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Gene information
General information
TC032738
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007807
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 908913..911417
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032738
    iBeetle screen iB_01267

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032738 RA
      MVMEEEEYD EGSRKEPVI VTESKDFDA IEKHVLPKN 
      TKKFDAKEC DVALLDFES LCSFSKNEK KDTLWEHDI 
      RTRRLQRKL FEQDVIIDE MNRKITDFD DKVEKLNTE 
      RFQAEVDAK IIDIFMVTV HQELLILKE FEALEYTLQ 
      SKVNQKMSE VLDMRDIID EISSKIVEH NSDIEKAQE 
      KIKSITVSF TNAVQDNKF FDFLRKVFR KKYKPPKVH 
      KDDESSSES SSSSTSSTE SDDDGKSID SRDFGIIKQ 
      DLNVCPKGC DPSVYEYTI NSRSERHQV ELLIKEVLK 
      MIEVLKKDL EIHNKKVKV IEKDLQTCQ DELEKYQRQ 
      KQDKLNEVR TTVILRLDQ MKHLAEEYK TSGIMDTLI 
      FSKERLSYL YKRVEELQI ETEHQVSRH RLNVKHYTR 
      MQTDCRYMA TRIKYLQEV ITDKMKMKF GKVIDINEI 
      EVAMLKRTF GRDDLHDLE EVLLKKIVH DLRLSMMDI 
      KGLYIDQLH FWKEKISQT QKELTYVLR HNTSQQELL 
      TYLVNEKVE LAQSLMSQE KKKLHIASV GDITRRYSE 
      EIEKLQAII SEQNQQIQE LKDEIKLLR TKGMVLKPK 
      EVKKEEPEE EPVAWEHIK DWGEEIEEE EEESLRKPF 
      STEIVIPTT LQEKSQEVA TELIQEMLE ALDVKLTKR 
      SNENFVKEI LGSVMRGLS MTELVEELV RNLPIEPDD 
      VQRGIIETT AEQLYVVQE PDAEAEDKF FSCREILED 
      IVDEILLVK GEPHSSSLV WNILLKEW
    CDS sequence
    • >TC032738 RA
      atggtaatg gaagaggag gaatatgat gaagggagt 
      aggaaagaa ccggtaatt gtgaccgaa tcgaaagat 
      ttcgatgca attgaaaaa catgttttg ccgaaaaat 
      accaaaaaa tttgacgcg aaagaatgc gatgttgca 
      ctgcttgat ttcgaatca ttgtgtagt ttttccaaa 
      aacgagaaa aaagacact ttgtgggaa cacgatatt 
      cgcacgcgg agacttcaa aggaagctt ttcgagcaa 
      gacgtgatt attgatgaa atgaaccga aaaatcacc 
      gattttgat gataaagtc gaaaagttg aacactgaa 
      agattccaa gccgaggtg gacgcaaaa atcattgat 
      atttttatg gtgacagtg caccaagaa ctgctcatt 
      ctgaaggaa tttgaagct ttggagtac acacttcag 
      tcgaaagtt aatcagaaa atgtccgaa gttttagat 
      atgagagac attatcgac gagatcagc tcgaaaatc 
      gtcgaacat aattccgac atcgagaag gcacaagag 
      aagattaaa tcgataaca gtcagtttc acaaatgcg 
      gttcaagat aacaagttt tttgatttt ttgaggaaa 
      gtgtttcgg aaaaaatac aaaccgcca aaagtgcac 
      aaggatgat gaatcgagc tccgaatca tcatcatcg 
      tcaacgtca tcgacggaa tctgacgat gatggcaaa 
      agtatcgac tcgcgggat ttcgggatt ataaaacag 
      gacttgaac gtttgtcct aaagggtgt gaccctagc 
      gtttacgaa tatacgatc aattcgcgt tcggagaga 
      catcaagtc gaacttttg atcaaagaa gtattaaaa 
      atgattgaa gtgttgaaa aaggacctg gaaatacac 
      aacaagaaa gtgaaagtg attgaaaaa gacttgcaa 
      acgtgtcaa gacgaactg gaaaagtat caaagacag 
      aaacaggat aaattgaac gaagtccgg actaccgtt 
      atcttgcgt ttggatcaa atgaaacat ttggcagag 
      gaatacaaa actagcggg attatggac actcttatc 
      ttttccaaa gagcgtctc tcctatctc tataaacga 
      gttgaagag ttgcaaatt gaaactgaa caccaagtg 
      agccgacac agactcaat gtcaagcac tacactcgc 
      atgcaaacc gattgccga tacatggca acaagaata 
      aaatacttg caggaagta atcaccgat aaaatgaaa 
      atgaaattt ggtaaagtg atcgatatt aacgaaatt 
      gaagtagct atgcttaaa cgcactttt gggcgagac 
      gatttacac gatttggaa gaagtcctc ttgaagaaa 
      atcgtccat gatttgcgc ttatccatg atggatatt 
      aagggcttg tacattgat cagttgcat ttttggaag 
      gaaaaaatc tcgcaaact cagaaggaa ctgacttat 
      gttttacgc cacaatacg tctcagcaa gaactgttg 
      acttatttg gtgaatgag aaagttgag ttggctcag 
      agtctgatg agtcaagag aagaagaaa ctacacatt 
      gccagtgtt ggtgacatt acgcgaaga tacagcgaa 
      gaaatcgaa aaattgcaa gcgattatt tcggaacaa 
      aaccaacaa atccaagaa ttgaaggac gaaattaaa 
      ttattgagg accaaagga atggtcctc aagccgaaa 
      gaagtgaag aaggaagaa ccggaagaa gagccagtg 
      gcttgggag catatcaag gactgggga gaggaaatt 
      gaggaagag gaggaggaa agtttaagg aaaccgttc 
      tcgacggaa attgtgatc ccaacaact ttgcaagag 
      aaatcgcaa gaggtcgct actgaactg atccaagaa 
      atgcttgaa gcgctagac gtgaaactg acgaaacga 
      tcgaatgag aatttcgtt aaggagatt ttggggtcg 
      gtaatgcgt ggcttaagt atgacggaa cttgtcgaa 
      gagttggtc cggaacttg ccgattgaa cccgatgat 
      gttcaaagg ggtattatt gagactaca gccgagcaa 
      ttgtacgtg gtccaagaa ccagatgct gaagctgaa 
      gataaattc ttttcttgt cgggaaatt ctcgaagat 
      atcgttgat gaaattcta ctagttaaa ggggagccg 
      cattcgagc agtctagtt tggaatata ttgttaaaa 
      gaatggtaa
    mRNA sequence
    • >TC032738 RA
      gcattttga aattaccgt gactatggt aatggaaga 
      ggaggaata tgatgaagg gagtaggaa agaaccggt 
      aattgtgac cgaatcgaa agatttcga tgcaattga 
      aaaacatgt tttgccgaa aaataccaa aaaatttga 
      cgcgaaaga atgcgatgt tgcactgct tgatttcga 
      atcattgtg tagtttttc caaaaacga gaaaaaaga 
      cactttgtg ggaacacga tattcgcac gcggagact 
      tcaaaggaa gcttttcga gcaagacgt gattattga 
      tgaaatgaa ccgaaaaat caccgattt tgatgataa 
      agtcgaaaa gttgaacac tgaaagatt ccaagccga 
      ggtggacgc aaaaatcat tgatatttt tatggtgac 
      agtgcacca agaactgct cattctgaa ggaatttga 
      agctttgga gtacacact tcagtcgaa agttaatca 
      gaaaatgtc cgaagtttt agatatgag agacattat 
      cgacgagat cagctcgaa aatcgtcga acataattc 
      cgacatcga gaaggcaca agagaagat taaatcgat 
      aacagtcag tttcacaaa tgcggttca agataacaa 
      gttttttga ttttttgag gaaagtgtt tcggaaaaa 
      atacaaacc gccaaaagt gcacaagga tgatgaatc 
      gagctccga atcatcatc atcgtcaac gtcatcgac 
      ggaatctga cgatgatgg caaaagtat cgactcgcg 
      ggatttcgg gattataaa acaggactt gaacgtttg 
      tcctaaagg gtgtgaccc tagcgttta cgaatatac 
      gatcaattc gcgttcgga gagacatca agtcgaact 
      tttgatcaa agaagtatt aaaaatgat tgaagtgtt 
      gaaaaagga cctggaaat acacaacaa gaaagtgaa 
      agtgattga aaaagactt gcaaacgtg tcaagacga 
      actggaaaa gtatcaaag acagaaaca ggataaatt 
      gaacgaagt ccggactac cgttatctt gcgtttgga 
      tcaaatgaa acatttggc agaggaata caaaactag 
      cgggattat ggacactct tatcttttc caaagagcg 
      tctctccta tctctataa acgagttga agagttgca 
      aattgaaac tgaacacca agtgagccg acacagact 
      caatgtcaa gcactacac tcgcatgca aaccgattg 
      ccgatacat ggcaacaag aataaaata cttgcagga 
      agtaatcac cgataaaat gaaaatgaa atttggtaa 
      agtgatcga tattaacga aattgaagt agctatgct 
      taaacgcac ttttgggcg agacgattt acacgattt 
      ggaagaagt cctcttgaa gaaaatcgt ccatgattt 
      gcgcttatc catgatgga tattaaggg cttgtacat 
      tgatcagtt gcatttttg gaaggaaaa aatctcgca 
      aactcagaa ggaactgac ttatgtttt acgccacaa 
      tacgtctca gcaagaact gttgactta tttggtgaa 
      tgagaaagt tgagttggc tcagagtct gatgagtca 
      agagaagaa gaaactaca cattgccag tgttggtga 
      cattacgcg aagatacag cgaagaaat cgaaaaatt 
      gcaagcgat tatttcgga acaaaacca acaaatcca 
      agaattgaa ggacgaaat taaattatt gaggaccaa 
      aggaatggt cctcaagcc gaaagaagt gaagaagga 
      agaaccgga agaagagcc agtggcttg ggagcatat 
      caaggactg gggagagga aattgagga agaggagga 
      ggaaagttt aaggaaacc gttctcgac ggaaattgt 
      gatcccaac aactttgca agagaaatc gcaagaggt 
      cgctactga actgatcca agaaatgct tgaagcgct 
      agacgtgaa actgacgaa acgatcgaa tgagaattt 
      cgttaagga gattttggg gtcggtaat gcgtggctt 
      aagtatgac ggaacttgt cgaagagtt ggtccggaa 
      cttgccgat tgaacccga tgatgttca aaggggtat 
      tattgagac tacagccga gcaattgta cgtggtcca 
      agaaccaga tgctgaagc tgaagataa attcttttc 
      ttgtcggga aattctcga agatatcgt tgatgaaat 
      tctactagt taaagggga gccgcattc gagcagtct 
      agtttggaa tatattgtt aaaagaatg gtaacaagc 
      gaattagaa accaagttg aaccgttcc gatgttatg 
      agct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY