Status
Please wait ...
Gene information
General information
TC007775 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 229492..233127
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007775
      iBeetle screen iB_01261
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      larval musculature ventral thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007775 RA
        MIVTKLLLV LSVCNIFLA SGQTTGLVE KAVLAFPTK 
        LAPLLIPFL LIGLSAIGP LGLLFLVIA PTLAPVTLI 
        LKEVLKGVV QSIDPIVGQ FINFGLLPF EAIPKLLGL 
        LPKILPLLV VLPAIGPIG FLLVARLLL RHEITEHLL 
        GNNLSLPTL IISVIILTL FRHLLPSAV PTVFHFIRI 
        VLGLVALLP AWMPIGPIT LAIIPTVAL ILTPIVTTI 
        RRVLAPKHL LPLVLLLPR ILPALVPIL GPLGPLMIL 
        PIALLLGPV IPALGPLLL PVILPHLPK PVQGLLAPL 
        IPLITPLLG PLAPLQPLI APLTPLLPL LGQLFVPLL 
        LAPVLGFLL PHLNPITIL LLLPALLPH LPPPLKPIV 
        EKFMPKIAP FLGKLTPFK PLLAPLTPL LPLLGHLAP 
        ILLLLPVIM ALGPLLPIV VIILLPHLM PHFPPILHP 
        FLAPLVGVL GPILPLIKP LKPIIDKLG PLLPLLAPF 
        VLPIIIPMI LQVVGMFLP ILAPVLLPE LVPHLPPHI 
        KPLLTPLLP MLAPLLKLL EPLRPALNP FVPLFPLLA 
        HGLVPILLF PVVTFLGPV LPLLLPILL PLLIPHLPT 
        PIKPLLAPL VGLLAPVAK ILAPIEPFL KHLNPLLPL 
        LSQLLVPAL LAPLVEFLG PLLPILLPL LLPALVPML 
        PKPIQALLG PLLPVLQPL VAPFVPLLE ALKPVLMPL 
        LPLLAPLVP ILILGPVFL PILASPIAG PLGPFLLTL 
        LIPVVLPVL GKILGPVAP LIGPLLGPI APILPIILM 
        ILAILGPVL GPLLPVIIT PLLLGPLLP ILGTLLAPL 
        IPILPILLP ILLLASVLI PGVGPVLLP LFVALGPLL 
        ISNFSPILG PHLPIAPLL MLLGPLLLP ILGPRLLPI 
        LAPLLLPIL PILMVLGPA LIPILGTAL GPVLGPMLG 
        PVLGPILRH LKPIFGPLL PILGNLLPM VGPLILPML 
        LPHLLPFLP ILFMVLIGT KIVPKIGPM LAPILVLIG 
        PKIGPILGP EILGHLGPK LGPLLPILL LLPIIGPLL 
        LMLSLLVGP VLLPAILGL IVPRITKII GPIVGLVAP 
        KLAPVLPIL GPLFALAPL LGPLFPILL LIIAPVLFP 
        SLMPVLGPL LAQFAPILV HLPGKILPM VTELGPKLL 
        PILLLMLLL PLLLPLMIT IGPTILPLL KLGH
      CDS sequence
      • >TC007775 RA
        atgattgtt acgaaactt ttgctagtg cttagtgtt 
        tgtaatatc ttcctggca tcaggtcaa acgaccggt 
        ttggtggag aaagctgtc ttggctttt ccaaccaaa 
        ctagcacca ctgttgatt ccctttctt ttgattgga 
        ttatcagct attggacct cttggttta ctgtttcta 
        gtgattgca ccaactctt gcgcctgtg actttaatt 
        ttgaaagaa gttcttaaa ggagttgtg caaagtatt 
        gacccgatt gtgggccag tttatcaac tttgggtta 
        ctgccattt gaggcaatc ccaaaacta ttaggactt 
        ttacctaaa attttgcca ctacttgtg gtattgcca 
        gccattggc ccaattggt tttttgtta gtcgctcgt 
        ttgttacta aggcatgaa attacagaa cacttgttg 
        ggtaacaat ttatcactc ccgacttta ataatttca 
        gtgataatt ttaacactg ttcagacac ttgctacca 
        tcagctgtc ccaacagtg tttcacttt atccgaatt 
        gtgctaggg ttagtggcc ttgttacca gcatggatg 
        cccatcgga cccatcact ttagcaata atcccaaca 
        gtagcacta attttaacc ccaattgtt acaacaatc 
        cgacgagtt ctagcaccc aaacacctc ctacctttg 
        gtactgtta ttacccaga attttacca gcacttgta 
        ccaattttg gggccactt ggacctttg atgatccta 
        cccattgct ttactccta ggacctgta attccagcc 
        ttgggccca cttttactc cccgttatt ttaccccat 
        ctccctaaa ccagtccaa gggttacta gcaccattg 
        ataccttta ataacccca ttattggga ccccttgca 
        cctttgcaa ccactgatc gcacccttg accccgttg 
        ttaccactt ttgggacaa cttttcgtc cctttgtta 
        ctagcccca gttttaggg tttctgtta ccgcacttg 
        aacccgatt acgatttta ttgttacta ccggcactt 
        ttgccccac ttgccccct cccttgaag ccaatcgtg 
        gaaaaattc atgcctaaa atcgcccca tttttgggt 
        aaactaacc ccgtttaaa cccctactg gcaccttta 
        accccgtta ttgcccttg ttgggtcat ctagccccg 
        attttgttg cttctgcct gtgattatg gctctaggg 
        ccgctcctt ccgattgtt gtaattatt ttattgcca 
        catttgatg ccacatttc ccgccgatt ttacacccg 
        tttttggca cctcttgtt ggggtttta ggcccgatt 
        ttaccactg attaaacca ttgaaaccc ataattgat 
        aagttgggc cccctgtta ccacttttg gcaccattc 
        gtacttccc ataattatc ccaatgatt ttgcaagtc 
        gtgggtatg tttttacca attttggca ccggtttta 
        ctaccagag ctggtacca catctacca ccacacatc 
        aaaccactt ttaacgcct ctcttacca atgttggca 
        cccttgtta aaattatta gagccgtta aggcctgcc 
        cttaatccc tttgtgcct ttgtttcct ctcctagcc 
        catggtttg gtgcctatt ttattattc ccagtcgtt 
        acatttttg gggcctgtg ttaccactg ctactacct 
        attttacta cctttgctc ataccacat ttgccaaca 
        ccaataaaa ccattgcta gcgcctcta gttggttta 
        ttggcccca gttgccaaa attcttgca cccattgaa 
        ccatttttg aaacatttg aacccttta ttgccactt 
        ttgagccaa ctgttggtt cctgcatta ctagcccca 
        cttgttgag tttttgggt ccattgtta ccaattttg 
        ctgcctcta cttctaccg gccttggtg ccaatgtta 
        cctaaacct attcaagct ctcttgggg cctttatta 
        ccagtatta cagcctcta gttgcacct tttgtgcct 
        ttgttagag gccttaaaa ccggtctta atgcccttg 
        cttccgctc ttagctcct ttggtacct attctaatt 
        ttaggtccc gtttttttg ccaatacta gcgtctcca 
        attgcgggt cctttagga ccatttctt ttaactctc 
        ttaattccg gtggtattg ccagttttg ggtaaaatt 
        ctagggcct gttgccccg ttaattggg cctttgcta 
        gggcctatt gctccaatt ttgccgatt attttgatg 
        attttggca attttgggg cctgtttta gggccgttg 
        ctcccagtt attatcaca ccgttgttg cttggacct 
        ttgttgcca attctgggg actttattg gcacctctt 
        atcccaatt ctcccgatt ttgttgccg attttatta 
        ctggcatcg gtgttaatt ccaggtgtt ggcccagtt 
        ctccttccc ttatttgtg gctttaggg ccgttgtta 
        atttcaaac ttttcgccg attttaggg ccacatttg 
        cccattgcc ccgctactt atgctacta gggcctttg 
        ttattgcca attttaggg ccacgttta ctgccgatt 
        ttagcaccg cttctttta cccatttta ccaatactt 
        atggtcttg ggacctgcg ttaatccca attttggga 
        acagcttta ggcccagtt ttggggcct atgcttggg 
        cctgtctta gggcccatt ttgcgacat ctcaaacct 
        atttttggg cccttatta ccaatttta ggcaacttg 
        cttccaatg gtggggcct ttaatttta ccaatgttg 
        ctaccgcat ttgttgccc tttttgcct attttgttt 
        atggtccta attggaacc aaaatagtg cctaaaatt 
        ggccctatg ttggcccca attttggta ctgattggg 
        ccaaaaatt ggccctatt ttaggtcca gaaattttg 
        ggtcatttg gggccaaaa cttggacca ctactccct 
        attttgctc cttctaccg attatagga ccgctactc 
        ctaatgtta tccctgctt gtaggtcct gtcctactt 
        ccagctatt ttaggttta atcgttcct cgaataaca 
        aagataata ggtccaatt gtaggtcta gttgcgcca 
        aaattggct cctgtactc ccaatttta ggccctttg 
        tttgcccta gctccactt ttaggacct ctgtttcca 
        attttacta cttattatc gcacctgtt ttattccca 
        tcacttatg ccagtttta ggtccactt ttggcgcaa 
        tttgcaccg attttagtg catttgccg ggaaaaatt 
        ctaccaatg gtaacagaa ttggggcca aaattgcta 
        ccaatttta ctactaatg ttattattg cctctatta 
        cttccctta atgataacg attggtccg acgatactt 
        cccttattg aaactaggc cactga
      mRNA sequence
      • >TC007775 RA
        tccaattca agtggtgct attttaaaa atgtaaagt 
        aaaaattat tgaaaaatg attgttacg aaacttttg 
        ctagtgctt agtgtttgt aatatcttc ctggcatca 
        ggtcaaacg accggtttg gtggagaaa gctgtcttg 
        gcttttcca accaaacta gcaccactg ttgattccc 
        tttcttttg attggatta tcagctatt ggacctctt 
        ggtttactg tttctagtg attgcacca actcttgcg 
        cctgtgact ttaattttg aaagaagtt cttaaagga 
        gttgtgcaa agtattgac ccgattgtg ggccagttt 
        atcaacttt gggttactg ccatttgag gcaatccca 
        aaactatta ggactttta cctaaaatt ttgccacta 
        cttgtggta ttgccagcc attggccca attggtttt 
        ttgttagtc gctcgtttg ttactaagg catgaaatt 
        acagaacac ttgttgggt aacaattta tcactcccg 
        actttaata atttcagtg ataatttta acactgttc 
        agacacttg ctaccatca gctgtccca acagtgttt 
        cactttatc cgaattgtg ctagggtta gtggccttg 
        ttaccagca tggatgccc atcggaccc atcacttta 
        gcaataatc ccaacagta gcactaatt ttaacccca 
        attgttaca acaatccga cgagttcta gcacccaaa 
        cacctccta cctttggta ctgttatta cccagaatt 
        ttaccagca cttgtacca attttgggg ccacttgga 
        cctttgatg atcctaccc attgcttta ctcctagga 
        cctgtaatt ccagccttg ggcccactt ttactcccc 
        gttatttta ccccatctc cctaaacca gtccaaggg 
        ttactagca ccattgata cctttaata accccatta 
        ttgggaccc cttgcacct ttgcaacca ctgatcgca 
        cccttgacc ccgttgtta ccacttttg ggacaactt 
        ttcgtccct ttgttacta gccccagtt ttagggttt 
        ctgttaccg cacttgaac ccgattacg attttattg 
        ttactaccg gcacttttg ccccacttg ccccctccc 
        ttgaagcca atcgtggaa aaattcatg cctaaaatc 
        gccccattt ttgggtaaa ctaaccccg tttaaaccc 
        ctactggca cctttaacc ccgttattg cccttgttg 
        ggtcatcta gccccgatt ttgttgctt ctgcctgtg 
        attatggct ctagggccg ctccttccg attgttgta 
        attatttta ttgccacat ttgatgcca catttcccg 
        ccgatttta cacccgttt ttggcacct cttgttggg 
        gttttaggc ccgatttta ccactgatt aaaccattg 
        aaacccata attgataag ttgggcccc ctgttacca 
        cttttggca ccattcgta cttcccata attatccca 
        atgattttg caagtcgtg ggtatgttt ttaccaatt 
        ttggcaccg gttttacta ccagagctg gtaccacat 
        ctaccacca cacatcaaa ccactttta acgcctctc 
        ttaccaatg ttggcaccc ttgttaaaa ttattagag 
        ccgttaagg cctgccctt aatcccttt gtgcctttg 
        tttcctctc ctagcccat ggtttggtg cctatttta 
        ttattccca gtcgttaca tttttgggg cctgtgtta 
        ccactgcta ctacctatt ttactacct ttgctcata 
        ccacatttg ccaacacca ataaaacca ttgctagcg 
        cctctagtt ggtttattg gccccagtt gccaaaatt 
        cttgcaccc attgaacca tttttgaaa catttgaac 
        cctttattg ccacttttg agccaactg ttggttcct 
        gcattacta gccccactt gttgagttt ttgggtcca 
        ttgttacca attttgctg cctctactt ctaccggcc 
        ttggtgcca atgttacct aaacctatt caagctctc 
        ttggggcct ttattacca gtattacag cctctagtt 
        gcacctttt gtgcctttg ttagaggcc ttaaaaccg 
        gtcttaatg cccttgctt ccgctctta gctcctttg 
        gtacctatt ctaatttta ggtcccgtt tttttgcca 
        atactagcg tctccaatt gcgggtcct ttaggacca 
        tttctttta actctctta attccggtg gtattgcca 
        gttttgggt aaaattcta gggcctgtt gccccgtta 
        attgggcct ttgctaggg cctattgct ccaattttg 
        ccgattatt ttgatgatt ttggcaatt ttggggcct 
        gttttaggg ccgttgctc ccagttatt atcacaccg 
        ttgttgctt ggacctttg ttgccaatt ctggggact 
        ttattggca cctcttatc ccaattctc ccgattttg 
        ttgccgatt ttattactg gcatcggtg ttaattcca 
        ggtgttggc ccagttctc cttccctta tttgtggct 
        ttagggccg ttgttaatt tcaaacttt tcgccgatt 
        ttagggcca catttgccc attgccccg ctacttatg 
        ctactaggg cctttgtta ttgccaatt ttagggcca 
        cgtttactg ccgatttta gcaccgctt cttttaccc 
        attttacca atacttatg gtcttggga cctgcgtta 
        atcccaatt ttgggaaca gctttaggc ccagttttg 
        gggcctatg cttgggcct gtcttaggg cccattttg 
        cgacatctc aaacctatt tttgggccc ttattacca 
        attttaggc aacttgctt ccaatggtg gggccttta 
        attttacca atgttgcta ccgcatttg ttgcccttt 
        ttgcctatt ttgtttatg gtcctaatt ggaaccaaa 
        atagtgcct aaaattggc cctatgttg gccccaatt 
        ttggtactg attgggcca aaaattggc cctatttta 
        ggtccagaa attttgggt catttgggg ccaaaactt 
        ggaccacta ctccctatt ttgctcctt ctaccgatt 
        ataggaccg ctactccta atgttatcc ctgcttgta 
        ggtcctgtc ctacttcca gctatttta ggtttaatc 
        gttcctcga ataacaaag ataataggt ccaattgta 
        ggtctagtt gcgccaaaa ttggctcct gtactccca 
        attttaggc cctttgttt gccctagct ccactttta 
        ggacctctg tttccaatt ttactactt attatcgca 
        cctgtttta ttcccatca cttatgcca gttttaggt 
        ccacttttg gcgcaattt gcaccgatt ttagtgcat 
        ttgccggga aaaattcta ccaatggta acagaattg 
        gggccaaaa ttgctacca attttacta ctaatgtta 
        ttattgcct ctattactt cccttaatg ataacgatt 
        ggtccgacg atacttccc ttattgaaa ctaggccac 
        tgaccctat ttagctgat cgattattt atatgtaca 
        aaaagtatt gtatttttt taatggacg caatgtaac 
        aaataaagt tacgctaaa aat