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Gene information
General information
TC007733 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 291649..292936
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007733
    iBeetle screen iB_01254

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007733 RA
      MIVLLILIL EVVTIVQGG CPIRCTCTQ NSITCVQSS 
      LEYVPYFES LDNNPVIID LSGNPITLI DIDDFSFDK 
      SDQVKEIYL NSTSLSIID DHAFDELEN LQELYLSDN 
      LLHYFPEEI IRSNENMVL LDLSSNYFA EMPKIQSKS 
      LEVLAIANS RIERIGDDA LDALPNLRI LLLQQNNLK 
      SVNPVIFSG MNNLFFVGL AHNPWLCDC KTVELFEFL 
      ISKRFVDVT EPVRCQNEK KIFVELGGK CADFNVTKP 
      SNEIIVTTE FDNDFYYAL MLFVAIITS LVLGATCGT 
      CITYKFLTR SVKMSESKN ALLYDLRV
    CDS sequence
    • >TC007733 RA
      atgattgtg cttttaatt ctaattttg gaagtagtg 
      accatagtc caaggcggg tgccccatc cgttgtact 
      tgcacccaa aactcaata acttgtgtc cagtcaagt 
      ctagagtac gtgccttat ttcgaaagt ctggataac 
      aaccccgtc ataatcgac ctttcgggg aaccccata 
      acattaatc gacatcgac gatttcagc tttgataaa 
      agcgaccaa gtcaaagag atttatcta aacagtacc 
      tccttaagc attattgac gatcacgcc tttgacgag 
      cttgaaaac ttgcaagaa ctctacctc agcgataat 
      ttattgcac tatttcccc gaagaaatc attcggagt 
      aacgagaac atggtgcta ttggacttg tcgagtaat 
      tattttgcc gaaatgccc aaaattcag tccaagagt 
      ttggaggtt ttggcgatt gctaattcg cgaattgaa 
      cggattggt gatgacgct ttggacgcg ttaccaaat 
      ttgcgcatc ttgcttttg caacagaat aacttgaag 
      agtgttaat ccggtgatt ttctcgggg atgaataat 
      ttgtttttt gtggggctg gcccacaat ccgtggctg 
      tgtgattgc aagacggtt gaattattc gagtttctg 
      ataagtaaa cggtttgtt gatgttacg gaaccggtg 
      cggtgccaa aacgagaag aaaattttc gtggaattg 
      ggcggaaaa tgcgccgat ttcaacgtt acaaagccg 
      agtaacgaa attatagtg acgaccgaa tttgacaat 
      gatttttac tacgctttg atgcttttt gttgcgatt 
      attacgagt ctggttttg ggggcgact tgcggcact 
      tgtatcacg tacaaattc ctgactagg agtgtgaag 
      atgagcgag agtaaaaat gcgctgctt tacgacttg 
      agggtttaa
    mRNA sequence
    • >TC007733 RA
      gattagaaa catcgcagg tatctaaaa gtctgtaaa 
      caccatcca agtttcaat aacttgaat cgataagat 
      aaatcgtac gtaattcaa ggaacgagt taggttccc 
      aacgcaccg aaaaagcca caaaaattg aagtgataa 
      aatgattgt gcttttaat tctaatttt ggaagtagt 
      gaccatagt ccaaggcgg gtgccccat ccgttgtac 
      ttgcaccca aaactcaat aacttgtgt ccagtcaag 
      tctagagta cgtgcctta tttcgaaag tctggataa 
      caaccccgt cataatcga cctttcggg gaaccccat 
      aacattaat cgacatcga cgatttcag ctttgataa 
      aagcgacca agtcaaaga gatttatct aaacagtac 
      ctccttaag cattattga cgatcacgc ctttgacga 
      gcttgaaaa cttgcaaga actctacct cagcgataa 
      tttattgca ctatttccc cgaagaaat cattcggag 
      taacgagaa catggtgct attggactt gtcgagtaa 
      ttattttgc cgaaatgcc caaaattca gtccaagag 
      tttggaggt tttggcgat tgctaattc gcgaattga 
      acggattgg tgatgacgc tttggacgc gttaccaaa 
      tttgcgcat cttgctttt gcaacagaa taacttgaa 
      gagtgttaa tccggtgat tttctcggg gatgaataa 
      tttgttttt tgtggggct ggcccacaa tccgtggct 
      gtgtgattg caagacggt tgaattatt cgagtttct 
      gataagtaa acggtttgt tgatgttac ggaaccggt 
      gcggtgcca aaacgagaa gaaaatttt cgtggaatt 
      gggcggaaa atgcgccga tttcaacgt tacaaagcc 
      gagtaacga aattatagt gacgaccga atttgacaa 
      tgattttta ctacgcttt gatgctttt tgttgcgat 
      tattacgag tctggtttt gggggcgac ttgcggcac 
      ttgtatcac gtacaaatt cctgactag gagtgtgaa 
      gatgagcga gagtaaaaa tgcgctgct ttacgactt 
      gagggttta agtcacttt tgtataaat atttttttt 
      atttttcta actgtttta agttatgta aataaatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY