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Gene information
General information
TC007806 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 890765..894854
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007806
      iBeetle screen iB_01266
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      larva appendages irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      legs irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 77.78% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007806 RA
        MGTAMPSAP AAQGFLPTV VLQMLQCPQ CLKLDQSFL 
        KRENAVPKT FSVSVSDIG CDSRNSGTI WNHHNSKPT 
        FRGENMRKI KRKDQQLLT NFVKNNLED LNTPNKTIK 
        VGTKNKVRV GTTTRKTTK LTPNPIVIN ITAPPKKPT 
        KVVRVTKPP FKIKLTPTT KKPKIKPTI RRVVTKWPN 
        EVKQNWYEQ GVPYPTPNP EINSHSPPY SLSEVASDP 
        GQVSEPPQT DLSNAISTF NLDMAPDPS NIRDGAGGS 
        PCPTVHISS SVLTPQQRQ DCSDLNLVI NSHFHQNSV 
        TDRSPLTPE TYDAANQAQ QEDPAPPPQ TQADPGGGA 
        GGASQAAAP AAPGGGTGG SGGSGGNGG DGDDGGGLK 
        LPDLKGLMD LLGWLWDKL GHLFNFLKN PYLYLVPMV 
        LFFLLGFLA VILLFPWWI PALFLFASA KTAQKKNVA 
        FYKHVHKPV HHPDGWFWN HETKTWQNL AEIVHHHHH 
        HRRSDGDGD SGEDNYKEI TDAIENFSR KYESSSQSW 
        KWRRRVRSP SQIGETIKQ VTSQLMEAN ATSPDIVTL 
        DTRTPPKIK QHAKKKPQK PETLENSHA GVAIPVNED 
        EINLEETTK KFFYKIEKP TTNSGLSTW ILLSGQTTA 
        KPSRKPVVK PNESQNVTV VVDSNKIIK PLFKKRGTT 
        TSTTTLAPT TTKLTKVKA SVLSGAKSS TTTTTTMTT 
        PTTSSTTTT TNSSTLPIE AKNSETDLS EKKTRRPST 
        TKRKKNKNR RRRPSEKTD SSTKIDKPI QSKEKPIGT 
        QLYNYLSRE VMPTVGVGL VGLMVTAGL AGYFLYPFG 
        VARRSYDID RRDKDNHFY YSDEYSGGI AEEEAIGKV 
        IAGMPSNSL YGSNFKTPT SRHSFNNHR VIEQSTVGG 
        EGATPAAVP EHGPRKRRQ VNDGENELD GSITTDDGP 
        STTTTTTST THRPDKVKS LVDMFKELF HLKINLGLQ 
        LLQNATQAV SKYVSHVQK RLDEHYRNY TLTNSTN
      CDS sequence
      • >TC007806 RA
        atgggtacc gctatgccg agcgcaccg gccgctcag 
        gggttcctg ccaacagta gttcttcag atgttacag 
        tgcccacag tgtcttaaa ctcgaccag tcgttttta 
        aaacgtgaa aatgcagtg cccaagacc tttagtgtt 
        agtgttagt gacatcggc tgtgatagc cgcaactcc 
        ggtaccatc tggaaccac cacaacagc aagcccact 
        tttcgcggc gaaaatatg cgcaaaatt aaacgaaaa 
        gaccaacaa ctactcaca aactttgtc aaaaataat 
        ttggaagat ctaaacacc cctaacaaa acgatcaaa 
        gttggtacc aaaaacaag gttcgtgtt ggtaccacc 
        actcggaaa accacgaaa cttacccca aaccctata 
        gttattaac attactgca ccccccaaa aaaccgaca 
        aaggtggta cgcgtaacc aaaccccct ttcaaaatc 
        aaactaacc ccaaccacc aaaaaaccc aaaataaaa 
        ccaacaatt agacgcgtt gtcacaaaa tggcccaat 
        gaagtcaaa cagaattgg tacgaacaa ggggtacca 
        taccccacc cccaacccc gaaatcaac tcacactcg 
        cccccttac tccctcagc gaggtcgct tcagaccct 
        ggccaggtc tcagaaccc ccccagact gacctaagc 
        aatgctatc agtaccttt aatctggac atggccccc 
        gacccctcc aacatccga gatggggcc gggggttcc 
        ccttgcccc acagtccac atttcaagc tcggtccta 
        accccccaa caacgccaa gactgttcc gacttaaac 
        ctcgtcata aactcccat ttccatcaa aattcagta 
        acagaccgg tcccccctt acccccgaa acgtacgac 
        gcagccaac caggcccag caggaagac ccggcaccg 
        cccccgcaa actcaagcc gatcctggg ggcggcgcc 
        ggcggcgcc tcacaagcc gccgctccc gcggccccc 
        ggtggtggt accggaggg agcggcggt agtggcggt 
        aatgggggc gatggcgat gatggaggt ggtctcaaa 
        ttaccagat ttgaaaggt ttgatggat ttgttgggc 
        tggttgtgg gacaagttg ggccacctc ttcaatttt 
        ttgaaaaat ccctacttg tatctggta ccaatggta 
        ctgtttttc ttgctgggt ttcttggcg gtcattttg 
        ttgttcccc tggtggatc ccggcgttg tttttgttc 
        gccagtgcg aaaacagca cagaaaaaa aatgtcgcg 
        ttttataaa catgtccat aaaccggtg catcaccca 
        gatgggtgg ttttggaac catgagact aaaacctgg 
        cagaatttg gccgaaatc gtgcatcat catcatcat 
        catcgaagg tcggatggt gatggtgac agtggtgaa 
        gataattat aaagaaata acagatgct atagaaaat 
        ttctcgcgg aaatatgag agtagtagt cagtcgtgg 
        aagtggcgg agaagggtc agatctccg agccaaatt 
        ggagaaaca ataaagcaa gtgactagt cagctaatg 
        gaagcaaat gcaacctca cccgatata gtcacactc 
        gatacacga acaccgccc aaaatcaaa cagcacgcg 
        aaaaagaag ccacaaaaa ccagaaacc ctcgaaaat 
        tcacatgca ggggttgcc ataccggtc aacgaagac 
        gagataaat ctcgaagaa acgaccaaa aaattcttc 
        tacaagatt gaaaaaccc acaactaac agtggccta 
        tcgacttgg atcctgtta agcgggcag acaacagcc 
        aaaccgtcc cgaaaaccg gtcgtgaaa ccaaatgag 
        agccaaaat gtcacggtt gtggtggat tctaacaaa 
        atcatcaag cctttgttc aaaaaacgt ggtaccact 
        actagtacc acaacactg gccccaact accaccaaa 
        ctgaccaag gtgaaggct tcagtcttg tcgggtgcc 
        aagtcaagt accaccacc acaacaacc atgacaaca 
        ccaacaact agtagtacc accaccaca actaactcc 
        agtacgtta ccaattgaa gcaaaaaat tccgagact 
        gatttaagt gagaagaaa acgcgccga ccttccacc 
        accaaaagg aagaagaat aagaaccgt cgcagacgc 
        cctagcgaa aaaaccgac agtagtacc aaaattgac 
        aaacccatc cagtccaaa gaaaaaccc atcggaacc 
        caactttac aattacttg tctcgggaa gtgatgccc 
        acggttggg gtcggtctc gtgggcttg atggtcact 
        gctggactt gctgggtac tttttgtac ccatttgga 
        gtcgctcgc aggtcgtac gacatcgac agacgggac 
        aaagacaac cacttttac tactcggat gagtactcg 
        ggtgggatc gctgaggag gaagccatc gggaaagtg 
        atagcggga atgccgtcc aactctttg tacggaagc 
        aacttcaaa acgcccact tccagacac agttttaac 
        aaccatagg gtaattgag caaagtact gttggtgga 
        gagggggca actccggct gcggtaccc gaacacggg 
        cccagaaaa cgcagacaa gttaacgat ggagagaat 
        gaacttgat ggtagtatt accacggat gatggtccc 
        agtactacc accaccaca accagtacc acgcacagg 
        ccggacaaa gtcaagtct ttggtggac atgttcaag 
        gagttgttc cacttgaag ataaacctt ggactccag 
        ttgctacaa aacgcaact caggccgtc tcaaagtac 
        gtttctcac gtgcaaaaa cgcctggac gagcattac 
        cgcaactac acccttacc aatagtacc aactga
      mRNA sequence
      • >TC007806 RA
        tttcaggta ccagccatg ggtaccgct atgccgagc 
        gcaccggcc gctcagggg ttcctgcca acagtagtt 
        cttcagatg ttacagtgc ccacagtgt cttaaactc 
        gaccagtcg tttttaaaa cgtgaaaat gcagtgccc 
        aagaccttt agtgttagt gttagtgac atcggctgt 
        gatagccgc aactccggt accatctgg aaccaccac 
        aacagcaag cccactttt cgcggcgaa aatatgcgc 
        aaaattaaa cgaaaagac caacaacta ctcacaaac 
        tttgtcaaa aataatttg gaagatcta aacacccct 
        aacaaaacg atcaaagtt ggtaccaaa aacaaggtt 
        cgtgttggt accaccact cggaaaacc acgaaactt 
        accccaaac cctatagtt attaacatt actgcaccc 
        cccaaaaaa ccgacaaag gtggtacgc gtaaccaaa 
        ccccctttc aaaatcaaa ctaacccca accaccaaa 
        aaacccaaa ataaaacca acaattaga cgcgttgtc 
        acaaaatgg cccaatgaa gtcaaacag aattggtac 
        gaacaaggg gtaccatac cccaccccc aaccccgaa 
        atcaactca cactcgccc ccttactcc ctcagcgag 
        gtcgcttca gaccctggc caggtctca gaacccccc 
        cagactgac ctaagcaat gctatcagt acctttaat 
        ctggacatg gcccccgac ccctccaac atccgagat 
        ggggccggg ggttcccct tgccccaca gtccacatt 
        tcaagctcg gtcctaacc ccccaacaa cgccaagac 
        tgttccgac ttaaacctc gtcataaac tcccatttc 
        catcaaaat tcagtaaca gaccggtcc ccccttacc 
        cccgaaacg tacgacgca gccaaccag gcccagcag 
        gaagacccg gcaccgccc ccgcaaact caagccgat 
        cctgggggc ggcgccggc ggcgcctca caagccgcc 
        gctcccgcg gcccccggt ggtggtacc ggagggagc 
        ggcggtagt ggcggtaat gggggcgat ggcgatgat 
        ggaggtggt ctcaaatta ccagatttg aaaggtttg 
        atggatttg ttgggctgg ttgtgggac aagttgggc 
        cacctcttc aattttttg aaaaatccc tacttgtat 
        ctggtacca atggtactg tttttcttg ctgggtttc 
        ttggcggtc attttgttg ttcccctgg tggatcccg 
        gcgttgttt ttgttcgcc agtgcgaaa acagcacag 
        aaaaaaaat gtcgcgttt tataaacat gtccataaa 
        ccggtgcat cacccagat gggtggttt tggaaccat 
        gagactaaa acctggcag aatttggcc gaaatcgtg 
        catcatcat catcatcat cgaaggtcg gatggtgat 
        ggtgacagt ggtgaagat aattataaa gaaataaca 
        gatgctata gaaaatttc tcgcggaaa tatgagagt 
        agtagtcag tcgtggaag tggcggaga agggtcaga 
        tctccgagc caaattgga gaaacaata aagcaagtg 
        actagtcag ctaatggaa gcaaatgca acctcaccc 
        gatatagtc acactcgat acacgaaca ccgcccaaa 
        atcaaacag cacgcgaaa aagaagcca caaaaacca 
        gaaaccctc gaaaattca catgcaggg gttgccata 
        ccggtcaac gaagacgag ataaatctc gaagaaacg 
        accaaaaaa ttcttctac aagattgaa aaacccaca 
        actaacagt ggcctatcg acttggatc ctgttaagc 
        gggcagaca acagccaaa ccgtcccga aaaccggtc 
        gtgaaacca aatgagagc caaaatgtc acggttgtg 
        gtggattct aacaaaatc atcaagcct ttgttcaaa 
        aaacgtggt accactact agtaccaca acactggcc 
        ccaactacc accaaactg accaaggtg aaggcttca 
        gtcttgtcg ggtgccaag tcaagtacc accaccaca 
        acaaccatg acaacacca acaactagt agtaccacc 
        accacaact aactccagt acgttacca attgaagca 
        aaaaattcc gagactgat ttaagtgag aagaaaacg 
        cgccgacct tccaccacc aaaaggaag aagaataag 
        aaccgtcgc agacgccct agcgaaaaa accgacagt 
        agtaccaaa attgacaaa cccatccag tccaaagaa 
        aaacccatc ggaacccaa ctttacaat tacttgtct 
        cgggaagtg atgcccacg gttggggtc ggtctcgtg 
        ggcttgatg gtcactgct ggacttgct gggtacttt 
        ttgtaccca tttggagtc gctcgcagg tcgtacgac 
        atcgacaga cgggacaaa gacaaccac ttttactac 
        tcggatgag tactcgggt gggatcgct gaggaggaa 
        gccatcggg aaagtgata gcgggaatg ccgtccaac 
        tctttgtac ggaagcaac ttcaaaacg cccacttcc 
        agacacagt tttaacaac catagggta attgagcaa 
        agtactgtt ggtggagag ggggcaact ccggctgcg 
        gtacccgaa cacgggccc agaaaacgc agacaagtt 
        aacgatgga gagaatgaa cttgatggt agtattacc 
        acggatgat ggtcccagt actaccacc accacaacc 
        agtaccacg cacaggccg gacaaagtc aagtctttg 
        gtggacatg ttcaaggag ttgttccac ttgaagata 
        aaccttgga ctccagttg ctacaaaac gcaactcag 
        gccgtctca aagtacgtt tctcacgtg caaaaacgc 
        ctggacgag cattaccgc aactacacc cttaccaat 
        agtaccaac tgattattt atgtgaagc tagtccagg 
        aggtaatgt ttttggtac cagacaaag tcactacct 
        cctggacta tcaggattt atgtactcc cctgtctac 
        acttaacag tattcttga gagtacgta gattagttt 
        actggggca ggcgtcggg aataaacca taaaaacca 
        aa