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Gene information
General information
TC007749 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 526018..529267
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007749
    iBeetle screen iB_01257

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007749 RA
      MNEAGPATL MPKPDFTKC KIVKEGWLY KRGEHIRNW 
      RRRYFILLD DGSLLGYKN KPDETSMHD PLNNFTVKG 
      SQIMLADKP RLYTFTMRG LQWTTVIER MFHVDTERE 
      REEWVVAIK GVIDSLNEY CDDIEMNIE GNMDDLCEK 
      FSLQGTSIS STGKRKVTL ESFEFIKVL GKGTFGKVI 
      LCREKATGR LYAIKILKK EVIIQKDEV AHTQTENRV 
      LRKTNHPFL TSLKYSFQT NDRLCFVME YVNGGELFF 
      HLSRERVFS EDRTRFYGA EIISALAYL HSQNIIYRD 
      LKLENLLLD KDGHIKIAD FGLCKEDIT YGRTTKTFC 
      GTPEYLAPE VLEDIDYGR AVDWWGIGV VMYEMMVGR 
      LPFYNRDHD VLFQLILMD DVKFPRNLS SDARDLLGG 
      LLVKDPAKR LGGGPDDAK EIMAHPFFS CINWRDLEQ 
      KKIMPPFKP QVTSDTDTR YFDSEFTGE SVELTPPDN 
      SGPLGAIQE EPYFPQFSY QDMSSTLGS SAHISGSMN 
      NMATMQ
    CDS sequence
    • >TC007749 RA
      atgaacgaa gcaggtccg gccactttg atgcccaag 
      cccgacttc accaagtgc aaaattgtg aaggaaggg 
      tggctttat aaacgtggg gaacatatt aggaattgg 
      cgtaggagg tactttata cttttggat gatgggtct 
      ttattaggg tataagaat aagccagat gagacttca 
      atgcatgac cccttgaat aattttact gtgaagggg 
      tcgcagatc atgttggcc gataaacca aggctttat 
      acgtttaca atgaggggg ctgcagtgg accacggtc 
      atagaaagg atgtttcat gtggataca gaacgggaa 
      agagaagag tgggtggtg gccattaag ggcgtgata 
      gacagcctt aacgaatat tgtgacgat attgagatg 
      aatatagag gggaatatg gacgattta tgtgagaaa 
      ttctccctg caaggcact tcaatttcg tcaactggc 
      aaaagaaaa gttacttta gaaagtttc gaatttatt 
      aaagtgtta ggaaaagga acgtttggt aaagtgatt 
      ttgtgtcga gagaaagcc acaggtcga ctatatgcc 
      attaaaatt ctcaaaaaa gaagtgatt atacaaaag 
      gacgaagtc gctcacaca caaaccgaa aatcgggtc 
      ttacgtaaa acgaatcat ccattttta acctcgtta 
      aaatactcg ttccaaacc aacgatcgc ctctgtttt 
      gtcatggaa tatgtaaat ggtggcgaa ctcttcttt 
      catttatca cgcgaacgt gttttttcc gaagaccga 
      acacgtttt tacggtgct gaaataatc tcagctttg 
      gcatatctg cattcgcag aacattatt tacagggat 
      ttgaaatta gaaaatttg ttattggac aaagatgga 
      catataaaa atcgccgat tttggtcta tgtaaagaa 
      gatataacc tatggaaga acaactaaa acgttctgt 
      ggtacaccg gaatattta gcaccggaa gttttggaa 
      gatatcgat tatggacga gctgttgat tggtgggga 
      atcggtgtt gtgatgtac gaaatgatg gttgggcgc 
      ctaccattc tacaaccgg gaccacgac gtgttgttt 
      caattgatt ctaatggat gatgttaaa tttccgaga 
      aatttaagc tcagatgcg agagattta ttaggtgga 
      cttttggtc aaagatcca gctaaaaga ctaggaggt 
      ggtccagat gatgcaaaa gagataatg gcacatccg 
      ttttttagt tgtatcaat tggagggat cttgagcag 
      aaaaaaatt atgccgccg tttaaaccc caggttact 
      agcgatacg gatactaga tatttcgat tcggaattt 
      acgggtgaa agtgtcgaa ttgacaccg cctgataat 
      tccggtccg ctaggtgcc atacaagaa gagccttat 
      tttcctcaa tttagctat caagatatg tcttccacg 
      ttaggtagc tcagcacat ataagtggc agtatgaac 
      aacatggcc accatgcag tga
    mRNA sequence
    • >TC007749 RA
      aggatttga caaaaatat ctgcgtcaa aacacgtcc 
      ttgtgtatt ccaaattgc attttcccc aaatttggc 
      caaaaagcc caattcttg cgtaaaaaa tgcccaatg 
      tttgcaaaa ccgcgtcac ctgtgaacg aaccaaccg 
      actaaattt tttagtaaa aatttgtat gcccctcgt 
      gtgtgaaaa atgaacgaa gcaggtccg gccactttg 
      atgcccaag cccgacttc accaagtgc aaaattgtg 
      aaggaaggg tggctttat aaacgtggg gaacatatt 
      aggaattgg cgtaggagg tactttata cttttggat 
      gatgggtct ttattaggg tataagaat aagccagat 
      gagacttca atgcatgac cccttgaat aattttact 
      gtgaagggg tcgcagatc atgttggcc gataaacca 
      aggctttat acgtttaca atgaggggg ctgcagtgg 
      accacggtc atagaaagg atgtttcat gtggataca 
      gaacgggaa agagaagag tgggtggtg gccattaag 
      ggcgtgata gacagcctt aacgaatat tgtgacgat 
      attgagatg aatatagag gggaatatg gacgattta 
      tgtgagaaa ttctccctg caaggcact tcaatttcg 
      tcaactggc aaaagaaaa gttacttta gaaagtttc 
      gaatttatt aaagtgtta ggaaaagga acgtttggt 
      aaagtgatt ttgtgtcga gagaaagcc acaggtcga 
      ctatatgcc attaaaatt ctcaaaaaa gaagtgatt 
      atacaaaag gacgaagtc gctcacaca caaaccgaa 
      aatcgggtc ttacgtaaa acgaatcat ccattttta 
      acctcgtta aaatactcg ttccaaacc aacgatcgc 
      ctctgtttt gtcatggaa tatgtaaat ggtggcgaa 
      ctcttcttt catttatca cgcgaacgt gttttttcc 
      gaagaccga acacgtttt tacggtgct gaaataatc 
      tcagctttg gcatatctg cattcgcag aacattatt 
      tacagggat ttgaaatta gaaaatttg ttattggac 
      aaagatgga catataaaa atcgccgat tttggtcta 
      tgtaaagaa gatataacc tatggaaga acaactaaa 
      acgttctgt ggtacaccg gaatattta gcaccggaa 
      gttttggaa gatatcgat tatggacga gctgttgat 
      tggtgggga atcggtgtt gtgatgtac gaaatgatg 
      gttgggcgc ctaccattc tacaaccgg gaccacgac 
      gtgttgttt caattgatt ctaatggat gatgttaaa 
      tttccgaga aatttaagc tcagatgcg agagattta 
      ttaggtgga cttttggtc aaagatcca gctaaaaga 
      ctaggaggt ggtccagat gatgcaaaa gagataatg 
      gcacatccg ttttttagt tgtatcaat tggagggat 
      cttgagcag aaaaaaatt atgccgccg tttaaaccc 
      caggttact agcgatacg gatactaga tatttcgat 
      tcggaattt acgggtgaa agtgtcgaa ttgacaccg 
      cctgataat tccggtccg ctaggtgcc atacaagaa 
      gagccttat tttcctcaa tttagctat caagatatg 
      tcttccacg ttaggtagc tcagcacat ataagtggc 
      agtatgaac aacatggcc accatgcag tgactcagt 
      cggatcaaa actatagac actctgtat atatcatag 
      ttaattcgc tgttttaaa agccactgt acttatcgt 
      tgcgaagtg taaataatt tacgattct gttgattgt 
      tagtgactt ttttccttt ttttttact attattcta 
      gtgtatata ttttacgta ctgtaatgt ttaaattag 
      tgtgatttt ctataattt cgtgcattt aactcgtat 
      acgaatgtt aaacaaatt catttgaga tttattcta 
      atattattg tgggcggaa aatattttt gtgtggcta 
      atatttagt ttgtatata caattgttg ctatctttg 
      ggctactct gtaaatatg gtggttttt aatcatgaa 
      atttccgcg gagtattta tttatattt cccaaaagt 
      gtaggaaaa aaataaact atttgtttt gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY