Status
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Gene information
General information
TC007718 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 125109..131134
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007718
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01253

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007718 RA
      MAESNIYYN EGNYMNQQD ATTKDVIDS VQNLNEQSE 
      MICDDSVTE TVEESKDEV KEVAEGTEE HVESPTTGE 
      TSYTFKCVF CEQVLSGSD EPKLLECLH NACGNCIGN 
      KLYEELEKT TVECPQCNI VCEPSKIIE NQFLLELAS 
      NEDNSTKLA ELKCSSCSD DAIATSWCV DCSEYICDS 
      CVQAHQRLK ITKDHTIKP KEEGLTDSQ SSTNNSAPN 
      LFCQIHPQE KLSLFCETC DKLTCRDCQ LIEHREHKY 
      KFTNEIASE TRKFIADML KDVGYKRAL LCSAMKVID 
      DRQNLIVDK KQALVKEIT QLVVKLTNT INVRGKQLV 
      SKLTEVCDS KQKTLQEKK QALEQLSRM TDHCIEFTQ 
      HALDNGSDM ALLYSKKQV TDHLRRIKC KRADIPNPE 
      IPVRIHLAL DKFPDLVKV MSTIGQIVV DGRIYPSQS 
      VSPSSQLPP HGSPSPGHS RPPQQQSPI NQLLQQSPP 
      YQQQPSGFQ QSPRLPPAY GQFNNGPQY PQNQNISPQ 
      SNMGARVMG PPMAQQISL AQQLSANRM QMPRQMARP 
      LLPRMPPNS GGLNQQQVS SSTHPQNML SDRSNLRCL 
      LQPASNSPV YIQTGPAQY QAVQPHMAQ QYQTRFPNQ 
      SQQSFRQQP PMNPMMMNQ NRPQRQFQM QGGSARWHI 
      PQQQIANNN NMQGGNRNM ILPTINDNF KITLKQHNQ 
      SENRNLPAT VTSTIPKTP SPNHIQGRS DTERSLDKF 
      CEDSVKDLM ATIAKLDSN GVQVVAEGR QKTGGSPHV 
      DSSTGDAGP SGKIKSDPM ETGKDDPNE DWCAVCMDG 
      GELVCCDKC PKVFHQYCH IPNLSVEEN DTWQCLLCT 
      NFADLSDDI LNEKKEGEL CYKDKKIAE RIVLELYCQ 
      YDPSLPFRE VIGPENEEY HAIIKTPIA LDSIRQKLN 
      WNSPEHYTT MEQLIKDVR LMFKNAYTY NPEDSQVYA 
      DAKVLEKFF DEQLEKFLP EYAYEHFDD DDDIQPPNK 
      KYRRIISD
    CDS sequence
    • >TC007718 RA
      atggctgaa tcaaatatt tattacaac gagggcaat 
      tatatgaat cagcaagac gctaccacc aaggatgtg 
      attgattca gttcagaat ctgaatgag cagagtgaa 
      atgatttgt gatgattct gtaacagaa acagtggaa 
      gaatcgaag gatgaagtt aaagaagtt gctgaaggc 
      actgaagaa catgtagag agtccaaca actggggaa 
      acttcttac accttcaag tgtgtcttt tgtgagcag 
      gtattgagt ggtagtgac gaacccaaa ttattggag 
      tgtttgcat aatgcttgt ggtaactgc attggtaac 
      aagctttac gaagagttg gagaaaact actgttgaa 
      tgtccacag tgcaatata gtatgtgag ccaagtaaa 
      ataatcgaa aatcagttt ttgctagag ctagcaagt 
      aacgaagac aactcgacc aaattagca gagttgaaa 
      tgtagcagt tgtagtgat gatgcaatt gctacaagt 
      tggtgtgtt gattgttca gagtacatt tgtgatagt 
      tgtgtacaa gcacatcaa agactaaaa ataactaaa 
      gaccatacc attaaaccg aaagaagag ggacttact 
      gacagccaa tcatctaca aataattct gcacctaat 
      ctattttgt cagattcat ccacaggag aaattgtca 
      ctattttgc gaaacgtgc gataaatta acttgtcgc 
      gattgccaa cttattgaa catagggaa cacaagtat 
      aaatttacc aatgaaatt gcgtcagag acaagaaaa 
      tttattgct gatatgttg aaggatgtt ggttacaag 
      agggcacta ttatgtagt gcgatgaag gtaatagac 
      gatcgtcaa aatttgatt gttgacaaa aaacaagca 
      cttgttaag gaaatcaca caattagtg gtcaagttg 
      acaaatacg attaatgtt agaggcaaa caattagtt 
      tcgaaatta accgaagtg tgtgatagt aaacaaaag 
      acgttacaa gagaaaaaa caagcttta gagcaactt 
      tcacgtatg acagatcac tgtatagag ttcacacaa 
      catgcttta gataacggc agtgatatg gctctatta 
      tacagtaaa aaacaagtc accgatcac ttacgtcga 
      atcaaatgt aaacgagcc gatattcca aatccagaa 
      ataccggta cgaattcat ctggctttg gataaattt 
      ccagatctt gttaaagtc atgtcaaca atcggtcaa 
      attgtggtt gatggtaga atttaccct tcacagtcc 
      gtctcaccc tcatctcaa ttacctcca catggttct 
      cccagtcct ggtcattcc agaccaccc caacaacaa 
      tcacccatc aatcaattg ctccaacaa tcacctcct 
      taccaacaa caaccatca gggttccaa caatcaccc 
      agattaccc ccagcatat ggacagttt aacaatggg 
      ccgcagtat ccacaaaat caaaatatt agcccacaa 
      tccaatatg ggagcaaga gtaatgggt ccaccaatg 
      gcacaacag atttcatta gcccaacaa ttatcagcc 
      aatcgtatg caaatgcca cggcaaatg gcgagacct 
      ttactacct cgaatgcca ccaaattct ggcggttta 
      aaccaacag caggtctcg tcatcaact cacccacaa 
      aatatgtta tctgatcgc agcaatttg cgatgttta 
      ttacaacca gcaagtaat tcacctgtt tatattcaa 
      acaggacct gctcaatat caagcagtc caaccacat 
      atggcccaa caataccaa actcgtttt cctaaccaa 
      tcacaacaa tcgtttcgt caacaaccg ccaatgaat 
      ccaatgatg atgaaccaa aatcgtcca caaagacag 
      tttcaaatg caaggtggt tccgccaga tggcacatt 
      ccacaacaa caaatagcc aataataat aacatgcaa 
      ggaggaaat cgtaatatg atcttaccg acaattaat 
      gataatttt aaaattacc ctaaaacaa cacaaccaa 
      tcagagaat agaaatcta ccagcgact gttacctca 
      accattcct aaaacacca agtcctaat catattcaa 
      ggtcgaagt gatactgag agaagtctt gataaattt 
      tgtgaagat tcggttaag gatttaatg gcgactatt 
      gcaaaattg gattcgaat ggtgtgcaa gttgtagct 
      gaaggaaga cagaaaact ggaggttca ccacatgtt 
      gatagttca acaggtgat gcaggacct tcaggcaag 
      attaaaagt gatccaatg gaaacaggc aaagatgac 
      ccgaatgag gattggtgt gctgtgtgt atggatgga 
      ggagaattg gtctgttgt gataaatgt ccgaaggtt 
      tttcatcag tactgtcac attccgaat ttgagtgtc 
      gaggaaaat gatacatgg cagtgtttg ttgtgcaca 
      aactttgcc gacttatcc gatgatatt ttaaatgag 
      aaaaaagaa ggagaactt tgttataaa gacaagaaa 
      attgctgaa cgtatagtt ttggaatta tattgtcag 
      tatgatcct agtttaccg ttccgggaa gttatcggg 
      cctgagaat gaggagtat catgcaatt atcaaaacc 
      ccgattgct cttgattca attcgtcag aaattaaac 
      tggaattcg ccggaacat tacacaact atggagcag 
      ttaatcaaa gacgtgcgt ttaatgttt aaaaatgca 
      tacacatat aatcctgag gattctcaa gtatacgcc 
      gatgctaaa gtcttagag aaattcttt gacgaacag 
      ttggaaaaa tttctgcca gaatacgct tatgaacat 
      ttcgatgat gatgatgat attcagccc ccaaataaa 
      aagtatcgg cgcataata agtgactga
    mRNA sequence
    • >TC007718 RA
      agtgaagtt tgagaggtt gatgtgttt tctggaaaa 
      gcgtcccca aaatattgg aaaaaaaaa gtaaataaa 
      agtgtaaat aatgcaaaa aacgggtga agacgatgt 
      tagtaaatg gtagccaaa cggccctaa tcaaggtgg 
      tgattaaaa tttcaaagg cgctccccc aacatcatc 
      aatttttca tttttaatt ggttctaat attgtccac 
      gatggctga atcaaatat ttattacaa cgagggcaa 
      ttatatgaa tcagcaaga cgctaccac caaggatgt 
      gattgattc agttcagaa tctgaatga gcagagtga 
      aatgatttg tgatgattc tgtaacaga aacagtgga 
      agaatcgaa ggatgaagt taaagaagt tgctgaagg 
      cactgaaga acatgtaga gagtccaac aactgggga 
      aacttctta caccttcaa gtgtgtctt ttgtgagca 
      ggtattgag tggtagtga cgaacccaa attattgga 
      gtgtttgca taatgcttg tggtaactg cattggtaa 
      caagcttta cgaagagtt ggagaaaac tactgttga 
      atgtccaca gtgcaatat agtatgtga gccaagtaa 
      aataatcga aaatcagtt tttgctaga gctagcaag 
      taacgaaga caactcgac caaattagc agagttgaa 
      atgtagcag ttgtagtga tgatgcaat tgctacaag 
      ttggtgtgt tgattgttc agagtacat ttgtgatag 
      ttgtgtaca agcacatca aagactaaa aataactaa 
      agaccatac cattaaacc gaaagaaga gggacttac 
      tgacagcca atcatctac aaataattc tgcacctaa 
      tctattttg tcagattca tccacagga gaaattgtc 
      actattttg cgaaacgtg cgataaatt aacttgtcg 
      cgattgcca acttattga acataggga acacaagta 
      taaatttac caatgaaat tgcgtcaga gacaagaaa 
      atttattgc tgatatgtt gaaggatgt tggttacaa 
      gagggcact attatgtag tgcgatgaa ggtaataga 
      cgatcgtca aaatttgat tgttgacaa aaaacaagc 
      acttgttaa ggaaatcac acaattagt ggtcaagtt 
      gacaaatac gattaatgt tagaggcaa acaattagt 
      ttcgaaatt aaccgaagt gtgtgatag taaacaaaa 
      gacgttaca agagaaaaa acaagcttt agagcaact 
      ttcacgtat gacagatca ctgtataga gttcacaca 
      acatgcttt agataacgg cagtgatat ggctctatt 
      atacagtaa aaaacaagt caccgatca cttacgtcg 
      aatcaaatg taaacgagc cgatattcc aaatccaga 
      aataccggt acgaattca tctggcttt ggataaatt 
      tccagatct tgttaaagt catgtcaac aatcggtca 
      aattgtggt tgatggtag aatttaccc ttcacagtc 
      cgtctcacc ctcatctca attacctcc acatggttc 
      tcccagtcc tggtcattc cagaccacc ccaacaaca 
      atcacccat caatcaatt gctccaaca atcacctcc 
      ttaccaaca acaaccatc agggttcca acaatcacc 
      cagattacc cccagcata tggacagtt taacaatgg 
      gccgcagta tccacaaaa tcaaaatat tagcccaca 
      atccaatat gggagcaag agtaatggg tccaccaat 
      ggcacaaca gatttcatt agcccaaca attatcagc 
      caatcgtat gcaaatgcc acggcaaat ggcgagacc 
      tttactacc tcgaatgcc accaaattc tggcggttt 
      aaaccaaca gcaggtctc gtcatcaac tcacccaca 
      aaatatgtt atctgatcg cagcaattt gcgatgttt 
      attacaacc agcaagtaa ttcacctgt ttatattca 
      aacaggacc tgctcaata tcaagcagt ccaaccaca 
      tatggccca acaatacca aactcgttt tcctaacca 
      atcacaaca atcgtttcg tcaacaacc gccaatgaa 
      tccaatgat gatgaacca aaatcgtcc acaaagaca 
      gtttcaaat gcaaggtgg ttccgccag atggcacat 
      tccacaaca acaaatagc caataataa taacatgca 
      aggaggaaa tcgtaatat gatcttacc gacaattaa 
      tgataattt taaaattac cctaaaaca acacaacca 
      atcagagaa tagaaatct accagcgac tgttacctc 
      aaccattcc taaaacacc aagtcctaa tcatattca 
      aggtcgaag tgatactga gagaagtct tgataaatt 
      ttgtgaaga ttcggttaa ggatttaat ggcgactat 
      tgcaaaatt ggattcgaa tggtgtgca agttgtagc 
      tgaaggaag acagaaaac tggaggttc accacatgt 
      tgatagttc aacaggtga tgcaggacc ttcaggcaa 
      gattaaaag tgatccaat ggaaacagg caaagatga 
      cccgaatga ggattggtg tgctgtgtg tatggatgg 
      aggagaatt ggtctgttg tgataaatg tccgaaggt 
      ttttcatca gtactgtca cattccgaa tttgagtgt 
      cgaggaaaa tgatacatg gcagtgttt gttgtgcac 
      aaactttgc cgacttatc cgatgatat tttaaatga 
      gaaaaaaga aggagaact ttgttataa agacaagaa 
      aattgctga acgtatagt tttggaatt atattgtca 
      gtatgatcc tagtttacc gttccggga agttatcgg 
      gcctgagaa tgaggagta tcatgcaat tatcaaaac 
      cccgattgc tcttgattc aattcgtca gaaattaaa 
      ctggaattc gccggaaca ttacacaac tatggagca 
      gttaatcaa agacgtgcg tttaatgtt taaaaatgc 
      atacacata taatcctga ggattctca agtatacgc 
      cgatgctaa agtcttaga gaaattctt tgacgaaca 
      gttggaaaa atttctgcc agaatacgc ttatgaaca 
      tttcgatga tgatgatga tattcagcc cccaaataa 
      aaagtatcg gcgcataat aagtgactg acaaattgt 
      tcaaggagc gaataatac agtatattt ctctctcta 
      atgcagaat gaaaactat ttttaatta aggaggagt 
      aagatattt atacagggt gattacaaa cgttttaac 
      ttctaatac agagattct gagttcaac taaattatt 
      ttcaaaatg gctgagttt tatacaata aaaataggt 
      ggttgccat ttggcgcta gtttctcat cgttccgga 
      agttcagcc attttgaaa ataacttag tagttctta 
      aaatggtcg attttgaag ctatgttat gttatagta 
      gaagaatca ccctgtaaa gtaaatacc agggtgcaa 
      atcgagtaa actgtttaa atgttggca agatgttag 
      atctttata ccgttttag cgattttat tgttagtgt 
      acgttttat ttaattggg tttttgtta ttacaaaga 
      actgtcatt tttagtgta ttttcacgt tattgatgt 
      tattagagg tcactgtca tatgttatt aaataaagc 
      taaatatga ccatgtcta tctttgacg tttgattgc 
      ttcgaaata tcgaatata tcaaaaaaa tgtacctag 
      tatatgtat attgagaaa acaacaaac acaagataa 
      atatttatc tattttgtt attgtattg attttctga 
      taatcaatt tgtaagcaa gctgtataa aattaaaat 
      aaacacgtt aatgccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY