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Gene information
General information
TC007745 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 510498..516232
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007745
    iBeetle screen iB_01256
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
    egg chamber number decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007745 RA
      MALPANYKQ VAMQMPSPQ TNHLLSAGV PMTFNILKE 
      RLRASGGSG SGSNKETSP FVTTPNGHP GFLPQKVGK 
      SSSHYANNF YYDYNPFYY YYNNSNYNQ YKSSYNYYY 
      VQNDSRTPK PPKAPEKPL MPYMRYSRK VWDTVKAQN 
      PDLKLWEIG RIIGQMWRD LPEEEKNEF VEEYEAEKL 
      EYEKTLKTY HNSPAYQAF IAAKNRGKS GNDSDSHDR 
      SSSSSKQQA VDRRIEIQA AEDEDDQDD GYSVKHVSY 
      ARFLRNHRL INEIFSDTV VPDVRSVVT TGRMQVLKR 
      QVQSLTMHQ KKLEAELQQ IEERFEAKK RKFIESSEQ 
      FQEELKKHC KPAVDEETF QKMVERQYD LLKKERMKS 
      LEEQKPKPE ENGAATPTE IANSGAQPE QPPPAQEGQ 
      PQSEPMEQD DKPAPSPPP PSNENKETP EHKPPPEQP 
      PLEHPPPHM PPPMHHHVP PAPNMMPPY QQYPQNSPQ 
      TVPLPPRPP HPAYAYPQQ QGYYPPQYP PHTYYHQPY 
      PPQYHRPHL YPPHGPVPD GQAMPPNPE GHPAPIYGG 
      PAPAEAERV QPSEEEQTK HEGSAEGEK DKKEGE
    • >TC007745 RB
      MALPANYKQ VAMQMPSPQ TNHLLSAGV PMTFNILKE 
      RLRASGGSG SGSNLFLQK ETSPFVTTP NGHPGFLPQ 
      KVGKSSSHY ANNFYYDYN PFYYYYNNS NYNQYKSSY 
      NYYYVQNDS RTPKPPKAP EKPLMPYMR YSRKVWDTV 
      KAQNPDLKL WEIGRIIGQ MWRDLPEEE KNEFVEEYE 
      AEKLEYEKT LKTYHNSPA YQAFIAAKN RGKSGNDSD 
      SHDRSSSSS KQQAVDRRI EIQAAEDED DQDDGYSVK 
      HVSYARFLR NHRLINEIF SDTVVPDVR SVVTTGRMQ 
      VLKRQVQSL TMHQKKLEA ELQQIEERF EAKKRKFIE 
      SSEQFQEEL KKHCKPAVD EETFQKMVE RQYDLLKKE 
      RMKSLEEQK PKPEENGAA TPTEIANSG AQPEQPPPA 
      QEGQPQSEP MEQDDKPAP SPPPPSNEN KETPEHKPP 
      PEQPPLEHP PPHMPPPMH HHVPPAPNM MPPYQQYPQ 
      NSPQTVPLP PRPPHPAYA YPQQQGYYP PQYPPHTYY 
      HQPYPPQYH RPHLYPPHG PVPDGQAMP PNPEGHPAP 
      IYGGPAPAE AERVQPSEE EQTKHEGSA EGEKDKKEG 
      E
    • >TC007745 RC
      MALPANYKQ VAMQMPSPQ TNQRLRASG GSGSGSNLF 
      LQKETSPFV TTPNGHPGF LPQKVGKSS SHYANNFYY 
      DYNPFYYYY NNSNYNQYK SSYNYYYVQ NDSRTPKPP 
      KAPEKPLMP YMRYSRKVW DTVKAQNPD LKLWEIGRI 
      IGQMWRDLP EEEKNEFVE EYEAEKLEY EKTLKTYHN 
      SPAYQAFIA AKNRGKSGN DSDSHDRSS SSSKQQAVD 
      RRIEIQAAE DEDDQDDGY SVKHVSYAR FLRNHRLIN 
      EIFSDTVVP DVRSVVTTG RMQVLKRQV QSLTMHQKK 
      LEAELQQIE ERFEAKKRK FIESSEQFQ EELKKHCKP 
      AVDEETFQK MVERQYDLL KKERMKSLE EQKPKPEEN 
      GAATPTEIA NSGAQPEQP PPAQEGQPQ SEPMEQDDK 
      PAPSPPPPS NENKETPEH KPPPEQPPL EHPPPHMPP 
      PMHHHVPPA PNMMPPYQQ YPQNSPQTV PLPPRPPHP 
      AYAYPQQQG YYPPQYPPH TYYHQPYPP QYHRPHLYP 
      PHGPVPDGQ AMPPNPEGH PAPIYGGPA PAEAERVQP 
      SEEEQTKHE GSAEGEKDK KEGE
    CDS sequence
    • >TC007745 RA
      atggctctt ccagccaat tacaagcaa gtagcgatg 
      caaatgcct agccctcaa acaaaccat ttgctgtcg 
      gcaggcgtg cccatgact ttcaacata ttgaaagag 
      cgtttgaga gcgtctgga gggtccgga tctggatca 
      aataaagaa acaagtccg tttgtaaca acgccgaat 
      ggccatcct gggttttta ccacaaaaa gtgggaaag 
      agttcgtcg cactacgct aacaatttc tattacgat 
      tacaatcct ttttattat tattacaat aatagcaat 
      tacaatcag tacaaaagt agttataat tactactac 
      gtccagaat gattcgcgt actcctaag ccccccaaa 
      gcgcccgaa aagcctctc atgccctac atgagatac 
      agtcgaaag gtttgggat acggttaaa gcacaaaat 
      cccgatttg aaattatgg gaaattgga cgaattatc 
      gggcaaatg tggagggat ctccccgag gaagaaaaa 
      aacgaattt gttgaggaa tacgaagct gaaaagttg 
      gaatatgaa aaaacgctg aaaacttat cacaattca 
      ccggcttat caggcattt atcgctgct aaaaatcga 
      ggtaaaagt ggcaatgat agtgattcg catgatcgc 
      agcagttcg tcatcgaaa caacaagcc gtcgatcga 
      aggattgaa attcaagca gcggaggac gaagatgat 
      caggacgat ggctattcg gtgaaacac gtttcctac 
      gcccgcttt ctgcgcaat catcgtctc attaacgaa 
      attttttcc gatactgtt gtaccggat gtccgttca 
      gttgttaca acaggtcgt atgcaagtt ctcaaacgt 
      caagtccaa tcattgaca atgcatcag aaaaaactc 
      gaagccgaa ttgcaacaa atcgaagaa cgatttgaa 
      gcaaaaaaa cgcaaattt atcgaaagt agtgaacag 
      tttcaagag gaattgaag aaacattgc aagccggct 
      gttgatgaa gaaaccttt cagaaaatg gtcgaacga 
      caatacgat ttattgaag aaggagaga atgaagagt 
      ttggaagaa cagaaaccg aaaccggaa gaaaatggg 
      gctgctacg cccactgaa attgctaat tcgggggca 
      caaccggaa caaccgccc ccagctcaa gagggacag 
      ccccaatct gagccaatg gaacaagac gataaacca 
      gccccttca ccgccgccc ccatcgaac gaaaacaaa 
      gaaaccccc gagcataaa ccgcccccg gaacagcct 
      ccactcgag catccaccc ccgcacatg ccacctccc 
      atgcaccac cacgtgcct ccagcgccc aacatgatg 
      cccccttat cagcagtac ccccaaaac agtccacag 
      actgttcct ctgccccca aggccccca catccggcc 
      tacgcctac ccccaacaa caggggtat tacccccct 
      cagtacccc ccacatacg tactaccat caaccgtac 
      ccaccacag taccataga ccccatttg tacccaccc 
      catgggccg gtaccagat gggcaagcc atgcccccc 
      aatccggag gggcaccca gcccccatt tatggaggc 
      cctgcacca gcagaggct gaaagggtg caaccgagt 
      gaagaggag cagactaaa cacgagggc agcgctgaa 
      ggtgaaaag gacaagaaa gaaggggaa tag
    • >TC007745 RB
      atggctctt ccagccaat tacaagcaa gtagcgatg 
      caaatgcct agccctcaa acaaaccat ttgctgtcg 
      gcaggcgtg cccatgact ttcaacata ttgaaagag 
      cgtttgaga gcgtctgga gggtccgga tctggatca 
      aatttattt ttacagaaa gaaacaagt ccgtttgta 
      acaacgccg aatggccat cctgggttt ttaccacaa 
      aaagtggga aagagttcg tcgcactac gctaacaat 
      ttctattac gattacaat cctttttat tattattac 
      aataatagc aattacaat cagtacaaa agtagttat 
      aattactac tacgtccag aatgattcg cgtactcct 
      aagcccccc aaagcgccc gaaaagcct ctcatgccc 
      tacatgaga tacagtcga aaggtttgg gatacggtt 
      aaagcacaa aatcccgat ttgaaatta tgggaaatt 
      ggacgaatt atcgggcaa atgtggagg gatctcccc 
      gaggaagaa aaaaacgaa tttgttgag gaatacgaa 
      gctgaaaag ttggaatat gaaaaaacg ctgaaaact 
      tatcacaat tcaccggct tatcaggca tttatcgct 
      gctaaaaat cgaggtaaa agtggcaat gatagtgat 
      tcgcatgat cgcagcagt tcgtcatcg aaacaacaa 
      gccgtcgat cgaaggatt gaaattcaa gcagcggag 
      gacgaagat gatcaggac gatggctat tcggtgaaa 
      cacgtttcc tacgcccgc tttctgcgc aatcatcgt 
      ctcattaac gaaattttt tccgatact gttgtaccg 
      gatgtccgt tcagttgtt acaacaggt cgtatgcaa 
      gttctcaaa cgtcaagtc caatcattg acaatgcat 
      cagaaaaaa ctcgaagcc gaattgcaa caaatcgaa 
      gaacgattt gaagcaaaa aaacgcaaa tttatcgaa 
      agtagtgaa cagtttcaa gaggaattg aagaaacat 
      tgcaagccg gctgttgat gaagaaacc tttcagaaa 
      atggtcgaa cgacaatac gatttattg aagaaggag 
      agaatgaag agtttggaa gaacagaaa ccgaaaccg 
      gaagaaaat ggggctgct acgcccact gaaattgct 
      aattcgggg gcacaaccg gaacaaccg cccccagct 
      caagaggga cagccccaa tctgagcca atggaacaa 
      gacgataaa ccagcccct tcaccgccg cccccatcg 
      aacgaaaac aaagaaacc cccgagcat aaaccgccc 
      ccggaacag cctccactc gagcatcca cccccgcac 
      atgccacct cccatgcac caccacgtg cctccagcg 
      cccaacatg atgccccct tatcagcag tacccccaa 
      aacagtcca cagactgtt cctctgccc ccaaggccc 
      ccacatccg gcctacgcc tacccccaa caacagggg 
      tattacccc cctcagtac cccccacat acgtactac 
      catcaaccg tacccacca cagtaccat agaccccat 
      ttgtaccca ccccatggg ccggtacca gatgggcaa 
      gccatgccc cccaatccg gaggggcac ccagccccc 
      atttatgga ggccctgca ccagcagag gctgaaagg 
      gtgcaaccg agtgaagag gagcagact aaacacgag 
      ggcagcgct gaaggtgaa aaggacaag aaagaaggg 
      gaatag
    • >TC007745 RC
      atggctctt ccagccaat tacaagcaa gtagcgatg 
      caaatgcct agccctcaa acaaaccag cgtttgaga 
      gcgtctgga gggtccgga tctggatca aatttattt 
      ttacagaaa gaaacaagt ccgtttgta acaacgccg 
      aatggccat cctgggttt ttaccacaa aaagtggga 
      aagagttcg tcgcactac gctaacaat ttctattac 
      gattacaat cctttttat tattattac aataatagc 
      aattacaat cagtacaaa agtagttat aattactac 
      tacgtccag aatgattcg cgtactcct aagcccccc 
      aaagcgccc gaaaagcct ctcatgccc tacatgaga 
      tacagtcga aaggtttgg gatacggtt aaagcacaa 
      aatcccgat ttgaaatta tgggaaatt ggacgaatt 
      atcgggcaa atgtggagg gatctcccc gaggaagaa 
      aaaaacgaa tttgttgag gaatacgaa gctgaaaag 
      ttggaatat gaaaaaacg ctgaaaact tatcacaat 
      tcaccggct tatcaggca tttatcgct gctaaaaat 
      cgaggtaaa agtggcaat gatagtgat tcgcatgat 
      cgcagcagt tcgtcatcg aaacaacaa gccgtcgat 
      cgaaggatt gaaattcaa gcagcggag gacgaagat 
      gatcaggac gatggctat tcggtgaaa cacgtttcc 
      tacgcccgc tttctgcgc aatcatcgt ctcattaac 
      gaaattttt tccgatact gttgtaccg gatgtccgt 
      tcagttgtt acaacaggt cgtatgcaa gttctcaaa 
      cgtcaagtc caatcattg acaatgcat cagaaaaaa 
      ctcgaagcc gaattgcaa caaatcgaa gaacgattt 
      gaagcaaaa aaacgcaaa tttatcgaa agtagtgaa 
      cagtttcaa gaggaattg aagaaacat tgcaagccg 
      gctgttgat gaagaaacc tttcagaaa atggtcgaa 
      cgacaatac gatttattg aagaaggag agaatgaag 
      agtttggaa gaacagaaa ccgaaaccg gaagaaaat 
      ggggctgct acgcccact gaaattgct aattcgggg 
      gcacaaccg gaacaaccg cccccagct caagaggga 
      cagccccaa tctgagcca atggaacaa gacgataaa 
      ccagcccct tcaccgccg cccccatcg aacgaaaac 
      aaagaaacc cccgagcat aaaccgccc ccggaacag 
      cctccactc gagcatcca cccccgcac atgccacct 
      cccatgcac caccacgtg cctccagcg cccaacatg 
      atgccccct tatcagcag tacccccaa aacagtcca 
      cagactgtt cctctgccc ccaaggccc ccacatccg 
      gcctacgcc tacccccaa caacagggg tattacccc 
      cctcagtac cccccacat acgtactac catcaaccg 
      tacccacca cagtaccat agaccccat ttgtaccca 
      ccccatggg ccggtacca gatgggcaa gccatgccc 
      cccaatccg gaggggcac ccagccccc atttatgga 
      ggccctgca ccagcagag gctgaaagg gtgcaaccg 
      agtgaagag gagcagact aaacacgag ggcagcgct 
      gaaggtgaa aaggacaag aaagaaggg gaatag
    mRNA sequence
    • >TC007745 RA
      aacatggcg tcatttgtg taaaaagtg ttgtgtatt 
      ttaaaagaa tctcaattt tagatctaa tttaattag 
      tgtgtgtgt gaaaaaatg gctcttcca gccaattac 
      aagcaagta gcgatgcaa atgcctagc cctcaaaca 
      aaccatttg ctgtcggca ggcgtgccc atgactttc 
      aacatattg aaagagcgt ttgagagcg tctggaggg 
      tccggatct ggatcaaat aaagaaaca agtccgttt 
      gtaacaacg ccgaatggc catcctggg tttttacca 
      caaaaagtg ggaaagagt tcgtcgcac tacgctaac 
      aatttctat tacgattac aatcctttt tattattat 
      tacaataat agcaattac aatcagtac aaaagtagt 
      tataattac tactacgtc cagaatgat tcgcgtact 
      cctaagccc cccaaagcg cccgaaaag cctctcatg 
      ccctacatg agatacagt cgaaaggtt tgggatacg 
      gttaaagca caaaatccc gatttgaaa ttatgggaa 
      attggacga attatcggg caaatgtgg agggatctc 
      cccgaggaa gaaaaaaac gaatttgtt gaggaatac 
      gaagctgaa aagttggaa tatgaaaaa acgctgaaa 
      acttatcac aattcaccg gcttatcag gcatttatc 
      gctgctaaa aatcgaggt aaaagtggc aatgatagt 
      gattcgcat gatcgcagc agttcgtca tcgaaacaa 
      caagccgtc gatcgaagg attgaaatt caagcagcg 
      gaggacgaa gatgatcag gacgatggc tattcggtg 
      aaacacgtt tcctacgcc cgctttctg cgcaatcat 
      cgtctcatt aacgaaatt ttttccgat actgttgta 
      ccggatgtc cgttcagtt gttacaaca ggtcgtatg 
      caagttctc aaacgtcaa gtccaatca ttgacaatg 
      catcagaaa aaactcgaa gccgaattg caacaaatc 
      gaagaacga tttgaagca aaaaaacgc aaatttatc 
      gaaagtagt gaacagttt caagaggaa ttgaagaaa 
      cattgcaag ccggctgtt gatgaagaa acctttcag 
      aaaatggtc gaacgacaa tacgattta ttgaagaag 
      gagagaatg aagagtttg gaagaacag aaaccgaaa 
      ccggaagaa aatggggct gctacgccc actgaaatt 
      gctaattcg ggggcacaa ccggaacaa ccgccccca 
      gctcaagag ggacagccc caatctgag ccaatggaa 
      caagacgat aaaccagcc ccttcaccg ccgccccca 
      tcgaacgaa aacaaagaa acccccgag cataaaccg 
      cccccggaa cagcctcca ctcgagcat ccacccccg 
      cacatgcca cctcccatg caccaccac gtgcctcca 
      gcgcccaac atgatgccc ccttatcag cagtacccc 
      caaaacagt ccacagact gttcctctg cccccaagg 
      cccccacat ccggcctac gcctacccc caacaacag 
      gggtattac ccccctcag tacccccca catacgtac 
      taccatcaa ccgtaccca ccacagtac catagaccc 
      catttgtac ccaccccat gggccggta ccagatggg 
      caagccatg ccccccaat ccggagggg cacccagcc 
      cccatttat ggaggccct gcaccagca gaggctgaa 
      agggtgcaa ccgagtgaa gaggagcag actaaacac 
      gagggcagc gctgaaggt gaaaaggac aagaaagaa 
      ggggaatag gtgttgggg attttttgg ttacttttg 
      caataaatt gagtggatg cta
    • >TC007745 RB
      aacatggcg tcatttgtg taaaaagtg ttgtgtatt 
      ttaaaagaa tctcaattt tagatctaa tttaattag 
      tgtgtgtgt gaaaaaatg gctcttcca gccaattac 
      aagcaagta gcgatgcaa atgcctagc cctcaaaca 
      aaccatttg ctgtcggca ggcgtgccc atgactttc 
      aacatattg aaagagcgt ttgagagcg tctggaggg 
      tccggatct ggatcaaat ttattttta cagaaagaa 
      acaagtccg tttgtaaca acgccgaat ggccatcct 
      gggttttta ccacaaaaa gtgggaaag agttcgtcg 
      cactacgct aacaatttc tattacgat tacaatcct 
      ttttattat tattacaat aatagcaat tacaatcag 
      tacaaaagt agttataat tactactac gtccagaat 
      gattcgcgt actcctaag ccccccaaa gcgcccgaa 
      aagcctctc atgccctac atgagatac agtcgaaag 
      gtttgggat acggttaaa gcacaaaat cccgatttg 
      aaattatgg gaaattgga cgaattatc gggcaaatg 
      tggagggat ctccccgag gaagaaaaa aacgaattt 
      gttgaggaa tacgaagct gaaaagttg gaatatgaa 
      aaaacgctg aaaacttat cacaattca ccggcttat 
      caggcattt atcgctgct aaaaatcga ggtaaaagt 
      ggcaatgat agtgattcg catgatcgc agcagttcg 
      tcatcgaaa caacaagcc gtcgatcga aggattgaa 
      attcaagca gcggaggac gaagatgat caggacgat 
      ggctattcg gtgaaacac gtttcctac gcccgcttt 
      ctgcgcaat catcgtctc attaacgaa attttttcc 
      gatactgtt gtaccggat gtccgttca gttgttaca 
      acaggtcgt atgcaagtt ctcaaacgt caagtccaa 
      tcattgaca atgcatcag aaaaaactc gaagccgaa 
      ttgcaacaa atcgaagaa cgatttgaa gcaaaaaaa 
      cgcaaattt atcgaaagt agtgaacag tttcaagag 
      gaattgaag aaacattgc aagccggct gttgatgaa 
      gaaaccttt cagaaaatg gtcgaacga caatacgat 
      ttattgaag aaggagaga atgaagagt ttggaagaa 
      cagaaaccg aaaccggaa gaaaatggg gctgctacg 
      cccactgaa attgctaat tcgggggca caaccggaa 
      caaccgccc ccagctcaa gagggacag ccccaatct 
      gagccaatg gaacaagac gataaacca gccccttca 
      ccgccgccc ccatcgaac gaaaacaaa gaaaccccc 
      gagcataaa ccgcccccg gaacagcct ccactcgag 
      catccaccc ccgcacatg ccacctccc atgcaccac 
      cacgtgcct ccagcgccc aacatgatg cccccttat 
      cagcagtac ccccaaaac agtccacag actgttcct 
      ctgccccca aggccccca catccggcc tacgcctac 
      ccccaacaa caggggtat tacccccct cagtacccc 
      ccacatacg tactaccat caaccgtac ccaccacag 
      taccataga ccccatttg tacccaccc catgggccg 
      gtaccagat gggcaagcc atgcccccc aatccggag 
      gggcaccca gcccccatt tatggaggc cctgcacca 
      gcagaggct gaaagggtg caaccgagt gaagaggag 
      cagactaaa cacgagggc agcgctgaa ggtgaaaag 
      gacaagaaa gaaggggaa taggtgttg gggattttt 
      tggttactt ttgcaataa attgagtgg atgcta
    • >TC007745 RC
      aacatggcg tcatttgtg taaaaagtg ttgtgtatt 
      ttaaaagaa tctcaattt tagatctaa tttaattag 
      tgtgtgtgt gaaaaaatg gctcttcca gccaattac 
      aagcaagta gcgatgcaa atgcctagc cctcaaaca 
      aaccagcgt ttgagagcg tctggaggg tccggatct 
      ggatcaaat ttattttta cagaaagaa acaagtccg 
      tttgtaaca acgccgaat ggccatcct gggttttta 
      ccacaaaaa gtgggaaag agttcgtcg cactacgct 
      aacaatttc tattacgat tacaatcct ttttattat 
      tattacaat aatagcaat tacaatcag tacaaaagt 
      agttataat tactactac gtccagaat gattcgcgt 
      actcctaag ccccccaaa gcgcccgaa aagcctctc 
      atgccctac atgagatac agtcgaaag gtttgggat 
      acggttaaa gcacaaaat cccgatttg aaattatgg 
      gaaattgga cgaattatc gggcaaatg tggagggat 
      ctccccgag gaagaaaaa aacgaattt gttgaggaa 
      tacgaagct gaaaagttg gaatatgaa aaaacgctg 
      aaaacttat cacaattca ccggcttat caggcattt 
      atcgctgct aaaaatcga ggtaaaagt ggcaatgat 
      agtgattcg catgatcgc agcagttcg tcatcgaaa 
      caacaagcc gtcgatcga aggattgaa attcaagca 
      gcggaggac gaagatgat caggacgat ggctattcg 
      gtgaaacac gtttcctac gcccgcttt ctgcgcaat 
      catcgtctc attaacgaa attttttcc gatactgtt 
      gtaccggat gtccgttca gttgttaca acaggtcgt 
      atgcaagtt ctcaaacgt caagtccaa tcattgaca 
      atgcatcag aaaaaactc gaagccgaa ttgcaacaa 
      atcgaagaa cgatttgaa gcaaaaaaa cgcaaattt 
      atcgaaagt agtgaacag tttcaagag gaattgaag 
      aaacattgc aagccggct gttgatgaa gaaaccttt 
      cagaaaatg gtcgaacga caatacgat ttattgaag 
      aaggagaga atgaagagt ttggaagaa cagaaaccg 
      aaaccggaa gaaaatggg gctgctacg cccactgaa 
      attgctaat tcgggggca caaccggaa caaccgccc 
      ccagctcaa gagggacag ccccaatct gagccaatg 
      gaacaagac gataaacca gccccttca ccgccgccc 
      ccatcgaac gaaaacaaa gaaaccccc gagcataaa 
      ccgcccccg gaacagcct ccactcgag catccaccc 
      ccgcacatg ccacctccc atgcaccac cacgtgcct 
      ccagcgccc aacatgatg cccccttat cagcagtac 
      ccccaaaac agtccacag actgttcct ctgccccca 
      aggccccca catccggcc tacgcctac ccccaacaa 
      caggggtat tacccccct cagtacccc ccacatacg 
      tactaccat caaccgtac ccaccacag taccataga 
      ccccatttg tacccaccc catgggccg gtaccagat 
      gggcaagcc atgcccccc aatccggag gggcaccca 
      gcccccatt tatggaggc cctgcacca gcagaggct 
      gaaagggtg caaccgagt gaagaggag cagactaaa 
      cacgagggc agcgctgaa ggtgaaaag gacaagaaa 
      gaaggggaa taggtgttg gggattttt tggttactt 
      ttgcaataa attgagtgg atgcta