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Gene information
General information
TC007805 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 885863..887845
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007805
    iBeetle screen iB_01269
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007805 RA
      MEASLASTD TVIDEEAKA GGPQPLSKL QVNGITAGD 
      LKKLEDAGF HSIESIAYT PKKVLAAIK GISEAKVNK 
      LLAEAAKFV NMGFTTATD IHRRRANII QLTTGSKEL 
      DKLLGGGIE TGSITEIFG EFRTGKTQI CHTLAVTCQ 
      LSVESGGGE GKCLYIDTE GTFRPERLL PVAERYNMD 
      GQQVLDNVV FARAFNTDH QTELLVHAA AMMMESRYC 
      LLIVDSATS LYRTDYSGR GELSARQNH LARFLRMLL 
      RLADEFGVA VVLTNQVVA QVDGAAMFN ADPKKPIGG 
      NIMAHSSTT RLSLRKGRG EARVCKIYD SPCLPESEA 
      IFAIRPDGI GDVQE
    CDS sequence
    • >TC007805 RA
      atggaggct agtttggca tcaaccgac accgtaatt 
      gacgaggaa gccaaagcc ggcggcccc cagcccctt 
      tccaagctt caggtcaac gggatcaca gctggcgac 
      ttgaagaag ctcgaagat gccggtttt cactcaatt 
      gaatcaatt gcatacact cctaaaaaa gttcttgcg 
      gctattaaa ggcatctct gaagccaaa gtcaataaa 
      ctcctcgcc gaagcggcg aaatttgtt aatatgggt 
      ttcaccaca gccactgat attcacaga cgaagggct 
      aatattatc caactgacc accggttcg aaagaattg 
      gataagttg ttagggggc ggtattgaa actggttca 
      attacggaa attttcggc gaatttcga acggggaaa 
      acacaaatt tgccacact ttggctgtg acttgtcaa 
      ttgtcggtt gaaagtggc ggaggcgaa ggcaagtgt 
      ctttatatt gacactgag ggcacgttc aggcctgaa 
      cgcttgcta ccggtggct gagaggtac aacatggac 
      ggacaacaa gtcctggat aatgtcgtg tttgcgaga 
      gcttttaat accgatcat cagactgaa cttttggtt 
      catgccgcg gcaatgatg atggagtca aggtactgt 
      cttttgatt gtcgatagt gccacgtct ttgtatcgg 
      acggattat tcgggaagg ggggaattg tcagcaagg 
      cagaatcat ctggcgagg tttctgagg atgttgctg 
      cgacttgct gatgagttt ggagttgcc gttgtgctg 
      acgaaccaa gtggttgcc caagttgac ggcgctgcg 
      atgtttaac gcagaccct aagaaacca atcggtggt 
      aacattatg gcacattca tccacaaca cgactttcc 
      ttgaggaaa ggccgaggc gaagcaaga gtgtgcaaa 
      atatacgat tcgccgtgt ttgcccgaa tccgaggca 
      atttttgcc attcgtccg gacggaatc ggggatgtc 
      caggaatga
    mRNA sequence
    • >TC007805 RA
      ccacttttt aaattttgt tatggtttt tggttttat 
      ttgcctggc cttggcggg actttttgt ttgaattcc 
      taaccaaag aaacagccc actatcaca acaatcagc 
      agtgaacta aaaaacgaa atttcttta ctaaatcat 
      ctaaatgga ggctagttt ggcatcaac cgacaccgt 
      aattgacga ggaagccaa agccggcgg cccccagcc 
      cctttccaa gcttcaggt caacgggat cacagctgg 
      cgacttgaa gaagctcga agatgccgg ttttcactc 
      aattgaatc aattgcata cactcctaa aaaagttct 
      tgcggctat taaaggcat ctctgaagc caaagtcaa 
      taaactcct cgccgaagc ggcgaaatt tgttaatat 
      gggtttcac cacagccac tgatattca cagacgaag 
      ggctaatat tatccaact gaccaccgg ttcgaaaga 
      attggataa gttgttagg gggcggtat tgaaactgg 
      ttcaattac ggaaatttt cggcgaatt tcgaacggg 
      gaaaacaca aatttgcca cactttggc tgtgacttg 
      tcaattgtc ggttgaaag tggcggagg cgaaggcaa 
      gtgtcttta tattgacac tgagggcac gttcaggcc 
      tgaacgctt gctaccggt ggctgagag gtacaacat 
      ggacggaca acaagtcct ggataatgt cgtgtttgc 
      gagagcttt taataccga tcatcagac tgaactttt 
      ggttcatgc cgcggcaat gatgatgga gtcaaggta 
      ctgtctttt gattgtcga tagtgccac gtctttgta 
      tcggacgga ttattcggg aagggggga attgtcagc 
      aaggcagaa tcatctggc gaggtttct gaggatgtt 
      gctgcgact tgctgatga gtttggagt tgccgttgt 
      gctgacgaa ccaagtggt tgcccaagt tgacggcgc 
      tgcgatgtt taacgcaga ccctaagaa accaatcgg 
      tggtaacat tatggcaca ttcatccac aacacgact 
      ttccttgag gaaaggccg aggcgaagc aagagtgtg 
      caaaatata cgattcgcc gtgtttgcc cgaatccga 
      ggcaatttt tgccattcg tccggacgg aatcgggga 
      tgtccagga atgatattt tattttttt actagattg 
      tgcgttatt tttaatttt gctgataat aagtatttt 
      tccaattga tattatata tttcacaat aggtaactt 
      aaatttgtc cagccaatt tcccttcaa attttacga 
      atatttgac tctcagggt gttaaaagt aaaacctat 
      gcaaattga ataacccgt gacattagc ctgtccccg 
      attcccccc atgttttcc acaatgtaa cggaatccc 
      aaacgctgc tagtttcat aaatcacac aatcgaaaa 
      aatcgcaaa tagcctcca tctcccttc aaggtccct 
      gagcaagtg tacctactc tttccggct ggtgtgagt 
      cgaattgcc cagattata taacaattt tcggtggaa 
      attggccgc gtggcgcga caaattggc cgtttttgt 
      cactttttc acgtgatga atttccatc tcgactgag 
      gatcgagtc atttcaatc ctcaatcgc catttctcg 
      aggcaataa ccgtaatat agtaaccca attccgcgt 
      ggctagact ttcatctta agtattttg tttgaaaaa 
      aataacgtt ttatcaaat gacgaggcg atgaattat 
      ttatggaca agggcctga gcgaaaact gggacgtta 
      tttcgagtt gcgcaacgc gggccgcac acgtgctgg 
      aattttgaa tcagcggac aagtctggg aaccactgt 
      aatataacg aaattcaca ag