| General information | |||
| TC007849 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1403001..1411907 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC007849 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01272 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
remarks: later than schedule, entire repetition development delayed, hours 60
Pupal morphology
remarks: later than schedule, entire repetition development delayed, hours 60
Adult morphology
remarks: later than schedule, entire repetition development delayed, hours 60
- >TC007849 RA
MNCLLVLLL LCHYFVENG TAEHEKYRR TIHGYEDPP SNGKKTFII ERNFETGGH NVEKRSIVS TDDSKIIQK INYLNDTHK QLMVHWVGE GSNVIICLA RHSSLETLA GDVKVGPSA VYISYDYGD TYVNKTDLF KLSDGSYAT LEKFYNHPK YNTHFVFAD VNHKVLYVT ANHGRDIKR IDLDFTPSD VSFHELQPS VFVVLDKVD HLHKLWVTE DFGNSFRAT QDFVKATYW IKENDLRHT LLIQRMEPS GYNAILSSS DLFSNRTVM VQATGIKEF FVKGDYLFT TKLSSKGVL ELYVSYKLG KKIKCVFDT TLEIRSYFI VDVTSNRAL VAVSHADTV SHLYVSENL DNSEGVVKF TLSLEDVLC YFPNSTWHD TWIHHVSEE AFADVYKVE GLSGIYIAS RVLTKPQGN NLGPQHLGS VITFDHGRS WRLIQAPFR DVEGQPTSC SVQQNCSLH LSQKFSQLY PDTRSISIL SSKSAPGLL IATGVLGKS LKGHYGVYI SSDAGQTWR QTLRDLYFF NMGDHGGIL TAVKYFKLR GETRHVLYS TDEGETWKQ AAFHDQNIR LYGLMTEPG ENTTVFTMF GSLPEEHQW IIIKLDLIK AFAYNCSKD DYKPWSPSQ SDENRSYIP CVLGQQVTY HRRMPHANC YNGEDFDVP ISMTPCGCD IYDYECDFG FIRSEKPGN CVRNTSITS DPYRIPATC KPGKFYKRT KGYRKIQGD VCVDGFENH YMPEEIPCP FNEVDDFLL FAQRERISR FNLVTRKLE ELPVKNLKN VIAIDFDMR NNCVYWADI ALDTIGRQC LNDSEPEIL VSSDLASIE GMALDWISN TLYFVDGIR SKIELIRTD VNHSGRMRR TVLKGDVLK KPRGIALHP KAGYMFWTD WSVERPSVN RADLDGSNV VKLFGKPRV EWPNGITID YIAERIYWV DARQDYIAS SDLHGDFLK IIISQNDVV SHPFAVAVF KNTMYWDDW KRNAIFSAD KDTYHGVEI LQKQLPGLM DLKVYAHGL QVGTNACTA SKCSYICVS KPKKNSSCL CPDGMEYNN GRCLCPGHV PPLANFTCP PVQNTCSSN HFTCRNGLC VPKGWKCDG EDDCGDSSD EAHCGMQSC PPNYFVCGD GKCLPQYWK CDYDYDCSD GSDEVDCPK QNCTDGQFT CKNGRCIAE KWKCDGEND CRDGSDELN CEPVEPHVC KSEEFQCKV GGVKCIPST WQCDGEKDC RDGSDEMNC KNNTCLDIQ FSCGEPNNR CIYKTWVCD GDKDCPNGS DEVNCTTPA IPDTSDATD QFIPKNGTC QDWMFRCQN QRCIPYWWK CDQADDCGD GSDELGCFP SHAPTPKST TTPPVTTCG NNQFECMGG TCIFSSWVC DGMEDCVGG EDERHCEGV RNCTKDEFK CHVDGSCIP LAAVCNRQP DCPDLTDES LCDHNLPNG PATPSCSKG YFPCDGGSC YPLAALCDG RVDCRDGYD ELNCTKTSR VYQVLRMGV DERSINETS LLLYWWITD PPVGKLEFL PSISKVGEN VWENKTWMK DMEYKFTNL QPATQYNMT IYVRVQNTS KVFLPAKYF VAFTAESIP SEPWNVTAV QTNGSHVLL SWNKPTHPN GQIVNYEIC WYPPNPPIK LKLNGNETA HLLSAYFEP DKYYNFYVI AHNHKYESK RSKVTTLKF DGDAKLHAV ENLRVDKKS ETSVTLSWK YNKTVEGFA VAIIPPNDY PRMLPRVTN TQSITVDKL APGTRYTFK VNAYKNAFT GPEVDIIVV TDGVMLPEV PSLQGKLVK EVGTSVKLS WGVPKDSRK VKWTYGVYY GIDADQLYE KSRFETTDL TTTINDLKA CEYYTFMVG VVGPYGQGP LSKSLQIST FTNKKAPPT NLNVKEVEH NPLKMMITW VPSCPTIAE PIGHMIRVY ERTTGKISS YGKPPGNYS HVLNVKYGA IYDISVATR EVDAIYTPN VTYYAPPIL PPHEVKTTI EQNGSFVVD WQEADVPKA VGPFKYEVL VSEGNTLDE ATARRFRVD KPPFIYTNS SSNMYTFAV QIVADSGYK SITSEKLSA TVLELPTTM GGTNLWLIL VLTILSIVV VVTLGVFIV KHRRLQNSF TRFANSHYD TRSGAATFD DNGLEEEES PQITGFSDD EPLVIA
- >TC007849 RA
atgaactgt ctccttgtt ttgttattg ttgtgccat tatttcgtg gaaaatggc acagccgaa cacgaaaag tacagacga acgatacat gggtacgag gatccgcca agcaatggc aaaaaaacc ttcattatt gagcgaaat ttcgaaacc gggggacac aacgtcgaa aaaagatct atcgtgtcc actgatgac agtaaaatc atacagaag ataaattat ctgaatgac acacacaaa caactgatg gtgcattgg gtgggcgaa ggtagcaac gttattatt tgcctagca cgtcactcg tctcttgaa acattggct ggggatgta aaagttgga ccatcggcc gtttatatt tcctacgat tatggcgac acttatgtc aacaaaact gatcttttt aaattatcg gacgggtct tatgccact ttggaaaag ttttacaac catccgaaa tacaacaca cattttgtt tttgccgac gtaaaccat aaagtactc tacgtaacg gcgaatcac ggccgtgac ataaaacgc atcgacttg gatttcaca ccgagcgac gtttcgttt cacgaactg caaccgtca gtgtttgtc gttttggat aaagtcgat catctgcat aaattatgg gtgactgaa gatttcggt aatagtttt cgagcgaca caggatttc gtcaaagcc acttattgg atcaaagag aatgatttg agacacact ttattgata caaaggatg gaaccgagt ggttataac gcgatttta tcatcgagc gatttattc tcgaatcga acggttatg gtccaagcc accggtatt aaggagttc ttcgtcaaa ggcgattat ctcttcacg accaaatta tcgagcaaa ggagtgctc gagttgtac gtttcgtat aaacttggg aagaaaatt aagtgtgtt ttcgatacg actttagag attcggagc tatttcatt gttgatgtt acttcgaat cgggctctt gtggcagtg agtcatgcc gatactgtc tcacatttg tacgtttcg gagaatttg gacaacagt gagggagtt gttaaattc acactgtca ctagaagac gtcctttgc tattttccg aacagtaca tggcacgat acttggatc caccacgtc tcagaggaa gctttcgcc gatgtttac aaagtcgaa ggtctctca ggcatttac atcgcctca cgtgtctta accaaaccc cagggtaac aatctcggt ccgcaacac ttaggttcg gttataacc ttcgaccac ggccgatct tggcgtctg atccaagcc cctttccgt gatgtcgaa ggacaaccg acttcctgc tccgtccaa cagaattgc tccctccat ttgagccaa aagttcagt caattgtac cccgatact agatctatt agtattcta tcgagtaaa tcggcgcct gggctttta atcgcaaca ggtgttttg ggaaaatca cttaaagga cattacggt gtttatata agtagtgat gcgggacag acgtggaga cagactttg agggatttg tatttcttt aatatgggt gaccatggt gggatttta acagcggtt aagtatttt aaattgagg ggggagacg aggcatgtt ttgtattca acggacgag ggagagaca tggaaacag gcggctttt cacgatcag aatattcgc ttgtatggg cttatgacc gaaccgggg gaaaacacg acagtgttt acgatgttt ggctccttg ccggaggaa catcagtgg attattatc aagctggat ttaataaag gccttcgcc tataactgc tcaaaagac gactacaag ccgtggtcc ccgagccaa agcgacgaa aaccgcagt tacatccct tgcgttttg ggccaacaa gtcacctac caccgtcgc atgccccac gctaattgt tacaacggt gaagatttc gacgtcccc atctcaatg acaccgtgc ggttgcgat atttacgac tacgagtgt gattttggt ttcatccgt tcggaaaaa cccggcaat tgcgtccgc aacacttca atcaccagc gatccttac cgtattccc gccacttgc aaaccgggc aaattttac aaacggacg aaaggctac cgaaaaatc caaggcgat gtttgcgtc gacggcttt gagaatcat tacatgccg gaggaaatc ccctgtccg tttaacgaa gttgacgat ttcctcttg tttgcgcaa cgggagaga atcagtcgg tttaatctc gtgacaagg aaactcgaa gagcttccg gttaagaat ttgaaaaat gtgatcgcg attgatttc gatatgcgg aataattgc gtgtattgg gccgatatt gcattggat acgatcgga cggcagtgt ttgaacgac agtgagccg gagattttg gtttcgagt gatttggcc tcgattgaa ggcatggct ttggattgg atttccaat actttgtat tttgtcgat gggattcgg tcaaagatt gagttgatc aggactgat gttaatcac agtgggaga atgcgtaga actgtgtta aaaggcgac gttttgaag aaaccacga gggattgcc ttgcacccc aaagctggg tacatgttc tggactgac tggtcggtg gaacggcct tcggtcaac cgagctgac ttggacggt tccaatgtg gttaaactg ttcggcaaa cctcgggtc gaatggccc aatggtata accatcgac tacatcgcc gaacgcatc tactgggtg gacgcccgt caagactac atcgcctcc agtgacttg cacggcgat ttcctcaag ataataatc tcgcaaaac gacgttgtc tcacacccg tttgccgtc gcagttttc aaaaacaca atgtattgg gacgattgg aaacgtaac gcgattttt agcgccgat aaggacacc tatcacggc gtggaaatc ctccagaag caattgcca ggtctcatg gaccttaaa gtctacgcg cacggcctc caagtgggc acgaacgcc tgcactgcc tccaaatgc tcatacatt tgtgtcagc aaaccgaaa aagaactcg tcttgttta tgccccgat ggcatggaa tacaacaac ggacggtgt ctctgcccg gggcatgtg cccccattg gctaacttc acctgtccg ccggtgcaa aacacctgc agttcgaat catttcacg tgtcggaac ggtctatgt gtgcccaaa ggctggaag tgtgatggt gaagatgat tgtggcgat tcctccgat gaggcgcac tgtggcatg cagagttgc ccaccgaat tatttcgtt tgtggtgat ggcaagtgt ttgccacag tattggaag tgtgactat gactatgat tgttcggac gggtcggat gaagtggat tgtccgaaa cagaactgc accgatggg caatttacg tgtaagaat ggccggtgt attgcagag aagtggaag tgtgatggg gaaaatgat tgtcgggat gggtcagat gagttgaat tgtgaaccg gtggagcca cacgtgtgt aaaagtgag gagttccag tgcaaggtt ggtggtgtt aaatgtata ccatcgacg tggcaatgc gacggtgaa aaggattgt cgagatggg tcagatgaa atgaattgc aaaaataat acgtgtctc gatattcaa ttttcgtgt ggtgaaccc aacaatcgt tgtatttac aaaacatgg gtgtgtgac ggtgataaa gactgtccc aatggcagt gacgaagtc aattgtacg actcctgca atccctgat acaagcgat gccactgat cagtttata cccaaaaac gggacgtgt caggattgg atgttccgt tgccagaac cagcgctgc atcccctat tggtggaag tgcgatcaa gccgacgat tgcggcgac ggcagcgat gaacttggt tgtttccca agccatgcc ccgacgccc aagtccaca accacaccc cctgttacg acttgtggt aacaaccaa ttcgagtgt atgggcggg acttgtatc ttttcgtcg tgggtgtgc gacggtatg gaggattgt gtgggcggt gaagatgaa cgacattgt gaaggtgta cgtaattgc accaaagac gagttcaaa tgtcacgtg gatggaagt tgtataccg ttggcagcg gtttgtaat cgacagccg gattgtccc gatttgaca gatgagtct ttgtgtgat cataatcta ccgaacgga ccagccacg ccctcgtgt tcaaaaggg tacttccct tgtgatgga ggtagttgt tacccattg gctgcattg tgcgatggg cgtgttgat tgtcgcgac ggttacgac gaattaaat tgcacgaaa acatcaagg gtgtatcaa gtgttacga atgggcgtg gacgaacgg agtattaac gagacgagt cttttgctc tactggtgg atcacagac ccgccagtc ggtaaatta gagttctta ccgtcgatt agtaaagtg ggagagaat gtttgggaa aacaaaacg tggatgaag gacatggag tataaattt accaatttg caaccggcg actcaatac aatatgacg atttatgtc agggttcaa aatacgagc aaagtgttt ttacctgcg aaatatttc gtggctttt acggctgaa agcattcca agtgagcct tggaacgtg acagctgtt caaacgaac ggttctcat gttcttttg tcttggaat aaaccaacg catcccaac ggccaaatc gtcaattac gagatatgt tggtaccca ccgaaccca ccaatcaaa ttgaagctg aacggtaac gaaaccgcc catttgctc tccgcctac ttcgagccc gataaatat tacaacttt tatgtaatc gcccacaat cacaagtat gaaagtaag cgctcaaaa gtcacgact ttgaaattc gatggcgac gctaaactc cacgctgtc gaaaattta agagtggac aaaaaatcc gaaactagc gtcacacta tcgtggaaa tataacaaa acagttgaa ggtttcgcc gttgcgata attccaccc aatgactat ccgcgaatg ttaccacgt gtcaccaac acacaatcc atcactgtc gataagtta gcgcccggt acccgctac acgttcaag gttaacgct tacaaaaat gcattcacc ggccccgaa gttgacata atcgtggta actgacggc gtcatgtta ccagaagtg ccgtcactt caaggaaaa ttggtcaaa gaagtcgga acttcggtc aaattgagt tggggagtt ccgaaagat tcgagaaaa gtcaagtgg acgtatggt gtttattat ggaattgac gccgatcag ttatatgaa aaatcgaga tttgaaaca actgatttg acgacaacc atcaacgat ttaaaagct tgcgagtat tatactttc atggtcgga gttgtggga ccgtatggt caaggccca ctatcaaag agcttacaa atcagtacg tttacgaat aagaaggca ccgccgaca aatctgaac gtcaaggaa gtggaacac aatccgttg aaaatgatg atcacgtgg gtgccttcg tgtccgacc atcgccgaa cccatcggc catatgatc cgtgtctat gaaagaaca accggtaaa atatcctcg tatggcaaa ccacctggg aattacagt cacgtcttg aatgtaaaa tacggcgct atctatgac ataagcgtg gcaacacgc gaagtggac gccatctac acacccaac gtgacttat tacgccccg cccattttg cctccacac gaagtgaaa acaaccatt gaacagaac ggtagtttt gttgtcgat tggcaagaa gccgacgtt cccaaggcc gtagggccg tttaaatac gaagttttg gtttctgaa ggaaatact ctggacgag gcaacggcg aggcgtttt cgagtggac aaaccacca tttatctac actaacagt tcatcgaat atgtacact ttcgcggtc caaatcgtg gctgatagt gggtacaaa tcgatcaca tcggagaag ttgagtgca acggttttg gaactgccc acgaccatg ggtggtacc aatctctgg ttgattttg gtgctcacg attttgtca atagtcgtg gtggtaaca ctgggtgtg tttatagtg aaacatcgc cgcttacag aatagtttc acgcgattt gcaaattcg cattatgat acgcggtca ggggcggcg acttttgac gataatgga ttagaggag gaggaaagt ccgcaaata acagggttt tcggatgac gagccgctc gttatcgcg tag
- >TC007849 RA
tttttttga cacatgacg cacttcttt cagtcatca gctgacagt tgttgtgaa tgggtgctc aaaaatgaa ctgtctcct tgttttgtt attgttgtg ccattattt cgtggaaaa tggcacagc cgaacacga aaagtacag acgaacgat acatgggta cgaggatcc gccaagcaa tggcaaaaa aaccttcat tattgagcg aaatttcga aaccggggg acacaacgt cgaaaaaag atctatcgt gtccactga tgacagtaa aatcataca gaagataaa ttatctgaa tgacacaca caaacaact gatggtgca ttgggtggg cgaaggtag caacgttat tatttgcct agcacgtca ctcgtctct tgaaacatt ggctgggga tgtaaaagt tggaccatc ggccgttta tatttccta cgattatgg cgacactta tgtcaacaa aactgatct ttttaaatt atcggacgg gtcttatgc cactttgga aaagtttta caaccatcc gaaatacaa cacacattt tgtttttgc cgacgtaaa ccataaagt actctacgt aacggcgaa tcacggccg tgacataaa acgcatcga cttggattt cacaccgag cgacgtttc gtttcacga actgcaacc gtcagtgtt tgtcgtttt ggataaagt cgatcatct gcataaatt atgggtgac tgaagattt cggtaatag ttttcgagc gacacagga tttcgtcaa agccactta ttggatcaa agagaatga tttgagaca cactttatt gatacaaag gatggaacc gagtggtta taacgcgat tttatcatc gagcgattt attctcgaa tcgaacggt tatggtcca agccaccgg tattaagga gttcttcgt caaaggcga ttatctctt cacgaccaa attatcgag caaaggagt gctcgagtt gtacgtttc gtataaact tgggaagaa aattaagtg tgttttcga tacgacttt agagattcg gagctattt cattgttga tgttacttc gaatcgggc tcttgtggc agtgagtca tgccgatac tgtctcaca tttgtacgt ttcggagaa tttggacaa cagtgaggg agttgttaa attcacact gtcactaga agacgtcct ttgctattt tccgaacag tacatggca cgatacttg gatccacca cgtctcaga ggaagcttt cgccgatgt ttacaaagt cgaaggtct ctcaggcat ttacatcgc ctcacgtgt cttaaccaa accccaggg taacaatct cggtccgca acacttagg ttcggttat aaccttcga ccacggccg atcttggcg tctgatcca agccccttt ccgtgatgt cgaaggaca accgacttc ctgctccgt ccaacagaa ttgctccct ccatttgag ccaaaagtt cagtcaatt gtaccccga tactagatc tattagtat tctatcgag taaatcggc gcctgggct tttaatcgc aacaggtgt tttgggaaa atcacttaa aggacatta cggtgttta tataagtag tgatgcggg acagacgtg gagacagac tttgaggga tttgtattt ctttaatat gggtgacca tggtgggat tttaacagc ggttaagta ttttaaatt gagggggga gacgaggca tgttttgta ttcaacgga cgagggaga gacatggaa acaggcggc ttttcacga tcagaatat tcgcttgta tgggcttat gaccgaacc gggggaaaa cacgacagt gtttacgat gtttggctc cttgccgga ggaacatca gtggattat tatcaagct ggatttaat aaaggcctt cgcctataa ctgctcaaa agacgacta caagccgtg gtccccgag ccaaagcga cgaaaaccg cagttacat cccttgcgt tttgggcca acaagtcac ctaccaccg tcgcatgcc ccacgctaa ttgttacaa cggtgaaga tttcgacgt ccccatctc aatgacacc gtgcggttg cgatattta cgactacga gtgtgattt tggtttcat ccgttcgga aaaacccgg caattgcgt ccgcaacac ttcaatcac cagcgatcc ttaccgtat tcccgccac ttgcaaacc gggcaaatt ttacaaacg gacgaaagg ctaccgaaa aatccaagg cgatgtttg cgtcgacgg ctttgagaa tcattacat gccggagga aatcccctg tccgtttaa cgaagttga cgatttcct cttgtttgc gcaacggga gagaatcag tcggtttaa tctcgtgac aaggaaact cgaagagct tccggttaa gaatttgaa aaatgtgat cgcgattga tttcgatat gcggaataa ttgcgtgta ttgggccga tattgcatt ggatacgat cggacggca gtgtttgaa cgacagtga gccggagat tttggtttc gagtgattt ggcctcgat tgaaggcat ggctttgga ttggatttc caatacttt gtattttgt cgatgggat tcggtcaaa gattgagtt gatcaggac tgatgttaa tcacagtgg gagaatgcg tagaactgt gttaaaagg cgacgtttt gaagaaacc acgagggat tgccttgca ccccaaagc tgggtacat gttctggac tgactggtc ggtggaacg gccttcggt caaccgagc tgacttgga cggttccaa tgtggttaa actgttcgg caaacctcg ggtcgaatg gcccaatgg tataaccat cgactacat cgccgaacg catctactg ggtggacgc ccgtcaaga ctacatcgc ctccagtga cttgcacgg cgatttcct caagataat aatctcgca aaacgacgt tgtctcaca cccgtttgc cgtcgcagt tttcaaaaa cacaatgta ttgggacga ttggaaacg taacgcgat ttttagcgc cgataagga cacctatca cggcgtgga aatcctcca gaagcaatt gccaggtct catggacct taaagtcta cgcgcacgg cctccaagt gggcacgaa cgcctgcac tgcctccaa atgctcata catttgtgt cagcaaacc gaaaaagaa ctcgtcttg tttatgccc cgatggcat ggaatacaa caacggacg gtgtctctg cccggggca tgtgccccc attggctaa cttcacctg tccgccggt gcaaaacac ctgcagttc gaatcattt cacgtgtcg gaacggtct atgtgtgcc caaaggctg gaagtgtga tggtgaaga tgattgtgg cgattcctc cgatgaggc gcactgtgg catgcagag ttgcccacc gaattattt cgtttgtgg tgatggcaa gtgtttgcc acagtattg gaagtgtga ctatgacta tgattgttc ggacgggtc ggatgaagt ggattgtcc gaaacagaa ctgcaccga tgggcaatt tacgtgtaa gaatggccg gtgtattgc agagaagtg gaagtgtga tggggaaaa tgattgtcg ggatgggtc agatgagtt gaattgtga accggtgga gccacacgt gtgtaaaag tgaggagtt ccagtgcaa ggttggtgg tgttaaatg tataccatc gacgtggca atgcgacgg tgaaaagga ttgtcgaga tgggtcaga tgaaatgaa ttgcaaaaa taatacgtg tctcgatat tcaattttc gtgtggtga acccaacaa tcgttgtat ttacaaaac atgggtgtg tgacggtga taaagactg tcccaatgg cagtgacga agtcaattg tacgactcc tgcaatccc tgatacaag cgatgccac tgatcagtt tatacccaa aaacgggac gtgtcagga ttggatgtt ccgttgcca gaaccagcg ctgcatccc ctattggtg gaagtgcga tcaagccga cgattgcgg cgacggcag cgatgaact tggttgttt cccaagcca tgccccgac gcccaagtc cacaaccac accccctgt tacgacttg tggtaacaa ccaattcga gtgtatggg cgggacttg tatcttttc gtcgtgggt gtgcgacgg tatggagga ttgtgtggg cggtgaaga tgaacgaca ttgtgaagg tgtacgtaa ttgcaccaa agacgagtt caaatgtca cgtggatgg aagttgtat accgttggc agcggtttg taatcgaca gccggattg tcccgattt gacagatga gtctttgtg tgatcataa tctaccgaa cggaccagc cacgccctc gtgttcaaa agggtactt cccttgtga tggaggtag ttgttaccc attggctgc attgtgcga tgggcgtgt tgattgtcg cgacggtta cgacgaatt aaattgcac gaaaacatc aagggtgta tcaagtgtt acgaatggg cgtggacga acggagtat taacgagac gagtctttt gctctactg gtggatcac agacccgcc agtcggtaa attagagtt cttaccgtc gattagtaa agtgggaga gaatgtttg ggaaaacaa aacgtggat gaaggacat ggagtataa atttaccaa tttgcaacc ggcgactca atacaatat gacgattta tgtcagggt tcaaaatac gagcaaagt gtttttacc tgcgaaata tttcgtggc ttttacggc tgaaagcat tccaagtga gccttggaa cgtgacagc tgttcaaac gaacggttc tcatgttct tttgtcttg gaataaacc aacgcatcc caacggcca aatcgtcaa ttacgagat atgttggta cccaccgaa cccaccaat caaattgaa gctgaacgg taacgaaac cgcccattt gctctccgc ctacttcga gcccgataa atattacaa cttttatgt aatcgccca caatcacaa gtatgaaag taagcgctc aaaagtcac gactttgaa attcgatgg cgacgctaa actccacgc tgtcgaaaa tttaagagt ggacaaaaa atccgaaac tagcgtcac actatcgtg gaaatataa caaaacagt tgaaggttt cgccgttgc gataattcc acccaatga ctatccgcg aatgttacc acgtgtcac caacacaca atccatcac tgtcgataa gttagcgcc cggtacccg ctacacgtt caaggttaa cgcttacaa aaatgcatt caccggccc cgaagttga cataatcgt ggtaactga cggcgtcat gttaccaga agtgccgtc acttcaagg aaaattggt caaagaagt cggaacttc ggtcaaatt gagttgggg agttccgaa agattcgag aaaagtcaa gtggacgta tggtgttta ttatggaat tgacgccga tcagttata tgaaaaatc gagatttga aacaactga tttgacgac aaccatcaa cgatttaaa agcttgcga gtattatac tttcatggt cggagttgt gggaccgta tggtcaagg cccactatc aaagagctt acaaatcag tacgtttac gaataagaa ggcaccgcc gacaaatct gaacgtcaa ggaagtgga acacaatcc gttgaaaat gatgatcac gtgggtgcc ttcgtgtcc gaccatcgc cgaacccat cggccatat gatccgtgt ctatgaaag aacaaccgg taaaatatc ctcgtatgg caaaccacc tgggaatta cagtcacgt cttgaatgt aaaatacgg cgctatcta tgacataag cgtggcaac acgcgaagt ggacgccat ctacacacc caacgtgac ttattacgc cccgcccat tttgcctcc acacgaagt gaaaacaac cattgaaca gaacggtag ttttgttgt cgattggca agaagccga cgttcccaa ggccgtagg gccgtttaa atacgaagt tttggtttc tgaaggaaa tactctgga cgaggcaac ggcgaggcg ttttcgagt ggacaaacc accatttat ctacactaa cagttcatc gaatatgta cactttcgc ggtccaaat cgtggctga tagtgggta caaatcgat cacatcgga gaagttgag tgcaacggt tttggaact gcccacgac catgggtgg taccaatct ctggttgat tttggtgct cacgatttt gtcaatagt cgtggtggt aacactggg tgtgtttat agtgaaaca tcgccgctt acagaatag tttcacgcg atttgcaaa ttcgcatta tgatacgcg gtcaggggc ggcgacttt tgacgataa tggattaga ggaggagga aagtccgca aataacagg gttttcgga tgacgagcc gctcgttat cgcgtagct gaatttgta aagtatttg atgagactt gggtttgtg gggctgtgg catggttac caaatattg aacgccacg accgtgcaa atgatttta tagtatttg tccattggt gaaaataaa gacattttt aatcatgtt gtgactatt ccaaattta ttattatat aactagtct tgtaaagag agtaatggg ctaatcact ttttctact ttgtgaaag tttgttgtt tttgtacat ttaggttta ggtttttta agtttacga ttcctacgt gtgagtgtg gtttattga gcacacgtt aatgttgcc actgattag agttgagtt acatttaat taactttaa gtcaaattt ctgtgatac ttctcagtt aagttttaa gattttttc ataaaggaa tttgactgt catatgttt gtgctttta agtgtttaa ataaatcat ttaaaatgg t
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