Status
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Gene information
General information
TC007789 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12628..13784
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007789
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01263

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007789 RA
      MKDLSTLSD TELLEFFNS FDTVLSDVN GVLWNILES 
      IPGASDGIK SLKKIGKQL AVVSNNTTE SLDSFHKQL 
      NSSGFDLRK EEIILPTQA MIAYLKSKN FTNSIFILG 
      MPAMKEAFK EAGFKVANN ENWTKVNSL QEFGLVTNI 
      ASEIGAIIA DIDLNLDFV NLQKSVNLL KRPEVIFLV 
      GATNVAVPL GLDRVMLGP GCYLRILEE ASGRKGLQM 
      AKPNLSLNN YIIQKYGIK DASKVLFIG DSVLADMGF 
      ATKCGYKKL LVLSGLTKK EDLDEWKYE EEYKPEFYV 
      NSLKVVHEL IEKIKQSV
    CDS sequence
    • >TC007789 RA
      atgaaagac ttaagcaca ctgagcgac actgaattg 
      ttagagttt ttcaattct tttgataca gttctttct 
      gatgtgaac ggtgttttg tggaatatt ttggagagt 
      attcctggc gcttccgat ggcataaaa tcgttgaaa 
      aaaatcgga aaacaactc gctgttgta tcaaacaat 
      acaaccgaa tctctcgat tcgttccac aagcaactg 
      aattcttca ggttttgat ttgagaaaa gaggaaatt 
      attttacca actcaagcc atgattgcg tacttaaaa 
      tctaaaaat ttcaccaac agtattttc atcctaggc 
      atgccagcg atgaaagag gcgttcaaa gaggcaggt 
      tttaaagtc gcaaacaac gaaaattgg acaaaagtc 
      aattctctt caagaattt ggacttgtc actaacatt 
      gccagtgaa atcggtgca attattgcc gatattgac 
      cttaatctt gatttcgtc aatctccaa aaatcggtc 
      aatttatta aaacgccct gaagtcatt ttcctagta 
      ggtgccaca aatgtggct gttccttta ggtttagat 
      cgggttatg ttaggccca ggatgttat ttaaggatt 
      cttgaggag gcttcggga cgcaaagga cttcaaatg 
      gccaaaccc aacctcagt ttaaataat tacattatt 
      caaaagtat ggaatcaaa gatgcgtcc aaagtcttg 
      tttatcgga gattcagtg ttggcagat atgggcttt 
      gcgacgaaa tgtggttat aaaaaatta cttgtcttg 
      agtggttta accaaaaaa gaggacttg gacgagtgg 
      aagtatgaa gaggagtat aaaccggag ttttacgtc 
      aacagtttg aaagttgtt cacgaatta attgagaaa 
      ataaagcaa tcagtttaa
    mRNA sequence
    • >TC007789 RA
      gcttcgtaa attaaacaa atttaaaaa aaaatgcga 
      taagacaat tattaaatt ataaaaact atcattttg 
      ctttataat catctttta accatgaaa gacttaagc 
      acactgagc gacactgaa ttgttagag tttttcaat 
      tcttttgat acagttctt tctgatgtg aacggtgtt 
      ttgtggaat attttggag agtattcct ggcgcttcc 
      gatggcata aaatcgttg aaaaaaatc ggaaaacaa 
      ctcgctgtt gtatcaaac aatacaacc gaatctctc 
      gattcgttc cacaagcaa ctgaattct tcaggtttt 
      gatttgaga aaagaggaa attatttta ccaactcaa 
      gccatgatt gcgtactta aaatctaaa aatttcacc 
      aacagtatt ttcatccta ggcatgcca gcgatgaaa 
      gaggcgttc aaagaggca ggttttaaa gtcgcaaac 
      aacgaaaat tggacaaaa gtcaattct cttcaagaa 
      tttggactt gtcactaac attgccagt gaaatcggt 
      gcaattatt gccgatatt gaccttaat cttgatttc 
      gtcaatctc caaaaatcg gtcaattta ttaaaacgc 
      cctgaagtc attttccta gtaggtgcc acaaatgtg 
      gctgttcct ttaggttta gatcgggtt atgttaggc 
      ccaggatgt tatttaagg attcttgag gaggcttcg 
      ggacgcaaa ggacttcaa atggccaaa cccaacctc 
      agtttaaat aattacatt attcaaaag tatggaatc 
      aaagatgcg tccaaagtc ttgtttatc ggagattca 
      gtgttggca gatatgggc tttgcgacg aaatgtggt 
      tataaaaaa ttacttgtc ttgagtggt ttaaccaaa 
      aaagaggac ttggacgag tggaagtat gaagaggag 
      tataaaccg gagttttac gtcaacagt ttgaaagtt 
      gttcacgaa ttaattgag aaaataaag caatcagtt 
      taaaaaaaa catgttata tacttgtac ctttcaatt 
      aaattattc acaacact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY