNILNNFT 
      QDSEPCLEL DEEQACRDA WMKTKECTC IFHFKKSRK 
      TLGLSRNNS ARHSRLKTA KKLFFERID VETSSSDDD 
      FYTPPESPS KIYDSDNEA DFSDVPLTL EVFVEGSQP 
      TKTDNALYK ALKYSDCCI TPGKYPNIY RWFHNVGLY 
      SEAERQSWP RSKIKTPPH TSLATPPRH ASSKATFQS 
      PSSSSWLRI TGANSPRAS LQKLN
    CDS sequence
    • >TC007850 RA
      atggaccaa ccctccgaa gactctgtg ccgttctac 
      gaagaagcg gcaaatttc gccgacaac agcgagccc 
      cccgtctca ggccacttc tactcggtc tttatcccg 
      cacgacgtg cgccagaag ggcacccaa acatgcgac 
      tacatctac aaggacaag gccgaagcc atgaagatg 
      gtcaagaag acgaagaaa gctcgcttc aagcatttc 
      gaattcttc cacgatgcg ttgaaattc gcaagtgtg 
      ggggctgcg gaaacgccg aattcccca ccggttgtc 
      aacggatcg gtcaaaggt ttaaactcg caggttgtt 
      aacgaaaga ctgagcccc ttcaagggc cccaaacct 
      caggatctc gtcaagcta cgcaaagcg atagagacg 
      ggggatgtg gacacgttc aggcaagcg gtgtgggag 
      aaccccagg tatctgatc agcagtgga gacaccccg 
      tccatcctc caggaaggt tttcgctac aatgcgctt 
      cacgtggcg gcgaaagcg aaaagtgga attttgact 
      gaagttatc ttgaatacg atttcaaat ccgaaattt 
      gttaagctc ttgtatggt gatgacgtc aatgaaaac 
      gttgaaagt cgtattcaa gtcctactt gacttgtat 
      ttaaacact cccgacaag ggtctgaac gagacacca 
      ctgcatttt gctgcgaaa ttcggtgct gtggacgtt 
      gtcgaagtt ctagtgtca ttccctgaa tgtgataag 
      agtttgaaa aataaatac ggacaaact cccgatatg 
      attatttgt gatcgttgt gacaatatt gaaggagcg 
      tctattatt aaaaagaaa attgctttg catttagaa 
      aattcatac tacgttccg gtgcttcga gctgaagat 
      gattcagta gcaccagtg attggtgag ccgttttca 
      ccaggacga tcacaggtc gtcaataag gaccctctg 
      agtccgcgg ctagaaatt cacgcattt gctggacca 
      atgaacaag gaaggggcg gaacaattt cgcaaagtg 
      tggaaaact ccgccccga acgagtaca ccaatcaag 
      aagcgaatc gatgatcca atggattca gttttttcg 
      ttgcgttac acagacccg tcgaaaggt ttagagcga 
      ttgggtcaa aaactggcc aataaattt gacgttaac 
      tggaaggaa tattggtcg tttttggat tgttttgtt 
      gatattggc tctgatgaa ggtttagct ttgttggag 
      aactatcta actgcgaaa attcaatca atggataac 
      tcgtactta tcacccgat tcgccagaa gaggcgtca 
      aaaaaatcc ttaagtacg attgcggac ctttgtctc 
      caaatgcag aattgtaac attaaccaa agttgcgag 
      gaagagtcc ggttttaat tcgttttta tgtgttgaa 
      aaggcttgt aacgtgttt gcgaatcga atcgcgagt 
      gagatttac catcggatt tttgatgat ttttctctg 
      caagtttcc cttctcgaa atgcaaacc aaacaagtc 
      gaattgttg attttaagt tacttggat gactaccgt 
      tttaacaaa attaacttc tcgcaattg catccccgg 
      ttaggtctc ttaaccagt caaaaattg tgttatttt 
      atcgaaaaa tcggcgcgt aataattac attcaaaac 
      gttaacaaa ttagtgacc gagtgtgtg aaagcgttc 
      gattgtttt tcgtcggat gacgaggcc aattatccg 
      aaagacaac aaaccaacg tcatcaaaa aaacaactg 
      atttgtttg atgcaaaac attttaaat aatttcact 
      caagatagt gagccgtgt ctggaactg gacgaggag 
      caagcttgt cgagacgct tggatgaaa acaaaagag 
      tgcacatgc atttttcat ttcaaaaag tcgcgaaaa 
      acgctcggt ttgagccga aataacagt gcaaggcac 
      tcgcgattg aagacggcc aagaaattg ttttttgaa 
      cgcattgac gtggaaact tcatcgtct gatgatgat 
      ttctacaca ccacctgaa agcccgagc aaaatttac 
      gattcggac aatgaggcc gattttagt gatgttcct 
      ctgactctt gaagtgttt gtcgaaggg tcacagccg 
      accaagacc gataacgcc ctttataaa gctttaaaa 
      tattccgat tgttgcata actccgggg aagtacccg 
      aatatttat aggtggttc cataacgtc gggctctat 
      tcagaggcg gagcgccag agttggccc cggtcgaag 
      atcaaaact ccgcctcac acgtctttg gctacgccc 
      ccgcgccac gcctcctcg aaagcaacg ttccaatcg 
      ccttcttcc agttcgtgg ctccgaatc accggtgcg 
      aactcacct cgagcaagt cttcaaaaa ctaaactaa
    mRNA sequence
    • >TC007850 RA
      cacgtgtca ctttgacat tcgtcggtt tagtttgca 
      atttgcatt tgacgtgtt atttaaaat ttaaaacta 
      gtttcttta ctctccttt tacaagaaa actccttac 
      gtccaccca ccttgcgcg taaaatgcc catttcagc 
      cctttcaca cacctaacc taagatgga ccaaccctc 
      cgaagactc tgtgccgtt ctacgaaga agcggcaaa 
      tttcgccga caacagcga gccccccgt ctcaggcca 
      cttctactc ggtctttat cccgcacga cgtgcgcca 
      gaagggcac ccaaacatg cgactacat ctacaagga 
      caaggccga agccatgaa gatggtcaa gaagacgaa 
      gaaagctcg cttcaagca tttcgaatt cttccacga 
      tgcgttgaa attcgcaag tgtgggggc tgcggaaac 
      gccgaattc cccaccggt tgtcaacgg atcggtcaa 
      aggtttaaa ctcgcaggt tgttaacga aagactgag 
      ccccttcaa gggccccaa acctcagga tctcgtcaa 
      gctacgcaa agcgataga gacggggga tgtggacac 
      gttcaggca agcggtgtg ggagaaccc caggtatct 
      gatcagcag tggagacac cccgtccat cctccagga 
      aggttttcg ctacaatgc gcttcacgt ggcggcgaa 
      agcgaaaag tggaatttt gactgaagt tatcttgaa 
      tacgatttc aaatccgaa atttgttaa gctcttgta 
      tggtgatga cgtcaatga aaacgttga aagtcgtat 
      tcaagtcct acttgactt gtatttaaa cactcccga 
      caagggtct gaacgagac accactgca ttttgctgc 
      gaaattcgg tgctgtgga cgttgtcga agttctagt 
      gtcattccc tgaatgtga taagagttt gaaaaataa 
      atacggaca aactcccga tatgattat ttgtgatcg 
      ttgtgacaa tattgaagg agcgtctat tattaaaaa 
      gaaaattgc tttgcattt agaaaattc atactacgt 
      tccggtgct tcgagctga agatgattc agtagcacc 
      agtgattgg tgagccgtt ttcaccagg acgatcaca 
      ggtcgtcaa taaggaccc tctgagtcc gcggctaga 
      aattcacgc atttgctgg accaatgaa caaggaagg 
      ggcggaaca atttcgcaa agtgtggaa aactccgcc 
      ccgaacgag tacaccaat caagaagcg aatcgatga 
      tccaatgga ttcagtttt ttcgttgcg ttacacaga 
      cccgtcgaa aggtttaga gcgattggg tcaaaaact 
      ggccaataa atttgacgt taactggaa ggaatattg 
      gtcgttttt ggattgttt tgttgatat tggctctga 
      tgaaggttt agctttgtt ggagaacta tctaactgc 
      gaaaattca atcaatgga taactcgta cttatcacc 
      cgattcgcc agaagaggc gtcaaaaaa atccttaag 
      tacgattgc ggacctttg tctccaaat gcagaattg 
      taacattaa ccaaagttg cgaggaaga gtccggttt 
      taattcgtt tttatgtgt tgaaaaggc ttgtaacgt 
      gtttgcgaa tcgaatcgc gagtgagat ttaccatcg 
      gatttttga tgatttttc tctgcaagt ttcccttct 
      cgaaatgca aaccaaaca agtcgaatt gttgatttt 
      aagttactt ggatgacta ccgttttaa caaaattaa 
      cttctcgca attgcatcc ccggttagg tctcttaac 
      cagtcaaaa attgtgtta ttttatcga aaaatcggc 
      gcgtaataa ttacattca aaacgttaa caaattagt 
      gaccgagtg tgtgaaagc gttcgattg tttttcgtc 
      ggatgacga ggccaatta tccgaaaga caacaaacc 
      aacgtcatc aaaaaaaca actgatttg tttgatgca 
      aaacatttt aaataattt cactcaaga tagtgagcc 
      gtgtctgga actggacga ggagcaagc ttgtcgaga 
      cgcttggat gaaaacaaa agagtgcac atgcatttt 
      tcatttcaa aaagtcgcg aaaaacgct cggtttgag 
      ccgaaataa cagtgcaag gcactcgcg attgaagac 
      ggccaagaa attgttttt tgaacgcat tgacgtgga 
      aacttcatc gtctgatga tgatttcta cacaccacc 
      tgaaagccc gagcaaaat ttacgattc ggacaatga 
      ggccgattt tagtgatgt tcctctgac tcttgaagt 
      gtttgtcga agggtcaca gccgaccaa gaccgataa 
      cgcccttta taaagcttt aaaatattc cgattgttg 
      cataactcc ggggaagta cccgaatat ttataggtg 
      gttccataa cgtcgggct ctattcaga ggcggagcg 
      ccagagttg gccccggtc gaagatcaa aactccgcc 
      tcacacgtc tttggctac gcccccgcg ccacgcctc 
      ctcgaaagc aacgttcca atcgccttc ttccagttc 
      gtggctccg aatcaccgg tgcgaactc acctcgagc 
      aagtcttca aaaactaaa ctaaggaga aacattttt 
      atttgtgcc taaactaat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY