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Gene information
General information
TC007831 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1133251..1138073
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007831
    iBeetle screen iB_01270

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007831 RA
      MKLVPGPLP PIPTSTSMD RLALPLPFS PDSLLWRYP 
      LGFASIGAQ QPPSSPLLD YKSQLPSNL ASDPRVWSR 
      EDVATFLRW AEREFDLQP IDMDMFQMN GKAICLLTR 
      TDLAERAPG SGDVLYNVL QLLVRDANN LQRMLPSSP 
      VTPTSRHPY PLSPHSHPP TPNWNILAQ NSDPFHPSH 
      AASLAAHLM AQSNSVTLS PAPSVDSQA GSPQHADNP 
      QTFGHSIFS TGASNGSGS NPSDSDEDG YEKNGQTGS 
      PPNTPSAYS LPPPLPHGA PRSPKEVAG SPGVFKAVG 
      REFFPNESP EPNTNGRLL WDFLQQLLN DPAQRYTNY 
      IAWKNRDTG VFKIVDPAG LAKLWGIQK NHLSMNYDK 
      MSRALRYYY RVNILRKVQ GERHCYQFL RNPTELKNI 
      KNISLLRQM SPTNLKPPA QIVPSVTIK PEPVESESE 
      TNRGDYDEK PTDLSITME GPTDLSSSS RHVNIICSP 
      DPPIPPQHH
    CDS sequence
    • >TC007831 RA
      atgaaatta gttccgggc cctctgccg ccgattcct 
      acaagcact tcgatggat cgtctcgcc ttgcctttg 
      cccttcagc cccgactcg ctcctgtgg cggtacccg 
      ctcgggttc gcctcaatt ggggcccaa cagccccct 
      tcttcaccc ctgttggac tacaaaagc caactaccg 
      tcaaaccta gcctcggac ccgagagtt tggtcacga 
      gaagacgtt gccacgttt ctacgctgg gctgaacgt 
      gaattcgac ctgcagccc atcgacatg gacatgttc 
      cagatgaac ggcaaagcg atttgtctc ttgacccga 
      acggacttg gccgagaga gccccaggt tcgggcgac 
      gtcctctac aacgtgctc cagctcctg gtcagggat 
      gccaacaac ttgcagcga atgttgccg tccagtcca 
      gtcacccct acctcacgt cacccctat cccttatcg 
      ccacactcg catcctcca acccctaat tggaatatc 
      ctagctcag aacagtgat ccgttccat ccctcgcat 
      gctgcgtcc ctcgccgca cacttgatg gctcagagc 
      aattcggtg actttgagt ccagcgccc tctgtggat 
      agccaagcc ggcagcccc caacatgcc gataatccg 
      caaacgttc ggccattcc attttcagt acaggggcg 
      agtaacgga agcggaagc aacccgtcc gattcggac 
      gaagatgga tacgagaaa aacggacag accggaagt 
      cccccgaac acgcccagt gcgtactcg ctgccgcca 
      ccactgcca catggggcc cccaggagt ccgaaggag 
      gttgcgggg agtcccggg gtgttcaag gctgtgggg 
      agggagttt ttcccgaat gaatctcca gaacccaac 
      acaaatggg cgtttgttg tgggatttc ctccaacaa 
      ctcctcaac gacccagct caacgttac accaattac 
      atcgcctgg aaaaaccgt gatactggc gtgttcaaa 
      atcgttgat ccggctgga ttggccaag ctttggggc 
      atccagaag aatcacctt tcaatgaac tacgacaaa 
      atgtcacgt gccttgcgg tattactac agagtgaat 
      attttaagg aaagtgcaa ggagagcga cactgctac 
      cagtttttg cgcaacccg accgagctg aagaacatc 
      aagaacatc tcgttgttg cggcaaatg tcgccgaca 
      aatctgaag cctccagcg cagatcgtc cccagtgtg 
      accatcaag ccggagcct gtggagagt gaaagcgag 
      acaaatcgg ggtgattat gacgagaaa ccgaccgat 
      ttgagcata acaatggag gggcccacc gacttgagc 
      agtagtagc cgacatgtg aacataatc tgttcgccg 
      gatccgccc ataccgccc cagcatcac tag
    mRNA sequence
    • >TC007831 RA
      gcagttccc aaaacagta gtggtaagg tggcggctg 
      agtgagcaa cgtcacagt tactgctag tgctcgatt 
      ggcttgtta gagtgtgtg agaaataaa aacagtgca 
      agggttgat gtcacggat taaacaaca acaataaca 
      agattgaaa atatgaaat tagttccgg gccctctgc 
      cgccgattc ctacaagca cttcgatgg atcgtctcg 
      ccttgcctt tgcccttca gccccgact cgctcctgt 
      ggcggtacc cgctcgggt tcgcctcaa ttggggccc 
      aacagcccc cttcttcac ccctgttgg actacaaaa 
      gccaactac cgtcaaacc tagcctcgg acccgagag 
      tttggtcac gagaagacg ttgccacgt ttctacgct 
      gggctgaac gtgaattcg acctgcagc ccatcgaca 
      tggacatgt tccagatga acggcaaag cgatttgtc 
      tcttgaccc gaacggact tggccgaga gagccccag 
      gttcgggcg acgtcctct acaacgtgc tccagctcc 
      tggtcaggg atgccaaca acttgcagc gaatgttgc 
      cgtccagtc cagtcaccc ctacctcac gtcacccct 
      atcccttat cgccacact cgcatcctc caaccccta 
      attggaata tcctagctc agaacagtg atccgttcc 
      atccctcgc atgctgcgt ccctcgccg cacacttga 
      tggctcaga gcaattcgg tgactttga gtccagcgc 
      cctctgtgg atagccaag ccggcagcc cccaacatg 
      ccgataatc cgcaaacgt tcggccatt ccattttca 
      gtacagggg cgagtaacg gaagcggaa gcaacccgt 
      ccgattcgg acgaagatg gatacgaga aaaacggac 
      agaccggaa gtcccccga acacgccca gtgcgtact 
      cgctgccgc caccactgc cacatgggg cccccagga 
      gtccgaagg aggttgcgg ggagtcccg gggtgttca 
      aggctgtgg ggagggagt ttttcccga atgaatctc 
      cagaaccca acacaaatg ggcgtttgt tgtgggatt 
      tcctccaac aactcctca acgacccag ctcaacgtt 
      acaccaatt acatcgcct ggaaaaacc gtgatactg 
      gcgtgttca aaatcgttg atccggctg gattggcca 
      agctttggg gcatccaga agaatcacc tttcaatga 
      actacgaca aaatgtcac gtgccttgc ggtattact 
      acagagtga atattttaa ggaaagtgc aaggagagc 
      gacactgct accagtttt tgcgcaacc cgaccgagc 
      tgaagaaca tcaagaaca tctcgttgt tgcggcaaa 
      tgtcgccga caaatctga agcctccag cgcagatcg 
      tccccagtg tgaccatca agccggagc ctgtggaga 
      gtgaaagcg agacaaatc ggggtgatt atgacgaga 
      aaccgaccg atttgagca taacaatgg aggggccca 
      ccgacttga gcagtagta gccgacatg tgaacataa 
      tctgttcgc cggatccgc ccataccgc cccagcatc 
      actagcgac agtgacaat aacgtgcat tattagtga 
      caatactgg tgccaatat ttataagac ttgtgcaaa 
      ggaatagtg atgataaaa aatgtgtat atagttaat 
      tttttaaga agaagaata aaaatatat tttggattt 
      ttaaagcgg agggcttta gaaatcttt catacgcat 
      gtgccaaaa taaattatt ttttcattt ggtcacgag 
      ggtgactgc cgaaggcag ctttttggg tgatcctca 
      agcaatgac aataagtaa attcaatgt acaaagaag 
      ttggactgg aaggaaatt gatttttgt accacattc 
      caaacttgc ggcatttgc ccctttcac atggaaaat 
      tcacgcgtg gctgaactg gttccagtt ctatttctg 
      tgatctctt aatgtttga ctcaatttt ttgtgtgat 
      tttaaaaag aatgtataa tgctttatg tattctgta 
      catattttt acgcatatt caaagataa tagtcttaa 
      gtttgtaag ggtggataa cctaatggc tttattttt 
      aaacgagaa tcgaatttt gtgtcaatt ggtatattg 
      ttcccatgt tatcagttt tgtacatat gttataaat 
      tagcaacct tcaaataca acgctgtcc taatatgtt 
      ttgtatgag aatttattg ttaacgaag atatgacaa 
      gtttgaata tacctttac agcaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY