Status
Please wait ...
Gene information
General information
TC007849 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1403001..1411907
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007849
    iBeetle screen iB_01272

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    remarks: later than schedule, entire repetition development delayed, hours 60
    Pupal morphology
    remarks: later than schedule, entire repetition development delayed, hours 60
    Adult morphology
    remarks: later than schedule, entire repetition development delayed, hours 60
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007849 RA
      MNCLLVLLL LCHYFVENG TAEHEKYRR TIHGYEDPP 
      SNGKKTFII ERNFETGGH NVEKRSIVS TDDSKIIQK 
      INYLNDTHK QLMVHWVGE GSNVIICLA RHSSLETLA 
      GDVKVGPSA VYISYDYGD TYVNKTDLF KLSDGSYAT 
      LEKFYNHPK YNTHFVFAD VNHKVLYVT ANHGRDIKR 
      IDLDFTPSD VSFHELQPS VFVVLDKVD HLHKLWVTE 
      DFGNSFRAT QDFVKATYW IKENDLRHT LLIQRMEPS 
      GYNAILSSS DLFSNRTVM VQATGIKEF FVKGDYLFT 
      TKLSSKGVL ELYVSYKLG KKIKCVFDT TLEIRSYFI 
      VDVTSNRAL VAVSHADTV SHLYVSENL DNSEGVVKF 
      TLSLEDVLC YFPNSTWHD TWIHHVSEE AFADVYKVE 
      GLSGIYIAS RVLTKPQGN NLGPQHLGS VITFDHGRS 
      WRLIQAPFR DVEGQPTSC SVQQNCSLH LSQKFSQLY 
      PDTRSISIL SSKSAPGLL IATGVLGKS LKGHYGVYI 
      SSDAGQTWR QTLRDLYFF NMGDHGGIL TAVKYFKLR 
      GETRHVLYS TDEGETWKQ AAFHDQNIR LYGLMTEPG 
      ENTTVFTMF GSLPEEHQW IIIKLDLIK AFAYNCSKD 
      DYKPWSPSQ SDENRSYIP CVLGQQVTY HRRMPHANC 
      YNGEDFDVP ISMTPCGCD IYDYECDFG FIRSEKPGN 
      CVRNTSITS DPYRIPATC KPGKFYKRT KGYRKIQGD 
      VCVDGFENH YMPEEIPCP FNEVDDFLL FAQRERISR 
      FNLVTRKLE ELPVKNLKN VIAIDFDMR NNCVYWADI 
      ALDTIGRQC LNDSEPEIL VSSDLASIE GMALDWISN 
      TLYFVDGIR SKIELIRTD VNHSGRMRR TVLKGDVLK 
      KPRGIALHP KAGYMFWTD WSVERPSVN RADLDGSNV 
      VKLFGKPRV EWPNGITID YIAERIYWV DARQDYIAS 
      SDLHGDFLK IIISQNDVV SHPFAVAVF KNTMYWDDW 
      KRNAIFSAD KDTYHGVEI LQKQLPGLM DLKVYAHGL 
      QVGTNACTA SKCSYICVS KPKKNSSCL CPDGMEYNN 
      GRCLCPGHV PPLANFTCP PVQNTCSSN HFTCRNGLC 
      VPKGWKCDG EDDCGDSSD EAHCGMQSC PPNYFVCGD 
      GKCLPQYWK CDYDYDCSD GSDEVDCPK QNCTDGQFT 
      CKNGRCIAE KWKCDGEND CRDGSDELN CEPVEPHVC 
      KSEEFQCKV GGVKCIPST WQCDGEKDC RDGSDEMNC 
      KNNTCLDIQ FSCGEPNNR CIYKTWVCD GDKDCPNGS 
      DEVNCTTPA IPDTSDATD QFIPKNGTC QDWMFRCQN 
      QRCIPYWWK CDQADDCGD GSDELGCFP SHAPTPKST 
      TTPPVTTCG NNQFECMGG TCIFSSWVC DGMEDCVGG 
      EDERHCEGV RNCTKDEFK CHVDGSCIP LAAVCNRQP 
      DCPDLTDES LCDHNLPNG PATPSCSKG YFPCDGGSC 
      YPLAALCDG RVDCRDGYD ELNCTKTSR VYQVLRMGV 
      DERSINETS LLLYWWITD PPVGKLEFL PSISKVGEN 
      VWENKTWMK DMEYKFTNL QPATQYNMT IYVRVQNTS 
      KVFLPAKYF VAFTAESIP SEPWNVTAV QTNGSHVLL 
      SWNKPTHPN GQIVNYEIC WYPPNPPIK LKLNGNETA 
      HLLSAYFEP DKYYNFYVI AHNHKYESK RSKVTTLKF 
      DGDAKLHAV ENLRVDKKS ETSVTLSWK YNKTVEGFA 
      VAIIPPNDY PRMLPRVTN TQSITVDKL APGTRYTFK 
      VNAYKNAFT GPEVDIIVV TDGVMLPEV PSLQGKLVK 
      EVGTSVKLS WGVPKDSRK VKWTYGVYY GIDADQLYE 
      KSRFETTDL TTTINDLKA CEYYTFMVG VVGPYGQGP 
      LSKSLQIST FTNKKAPPT NLNVKEVEH NPLKMMITW 
      VPSCPTIAE PIGHMIRVY ERTTGKISS YGKPPGNYS 
      HVLNVKYGA IYDISVATR EVDAIYTPN VTYYAPPIL 
      PPHEVKTTI EQNGSFVVD WQEADVPKA VGPFKYEVL 
      VSEGNTLDE ATARRFRVD KPPFIYTNS SSNMYTFAV 
      QIVADSGYK SITSEKLSA TVLELPTTM GGTNLWLIL 
      VLTILSIVV VVTLGVFIV KHRRLQNSF TRFANSHYD 
      TRSGAATFD DNGLEEEES PQITGFSDD EPLVIA
    CDS sequence
    • >TC007849 RA
      atgaactgt ctccttgtt ttgttattg ttgtgccat 
      tatttcgtg gaaaatggc acagccgaa cacgaaaag 
      tacagacga acgatacat gggtacgag gatccgcca 
      agcaatggc aaaaaaacc ttcattatt gagcgaaat 
      ttcgaaacc gggggacac aacgtcgaa aaaagatct 
      atcgtgtcc actgatgac agtaaaatc atacagaag 
      ataaattat ctgaatgac acacacaaa caactgatg 
      gtgcattgg gtgggcgaa ggtagcaac gttattatt 
      tgcctagca cgtcactcg tctcttgaa acattggct 
      ggggatgta aaagttgga ccatcggcc gtttatatt 
      tcctacgat tatggcgac acttatgtc aacaaaact 
      gatcttttt aaattatcg gacgggtct tatgccact 
      ttggaaaag ttttacaac catccgaaa tacaacaca 
      cattttgtt tttgccgac gtaaaccat aaagtactc 
      tacgtaacg gcgaatcac ggccgtgac ataaaacgc 
      atcgacttg gatttcaca ccgagcgac gtttcgttt 
      cacgaactg caaccgtca gtgtttgtc gttttggat 
      aaagtcgat catctgcat aaattatgg gtgactgaa 
      gatttcggt aatagtttt cgagcgaca caggatttc 
      gtcaaagcc acttattgg atcaaagag aatgatttg 
      agacacact ttattgata caaaggatg gaaccgagt 
      ggttataac gcgatttta tcatcgagc gatttattc 
      tcgaatcga acggttatg gtccaagcc accggtatt 
      aaggagttc ttcgtcaaa ggcgattat ctcttcacg 
      accaaatta tcgagcaaa ggagtgctc gagttgtac 
      gtttcgtat aaacttggg aagaaaatt aagtgtgtt 
      ttcgatacg actttagag attcggagc tatttcatt 
      gttgatgtt acttcgaat cgggctctt gtggcagtg 
      agtcatgcc gatactgtc tcacatttg tacgtttcg 
      gagaatttg gacaacagt gagggagtt gttaaattc 
      acactgtca ctagaagac gtcctttgc tattttccg 
      aacagtaca tggcacgat acttggatc caccacgtc 
      tcagaggaa gctttcgcc gatgtttac aaagtcgaa 
      ggtctctca ggcatttac atcgcctca cgtgtctta 
      accaaaccc cagggtaac aatctcggt ccgcaacac 
      ttaggttcg gttataacc ttcgaccac ggccgatct 
      tggcgtctg atccaagcc cctttccgt gatgtcgaa 
      ggacaaccg acttcctgc tccgtccaa cagaattgc 
      tccctccat ttgagccaa aagttcagt caattgtac 
      cccgatact agatctatt agtattcta tcgagtaaa 
      tcggcgcct gggctttta atcgcaaca ggtgttttg 
      ggaaaatca cttaaagga cattacggt gtttatata 
      agtagtgat gcgggacag acgtggaga cagactttg 
      agggatttg tatttcttt aatatgggt gaccatggt 
      gggatttta acagcggtt aagtatttt aaattgagg 
      ggggagacg aggcatgtt ttgtattca acggacgag 
      ggagagaca tggaaacag gcggctttt cacgatcag 
      aatattcgc ttgtatggg cttatgacc gaaccgggg 
      gaaaacacg acagtgttt acgatgttt ggctccttg 
      ccggaggaa catcagtgg attattatc aagctggat 
      ttaataaag gccttcgcc tataactgc tcaaaagac 
      gactacaag ccgtggtcc ccgagccaa agcgacgaa 
      aaccgcagt tacatccct tgcgttttg ggccaacaa 
      gtcacctac caccgtcgc atgccccac gctaattgt 
      tacaacggt gaagatttc gacgtcccc atctcaatg 
      acaccgtgc ggttgcgat atttacgac tacgagtgt 
      gattttggt ttcatccgt tcggaaaaa cccggcaat 
      tgcgtccgc aacacttca atcaccagc gatccttac 
      cgtattccc gccacttgc aaaccgggc aaattttac 
      aaacggacg aaaggctac cgaaaaatc caaggcgat 
      gtttgcgtc gacggcttt gagaatcat tacatgccg 
      gaggaaatc ccctgtccg tttaacgaa gttgacgat 
      ttcctcttg tttgcgcaa cgggagaga atcagtcgg 
      tttaatctc gtgacaagg aaactcgaa gagcttccg 
      gttaagaat ttgaaaaat gtgatcgcg attgatttc 
      gatatgcgg aataattgc gtgtattgg gccgatatt 
      gcattggat acgatcgga cggcagtgt ttgaacgac 
      agtgagccg gagattttg gtttcgagt gatttggcc 
      tcgattgaa ggcatggct ttggattgg atttccaat 
      actttgtat tttgtcgat gggattcgg tcaaagatt 
      gagttgatc aggactgat gttaatcac agtgggaga 
      atgcgtaga actgtgtta aaaggcgac gttttgaag 
      aaaccacga gggattgcc ttgcacccc aaagctggg 
      tacatgttc tggactgac tggtcggtg gaacggcct 
      tcggtcaac cgagctgac ttggacggt tccaatgtg 
      gttaaactg ttcggcaaa cctcgggtc gaatggccc 
      aatggtata accatcgac tacatcgcc gaacgcatc 
      tactgggtg gacgcccgt caagactac atcgcctcc 
      agtgacttg cacggcgat ttcctcaag ataataatc 
      tcgcaaaac gacgttgtc tcacacccg tttgccgtc 
      gcagttttc aaaaacaca atgtattgg gacgattgg 
      aaacgtaac gcgattttt agcgccgat aaggacacc 
      tatcacggc gtggaaatc ctccagaag caattgcca 
      ggtctcatg gaccttaaa gtctacgcg cacggcctc 
      caagtgggc acgaacgcc tgcactgcc tccaaatgc 
      tcatacatt tgtgtcagc aaaccgaaa aagaactcg 
      tcttgttta tgccccgat ggcatggaa tacaacaac 
      ggacggtgt ctctgcccg gggcatgtg cccccattg 
      gctaacttc acctgtccg ccggtgcaa aacacctgc 
      agttcgaat catttcacg tgtcggaac ggtctatgt 
      gtgcccaaa ggctggaag tgtgatggt gaagatgat 
      tgtggcgat tcctccgat gaggcgcac tgtggcatg 
      cagagttgc ccaccgaat tatttcgtt tgtggtgat 
      ggcaagtgt ttgccacag tattggaag tgtgactat 
      gactatgat tgttcggac gggtcggat gaagtggat 
      tgtccgaaa cagaactgc accgatggg caatttacg 
      tgtaagaat ggccggtgt attgcagag aagtggaag 
      tgtgatggg gaaaatgat tgtcgggat gggtcagat 
      gagttgaat tgtgaaccg gtggagcca cacgtgtgt 
      aaaagtgag gagttccag tgcaaggtt ggtggtgtt 
      aaatgtata ccatcgacg tggcaatgc gacggtgaa 
      aaggattgt cgagatggg tcagatgaa atgaattgc 
      aaaaataat acgtgtctc gatattcaa ttttcgtgt 
      ggtgaaccc aacaatcgt tgtatttac aaaacatgg 
      gtgtgtgac ggtgataaa gactgtccc aatggcagt 
      gacgaagtc aattgtacg actcctgca atccctgat 
      acaagcgat gccactgat cagtttata cccaaaaac 
      gggacgtgt caggattgg atgttccgt tgccagaac 
      cagcgctgc atcccctat tggtggaag tgcgatcaa 
      gccgacgat tgcggcgac ggcagcgat gaacttggt 
      tgtttccca agccatgcc ccgacgccc aagtccaca 
      accacaccc cctgttacg acttgtggt aacaaccaa 
      ttcgagtgt atgggcggg acttgtatc ttttcgtcg 
      tgggtgtgc gacggtatg gaggattgt gtgggcggt 
      gaagatgaa cgacattgt gaaggtgta cgtaattgc 
      accaaagac gagttcaaa tgtcacgtg gatggaagt 
      tgtataccg ttggcagcg gtttgtaat cgacagccg 
      gattgtccc gatttgaca gatgagtct ttgtgtgat 
      cataatcta ccgaacgga ccagccacg ccctcgtgt 
      tcaaaaggg tacttccct tgtgatgga ggtagttgt 
      tacccattg gctgcattg tgcgatggg cgtgttgat 
      tgtcgcgac ggttacgac gaattaaat tgcacgaaa 
      acatcaagg gtgtatcaa gtgttacga atgggcgtg 
      gacgaacgg agtattaac gagacgagt cttttgctc 
      tactggtgg atcacagac ccgccagtc ggtaaatta 
      gagttctta ccgtcgatt agtaaagtg ggagagaat 
      gtttgggaa aacaaaacg tggatgaag gacatggag 
      tataaattt accaatttg caaccggcg actcaatac 
      aatatgacg atttatgtc agggttcaa aatacgagc 
      aaagtgttt ttacctgcg aaatatttc gtggctttt 
      acggctgaa agcattcca agtgagcct tggaacgtg 
      acagctgtt caaacgaac ggttctcat gttcttttg 
      tcttggaat aaaccaacg catcccaac ggccaaatc 
      gtcaattac gagatatgt tggtaccca ccgaaccca 
      ccaatcaaa ttgaagctg aacggtaac gaaaccgcc 
      catttgctc tccgcctac ttcgagccc gataaatat 
      tacaacttt tatgtaatc gcccacaat cacaagtat 
      gaaagtaag cgctcaaaa gtcacgact ttgaaattc 
      gatggcgac gctaaactc cacgctgtc gaaaattta 
      agagtggac aaaaaatcc gaaactagc gtcacacta 
      tcgtggaaa tataacaaa acagttgaa ggtttcgcc 
      gttgcgata attccaccc aatgactat ccgcgaatg 
      ttaccacgt gtcaccaac acacaatcc atcactgtc 
      gataagtta gcgcccggt acccgctac acgttcaag 
      gttaacgct tacaaaaat gcattcacc ggccccgaa 
      gttgacata atcgtggta actgacggc gtcatgtta 
      ccagaagtg ccgtcactt caaggaaaa ttggtcaaa 
      gaagtcgga acttcggtc aaattgagt tggggagtt 
      ccgaaagat tcgagaaaa gtcaagtgg acgtatggt 
      gtttattat ggaattgac gccgatcag ttatatgaa 
      aaatcgaga tttgaaaca actgatttg acgacaacc 
      atcaacgat ttaaaagct tgcgagtat tatactttc 
      atggtcgga gttgtggga ccgtatggt caaggccca 
      ctatcaaag agcttacaa atcagtacg tttacgaat 
      aagaaggca ccgccgaca aatctgaac gtcaaggaa 
      gtggaacac aatccgttg aaaatgatg atcacgtgg 
      gtgccttcg tgtccgacc atcgccgaa cccatcggc 
      catatgatc cgtgtctat gaaagaaca accggtaaa 
      atatcctcg tatggcaaa ccacctggg aattacagt 
      cacgtcttg aatgtaaaa tacggcgct atctatgac 
      ataagcgtg gcaacacgc gaagtggac gccatctac 
      acacccaac gtgacttat tacgccccg cccattttg 
      cctccacac gaagtgaaa acaaccatt gaacagaac 
      ggtagtttt gttgtcgat tggcaagaa gccgacgtt 
      cccaaggcc gtagggccg tttaaatac gaagttttg 
      gtttctgaa ggaaatact ctggacgag gcaacggcg 
      aggcgtttt cgagtggac aaaccacca tttatctac 
      actaacagt tcatcgaat atgtacact ttcgcggtc 
      caaatcgtg gctgatagt gggtacaaa tcgatcaca 
      tcggagaag ttgagtgca acggttttg gaactgccc 
      acgaccatg ggtggtacc aatctctgg ttgattttg 
      gtgctcacg attttgtca atagtcgtg gtggtaaca 
      ctgggtgtg tttatagtg aaacatcgc cgcttacag 
      aatagtttc acgcgattt gcaaattcg cattatgat 
      acgcggtca ggggcggcg acttttgac gataatgga 
      ttagaggag gaggaaagt ccgcaaata acagggttt 
      tcggatgac gagccgctc gttatcgcg tag
    mRNA sequence
    • >TC007849 RA
      tttttttga cacatgacg cacttcttt cagtcatca 
      gctgacagt tgttgtgaa tgggtgctc aaaaatgaa 
      ctgtctcct tgttttgtt attgttgtg ccattattt 
      cgtggaaaa tggcacagc cgaacacga aaagtacag 
      acgaacgat acatgggta cgaggatcc gccaagcaa 
      tggcaaaaa aaccttcat tattgagcg aaatttcga 
      aaccggggg acacaacgt cgaaaaaag atctatcgt 
      gtccactga tgacagtaa aatcataca gaagataaa 
      ttatctgaa tgacacaca caaacaact gatggtgca 
      ttgggtggg cgaaggtag caacgttat tatttgcct 
      agcacgtca ctcgtctct tgaaacatt ggctgggga 
      tgtaaaagt tggaccatc ggccgttta tatttccta 
      cgattatgg cgacactta tgtcaacaa aactgatct 
      ttttaaatt atcggacgg gtcttatgc cactttgga 
      aaagtttta caaccatcc gaaatacaa cacacattt 
      tgtttttgc cgacgtaaa ccataaagt actctacgt 
      aacggcgaa tcacggccg tgacataaa acgcatcga 
      cttggattt cacaccgag cgacgtttc gtttcacga 
      actgcaacc gtcagtgtt tgtcgtttt ggataaagt 
      cgatcatct gcataaatt atgggtgac tgaagattt 
      cggtaatag ttttcgagc gacacagga tttcgtcaa 
      agccactta ttggatcaa agagaatga tttgagaca 
      cactttatt gatacaaag gatggaacc gagtggtta 
      taacgcgat tttatcatc gagcgattt attctcgaa 
      tcgaacggt tatggtcca agccaccgg tattaagga 
      gttcttcgt caaaggcga ttatctctt cacgaccaa 
      attatcgag caaaggagt gctcgagtt gtacgtttc 
      gtataaact tgggaagaa aattaagtg tgttttcga 
      tacgacttt agagattcg gagctattt cattgttga 
      tgttacttc gaatcgggc tcttgtggc agtgagtca 
      tgccgatac tgtctcaca tttgtacgt ttcggagaa 
      tttggacaa cagtgaggg agttgttaa attcacact 
      gtcactaga agacgtcct ttgctattt tccgaacag 
      tacatggca cgatacttg gatccacca cgtctcaga 
      ggaagcttt cgccgatgt ttacaaagt cgaaggtct 
      ctcaggcat ttacatcgc ctcacgtgt cttaaccaa 
      accccaggg taacaatct cggtccgca acacttagg 
      ttcggttat aaccttcga ccacggccg atcttggcg 
      tctgatcca agccccttt ccgtgatgt cgaaggaca 
      accgacttc ctgctccgt ccaacagaa ttgctccct 
      ccatttgag ccaaaagtt cagtcaatt gtaccccga 
      tactagatc tattagtat tctatcgag taaatcggc 
      gcctgggct tttaatcgc aacaggtgt tttgggaaa 
      atcacttaa aggacatta cggtgttta tataagtag 
      tgatgcggg acagacgtg gagacagac tttgaggga 
      tttgtattt ctttaatat gggtgacca tggtgggat 
      tttaacagc ggttaagta ttttaaatt gagggggga 
      gacgaggca tgttttgta ttcaacgga cgagggaga 
      gacatggaa acaggcggc ttttcacga tcagaatat 
      tcgcttgta tgggcttat gaccgaacc gggggaaaa 
      cacgacagt gtttacgat gtttggctc cttgccgga 
      ggaacatca gtggattat tatcaagct ggatttaat 
      aaaggcctt cgcctataa ctgctcaaa agacgacta 
      caagccgtg gtccccgag ccaaagcga cgaaaaccg 
      cagttacat cccttgcgt tttgggcca acaagtcac 
      ctaccaccg tcgcatgcc ccacgctaa ttgttacaa 
      cggtgaaga tttcgacgt ccccatctc aatgacacc 
      gtgcggttg cgatattta cgactacga gtgtgattt 
      tggtttcat ccgttcgga aaaacccgg caattgcgt 
      ccgcaacac ttcaatcac cagcgatcc ttaccgtat 
      tcccgccac ttgcaaacc gggcaaatt ttacaaacg 
      gacgaaagg ctaccgaaa aatccaagg cgatgtttg 
      cgtcgacgg ctttgagaa tcattacat gccggagga 
      aatcccctg tccgtttaa cgaagttga cgatttcct 
      cttgtttgc gcaacggga gagaatcag tcggtttaa 
      tctcgtgac aaggaaact cgaagagct tccggttaa 
      gaatttgaa aaatgtgat cgcgattga tttcgatat 
      gcggaataa ttgcgtgta ttgggccga tattgcatt 
      ggatacgat cggacggca gtgtttgaa cgacagtga 
      gccggagat tttggtttc gagtgattt ggcctcgat 
      tgaaggcat ggctttgga ttggatttc caatacttt 
      gtattttgt cgatgggat tcggtcaaa gattgagtt 
      gatcaggac tgatgttaa tcacagtgg gagaatgcg 
      tagaactgt gttaaaagg cgacgtttt gaagaaacc 
      acgagggat tgccttgca ccccaaagc tgggtacat 
      gttctggac tgactggtc ggtggaacg gccttcggt 
      caaccgagc tgacttgga cggttccaa tgtggttaa 
      actgttcgg caaacctcg ggtcgaatg gcccaatgg 
      tataaccat cgactacat cgccgaacg catctactg 
      ggtggacgc ccgtcaaga ctacatcgc ctccagtga 
      cttgcacgg cgatttcct caagataat aatctcgca 
      aaacgacgt tgtctcaca cccgtttgc cgtcgcagt 
      tttcaaaaa cacaatgta ttgggacga ttggaaacg 
      taacgcgat ttttagcgc cgataagga cacctatca 
      cggcgtgga aatcctcca gaagcaatt gccaggtct 
      catggacct taaagtcta cgcgcacgg cctccaagt 
      gggcacgaa cgcctgcac tgcctccaa atgctcata 
      catttgtgt cagcaaacc gaaaaagaa ctcgtcttg 
      tttatgccc cgatggcat ggaatacaa caacggacg 
      gtgtctctg cccggggca tgtgccccc attggctaa 
      cttcacctg tccgccggt gcaaaacac ctgcagttc 
      gaatcattt cacgtgtcg gaacggtct atgtgtgcc 
      caaaggctg gaagtgtga tggtgaaga tgattgtgg 
      cgattcctc cgatgaggc gcactgtgg catgcagag 
      ttgcccacc gaattattt cgtttgtgg tgatggcaa 
      gtgtttgcc acagtattg gaagtgtga ctatgacta 
      tgattgttc ggacgggtc ggatgaagt ggattgtcc 
      gaaacagaa ctgcaccga tgggcaatt tacgtgtaa 
      gaatggccg gtgtattgc agagaagtg gaagtgtga 
      tggggaaaa tgattgtcg ggatgggtc agatgagtt 
      gaattgtga accggtgga gccacacgt gtgtaaaag 
      tgaggagtt ccagtgcaa ggttggtgg tgttaaatg 
      tataccatc gacgtggca atgcgacgg tgaaaagga 
      ttgtcgaga tgggtcaga tgaaatgaa ttgcaaaaa 
      taatacgtg tctcgatat tcaattttc gtgtggtga 
      acccaacaa tcgttgtat ttacaaaac atgggtgtg 
      tgacggtga taaagactg tcccaatgg cagtgacga 
      agtcaattg tacgactcc tgcaatccc tgatacaag 
      cgatgccac tgatcagtt tatacccaa aaacgggac 
      gtgtcagga ttggatgtt ccgttgcca gaaccagcg 
      ctgcatccc ctattggtg gaagtgcga tcaagccga 
      cgattgcgg cgacggcag cgatgaact tggttgttt 
      cccaagcca tgccccgac gcccaagtc cacaaccac 
      accccctgt tacgacttg tggtaacaa ccaattcga 
      gtgtatggg cgggacttg tatcttttc gtcgtgggt 
      gtgcgacgg tatggagga ttgtgtggg cggtgaaga 
      tgaacgaca ttgtgaagg tgtacgtaa ttgcaccaa 
      agacgagtt caaatgtca cgtggatgg aagttgtat 
      accgttggc agcggtttg taatcgaca gccggattg 
      tcccgattt gacagatga gtctttgtg tgatcataa 
      tctaccgaa cggaccagc cacgccctc gtgttcaaa 
      agggtactt cccttgtga tggaggtag ttgttaccc 
      attggctgc attgtgcga tgggcgtgt tgattgtcg 
      cgacggtta cgacgaatt aaattgcac gaaaacatc 
      aagggtgta tcaagtgtt acgaatggg cgtggacga 
      acggagtat taacgagac gagtctttt gctctactg 
      gtggatcac agacccgcc agtcggtaa attagagtt 
      cttaccgtc gattagtaa agtgggaga gaatgtttg 
      ggaaaacaa aacgtggat gaaggacat ggagtataa 
      atttaccaa tttgcaacc ggcgactca atacaatat 
      gacgattta tgtcagggt tcaaaatac gagcaaagt 
      gtttttacc tgcgaaata tttcgtggc ttttacggc 
      tgaaagcat tccaagtga gccttggaa cgtgacagc 
      tgttcaaac gaacggttc tcatgttct tttgtcttg 
      gaataaacc aacgcatcc caacggcca aatcgtcaa 
      ttacgagat atgttggta cccaccgaa cccaccaat 
      caaattgaa gctgaacgg taacgaaac cgcccattt 
      gctctccgc ctacttcga gcccgataa atattacaa 
      cttttatgt aatcgccca caatcacaa gtatgaaag 
      taagcgctc aaaagtcac gactttgaa attcgatgg 
      cgacgctaa actccacgc tgtcgaaaa tttaagagt 
      ggacaaaaa atccgaaac tagcgtcac actatcgtg 
      gaaatataa caaaacagt tgaaggttt cgccgttgc 
      gataattcc acccaatga ctatccgcg aatgttacc 
      acgtgtcac caacacaca atccatcac tgtcgataa 
      gttagcgcc cggtacccg ctacacgtt caaggttaa 
      cgcttacaa aaatgcatt caccggccc cgaagttga 
      cataatcgt ggtaactga cggcgtcat gttaccaga 
      agtgccgtc acttcaagg aaaattggt caaagaagt 
      cggaacttc ggtcaaatt gagttgggg agttccgaa 
      agattcgag aaaagtcaa gtggacgta tggtgttta 
      ttatggaat tgacgccga tcagttata tgaaaaatc 
      gagatttga aacaactga tttgacgac aaccatcaa 
      cgatttaaa agcttgcga gtattatac tttcatggt 
      cggagttgt gggaccgta tggtcaagg cccactatc 
      aaagagctt acaaatcag tacgtttac gaataagaa 
      ggcaccgcc gacaaatct gaacgtcaa ggaagtgga 
      acacaatcc gttgaaaat gatgatcac gtgggtgcc 
      ttcgtgtcc gaccatcgc cgaacccat cggccatat 
      gatccgtgt ctatgaaag aacaaccgg taaaatatc 
      ctcgtatgg caaaccacc tgggaatta cagtcacgt 
      cttgaatgt aaaatacgg cgctatcta tgacataag 
      cgtggcaac acgcgaagt ggacgccat ctacacacc 
      caacgtgac ttattacgc cccgcccat tttgcctcc 
      acacgaagt gaaaacaac cattgaaca gaacggtag 
      ttttgttgt cgattggca agaagccga cgttcccaa 
      ggccgtagg gccgtttaa atacgaagt tttggtttc 
      tgaaggaaa tactctgga cgaggcaac ggcgaggcg 
      ttttcgagt ggacaaacc accatttat ctacactaa 
      cagttcatc gaatatgta cactttcgc ggtccaaat 
      cgtggctga tagtgggta caaatcgat cacatcgga 
      gaagttgag tgcaacggt tttggaact gcccacgac 
      catgggtgg taccaatct ctggttgat tttggtgct 
      cacgatttt gtcaatagt cgtggtggt aacactggg 
      tgtgtttat agtgaaaca tcgccgctt acagaatag 
      tttcacgcg atttgcaaa ttcgcatta tgatacgcg 
      gtcaggggc ggcgacttt tgacgataa tggattaga 
      ggaggagga aagtccgca aataacagg gttttcgga 
      tgacgagcc gctcgttat cgcgtagct gaatttgta 
      aagtatttg atgagactt gggtttgtg gggctgtgg 
      catggttac caaatattg aacgccacg accgtgcaa 
      atgatttta tagtatttg tccattggt gaaaataaa 
      gacattttt aatcatgtt gtgactatt ccaaattta 
      ttattatat aactagtct tgtaaagag agtaatggg 
      ctaatcact ttttctact ttgtgaaag tttgttgtt 
      tttgtacat ttaggttta ggtttttta agtttacga 
      ttcctacgt gtgagtgtg gtttattga gcacacgtt 
      aatgttgcc actgattag agttgagtt acatttaat 
      taactttaa gtcaaattt ctgtgatac ttctcagtt 
      aagttttaa gattttttc ataaaggaa tttgactgt 
      catatgttt gtgctttta agtgtttaa ataaatcat 
      ttaaaatgg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY