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Gene information
General information
TC007809 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 919193..924551
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007809
    iBeetle screen iB_01268

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007809 RA
      MFGQENANE MAFLTDKLL KEGYMVKRS QNKKVYTLV 
      NYKKRWFVL TRRSLIYYD MQNESKRKE KGRIDLNSV 
      YCIEPAVLS ATPEETGIQ GDFYPFQIG YVSDDHYIL 
      YLVAVKDCE RSDWIQALR KACANNFYV RNFHHSGFW 
      NGKKWTCCQ KNFKGNEGC KTCTSWLQD EIKDEDNSS 
      AQSSPIMPG GATPGTPST KQKEKAMGR TKVVVALYN 
      YKAIESGDL SLEKNQEYE VIDDSQEHW WKVKDSKGN 
      IGFIPSNYV KEKELLGLQ QYEWYVNDM SRQRSESLL 
      KQEDKEGCF VVRNSSTKG LYTLSLYTK IPHPHVKHY 
      HIKQNSRGE FYLSEKHCC NSIPDLINY HKHNSGGLA 
      SRLKTSPCD RPAPATAGL SHDKWEIDH NELMLLEEL 
      GSGQFGVVR RGKWRGSID VAVKMMKEG TMSEDDFIE 
      EAKVMTKLQ HTNLVQLYG VCSKNRPIF IVTEYMRYG 
      SLLNHLRRH EQSLSINQG LLLDMCIQI CKGMAYLER 
      HNYIHRDLA ARNCLVGTE NIVKVADFG LARYVLDDQ 
      YTSSGGSKF PIKWAPPEV LNYTRFSSK SDVWAYGVL 
      MWEVFTCGK MPYGRLKNS EVVERVQKG LILEKPKAC 
      YKEVYDIMR KCWSHLPEN RPSFRVLKE QLVSASQGI 
      LVD
    CDS sequence
    • >TC007809 RA
      atgttcggc caggaaaat gccaacgaa atggccttc 
      ctcaccgac aagctcctc aaggagggc tacatggtc 
      aagcgctcg caaaacaag aaggtctac acgctggtc 
      aactacaag aagcgctgg ttcgtcctc accaggagg 
      agtctcatc tactacgac atgcagaat gagagtaaa 
      cgtaaagaa aagggccga attgatttg aactcggtc 
      tactgcatc gagccggcc gtgctttca gcaacccct 
      gaagagacg ggcatccag ggtgacttc taccccttt 
      caaattggc tatgtctcc gatgaccac tacatcctc 
      tatctcgtt gctgtgaaa gactgcgaa cgctccgac 
      tggatccaa gcattaaga aaagcctgt gcgaataat 
      ttctacgtt cgaaatttc catcattcc ggattctgg 
      aacggtaag aagtggacc tgttgccag aagaatttc 
      aaagggaac gaaggctgc aagacttgc actagttgg 
      ctacaggac gaaatcaaa gacgaggac aactcgagt 
      gcacagtcc agtccgata atgccaggt ggtgctact 
      ccaggcacc ccttcaacc aagcaaaag gaaaaggca 
      atgggacgg actaaagtc gttgtggca ctttataat 
      tacaaagcg atcgaaagt ggggatctc tccttggag 
      aaaaatcag gaatacgaa gttatcgat gattcgcaa 
      gagcattgg tggaaagtt aaagattcg aaaggaaat 
      atcggattc attccgagt aattacgtc aaagagaaa 
      gaattacta ggattacaa caatacgaa tggtatgtt 
      aatgatatg tcacgacaa agatccgaa tctctgctg 
      aaacaagaa gataaggaa ggttgtttt gttgtgaga 
      aactcctca acgaaaggt ctctacact ttgtctcta 
      tacaccaaa atacctcac ccccatgtt aaacactac 
      cacatcaag cagaactct cgtggcgaa ttctactta 
      tcggaaaag cactgttgt aattcaatc cctgacctt 
      atcaactac cacaaacat aacagtggt ggtttggct 
      tcacgatta aaaactagt ccttgtgat aggccagcg 
      cccgcaacg gctggactt agccacgac aagtgggaa 
      attgatcat aacgaattg atgctattg gaggaattg 
      ggtagcgga cagtttgga gtagtcaga aggggtaaa 
      tggaggggt tcgatcgat gtcgctgtg aaaatgatg 
      aaagagggg acgatgtct gaggatgat ttcattgag 
      gaagcaaaa gtcatgacg aaactgcaa catacgaat 
      ttggtgcag ttgtacggg gtgtgtagc aagaatcgg 
      cccattttc atcgtgact gaatacatg aggtatggt 
      tcattgttg aatcattta cgacgacac gagcaatca 
      ttatcgatt aatcaaggc ttgttactt gatatgtgt 
      attcaaatt tgtaaagga atggcctac ttggagcga 
      cacaattac atacatcgt gacttggcc gctcgtaat 
      tgtctagta ggtactgaa aatatcgta aaagtggcc 
      gattttggg ctggctagg tacgttttg gacgatcaa 
      tatacatca tcaggaggg tcgaaattc ccgatcaag 
      tgggcaccg cctgaagta ttaaattat acgaggttt 
      tcctccaaa tcggacgtt tgggcttac ggtgtgtta 
      atgtgggag gtgttcacg tgcgggaaa atgccctac 
      ggtagatta aaaaattcc gaagttgtg gagcgtgtc 
      cagaaaggc ctaattttg gaaaagccg aaagcgtgc 
      tacaaggag gtctacgat ataatgagg aaatgttgg 
      agtcattta ccggaaaac cggccgtct ttccgcgtt 
      ttaaaggag caattggtc agcgcatcg caaggtatt 
      ctggtcgat tga
    mRNA sequence
    • >TC007809 RA
      gtcggtttg ttatcaaac agtgtcgac cttcaaata 
      cccagtggt ggccgggaa atggtttga atgcctcga 
      cttaaccta agtgtcgat gttcggcca ggaaaatgc 
      caacgaaat ggccttcct caccgacaa gctcctcaa 
      ggagggcta catggtcaa gcgctcgca aaacaagaa 
      ggtctacac gctggtcaa ctacaagaa gcgctggtt 
      cgtcctcac caggaggag tctcatcta ctacgacat 
      gcagaatga gagtaaacg taaagaaaa gggccgaat 
      tgatttgaa ctcggtcta ctgcatcga gccggccgt 
      gctttcagc aacccctga agagacggg catccaggg 
      tgacttcta cccctttca aattggcta tgtctccga 
      tgaccacta catcctcta tctcgttgc tgtgaaaga 
      ctgcgaacg ctccgactg gatccaagc attaagaaa 
      agcctgtgc gaataattt ctacgttcg aaatttcca 
      tcattccgg attctggaa cggtaagaa gtggacctg 
      ttgccagaa gaatttcaa agggaacga aggctgcaa 
      gacttgcac tagttggct acaggacga aatcaaaga 
      cgaggacaa ctcgagtgc acagtccag tccgataat 
      gccaggtgg tgctactcc aggcacccc ttcaaccaa 
      gcaaaagga aaaggcaat gggacggac taaagtcgt 
      tgtggcact ttataatta caaagcgat cgaaagtgg 
      ggatctctc cttggagaa aaatcagga atacgaagt 
      tatcgatga ttcgcaaga gcattggtg gaaagttaa 
      agattcgaa aggaaatat cggattcat tccgagtaa 
      ttacgtcaa agagaaaga attactagg attacaaca 
      atacgaatg gtatgttaa tgatatgtc acgacaaag 
      atccgaatc tctgctgaa acaagaaga taaggaagg 
      ttgttttgt tgtgagaaa ctcctcaac gaaaggtct 
      ctacacttt gtctctata caccaaaat acctcaccc 
      ccatgttaa acactacca catcaagca gaactctcg 
      tggcgaatt ctacttatc ggaaaagca ctgttgtaa 
      ttcaatccc tgaccttat caactacca caaacataa 
      cagtggtgg tttggcttc acgattaaa aactagtcc 
      ttgtgatag gccagcgcc cgcaacggc tggacttag 
      ccacgacaa gtgggaaat tgatcataa cgaattgat 
      gctattgga ggaattggg tagcggaca gtttggagt 
      agtcagaag gggtaaatg gaggggttc gatcgatgt 
      cgctgtgaa aatgatgaa agaggggac gatgtctga 
      ggatgattt cattgagga agcaaaagt catgacgaa 
      actgcaaca tacgaattt ggtgcagtt gtacggggt 
      gtgtagcaa gaatcggcc cattttcat cgtgactga 
      atacatgag gtatggttc attgttgaa tcatttacg 
      acgacacga gcaatcatt atcgattaa tcaaggctt 
      gttacttga tatgtgtat tcaaatttg taaaggaat 
      ggcctactt ggagcgaca caattacat acatcgtga 
      cttggccgc tcgtaattg tctagtagg tactgaaaa 
      tatcgtaaa agtggccga ttttgggct ggctaggta 
      cgttttgga cgatcaata tacatcatc aggagggtc 
      gaaattccc gatcaagtg ggcaccgcc tgaagtatt 
      aaattatac gaggttttc ctccaaatc ggacgtttg 
      ggcttacgg tgtgttaat gtgggaggt gttcacgtg 
      cgggaaaat gccctacgg tagattaaa aaattccga 
      agttgtgga gcgtgtcca gaaaggcct aattttgga 
      aaagccgaa agcgtgcta caaggaggt ctacgatat 
      aatgaggaa atgttggag tcatttacc ggaaaaccg 
      gccgtcttt ccgcgtttt aaaggagca attggtcag 
      cgcatcgca aggtattct ggtcgattg actggcgca 
      attggtttg cgagtcggt gtcaaagag ctcaacttc 
      gacgtcttc atgtgatct ttgttgcaa ttacgaaca 
      aacctggat cggaatctg cgagactct cagacgaga 
      ggcgcgcaa agccaaaca ataaagact ctcttagct 
      t
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