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Gene information
General information
TC007776 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 225683..228267
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC007776
      iBeetle screen iB_01262

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Fertility
      mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 2
      ovary without detectable defects - Number of animals showing the phenotype: 8
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      Fertility
      egg shell potentially surface irregular -

      Comment: eggs are not covered with flour as much usual
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007776 RA
        MGLRKLILV SSIILLLNV PHIASDPIR DLALLKHVT 
ETPINVLVR VFAPLLIPF LLLGLTAIG PLGVIVLTV 
VNFFAPFLT PYVPVIRDV LKKIVQSID PVLAQLVNI 
GNLPFEAGP KIVKLLPFV LPLTILLPA FGPIGFGIL 
WQLLVKNGI IQHLLGNNL SLPTLALSL IILTFLKHL 
LPNFLPNVG QFIRTVLGI YALLPDFVT PDPITTTAI 
FGISLVLTP VITLLRSAL APGGGFLNL LFHLTPIPF 
LLLPQFLPQ LITALGPLV PIALTPILI IVFGPLLLL 
MGPILLPAL LPFLPGALK PLLLPLLPL LQPILAPLL 
PLIAPLLTL LNPILPILA PLLLIGPLL LPLFGLLGP 
LLPLFLAPL LLIIGPILG PLLGPLFAP LLAPLAPLL 
GLLKPHFAP LMAALGPIA PLVHKLGPF LPILGILGP 
LLGLLGPVA PLLLTFLGP ILAPVLGPI APILGLAGP 
LLAVVGPLL STVLGGLGP ILPMILSLL GPLLGPLLG 
PLAPIFGLL GPFFLLLGP LLGPIAPLI APVLMLIGP 
VLGPLAPVL GPILGLLGP VLGPLLAIL GPIVPLLLP 
LLGTLLGPI LGGVGPILG PIFLILGPI LLGLLGPIL 
GLLGPMLGG LLGPILGLL APVLGPVLS LLGPILGPV 
LGILKPF
      CDS sequence
      • >TC007776 RA
        atgggttta cgtaaactt attttggtt tcttcaatc 
attttgttg cttaacgtt cctcatata gcttcggac 
ccaatacga gacttggct cttcttaaa catgtgacc 
gaaaccccg ataaatgtg ctagtccgt gtttttgcc 
cctttgtta atcccattt ttgctactg ggtttgacc 
gcaattggg ccccttggc gtgatagtt ctaacagtt 
gtgaatttc tttgctccg tttttgaca ccatacgtg 
cccgtaata agagacgtt ttgaaaaaa atagtgcag 
agtattgac ccggtcctt gctcaattg gtaaatatc 
gggaatctt ccgtttgaa gctggcccg aaaatcgtc 
aaactttta ccgtttgtg ctaccactt accattctt 
ttgccggcg tttggacca ataggcttt ggaatttta 
tggcagttg ttggtgaaa aatggaatt atacaacac 
ttgttaggt aacaatttg tcattaccc actttggcg 
ctcagtttg atcatttta acgttcttg aaacattta 
ctaccgaac tttttgccc aatgttggg cagtttatt 
aggactgtg ttagggatt tacgctttg ttacccgac 
tttgtaact cctgatccc atcacgacc actgcgatt 
ttcggaatt tcgctagtc ctaacacct gttattacc 
ctgcttagg agtgcgtta gcacctggt ggagggttc 
ttgaatctt ttattccac cttacacct attccgttc 
ctacttcta ccacagttc cttccccaa ttgattaca 
gcactaggt cccctagta cctatagca ctcacaccg 
atccttata atagttttt ggacctctc cttttacta 
atgggacca attttactt cctgccctt ttaccattc 
ttaccaggg gctttaaaa ccactattg ctaccctta 
ttgccctta ttacaaccg attttagcc ccacttttg 
cccttaata gccccactt ttgacactt ttaaaccca 
attttacca atcctggca ccactttta ctaatcggc 
cctctttta ttaccactt ttcggcctt ttggggcct 
ttgttaccc ctatttttg gcaccactt ttgctaatt 
atcggccca attttggga ccattgctt ggaccactc 
tttgctcct ttgttagct cctttggcc ccacttttg 
ggactactg aaaccccac ttcgctcct ttaatggca 
gctttaggt cccattgcg cctttggta cacaaactt 
gggcccttt ttgcccatt ttgggcatt ttgggccca 
ttactaggg ctattagga cccgtggca ccgctttta 
cttaccttc cttgggcca attctagca ccagtacta 
gggccaatt gcaccaatt ttgggcctg gcaggccca 
cttttggcg gttgtgggg cctctcctt tcgacagta 
ttaggtggt ttagggcct attttgccc atgattttg 
tcccttctt ggtccactt ttagggcca ttgttgggt 
cccctagcc ccaattttt ggacttttg gggccattt 
ttcctactt ttgggccca cttcttggc ccgatagct 
ccactaatt gcccctgtt ttgatgtta attgggccg 
gtactaggg ccattagca ccagttttg ggtccaatt 
ttgggactt ttgggacct gttctaggt ccacttctg 
gctatttta ggaccaatt gtaccactt ttactccca 
cttcttgga acgttacta gggcctata ttgggcgga 
gttgggccc attttggga ccgattttc ctaatatta 
gggccaatt cttttgggg ttgttagga ccaattttg 
ggtttgttg ggaccaatg ttgggaggt cttttggga 
cctattttg gggctgcta gccccagta ttggggcca 
gttttgagc ttattgggc ccaatatta ggcccggtt 
cttggcatt ttgaaaccg ttttaa
      mRNA sequence
      • >TC007776 RA
        aaacgtatt tttggcacc gccaaaaac ttaaatttg 
taaaaaata atgtaaaaa tgggtttac gtaaactta 
ttttggttt cttcaatca ttttgttgc ttaacgttc 
ctcatatag cttcggacc caatacgag acttggctc 
ttcttaaac atgtgaccg aaaccccga taaatgtgc 
tagtccgtg tttttgccc ctttgttaa tcccatttt 
tgctactgg gtttgaccg caattgggc cccttggcg 
tgatagttc taacagttg tgaatttct ttgctccgt 
ttttgacac catacgtgc ccgtaataa gagacgttt 
tgaaaaaaa tagtgcaga gtattgacc cggtccttg 
ctcaattgg taaatatcg ggaatcttc cgtttgaag 
ctggcccga aaatcgtca aacttttac cgtttgtgc 
taccactta ccattcttt tgccggcgt ttggaccaa 
taggctttg gaattttat ggcagttgt tggtgaaaa 
atggaatta tacaacact tgttaggta acaatttgt 
cattaccca ctttggcgc tcagtttga tcattttaa 
cgttcttga aacatttac taccgaact ttttgccca 
atgttgggc agtttatta ggactgtgt tagggattt 
acgctttgt tacccgact ttgtaactc ctgatccca 
tcacgacca ctgcgattt tcggaattt cgctagtcc 
taacacctg ttattaccc tgcttagga gtgcgttag 
cacctggtg gagggttct tgaatcttt tattccacc 
ttacaccta ttccgttcc tacttctac cacagttcc 
ttccccaat tgattacag cactaggtc ccctagtac 
ctatagcac tcacaccga tccttataa tagtttttg 
gacctctcc ttttactaa tgggaccaa ttttacttc 
ctgcccttt taccattct taccagggg ctttaaaac 
cactattgc tacccttat tgcccttat tacaaccga 
ttttagccc cacttttgc ccttaatag ccccacttt 
tgacacttt taaacccaa ttttaccaa tcctggcac 
cacttttac taatcggcc ctcttttat taccacttt 
tcggccttt tggggcctt tgttacccc tatttttgg 
caccacttt tgctaatta tcggcccaa ttttgggac 
cattgcttg gaccactct ttgctcctt tgttagctc 
ctttggccc cacttttgg gactactga aaccccact 
tcgctcctt taatggcag ctttaggtc ccattgcgc 
ctttggtac acaaacttg ggccctttt tgcccattt 
tgggcattt tgggcccat tactagggc tattaggac 
ccgtggcac cgcttttac ttaccttcc ttgggccaa 
ttctagcac cagtactag ggccaattg caccaattt 
tgggcctgg caggcccac ttttggcgg ttgtggggc 
ctctccttt cgacagtat taggtggtt tagggccta 
ttttgccca tgattttgt cccttcttg gtccacttt 
tagggccat tgttgggtc ccctagccc caatttttg 
gacttttgg ggccatttt tcctacttt tgggcccac 
ttcttggcc cgatagctc cactaattg cccctgttt 
tgatgttaa ttgggccgg tactagggc cattagcac 
cagttttgg gtccaattt tgggacttt tgggacctg 
ttctaggtc cacttctgg ctattttag gaccaattg 
taccacttt tactcccac ttcttggaa cgttactag 
ggcctatat tgggcggag ttgggccca ttttgggac 
cgattttcc taatattag ggccaattc ttttggggt 
tgttaggac caattttgg gtttgttgg gaccaatgt 
tgggaggtc ttttgggac ctattttgg ggctgctag 
ccccagtat tggggccag ttttgagct tattgggcc 
caatattag gcccggttc ttggcattt tgaaaccgt 
tttaagtgg gtaatttaa aattgttac ttttagtta 
gagatagtc ttgtattgt aattacgtg agcaataaa 
aatgttgaa gaaacttgt tgttttgac ttacctgaa 
caggcttgt ggtggtaga gggaaaatg agggtagtt 
gaagacttc cttctggcc actgcacat cccagtgat 
tgtggcttg aaagcccta agttgctgc atgaagtcg 
gagcactcc atgagagca acgtcagcg aagtgtaag 
tcgcagagt atttggttt ttgaagcgt cttgctcgc 
ttctggttc ggacccgtc aaattgagt ccgcacgaa 
ttgcatcgc aaacattcc tcgtggtag tgattattg 
tcgtacagc ggcgaaggt tctggaata ttcaaaaat 
aatcaaaca attttattc gatcaaatc tcgaaggga 
tcgtttcta ctgctgtat ctgaaaggt tttttgttg 
catcgatcc gtctggata ttaaacaag ggaaagtta 
aataaaacg ttacaaatt aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY