Status
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Gene information
General information
TC007776 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 225683..228267
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007776
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01262

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Fertility
      mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 2
      ovary without detectable defects - Number of animals showing the phenotype: 8
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      Fertility
      egg shell potentially surface irregular -

      Comment: eggs are not covered with flour as much usual
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007776 RA
        MGLRKLILV SSIILLLNV PHIASDPIR DLALLKHVT 
        ETPINVLVR VFAPLLIPF LLLGLTAIG PLGVIVLTV 
        VNFFAPFLT PYVPVIRDV LKKIVQSID PVLAQLVNI 
        GNLPFEAGP KIVKLLPFV LPLTILLPA FGPIGFGIL 
        WQLLVKNGI IQHLLGNNL SLPTLALSL IILTFLKHL 
        LPNFLPNVG QFIRTVLGI YALLPDFVT PDPITTTAI 
        FGISLVLTP VITLLRSAL APGGGFLNL LFHLTPIPF 
        LLLPQFLPQ LITALGPLV PIALTPILI IVFGPLLLL 
        MGPILLPAL LPFLPGALK PLLLPLLPL LQPILAPLL 
        PLIAPLLTL LNPILPILA PLLLIGPLL LPLFGLLGP 
        LLPLFLAPL LLIIGPILG PLLGPLFAP LLAPLAPLL 
        GLLKPHFAP LMAALGPIA PLVHKLGPF LPILGILGP 
        LLGLLGPVA PLLLTFLGP ILAPVLGPI APILGLAGP 
        LLAVVGPLL STVLGGLGP ILPMILSLL GPLLGPLLG 
        PLAPIFGLL GPFFLLLGP LLGPIAPLI APVLMLIGP 
        VLGPLAPVL GPILGLLGP VLGPLLAIL GPIVPLLLP 
        LLGTLLGPI LGGVGPILG PIFLILGPI LLGLLGPIL 
        GLLGPMLGG LLGPILGLL APVLGPVLS LLGPILGPV 
        LGILKPF
      CDS sequence
      • >TC007776 RA
        atgggttta cgtaaactt attttggtt tcttcaatc 
        attttgttg cttaacgtt cctcatata gcttcggac 
        ccaatacga gacttggct cttcttaaa catgtgacc 
        gaaaccccg ataaatgtg ctagtccgt gtttttgcc 
        cctttgtta atcccattt ttgctactg ggtttgacc 
        gcaattggg ccccttggc gtgatagtt ctaacagtt 
        gtgaatttc tttgctccg tttttgaca ccatacgtg 
        cccgtaata agagacgtt ttgaaaaaa atagtgcag 
        agtattgac ccggtcctt gctcaattg gtaaatatc 
        gggaatctt ccgtttgaa gctggcccg aaaatcgtc 
        aaactttta ccgtttgtg ctaccactt accattctt 
        ttgccggcg tttggacca ataggcttt ggaatttta 
        tggcagttg ttggtgaaa aatggaatt atacaacac 
        ttgttaggt aacaatttg tcattaccc actttggcg 
        ctcagtttg atcatttta acgttcttg aaacattta 
        ctaccgaac tttttgccc aatgttggg cagtttatt 
        aggactgtg ttagggatt tacgctttg ttacccgac 
        tttgtaact cctgatccc atcacgacc actgcgatt 
        ttcggaatt tcgctagtc ctaacacct gttattacc 
        ctgcttagg agtgcgtta gcacctggt ggagggttc 
        ttgaatctt ttattccac cttacacct attccgttc 
        ctacttcta ccacagttc cttccccaa ttgattaca 
        gcactaggt cccctagta cctatagca ctcacaccg 
        atccttata atagttttt ggacctctc cttttacta 
        atgggacca attttactt cctgccctt ttaccattc 
        ttaccaggg gctttaaaa ccactattg ctaccctta 
        ttgccctta ttacaaccg attttagcc ccacttttg 
        cccttaata gccccactt ttgacactt ttaaaccca 
        attttacca atcctggca ccactttta ctaatcggc 
        cctctttta ttaccactt ttcggcctt ttggggcct 
        ttgttaccc ctatttttg gcaccactt ttgctaatt 
        atcggccca attttggga ccattgctt ggaccactc 
        tttgctcct ttgttagct cctttggcc ccacttttg 
        ggactactg aaaccccac ttcgctcct ttaatggca 
        gctttaggt cccattgcg cctttggta cacaaactt 
        gggcccttt ttgcccatt ttgggcatt ttgggccca 
        ttactaggg ctattagga cccgtggca ccgctttta 
        cttaccttc cttgggcca attctagca ccagtacta 
        gggccaatt gcaccaatt ttgggcctg gcaggccca 
        cttttggcg gttgtgggg cctctcctt tcgacagta 
        ttaggtggt ttagggcct attttgccc atgattttg 
        tcccttctt ggtccactt ttagggcca ttgttgggt 
        cccctagcc ccaattttt ggacttttg gggccattt 
        ttcctactt ttgggccca cttcttggc ccgatagct 
        ccactaatt gcccctgtt ttgatgtta attgggccg 
        gtactaggg ccattagca ccagttttg ggtccaatt 
        ttgggactt ttgggacct gttctaggt ccacttctg 
        gctatttta ggaccaatt gtaccactt ttactccca 
        cttcttgga acgttacta gggcctata ttgggcgga 
        gttgggccc attttggga ccgattttc ctaatatta 
        gggccaatt cttttgggg ttgttagga ccaattttg 
        ggtttgttg ggaccaatg ttgggaggt cttttggga 
        cctattttg gggctgcta gccccagta ttggggcca 
        gttttgagc ttattgggc ccaatatta ggcccggtt 
        cttggcatt ttgaaaccg ttttaa
      mRNA sequence
      • >TC007776 RA
        aaacgtatt tttggcacc gccaaaaac ttaaatttg 
        taaaaaata atgtaaaaa tgggtttac gtaaactta 
        ttttggttt cttcaatca ttttgttgc ttaacgttc 
        ctcatatag cttcggacc caatacgag acttggctc 
        ttcttaaac atgtgaccg aaaccccga taaatgtgc 
        tagtccgtg tttttgccc ctttgttaa tcccatttt 
        tgctactgg gtttgaccg caattgggc cccttggcg 
        tgatagttc taacagttg tgaatttct ttgctccgt 
        ttttgacac catacgtgc ccgtaataa gagacgttt 
        tgaaaaaaa tagtgcaga gtattgacc cggtccttg 
        ctcaattgg taaatatcg ggaatcttc cgtttgaag 
        ctggcccga aaatcgtca aacttttac cgtttgtgc 
        taccactta ccattcttt tgccggcgt ttggaccaa 
        taggctttg gaattttat ggcagttgt tggtgaaaa 
        atggaatta tacaacact tgttaggta acaatttgt 
        cattaccca ctttggcgc tcagtttga tcattttaa 
        cgttcttga aacatttac taccgaact ttttgccca 
        atgttgggc agtttatta ggactgtgt tagggattt 
        acgctttgt tacccgact ttgtaactc ctgatccca 
        tcacgacca ctgcgattt tcggaattt cgctagtcc 
        taacacctg ttattaccc tgcttagga gtgcgttag 
        cacctggtg gagggttct tgaatcttt tattccacc 
        ttacaccta ttccgttcc tacttctac cacagttcc 
        ttccccaat tgattacag cactaggtc ccctagtac 
        ctatagcac tcacaccga tccttataa tagtttttg 
        gacctctcc ttttactaa tgggaccaa ttttacttc 
        ctgcccttt taccattct taccagggg ctttaaaac 
        cactattgc tacccttat tgcccttat tacaaccga 
        ttttagccc cacttttgc ccttaatag ccccacttt 
        tgacacttt taaacccaa ttttaccaa tcctggcac 
        cacttttac taatcggcc ctcttttat taccacttt 
        tcggccttt tggggcctt tgttacccc tatttttgg 
        caccacttt tgctaatta tcggcccaa ttttgggac 
        cattgcttg gaccactct ttgctcctt tgttagctc 
        ctttggccc cacttttgg gactactga aaccccact 
        tcgctcctt taatggcag ctttaggtc ccattgcgc 
        ctttggtac acaaacttg ggccctttt tgcccattt 
        tgggcattt tgggcccat tactagggc tattaggac 
        ccgtggcac cgcttttac ttaccttcc ttgggccaa 
        ttctagcac cagtactag ggccaattg caccaattt 
        tgggcctgg caggcccac ttttggcgg ttgtggggc 
        ctctccttt cgacagtat taggtggtt tagggccta 
        ttttgccca tgattttgt cccttcttg gtccacttt 
        tagggccat tgttgggtc ccctagccc caatttttg 
        gacttttgg ggccatttt tcctacttt tgggcccac 
        ttcttggcc cgatagctc cactaattg cccctgttt 
        tgatgttaa ttgggccgg tactagggc cattagcac 
        cagttttgg gtccaattt tgggacttt tgggacctg 
        ttctaggtc cacttctgg ctattttag gaccaattg 
        taccacttt tactcccac ttcttggaa cgttactag 
        ggcctatat tgggcggag ttgggccca ttttgggac 
        cgattttcc taatattag ggccaattc ttttggggt 
        tgttaggac caattttgg gtttgttgg gaccaatgt 
        tgggaggtc ttttgggac ctattttgg ggctgctag 
        ccccagtat tggggccag ttttgagct tattgggcc 
        caatattag gcccggttc ttggcattt tgaaaccgt 
        tttaagtgg gtaatttaa aattgttac ttttagtta 
        gagatagtc ttgtattgt aattacgtg agcaataaa 
        aatgttgaa gaaacttgt tgttttgac ttacctgaa 
        caggcttgt ggtggtaga gggaaaatg agggtagtt 
        gaagacttc cttctggcc actgcacat cccagtgat 
        tgtggcttg aaagcccta agttgctgc atgaagtcg 
        gagcactcc atgagagca acgtcagcg aagtgtaag 
        tcgcagagt atttggttt ttgaagcgt cttgctcgc 
        ttctggttc ggacccgtc aaattgagt ccgcacgaa 
        ttgcatcgc aaacattcc tcgtggtag tgattattg 
        tcgtacagc ggcgaaggt tctggaata ttcaaaaat 
        aatcaaaca attttattc gatcaaatc tcgaaggga 
        tcgtttcta ctgctgtat ctgaaaggt tttttgttg 
        catcgatcc gtctggata ttaaacaag ggaaagtta 
        aataaaacg ttacaaatt aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY