Status
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Gene information
General information
TC007806 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 890765..894854
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC007806
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01266

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
       larva appendages irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      legs irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 77.78% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007806 RA
        MGTAMPSAP AAQGFLPTV VLQMLQCPQ CLKLDQSFL 
KRENAVPKT FSVSVSDIG CDSRNSGTI WNHHNSKPT 
FRGENMRKI KRKDQQLLT NFVKNNLED LNTPNKTIK 
VGTKNKVRV GTTTRKTTK LTPNPIVIN ITAPPKKPT 
KVVRVTKPP FKIKLTPTT KKPKIKPTI RRVVTKWPN 
EVKQNWYEQ GVPYPTPNP EINSHSPPY SLSEVASDP 
GQVSEPPQT DLSNAISTF NLDMAPDPS NIRDGAGGS 
PCPTVHISS SVLTPQQRQ DCSDLNLVI NSHFHQNSV 
TDRSPLTPE TYDAANQAQ QEDPAPPPQ TQADPGGGA 
GGASQAAAP AAPGGGTGG SGGSGGNGG DGDDGGGLK 
LPDLKGLMD LLGWLWDKL GHLFNFLKN PYLYLVPMV 
LFFLLGFLA VILLFPWWI PALFLFASA KTAQKKNVA 
FYKHVHKPV HHPDGWFWN HETKTWQNL AEIVHHHHH 
HRRSDGDGD SGEDNYKEI TDAIENFSR KYESSSQSW 
KWRRRVRSP SQIGETIKQ VTSQLMEAN ATSPDIVTL 
DTRTPPKIK QHAKKKPQK PETLENSHA GVAIPVNED 
EINLEETTK KFFYKIEKP TTNSGLSTW ILLSGQTTA 
KPSRKPVVK PNESQNVTV VVDSNKIIK PLFKKRGTT 
TSTTTLAPT TTKLTKVKA SVLSGAKSS TTTTTTMTT 
PTTSSTTTT TNSSTLPIE AKNSETDLS EKKTRRPST 
TKRKKNKNR RRRPSEKTD SSTKIDKPI QSKEKPIGT 
QLYNYLSRE VMPTVGVGL VGLMVTAGL AGYFLYPFG 
VARRSYDID RRDKDNHFY YSDEYSGGI AEEEAIGKV 
IAGMPSNSL YGSNFKTPT SRHSFNNHR VIEQSTVGG 
EGATPAAVP EHGPRKRRQ VNDGENELD GSITTDDGP 
STTTTTTST THRPDKVKS LVDMFKELF HLKINLGLQ 
LLQNATQAV SKYVSHVQK RLDEHYRNY TLTNSTN
      CDS sequence
      • >TC007806 RA
        atgggtacc gctatgccg agcgcaccg gccgctcag 
gggttcctg ccaacagta gttcttcag atgttacag 
tgcccacag tgtcttaaa ctcgaccag tcgttttta 
aaacgtgaa aatgcagtg cccaagacc tttagtgtt 
agtgttagt gacatcggc tgtgatagc cgcaactcc 
ggtaccatc tggaaccac cacaacagc aagcccact 
tttcgcggc gaaaatatg cgcaaaatt aaacgaaaa 
gaccaacaa ctactcaca aactttgtc aaaaataat 
ttggaagat ctaaacacc cctaacaaa acgatcaaa 
gttggtacc aaaaacaag gttcgtgtt ggtaccacc 
actcggaaa accacgaaa cttacccca aaccctata 
gttattaac attactgca ccccccaaa aaaccgaca 
aaggtggta cgcgtaacc aaaccccct ttcaaaatc 
aaactaacc ccaaccacc aaaaaaccc aaaataaaa 
ccaacaatt agacgcgtt gtcacaaaa tggcccaat 
gaagtcaaa cagaattgg tacgaacaa ggggtacca 
taccccacc cccaacccc gaaatcaac tcacactcg 
cccccttac tccctcagc gaggtcgct tcagaccct 
ggccaggtc tcagaaccc ccccagact gacctaagc 
aatgctatc agtaccttt aatctggac atggccccc 
gacccctcc aacatccga gatggggcc gggggttcc 
ccttgcccc acagtccac atttcaagc tcggtccta 
accccccaa caacgccaa gactgttcc gacttaaac 
ctcgtcata aactcccat ttccatcaa aattcagta 
acagaccgg tcccccctt acccccgaa acgtacgac 
gcagccaac caggcccag caggaagac ccggcaccg 
cccccgcaa actcaagcc gatcctggg ggcggcgcc 
ggcggcgcc tcacaagcc gccgctccc gcggccccc 
ggtggtggt accggaggg agcggcggt agtggcggt 
aatgggggc gatggcgat gatggaggt ggtctcaaa 
ttaccagat ttgaaaggt ttgatggat ttgttgggc 
tggttgtgg gacaagttg ggccacctc ttcaatttt 
ttgaaaaat ccctacttg tatctggta ccaatggta 
ctgtttttc ttgctgggt ttcttggcg gtcattttg 
ttgttcccc tggtggatc ccggcgttg tttttgttc 
gccagtgcg aaaacagca cagaaaaaa aatgtcgcg 
ttttataaa catgtccat aaaccggtg catcaccca 
gatgggtgg ttttggaac catgagact aaaacctgg 
cagaatttg gccgaaatc gtgcatcat catcatcat 
catcgaagg tcggatggt gatggtgac agtggtgaa 
gataattat aaagaaata acagatgct atagaaaat 
ttctcgcgg aaatatgag agtagtagt cagtcgtgg 
aagtggcgg agaagggtc agatctccg agccaaatt 
ggagaaaca ataaagcaa gtgactagt cagctaatg 
gaagcaaat gcaacctca cccgatata gtcacactc 
gatacacga acaccgccc aaaatcaaa cagcacgcg 
aaaaagaag ccacaaaaa ccagaaacc ctcgaaaat 
tcacatgca ggggttgcc ataccggtc aacgaagac 
gagataaat ctcgaagaa acgaccaaa aaattcttc 
tacaagatt gaaaaaccc acaactaac agtggccta 
tcgacttgg atcctgtta agcgggcag acaacagcc 
aaaccgtcc cgaaaaccg gtcgtgaaa ccaaatgag 
agccaaaat gtcacggtt gtggtggat tctaacaaa 
atcatcaag cctttgttc aaaaaacgt ggtaccact 
actagtacc acaacactg gccccaact accaccaaa 
ctgaccaag gtgaaggct tcagtcttg tcgggtgcc 
aagtcaagt accaccacc acaacaacc atgacaaca 
ccaacaact agtagtacc accaccaca actaactcc 
agtacgtta ccaattgaa gcaaaaaat tccgagact 
gatttaagt gagaagaaa acgcgccga ccttccacc 
accaaaagg aagaagaat aagaaccgt cgcagacgc 
cctagcgaa aaaaccgac agtagtacc aaaattgac 
aaacccatc cagtccaaa gaaaaaccc atcggaacc 
caactttac aattacttg tctcgggaa gtgatgccc 
acggttggg gtcggtctc gtgggcttg atggtcact 
gctggactt gctgggtac tttttgtac ccatttgga 
gtcgctcgc aggtcgtac gacatcgac agacgggac 
aaagacaac cacttttac tactcggat gagtactcg 
ggtgggatc gctgaggag gaagccatc gggaaagtg 
atagcggga atgccgtcc aactctttg tacggaagc 
aacttcaaa acgcccact tccagacac agttttaac 
aaccatagg gtaattgag caaagtact gttggtgga 
gagggggca actccggct gcggtaccc gaacacggg 
cccagaaaa cgcagacaa gttaacgat ggagagaat 
gaacttgat ggtagtatt accacggat gatggtccc 
agtactacc accaccaca accagtacc acgcacagg 
ccggacaaa gtcaagtct ttggtggac atgttcaag 
gagttgttc cacttgaag ataaacctt ggactccag 
ttgctacaa aacgcaact caggccgtc tcaaagtac 
gtttctcac gtgcaaaaa cgcctggac gagcattac 
cgcaactac acccttacc aatagtacc aactga
      mRNA sequence
      • >TC007806 RA
        tttcaggta ccagccatg ggtaccgct atgccgagc 
gcaccggcc gctcagggg ttcctgcca acagtagtt 
cttcagatg ttacagtgc ccacagtgt cttaaactc 
gaccagtcg tttttaaaa cgtgaaaat gcagtgccc 
aagaccttt agtgttagt gttagtgac atcggctgt 
gatagccgc aactccggt accatctgg aaccaccac 
aacagcaag cccactttt cgcggcgaa aatatgcgc 
aaaattaaa cgaaaagac caacaacta ctcacaaac 
tttgtcaaa aataatttg gaagatcta aacacccct 
aacaaaacg atcaaagtt ggtaccaaa aacaaggtt 
cgtgttggt accaccact cggaaaacc acgaaactt 
accccaaac cctatagtt attaacatt actgcaccc 
cccaaaaaa ccgacaaag gtggtacgc gtaaccaaa 
ccccctttc aaaatcaaa ctaacccca accaccaaa 
aaacccaaa ataaaacca acaattaga cgcgttgtc 
acaaaatgg cccaatgaa gtcaaacag aattggtac 
gaacaaggg gtaccatac cccaccccc aaccccgaa 
atcaactca cactcgccc ccttactcc ctcagcgag 
gtcgcttca gaccctggc caggtctca gaacccccc 
cagactgac ctaagcaat gctatcagt acctttaat 
ctggacatg gcccccgac ccctccaac atccgagat 
ggggccggg ggttcccct tgccccaca gtccacatt 
tcaagctcg gtcctaacc ccccaacaa cgccaagac 
tgttccgac ttaaacctc gtcataaac tcccatttc 
catcaaaat tcagtaaca gaccggtcc ccccttacc 
cccgaaacg tacgacgca gccaaccag gcccagcag 
gaagacccg gcaccgccc ccgcaaact caagccgat 
cctgggggc ggcgccggc ggcgcctca caagccgcc 
gctcccgcg gcccccggt ggtggtacc ggagggagc 
ggcggtagt ggcggtaat gggggcgat ggcgatgat 
ggaggtggt ctcaaatta ccagatttg aaaggtttg 
atggatttg ttgggctgg ttgtgggac aagttgggc 
cacctcttc aattttttg aaaaatccc tacttgtat 
ctggtacca atggtactg tttttcttg ctgggtttc 
ttggcggtc attttgttg ttcccctgg tggatcccg 
gcgttgttt ttgttcgcc agtgcgaaa acagcacag 
aaaaaaaat gtcgcgttt tataaacat gtccataaa 
ccggtgcat cacccagat gggtggttt tggaaccat 
gagactaaa acctggcag aatttggcc gaaatcgtg 
catcatcat catcatcat cgaaggtcg gatggtgat 
ggtgacagt ggtgaagat aattataaa gaaataaca 
gatgctata gaaaatttc tcgcggaaa tatgagagt 
agtagtcag tcgtggaag tggcggaga agggtcaga 
tctccgagc caaattgga gaaacaata aagcaagtg 
actagtcag ctaatggaa gcaaatgca acctcaccc 
gatatagtc acactcgat acacgaaca ccgcccaaa 
atcaaacag cacgcgaaa aagaagcca caaaaacca 
gaaaccctc gaaaattca catgcaggg gttgccata 
ccggtcaac gaagacgag ataaatctc gaagaaacg 
accaaaaaa ttcttctac aagattgaa aaacccaca 
actaacagt ggcctatcg acttggatc ctgttaagc 
gggcagaca acagccaaa ccgtcccga aaaccggtc 
gtgaaacca aatgagagc caaaatgtc acggttgtg 
gtggattct aacaaaatc atcaagcct ttgttcaaa 
aaacgtggt accactact agtaccaca acactggcc 
ccaactacc accaaactg accaaggtg aaggcttca 
gtcttgtcg ggtgccaag tcaagtacc accaccaca 
acaaccatg acaacacca acaactagt agtaccacc 
accacaact aactccagt acgttacca attgaagca 
aaaaattcc gagactgat ttaagtgag aagaaaacg 
cgccgacct tccaccacc aaaaggaag aagaataag 
aaccgtcgc agacgccct agcgaaaaa accgacagt 
agtaccaaa attgacaaa cccatccag tccaaagaa 
aaacccatc ggaacccaa ctttacaat tacttgtct 
cgggaagtg atgcccacg gttggggtc ggtctcgtg 
ggcttgatg gtcactgct ggacttgct gggtacttt 
ttgtaccca tttggagtc gctcgcagg tcgtacgac 
atcgacaga cgggacaaa gacaaccac ttttactac 
tcggatgag tactcgggt gggatcgct gaggaggaa 
gccatcggg aaagtgata gcgggaatg ccgtccaac 
tctttgtac ggaagcaac ttcaaaacg cccacttcc 
agacacagt tttaacaac catagggta attgagcaa 
agtactgtt ggtggagag ggggcaact ccggctgcg 
gtacccgaa cacgggccc agaaaacgc agacaagtt 
aacgatgga gagaatgaa cttgatggt agtattacc 
acggatgat ggtcccagt actaccacc accacaacc 
agtaccacg cacaggccg gacaaagtc aagtctttg 
gtggacatg ttcaaggag ttgttccac ttgaagata 
aaccttgga ctccagttg ctacaaaac gcaactcag 
gccgtctca aagtacgtt tctcacgtg caaaaacgc 
ctggacgag cattaccgc aactacacc cttaccaat 
agtaccaac tgattattt atgtgaagc tagtccagg 
aggtaatgt ttttggtac cagacaaag tcactacct 
cctggacta tcaggattt atgtactcc cctgtctac 
acttaacag tattcttga gagtacgta gattagttt 
actggggca ggcgtcggg aataaacca taaaaacca 
aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY