| General information | |||
| TC007759 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 387597..392537 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007759 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01259 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
remarks: entire repetition development delayed, later than schedule, hours 60
Pupal morphology
remarks: entire repetition development delayed, later than schedule, hours 60
Adult morphology
remarks: entire repetition development delayed, later than schedule, hours 60
- >TC007759 RA
MSTENIIRM EEEESGECN NELSLTRPS LGGSEYDDE LGIPESLII VDVGDGPVV IEHSDEEIE DDVFDGLPF SSIHKPPIK FKSFRRNVF IPSLDISVT IKDYERNLT THMLNPNLY TISLVHGTF NWQIKKRYK QIQHLHQQL ILFRAGLNI PFPSKTHKR KRKSFKMNI LVPYEKLCD RIKQLENYL NNLLSINIY RNHPATIEF LEVSHLSFV QGLGIKGKE GVVKKRTGS TQPGQAGCN CCGLIRCMF CIRCQHLCK DILCARWNE RWFFIKDTC FGYINPDDG MIKCVILFD QGFEVASGL YSIGLQTGF QIQTLSRQI AFKCWTRRK SREWVEALK EVASGTARE FTQANQHRS FAPLRTTIA ACWFVDGAS YMSAVADAI ENAKEEVFI TDWWLSPEI YMKRPAISG DYWRLDKLL QRKAASGVR IFVLLYKEV ELALGLNSY YSKQTLAEL SENIKVLRH PDHARVGVF LWAHHEKMV VVDQSYAFV GGIDLCYGR WDDAKHRLT DLGSITPSI DVSTLKKKT SSVPGGDAI YPIPIPIYK QTPIPVVTE PEAPEPCPL PQLEPGDTL LIPTVKLKC NTPDMERKN VIDTIKNTV KTKGKDLIS LVYTPHEDS VEKTEEEKK PQTQDEFLE TLNGSSKLW VGKDYVNFI VKDFNNLDS PFDDFIDRV TTPRMPWHD VGVCVQGAA ARDVSRHFI QRWNATKLE KAKSNKCYP YLLPKSYDD FKTLPIVFP NEVSNVSCQ VLRSVSTWS CGFLEPDTV EQSIHEAYI DTINKAQHY IYIENQFFI SLPYTNPNT RNQIAEALY KRIVRAFKA KEVFRVFVV MPLLPGFEG EVGGPTGTS LHAITHWNY CSISQGKDA ILSRLQEAG IEDPSDFIS FYGLRNHAT LNCEPITEL IYVHSKLMI VDDKTVICG SANINDRSL IGKRDSEIA VLIEDEAFD DGVMNGKKF PCGKFAGGL RKYLFKEHL GLLGKEHEM IDFDITDPI SDYFYKEVW YKTASLNTE FYEKVFHCI PTDRVKNFA DLKKNQEEK PLYSNEISR AEKMLESIQ GHLVLLPLH FLSQESLTP AASSVEGMM PTSLWT
- >TC007759 RB
MEEEESGEC NNELSLTRP SLGGSEYDD ELGIPESLI IVDVGDGPV VIEHSDEEI EDDVFDGLP FSSIHKPPI KFKSFRRNV FIPSLDISV TIKDYERNL TTHMLNPNL YTISLVHGT FNWQIKKRY KQIQHLHQQ LILFRAGLN IPFPSKTHK RKRKSFKMN ILVPYEKLC DRIKQLENY LNNLLSINI YRNHPATIE FLEVSHLSF VQGLGIKGK EGVVKKRTG STQPGQAGC NCCGLIRCM FCIRCQHLC KDILCARWN ERWFFIKDT CFGYINPDD GMIKCVILF DQGFEVASG LYSIGLQTG FQIQTLSRQ IAFKCWTRR KSREWVEAL KEVASGTAR EFTQANQHR SFAPLRTTI AACWFVDGA SYMSAVADA IENAKEEVF ITDWWLSPE IYMKRPAIS GDYWRLDKL LQRKAASGV RIFVLLYKE VELALGLNS YYSKQTLAE LSENIKVLR HPDHARVGV FLWAHHEKM VVVDQSYAF VGGIDLCYG RWDDAKHRL TDLGSITPS IDVSTLKKK TSSVPGGDA IYPIPIPIY KQTPIPVVT EPEAPEPCP LPQLEPGDT LLIPTVKLK CNTPDMERK NVIDTIKNT VKTKGKDLI SLVYTPHED SVEKTEEEK KPQTQDEFL ETLNGSSKL WVGKDYVNF IVKDFNNLD SPFDDFIDR VTTPRMPWH DVGVCVQGA AARDVSRHF IQRWNATKL EKAKSNKCY PYLLPKSYD DFKTLPIVF PNEVSNVSC QVLRSVSTW SCGFLEPDT VEQSIHEAY IDTINKAQH YIYIENQFF ISLPYTNPN TRNQIAEAL YKRIVRAFK AKEVFRVFV VMPLLPGFE GEVGGPTGT SLHAITHWN YCSISQGKD AILSRLQEA GIEDPSDFI SFYGLRNHA TLNCEPITE LIYVHSKLM IVDDKTVIC GSANINDRS LIGKRDSEI AVLIEDEAF DDGVMNGKK FPCGKFAGG LRKYLFKEH LGLLGKEHE MIDFDITDP ISDYFYKEV WYKTASLNT EFYEKVFHC IPTDRVKNF ADLKKNQEE KPLYSNEIS RAEKMLESI QGHLVLLPL HFLSQESLT PAASSVEGM MPTSLWT
- >TC007759 RA
atgtcaaca gagaatatc attagaatg gaggaagag gaatcaggt gaatgcaac aatgagctg tctttgact agaccgagt ctgggaggc tccgagtat gacgatgag ctgggaatc cccgaatcc ctcattatc gtggatgtg ggggatggc cctgttgtc atagagcac tcagatgag gaaattgaa gatgacgtc ttcgacggt ttgcccttt tcttcaatt cacaaaccg ccaatcaaa ttcaagtct ttccgacga aatgtcttc atcccgtcc cttgatatt tcagtaacc atcaaagac tacgaacgc aacttaaca acgcacatg cttaatcct aacttatat accatttcc ctagtccat ggcactttc aactggcaa atcaaaaaa cgctacaaa caaatccag cacttgcac caacagtta attctattc agggctggt ttaaacatc ccatttccc agcaaaacg cacaaaagg aaacgcaaa agtttcaaa atgaacatt ctggtaccg tatgaaaaa ctatgcgat cggatcaag cagcttgaa aattacctc aataacttg ctctctatt aatatttat cgcaaccat cctgccact atcgaattt ttggaagtt tcgcatcta tcgttcgtc caaggtttg ggtattaaa ggtaaggaa ggagtggtc aaaaaacgg acaggaagc acgcagccg ggacaagcg gggtgcaat tgttgcggg ctcatcagg tgcatgttc tgcataaga tgtcagcac ttgtgcaag gacattttg tgcgcgcgc tggaacgag cgctggttt ttcatcaaa gacacctgt ttcggctac attaaccct gatgatggc atgatcaaa tgcgtcatt ttattcgac caaggtttc gaggtggcg tccggtttg tactccatc ggtttacag accggtttc caaatccaa acattgagt cgccaaatc gcgtttaaa tgttggacg aggaggaaa agtcgggag tgggtggag gcactcaaa gaggtggcc tcggggact gcccgggaa ttcacacaa gccaaccaa caccgctct tttgcacct ttaaggacc acaattgcg gcgtgttgg ttcgtagat ggcgctagt tacatgtcg gcggttgct gacgctatt gaaaatgca aaggaggag gtttttatc actgattgg tggttgagt ccggagatt tatatgaaa cggccagcg attagtggc gattattgg aggttggat aagttgttg caaaggaag gctgcgagt ggtgttcgg atttttgtg ctactttac aaggaagtg gagttggcg ttgggatta aacagttac tatagtaag caaactctc gcggaatta tcggaaaat atcaaagtt ttgcgacac ccggaccat gcccgtgtt ggggttttt ctgtgggca catcatgaa aaaatggtc gttgtggac caaagttac gcgtttgtt ggcggcatt gatttgtgc tatggacgc tgggatgac gccaaacac agattgaca gatcttgga agcataaca ccatcaatt gacgtttca acgttgaaa aagaaaaca tcaagtgtg ccaggaggc gacgcgatt tacccaatc cctatcccg atttacaaa caaacgcca atcccggtt gtaaccgaa ccggaagca cccgaacct tgccccctc cctcaacta gaacctggc gacactctt ttaatacca actgttaaa ctcaaatgt aacactcct gacatggag cgcaaaaac gtaatagac acaatcaaa aacacggtt aaaacaaaa ggaaaagat ctcatctca ttggtttac actcctcat gaagatagc gtcgagaaa acagaggaa gagaaaaaa ccacaaaca caggacgag tttttggaa actttgaac ggatcatcg aaattgtgg gttgggaag gattatgtc aattttatt gttaaggat tttaataat ttggattcg ccgtttgat gattttatc gatcgggtg accacacct cggatgccg tggcatgat gtgggggtt tgtgttcag ggggctgcc gctagagac gtctcgaga cattttatc caacggtgg aatgccacc aagttggag aaagccaag tcgaataaa tgctatcct tatttattg ccgaaaagt tacgacgat tttaaaact ttgccgatt gtgttcccg aacgaagtt agcaacgtt tcatgccag gtcctccga agtgttagt acatggtct tgtggtttt ttagaacca gatacagtc gagcaaagc attcacgag gcctatatt gacactatt aacaaagcc cagcattat atttacata gaaaatcag tttttcatc tcactgccg tacaccaat ccaaacaca aggaaccaa atagccgaa gcgctttac aaacgaatt gtccgagct ttcaaagcc aaagaagtg ttccgtgtc tttgttgtt atgccgctg ttgcctggc tttgagggt gaagttggg gggcccaca ggcacctcc ctgcatgcc ataacacac tggaattat tgctcaatc tcgcaaggt aaagatgcg attttgagt cggttacaa gaggcaggg attgaggat ccgagcgat tttatttcg ttctacggt ttgagaaat catgcgact ttaaactgt gagccaatc actgaattg atttacgtc cactccaag ttaatgata gtcgatgat aaaactgtg atttgtggc tcggctaat attaacgat cgctctcta atcggtaaa agagattcg gaaattgcc gttttgatc gaagatgag gccttcgat gatggcgtg atgaacgga aagaagttc ccatgcgga aaattcgca gggggttta cgtaaatat ttattcaag gaacattta ggtttgttg gggaaggag cacgaaatg atcgatttt gacataacc gatccaatc tcggattat ttctataaa gaagtttgg tacaaaacg gccagtttg aatacagag ttttacgaa aaagtcttc cactgtatt ccaacagac agggtgaaa aatttcgcc gatttgaag aaaaatcaa gaggagaag cccctgtat agcaacgag atttcgcgg gctgaaaaa atgttggag agtattcag ggtcacctt gtactcttg cctttacat ttcctgagt caggaaagc ctgactcct gccgctagt tcagtagaa gggatgatg ccaacgtcc ttgtggact taa
- >TC007759 RB
atggaggaa gaggaatca ggtgaatgc aacaatgag ctgtctttg actagaccg agtctggga ggctccgag tatgacgat gagctggga atccccgaa tccctcatt atcgtggat gtgggggat ggccctgtt gtcatagag cactcagat gaggaaatt gaagatgac gtcttcgac ggtttgccc ttttcttca attcacaaa ccgccaatc aaattcaag tctttccga cgaaatgtc ttcatcccg tcccttgat atttcagta accatcaaa gactacgaa cgcaactta acaacgcac atgcttaat cctaactta tataccatt tccctagtc catggcact ttcaactgg caaatcaaa aaacgctac aaacaaatc cagcacttg caccaacag ttaattcta ttcagggct ggtttaaac atcccattt cccagcaaa acgcacaaa aggaaacgc aaaagtttc aaaatgaac attctggta ccgtatgaa aaactatgc gatcggatc aagcagctt gaaaattac ctcaataac ttgctctct attaatatt tatcgcaac catcctgcc actatcgaa tttttggaa gtttcgcat ctatcgttc gtccaaggt ttgggtatt aaaggtaag gaaggagtg gtcaaaaaa cggacagga agcacgcag ccgggacaa gcggggtgc aattgttgc gggctcatc aggtgcatg ttctgcata agatgtcag cacttgtgc aaggacatt ttgtgcgcg cgctggaac gagcgctgg tttttcatc aaagacacc tgtttcggc tacattaac cctgatgat ggcatgatc aaatgcgtc attttattc gaccaaggt ttcgaggtg gcgtccggt ttgtactcc atcggttta cagaccggt ttccaaatc caaacattg agtcgccaa atcgcgttt aaatgttgg acgaggagg aaaagtcgg gagtgggtg gaggcactc aaagaggtg gcctcgggg actgcccgg gaattcaca caagccaac caacaccgc tcttttgca cctttaagg accacaatt gcggcgtgt tggttcgta gatggcgct agttacatg tcggcggtt gctgacgct attgaaaat gcaaaggag gaggttttt atcactgat tggtggttg agtccggag atttatatg aaacggcca gcgattagt ggcgattat tggaggttg gataagttg ttgcaaagg aaggctgcg agtggtgtt cggattttt gtgctactt tacaaggaa gtggagttg gcgttggga ttaaacagt tactatagt aagcaaact ctcgcggaa ttatcggaa aatatcaaa gttttgcga cacccggac catgcccgt gttggggtt tttctgtgg gcacatcat gaaaaaatg gtcgttgtg gaccaaagt tacgcgttt gttggcggc attgatttg tgctatgga cgctgggat gacgccaaa cacagattg acagatctt ggaagcata acaccatca attgacgtt tcaacgttg aaaaagaaa acatcaagt gtgccagga ggcgacgcg atttaccca atccctatc ccgatttac aaacaaacg ccaatcccg gttgtaacc gaaccggaa gcacccgaa ccttgcccc ctccctcaa ctagaacct ggcgacact cttttaata ccaactgtt aaactcaaa tgtaacact cctgacatg gagcgcaaa aacgtaata gacacaatc aaaaacacg gttaaaaca aaaggaaaa gatctcatc tcattggtt tacactcct catgaagat agcgtcgag aaaacagag gaagagaaa aaaccacaa acacaggac gagtttttg gaaactttg aacggatca tcgaaattg tgggttggg aaggattat gtcaatttt attgttaag gattttaat aatttggat tcgccgttt gatgatttt atcgatcgg gtgaccaca cctcggatg ccgtggcat gatgtgggg gtttgtgtt cagggggct gccgctaga gacgtctcg agacatttt atccaacgg tggaatgcc accaagttg gagaaagcc aagtcgaat aaatgctat ccttattta ttgccgaaa agttacgac gattttaaa actttgccg attgtgttc ccgaacgaa gttagcaac gtttcatgc caggtcctc cgaagtgtt agtacatgg tcttgtggt tttttagaa ccagataca gtcgagcaa agcattcac gaggcctat attgacact attaacaaa gcccagcat tatatttac atagaaaat cagtttttc atctcactg ccgtacacc aatccaaac acaaggaac caaatagcc gaagcgctt tacaaacga attgtccga gctttcaaa gccaaagaa gtgttccgt gtctttgtt gttatgccg ctgttgcct ggctttgag ggtgaagtt ggggggccc acaggcacc tccctgcat gccataaca cactggaat tattgctca atctcgcaa ggtaaagat gcgattttg agtcggtta caagaggca gggattgag gatccgagc gattttatt tcgttctac ggtttgaga aatcatgcg actttaaac tgtgagcca atcactgaa ttgatttac gtccactcc aagttaatg atagtcgat gataaaact gtgatttgt ggctcggct aatattaac gatcgctct ctaatcggt aaaagagat tcggaaatt gccgttttg atcgaagat gaggccttc gatgatggc gtgatgaac ggaaagaag ttcccatgc ggaaaattc gcagggggt ttacgtaaa tatttattc aaggaacat ttaggtttg ttggggaag gagcacgaa atgatcgat tttgacata accgatcca atctcggat tatttctat aaagaagtt tggtacaaa acggccagt ttgaataca gagttttac gaaaaagtc ttccactgt attccaaca gacagggtg aaaaatttc gccgatttg aagaaaaat caagaggag aagcccctg tatagcaac gagatttcg cgggctgaa aaaatgttg gagagtatt cagggtcac cttgtactc ttgccttta catttcctg agtcaggaa agcctgact cctgccgct agttcagta gaagggatg atgccaacg tccttgtgg acttaa
- >TC007759 RA
gatgaatta catcagacc catactatt taattaaac ccagcccca tgtccctgc ttttgtaac cgtttcagt tacgccact tccctccac ccaacaact ccacaaagt aagtcagga aaacttgtt tacaatatt taatttttg gccacaagt gtgtaaata gaggttatg atgataatt tgtttcgtt taaatactt aaaaattgc gatttcatt atttgtgtg cgataagat aagaacata actttgtct attatttgt aaaatgtca acagagaat atcattaga atggaggaa gaggaatca ggtgaatgc aacaatgag ctgtctttg actagaccg agtctggga ggctccgag tatgacgat gagctggga atccccgaa tccctcatt atcgtggat gtgggggat ggccctgtt gtcatagag cactcagat gaggaaatt gaagatgac gtcttcgac ggtttgccc ttttcttca attcacaaa ccgccaatc aaattcaag tctttccga cgaaatgtc ttcatcccg tcccttgat atttcagta accatcaaa gactacgaa cgcaactta acaacgcac atgcttaat cctaactta tataccatt tccctagtc catggcact ttcaactgg caaatcaaa aaacgctac aaacaaatc cagcacttg caccaacag ttaattcta ttcagggct ggtttaaac atcccattt cccagcaaa acgcacaaa aggaaacgc aaaagtttc aaaatgaac attctggta ccgtatgaa aaactatgc gatcggatc aagcagctt gaaaattac ctcaataac ttgctctct attaatatt tatcgcaac catcctgcc actatcgaa tttttggaa gtttcgcat ctatcgttc gtccaaggt ttgggtatt aaaggtaag gaaggagtg gtcaaaaaa cggacagga agcacgcag ccgggacaa gcggggtgc aattgttgc gggctcatc aggtgcatg ttctgcata agatgtcag cacttgtgc aaggacatt ttgtgcgcg cgctggaac gagcgctgg tttttcatc aaagacacc tgtttcggc tacattaac cctgatgat ggcatgatc aaatgcgtc attttattc gaccaaggt ttcgaggtg gcgtccggt ttgtactcc atcggttta cagaccggt ttccaaatc caaacattg agtcgccaa atcgcgttt aaatgttgg acgaggagg aaaagtcgg gagtgggtg gaggcactc aaagaggtg gcctcgggg actgcccgg gaattcaca caagccaac caacaccgc tcttttgca cctttaagg accacaatt gcggcgtgt tggttcgta gatggcgct agttacatg tcggcggtt gctgacgct attgaaaat gcaaaggag gaggttttt atcactgat tggtggttg agtccggag atttatatg aaacggcca gcgattagt ggcgattat tggaggttg gataagttg ttgcaaagg aaggctgcg agtggtgtt cggattttt gtgctactt tacaaggaa gtggagttg gcgttggga ttaaacagt tactatagt aagcaaact ctcgcggaa ttatcggaa aatatcaaa gttttgcga cacccggac catgcccgt gttggggtt tttctgtgg gcacatcat gaaaaaatg gtcgttgtg gaccaaagt tacgcgttt gttggcggc attgatttg tgctatgga cgctgggat gacgccaaa cacagattg acagatctt ggaagcata acaccatca attgacgtt tcaacgttg aaaaagaaa acatcaagt gtgccagga ggcgacgcg atttaccca atccctatc ccgatttac aaacaaacg ccaatcccg gttgtaacc gaaccggaa gcacccgaa ccttgcccc ctccctcaa ctagaacct ggcgacact cttttaata ccaactgtt aaactcaaa tgtaacact cctgacatg gagcgcaaa aacgtaata gacacaatc aaaaacacg gttaaaaca aaaggaaaa gatctcatc tcattggtt tacactcct catgaagat agcgtcgag aaaacagag gaagagaaa aaaccacaa acacaggac gagtttttg gaaactttg aacggatca tcgaaattg tgggttggg aaggattat gtcaatttt attgttaag gattttaat aatttggat tcgccgttt gatgatttt atcgatcgg gtgaccaca cctcggatg ccgtggcat gatgtgggg gtttgtgtt cagggggct gccgctaga gacgtctcg agacatttt atccaacgg tggaatgcc accaagttg gagaaagcc aagtcgaat aaatgctat ccttattta ttgccgaaa agttacgac gattttaaa actttgccg attgtgttc ccgaacgaa gttagcaac gtttcatgc caggtcctc cgaagtgtt agtacatgg tcttgtggt tttttagaa ccagataca gtcgagcaa agcattcac gaggcctat attgacact attaacaaa gcccagcat tatatttac atagaaaat cagtttttc atctcactg ccgtacacc aatccaaac acaaggaac caaatagcc gaagcgctt tacaaacga attgtccga gctttcaaa gccaaagaa gtgttccgt gtctttgtt gttatgccg ctgttgcct ggctttgag ggtgaagtt ggggggccc acaggcacc tccctgcat gccataaca cactggaat tattgctca atctcgcaa ggtaaagat gcgattttg agtcggtta caagaggca gggattgag gatccgagc gattttatt tcgttctac ggtttgaga aatcatgcg actttaaac tgtgagcca atcactgaa ttgatttac gtccactcc aagttaatg atagtcgat gataaaact gtgatttgt ggctcggct aatattaac gatcgctct ctaatcggt aaaagagat tcggaaatt gccgttttg atcgaagat gaggccttc gatgatggc gtgatgaac ggaaagaag ttcccatgc ggaaaattc gcagggggt ttacgtaaa tatttattc aaggaacat ttaggtttg ttggggaag gagcacgaa atgatcgat tttgacata accgatcca atctcggat tatttctat aaagaagtt tggtacaaa acggccagt ttgaataca gagttttac gaaaaagtc ttccactgt attccaaca gacagggtg aaaaatttc gccgatttg aagaaaaat caagaggag aagcccctg tatagcaac gagatttcg cgggctgaa aaaatgttg gagagtatt cagggtcac cttgtactc ttgccttta catttcctg agtcaggaa agcctgact cctgccgct agttcagta gaagggatg atgccaacg tccttgtgg acttaaaac tgatattac gaccgtcca taaacaggt gctacaatt tttagccaa cagcatttc cacaaagtg acttaaatg tgtgcgcaa ataacaaag cttccaaag atattattt attgtgtca gtcgttgtt ttaatttgt tacaacttt tacagtatt atacatttt tagaaacca tgttcgcat ttactctgt ttatgtcag ctaattaca gttatttat ctaagttgg ctcgtttaa atcatttac cttcactgt ttacaaatt accgatcag gtaggtaaa gcaatgttt tctccaaat aaataggat attttata
- >TC007759 RB
gatgaatta catcagacc catactatt taattaaac ccagcccca tgtccctgc ttttgtaac cgtttcagt tacgccact tccctccac ccaacaact ccacaaaaa tggaggaag aggaatcag gtgaatgca acaatgagc tgtctttga ctagaccga gtctgggag gctccgagt atgacgatg agctgggaa tccccgaat ccctcatta tcgtggatg tgggggatg gccctgttg tcatagagc actcagatg aggaaattg aagatgacg tcttcgacg gtttgccct tttcttcaa ttcacaaac cgccaatca aattcaagt ctttccgac gaaatgtct tcatcccgt cccttgata tttcagtaa ccatcaaag actacgaac gcaacttaa caacgcaca tgcttaatc ctaacttat ataccattt ccctagtcc atggcactt tcaactggc aaatcaaaa aacgctaca aacaaatcc agcacttgc accaacagt taattctat tcagggctg gtttaaaca tcccatttc ccagcaaaa cgcacaaaa ggaaacgca aaagtttca aaatgaaca ttctggtac cgtatgaaa aactatgcg atcggatca agcagcttg aaaattacc tcaataact tgctctcta ttaatattt atcgcaacc atcctgcca ctatcgaat ttttggaag tttcgcatc tatcgttcg tccaaggtt tgggtatta aaggtaagg aaggagtgg tcaaaaaac ggacaggaa gcacgcagc cgggacaag cggggtgca attgttgcg ggctcatca ggtgcatgt tctgcataa gatgtcagc acttgtgca aggacattt tgtgcgcgc gctggaacg agcgctggt ttttcatca aagacacct gtttcggct acattaacc ctgatgatg gcatgatca aatgcgtca ttttattcg accaaggtt tcgaggtgg cgtccggtt tgtactcca tcggtttac agaccggtt tccaaatcc aaacattga gtcgccaaa tcgcgttta aatgttgga cgaggagga aaagtcggg agtgggtgg aggcactca aagaggtgg cctcgggga ctgcccggg aattcacac aagccaacc aacaccgct cttttgcac ctttaagga ccacaattg cggcgtgtt ggttcgtag atggcgcta gttacatgt cggcggttg ctgacgcta ttgaaaatg caaaggagg aggttttta tcactgatt ggtggttga gtccggaga tttatatga aacggccag cgattagtg gcgattatt ggaggttgg ataagttgt tgcaaagga aggctgcga gtggtgttc ggatttttg tgctacttt acaaggaag tggagttgg cgttgggat taaacagtt actatagta agcaaactc tcgcggaat tatcggaaa atatcaaag ttttgcgac acccggacc atgcccgtg ttggggttt ttctgtggg cacatcatg aaaaaatgg tcgttgtgg accaaagtt acgcgtttg ttggcggca ttgatttgt gctatggac gctgggatg acgccaaac acagattga cagatcttg gaagcataa caccatcaa ttgacgttt caacgttga aaaagaaaa catcaagtg tgccaggag gcgacgcga tttacccaa tccctatcc cgatttaca aacaaacgc caatcccgg ttgtaaccg aaccggaag cacccgaac cttgccccc tccctcaac tagaacctg gcgacactc ttttaatac caactgtta aactcaaat gtaacactc ctgacatgg agcgcaaaa acgtaatag acacaatca aaaacacgg ttaaaacaa aaggaaaag atctcatct cattggttt acactcctc atgaagata gcgtcgaga aaacagagg aagagaaaa aaccacaaa cacaggacg agtttttgg aaactttga acggatcat cgaaattgt gggttggga aggattatg tcaatttta ttgttaagg attttaata atttggatt cgccgtttg atgatttta tcgatcggg tgaccacac ctcggatgc cgtggcatg atgtggggg tttgtgttc agggggctg ccgctagag acgtctcga gacatttta tccaacggt ggaatgcca ccaagttgg agaaagcca agtcgaata aatgctatc cttatttat tgccgaaaa gttacgacg attttaaaa ctttgccga ttgtgttcc cgaacgaag ttagcaacg tttcatgcc aggtcctcc gaagtgtta gtacatggt cttgtggtt ttttagaac cagatacag tcgagcaaa gcattcacg aggcctata ttgacacta ttaacaaag cccagcatt atatttaca tagaaaatc agtttttca tctcactgc cgtacacca atccaaaca caaggaacc aaatagccg aagcgcttt acaaacgaa ttgtccgag ctttcaaag ccaaagaag tgttccgtg tctttgttg ttatgccgc tgttgcctg gctttgagg gtgaagttg gggggccca caggcacct ccctgcatg ccataacac actggaatt attgctcaa tctcgcaag gtaaagatg cgattttga gtcggttac aagaggcag ggattgagg atccgagcg attttattt cgttctacg gtttgagaa atcatgcga ctttaaact gtgagccaa tcactgaat tgatttacg tccactcca agttaatga tagtcgatg ataaaactg tgatttgtg gctcggcta atattaacg atcgctctc taatcggta aaagagatt cggaaattg ccgttttga tcgaagatg aggccttcg atgatggcg tgatgaacg gaaagaagt tcccatgcg gaaaattcg cagggggtt tacgtaaat atttattca aggaacatt taggtttgt tggggaagg agcacgaaa tgatcgatt ttgacataa ccgatccaa tctcggatt atttctata aagaagttt ggtacaaaa cggccagtt tgaatacag agttttacg aaaaagtct tccactgta ttccaacag acagggtga aaaatttcg ccgatttga agaaaaatc aagaggaga agcccctgt atagcaacg agatttcgc gggctgaaa aaatgttgg agagtattc agggtcacc ttgtactct tgcctttac atttcctga gtcaggaaa gcctgactc ctgccgcta gttcagtag aagggatga tgccaacgt ccttgtgga cttaaaact gatattacg accgtccat aaacaggtg ctacaattt ttagccaac agcatttcc acaaagtga cttaaatgt gtgcgcaaa taacaaagc ttccaaaga tattattta ttgtgtcag tcgttgttt taatttgtt acaactttt acagtatta tacattttt agaaaccat gttcgcatt tactctgtt tatgtcagc taattacag ttatttatc taagttggc tcgtttaaa tcatttacc ttcactgtt tacaaatta ccgatcagg taggtaaag caatgtttt ctccaaata aataggata ttttata
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