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Gene information
General information
TC007846 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 1392981..1395035
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007846
    iBeetle screen iB_01271
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007846 RA
      MDYELPTEF DIIIIGTGV IESIISAAA SRIGKRVLH 
      IDSNNYYGG LWASFNLDA IQKLATVEE TLNEGLGNT 
      FFNVKNFEI EWHIPSETP PESTEWSRQ SLLKESRRF 
      NLDLAPKLQ FARGDFVEL LISSNIARY SEYRSVSRV 
      LTWLNGQLE TVPCSRSDV FANNKVTVI EKRMLMKLF 
      LALDSGEED YHNYENKTF RAFLTDKKL TPNLIHYVL 
      YAIAMCTDD TPCLQGIKN TKRFLDSLG RFGKTPFLF 
      SMYGSGEIT QAFCRLSAV FGGIYALNQ PLKGLILNG 
      DNFEGITCG TQEIKAGVL VMGAEKAPP HFIKQHPKS 
      SIARAILIT DKSIMESEK EHLTLLLYP PEGGKNSCV 
      VLELGCLTG TCPKDLFVV HLISRQVEG PQNDFKHII 
      GNLFGPEKA NILWSCYFS IPDSNDLDL TVETPKNVT 
      LCPGPDLDL DYDDAVRKA KAMFTDIFP DCEFLPRAP 
      DPEEIIIEG ETSDQDNVE LKAESAKTE E
    CDS sequence
    • >TC007846 RA
      atggattat gaacttccg acagagttc gatattata 
      attataggg acaggcgtt atcgagtca ataatttca 
      gcagcggct agcagaatc ggaaaacga gtcttacac 
      attgatagt aacaattac tatgggggg ctttgggcc 
      tcgttcaac ttggacgca attcaaaaa ctagccacg 
      gttgaagaa acactaaat gagggtttg gggaacact 
      tttttcaat gtaaaaaat ttcgaaatt gaatggcac 
      attccgagc gaaacgccc ccagaatca accgaatgg 
      tcccgccaa agcctcctg aaagaatcg cgcagattt 
      aacctagac ctcgcccca aaactccaa ttcgcacgc 
      ggtgatttc gtcgaactt ttaatctcg tcaaacatt 
      gcccgttat tccgaatat aggagcgtt agcagagtc 
      ctgacttgg ctcaacggc caactggag acagtcccg 
      tgttcacgt tcggacgtt ttcgccaat aacaaagtc 
      acagtgatt gaaaaacgc atgcttatg aagctcttc 
      ctggcgtta gatagtggc gaagaagat tatcacaat 
      tatgaaaat aaaaccttc cgtgcgttt ttaaccgat 
      aaaaaattg accccgaat ttgattcac tacgtcctt 
      tatgcaatc gcaatgtgc actgatgac acgccctgt 
      ttgcaagga atcaaaaac acgaagcga tttttggac 
      agtttaggc cgttttggc aaaaccccc tttttattt 
      tccatgtat ggaagtggg gaaatcacc caagcgttt 
      tgtagattg agtgccgtt tttggcggt atttacgcc 
      cttaatcaa ccactgaaa ggcctaatt ttaaacggc 
      gataatttt gaaggcatc acttgtggc acacaggaa 
      atcaaagcg ggggttcta gtcatgggg gctgagaaa 
      gccccccca catttcatc aaacaacac ccgaaatca 
      tccatcgca agagctatt ttaatcaca gataaatct 
      ataatggag agtgaaaag gaacacttg actttgctc 
      ctatacccc ccagaaggg ggcaaaaat tcatgcgtg 
      gtcctagaa ctggggtgt ttgaccggg acttgcccc 
      aaagactta tttgtggta catttgatt tcacgacag 
      gttgagggg ccccaaaat gattttaaa cacattatt 
      gggaatttg tttgggcct gaaaaggca aatattttg 
      tggtcttgc tatttttca attcctgat tcaaatgat 
      ttggatttg actgtggaa acccccaaa aatgtgaca 
      ctgtgtcca gggccggac ctagacctg gattatgat 
      gatgctgtg agaaaggcc aaggctatg tttacggac 
      attttcccc gactgtgag ttcttgcca cgggccccc 
      gaccctgaa gaaatcatc attgagggc gaaacgtcg 
      gatcaagac aacgttgaa cttaaggct gagagcgca 
      aaaacggaa gaatag
    mRNA sequence
    • >TC007846 RA
      tgacagttg gattcgact ttacgaaca aacaataat 
      ttgttatca aattttaca ttattttac attgtcaca 
      aactactta gctatttat attggtcac cttttcttg 
      ttcttcgtt ttgcaaaaa aattggtgc aatacttgc 
      ccacaaaaa aataaccta aaagctgcg aatgtgatg 
      taattagca caaaaccac ctaaaccaa agaaaaaat 
      cagtatttt tgtgatgga ttatgaact tccgacaga 
      gttcgatat tataattat agggacagg cgttatcga 
      gtcaataat ttcagcagc ggctagcag aatcggaaa 
      acgagtctt acacattga tagtaacaa ttactatgg 
      ggggctttg ggcctcgtt caacttgga cgcaattca 
      aaaactagc cacggttga agaaacact aaatgaggg 
      tttggggaa cactttttt caatgtaaa aaatttcga 
      aattgaatg gcacattcc gagcgaaac gcccccaga 
      atcaaccga atggtcccg ccaaagcct cctgaaaga 
      atcgcgcag atttaacct agacctcgc cccaaaact 
      ccaattcgc acgcggtga tttcgtcga acttttaat 
      ctcgtcaaa cattgcccg ttattccga atataggag 
      cgttagcag agtcctgac ttggctcaa cggccaact 
      ggagacagt cccgtgttc acgttcgga cgttttcgc 
      caataacaa agtcacagt gattgaaaa acgcatgct 
      tatgaagct cttcctggc gttagatag tggcgaaga 
      agattatca caattatga aaataaaac cttccgtgc 
      gtttttaac cgataaaaa attgacccc gaatttgat 
      tcactacgt cctttatgc aatcgcaat gtgcactga 
      tgacacgcc ctgtttgca aggaatcaa aaacacgaa 
      gcgattttt ggacagttt aggccgttt tggcaaaac 
      ccccttttt attttccat gtatggaag tggggaaat 
      cacccaagc gttttgtag attgagtgc cgtttttgg 
      cggtattta cgcccttaa tcaaccact gaaaggcct 
      aattttaaa cggcgataa ttttgaagg catcacttg 
      tggcacaca ggaaatcaa agcgggggt tctagtcat 
      gggggctga gaaagcccc cccacattt catcaaaca 
      acacccgaa atcatccat cgcaagagc tattttaat 
      cacagataa atctataat ggagagtga aaaggaaca 
      cttgacttt gctcctata ccccccaga agggggcaa 
      aaattcatg cgtggtcct agaactggg gtgtttgac 
      cgggacttg ccccaaaga cttatttgt ggtacattt 
      gatttcacg acaggttga ggggcccca aaatgattt 
      taaacacat tattgggaa tttgtttgg gcctgaaaa 
      ggcaaatat tttgtggtc ttgctattt ttcaattcc 
      tgattcaaa tgatttgga tttgactgt ggaaacccc 
      caaaaatgt gacactgtg tccagggcc ggacctaga 
      cctggatta tgatgatgc tgtgagaaa ggccaaggc 
      tatgtttac ggacatttt ccccgactg tgagttctt 
      gccacgggc ccccgaccc tgaagaaat catcattga 
      gggcgaaac gtcggatca agacaacgt tgaacttaa 
      ggctgagag cgcaaaaac ggaagaata gatccattt 
      tatatttat tatatt