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Gene information
General information
TC007790 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 9470..11314
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007790
    iBeetle screen iB_01264
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007790 RA
      MGLNSVTQT VLFTSLVTA VTVVGSYLY QKWKQVKIP 
      SNWERIGSV KKLHLYPLK SGHRVELQR AEVTEVGLR 
      QTKEDEKIF QLRDRVLVV YGAKDHEFR TARTYPKMV 
      LIDVSVHDE DHLAIDAPT MRTLYVKIP NRKDNAVAK 
      VKCWNNEHI EAIDCGDEA AGWFSRYII ERDFGLRLG 
      YNDASHRRD ITKTHHKLL NYYKNLSNH STGLFADLS 
      SVTLINQHS VHDLNKRVG NNSNVTVEN FRHNIIVDG 
      PDLQPYDED NWDWIKVGD NVILRNVKD CTRCVFTTI 
      SSENGIRDI NRQPLKTLE TYRKHSGPD HGPRFGTNL 
      EVAKTGFIN VGDPVFIAR KEETQ
    CDS sequence
    • >TC007790 RA
      atgggtctc aacagtgtg acacaaacc gtccttttt 
      acaagtctt gtgactgct gttactgtc gttggttcc 
      tacttgtat caaaaatgg aaacaggtc aaaatacct 
      tcgaattgg gaacgcata ggaagcgtg aaaaaactt 
      cacttgtat ccacttaaa agtggacac agagtcgaa 
      ttacaaagg gctgaagta acagaagtt ggacttaga 
      caaactaaa gaggacgaa aagatcttt cagttaaga 
      gacagggtt ttggtagtt tatggagct aaggaccat 
      gaatttcgc actgctcgg acctaccca aaaatggtc 
      ctcattgat gtttcggtc cacgatgag gatcacctt 
      gccatagat gctcccact atgaggaca ctctacgtt 
      aaaataccc aaccgaaaa gacaacgcc gttgctaaa 
      gtcaagtgt tggaacaac gaacatatt gaagcaatt 
      gattgtgga gatgaggca gctggctgg ttttcccga 
      tacataatt gagcgagat tttggtcta agactaggt 
      tacaacgat gccagtcat cgacgcgat attacaaaa 
      acccaccac aaactcctc aactactac aagaactta 
      agcaaccat tcaactggc ctgtttgca gacttgtcg 
      agtgttact ctcatcaat caacattca gttcacgat 
      ttgaataaa cgagttggt aacaattcc aatgttact 
      gttgaaaat ttcagacac aacattatt gttgatgga 
      cctgatttg caaccttat gatgaagac aattgggat 
      tggatcaaa gtcggcgat aatgttatt ttgagaaat 
      gttaaagac tgtactagg tgtgtcttt acaaccatt 
      tcgtctgaa aacggaatc agggatata aataggcaa 
      ccgttgaaa actttggaa acatatcgc aaacattca 
      ggacctgat catggacca agattcgga acgaattta 
      gaagttgca aaaactgga tttattaat gttggcgac 
      cctgtattt attgccagg aaggaagaa acgcaataa
    mRNA sequence
    • >TC007790 RA
      tttttctaa acattatca catgtttaa gttttctcg 
      cgttgaaac gacttgaaa cggtaagaa ttaagaaaa 
      tatttattt atacacaga aagttcaca attggtcac 
      cattttatc tcgtagctt atcatataa ttcaagata 
      atttttgta tttgtacaa gtttttatg attaatttg 
      acccccgtc gaagtcgca cgacattgt gtgctctag 
      taattccct aattgttat ttgatgaca ctttttgtc 
      tgaaaatgg gtctcaaca gtgtgacac aaaccgtcc 
      tttttacaa gtcttgtga ctgctgtta ctgtcgttg 
      gttcctact tgtatcaaa aatggaaac aggtcaaaa 
      taccttcga attgggaac gcataggaa gcgtgaaaa 
      aacttcact tgtatccac ttaaaagtg gacacagag 
      tcgaattac aaagggctg aagtaacag aagttggac 
      ttagacaaa ctaaagagg acgaaaaga tctttcagt 
      taagagaca gggttttgg tagtttatg gagctaagg 
      accatgaat ttcgcactg ctcggacct acccaaaaa 
      tggtcctca ttgatgttt cggtccacg atgaggatc 
      accttgcca tagatgctc ccactatga ggacactct 
      acgttaaaa tacccaacc gaaaagaca acgccgttg 
      ctaaagtca agtgttgga acaacgaac atattgaag 
      caattgatt gtggagatg aggcagctg gctggtttt 
      cccgataca taattgagc gagattttg gtctaagac 
      taggttaca acgatgcca gtcatcgac gcgatatta 
      caaaaaccc accacaaac tcctcaact actacaaga 
      acttaagca accattcaa ctggcctgt ttgcagact 
      tgtcgagtg ttactctca tcaatcaac attcagttc 
      acgatttga ataaacgag ttggtaaca attccaatg 
      ttactgttg aaaatttca gacacaaca ttattgttg 
      atggacctg atttgcaac cttatgatg aagacaatt 
      gggattgga tcaaagtcg gcgataatg ttattttga 
      gaaatgtta aagactgta ctaggtgtg tctttacaa 
      ccatttcgt ctgaaaacg gaatcaggg atataaata 
      ggcaaccgt tgaaaactt tggaaacat atcgcaaac 
      attcaggac ctgatcatg gaccaagat tcggaacga 
      atttagaag ttgcaaaaa ctggattta ttaatgttg 
      gcgaccctg tatttattg ccaggaagg aagaaacgc 
      aataaagaa cgttttagg tctatgtgt agagggggg 
      acattacat agttttcat tcattgaga ttgttactt 
      atactatta ttgttatta cttaggcca tcctctgta 
      tataatacg aattaaaat tgactgttc aaaataatt 
      tttttgtgc gtcagggat gataaaaac gt