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Gene information
General information
TC007750 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 522581..523456
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007750
    iBeetle screen iB_01258
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    remarks: later than schedule, entire repetition development delayed, hours 72
    Pupal morphology
    remarks: later than schedule, entire repetition development delayed, hours 72
    Adult morphology
    remarks: later than schedule, entire repetition development delayed, hours 72
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007750 RA
      MARANTIFD SAHKGDFEL VKSKLDEDP 
      SLLTKLDEN DRNLLHWVC VGGNFKLAT 
      HLIDLGAPI DARDDTDTT PLILASSAG 
      HCEIVSLLL EKGANVNQQ SSEGHSALQ 
      YAASKGWFS ICAKLVEHK ANVNISDKR 
      GATPLHRAA SKGNLEIVR LLIESPQHL 
      NINARDVYG FTPLHLACE EDRQDVAKL 
      LVQNGGDLD TKNRDGQTP LDLCTMHLR 
      KALEIIKSK IQ
    CDS sequence
    • >TC007750 RA
      atggcaaga gctaacaca atcttcgac tcggcccac 
      aaaggtgac ttcgagctt gtaaaaagc aaattggac 
      gaagacccc tctcttcta accaaacta gatgagaat 
      gacagaaat ttgctacac tgggtttgc gttgggggc 
      aattttaaa ctcgccaca cacttaatt gacctgggg 
      gcccccatt gacgcccgt gacgacacc gacacaacc 
      cccttaatt ctggcaagt tcggccggt cattgcgaa 
      atcgtgagt ctcttgctt gagaagggg gccaatgtg 
      aatcagcag tcaagcgag gggcactct gcccttcaa 
      tatgccgct tctaaaggc tggttttct atttgtgcc 
      aaattggtc gaacacaag gccaatgtt aacataagt 
      gataaacgg ggggccact ccgttacac agggctgcc 
      tccaagggg aacctagag attgtgaga ctgttgatt 
      gaaagtccg caacacttg aatataaac gcgcgagat 
      gtttatggg tttacgccg ctccatttg gcctgtgag 
      gaggataga caagatgtc gccaagttg ttggtgcaa 
      aatgggggc gacttggat acgaaaaat cgcgacggg 
      cagacgccc cttgatttg tgtacaatg catttgaga 
      aaagcgctc gaaattatc aaatcgaag atacaataa
    mRNA sequence
    • >TC007750 RA
      ttagaccta cttcatatt ttgatattc cttgttgat 
      ctctcgtta ttttgcgac aaaccgtgt taatggtat 
      ggcaagagc taacacaat cttcgactc ggcccacaa 
      aggtgactt cgagcttgt aaaaagcaa attggacga 
      agacccctc tcttctaac caaactaga tgagaatga 
      cagaaattt gctacactg ggtttgcgt tgggggcaa 
      ttttaaact cgccacaca cttaattga cctgggggc 
      ccccattga cgcccgtga cgacaccga cacaacccc 
      cttaattct ggcaagttc ggccggtca ttgcgaaat 
      cgtgagtct cttgcttga gaagggggc caatgtgaa 
      tcagcagtc aagcgaggg gcactctgc ccttcaata 
      tgccgcttc taaaggctg gttttctat ttgtgccaa 
      attggtcga acacaaggc caatgttaa cataagtga 
      taaacgggg ggccactcc gttacacag ggctgcctc 
      caaggggaa cctagagat tgtgagact gttgattga 
      aagtccgca acacttgaa tataaacgc gcgagatgt 
      ttatgggtt tacgccgct ccatttggc ctgtgagga 
      ggatagaca agatgtcgc caagttgtt ggtgcaaaa 
      tgggggcga cttggatac gaaaaatcg cgacgggca 
      gacgcccct tgatttgtg tacaatgca tttgagaaa 
      agcgctcga aattatcaa atcgaagat acaataatt 
      ttggtttta ataaagtag tgaaagcga a