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Gene information
General information
TC007717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 104279..121557
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007717
    iBeetle screen iB_01252

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: slightly above 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially not present # of remaining segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 8

    wings shortened - Number of animals showing the phenotype: 8

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007717 RA
      MGSREPMAW RKHDHLMLM DKNKPKMPV SSSGSGDTL 
      RQRHVPLYG RLVPEDQLG MGHMSNPPA DIQAMQARI 
      PHTFRDSSA APLRKLSVD LIKTYKHIN EVYYAKKKR 
      RAAQPQEDT SHKKERKLY NDGYDDDNH DYIVKNGEK 
      FLDRYEIDS LIGKGSFGQ VVKAYDHVE QCHVAIKII 
      KNKKPFLNQ AQIEVKLLE MMNRADAEN KYYIVKLKR 
      HFMWRNHLC LVFELLSYN LYDLLRNTN FRGVSLNLT 
      RKFAQQLCT ALLFLSTPE LNIIHCDLK PENILLCNP 
      KRSAIKIVD FGSSCQLGQ RIYQYIQSR FYRSPEVLL 
      GIPYDLAID MWSLGCILV EMHTGEPLF SGANEIDQM 
      NKIVEVLGM PPKSLLDQA NKTRKFFEK LPEGRYVLK 
      KAKDGKKYK LPGTRRLHD ILGVDSGGP GGRRAGEPG 
      HSTGEYLKF KDLILRMLD YDPKSRITS YYALQHNFF 
      KRTTDECTS TPNSNSSSP AVASMDMSS LTNSSSSQH 
      GLNLVGRSR ELSNYTTME CDPSPSRHP HRQQQRYHQ 
      PYGVTSLQY APYPGVPMT DGSAPNKTT SNGPQPPKS 
      TSTNERDDS PMVGVCVQH NSVAIH
    CDS sequence
    • >TC007717 RA
      atgggatcg agggagccc atggcgtgg cgcaagcac 
      gaccatctc atgctcatg gacaaaaac aaacccaaa 
      atgccggta tcatcgagt ggtagtggg gacaccctt 
      cgacagcgt catgtgccc ttatatgga cgtttggta 
      cccgaagat cagctgggt atgggtcat atgtccaat 
      ccaccagct gatattcaa gcaatgcaa gcacgaata 
      ccacacact tttagggat tcgtcggcg gcaccctta 
      cgcaaactc agcgtggac ctcatcaag acctacaag 
      cacatcaac gaggtgtat tatgcgaag aagaaacga 
      cgagcggcg caaccccaa gaagacacc tctcataaa 
      aaagagaga aaactatac aatgacggt tacgacgac 
      gataatcac gattatatc gtgaagaat ggtgaaaag 
      ttcctcgac cgatacgaa atcgactca cttatagga 
      aaaggatca ttcggacag gtcgttaag gcctacgat 
      cacgtggaa caatgtcac gtggctatc aaaattatc 
      aagaacaaa aaaccgttt ctcaatcag gcacagatc 
      gaggtgaaa ttgttggag atgatgaac agagcggat 
      gccgaaaat aagtattat atagttaaa ttaaaacgt 
      cattttatg tggcgaaac cacctgtgc ttagtgttc 
      gaactgctt tcgtataat ctatatgat ctgttgcga 
      aatacaaac ttccgcggt gtctcattg aatctcacg 
      agaaagttt gcccagcaa ctgtgcacg gctctattg 
      ttcctctca acgcccgaa ctcaacatc atccattgc 
      gatctgaaa ccggaaaat atacttttg tgcaatccg 
      aaacgatcg gctattaaa attgtcgat tttggaagt 
      tcttgccag cttggacag aggatatac caatacata 
      caatcaagg ttttataga tcacccgaa gtcctacta 
      ggtataccg tacgatcta gcgatagat atgtggtcg 
      ttgggttgt atcctagtc gaaatgcat acaggggaa 
      ccacttttc agtggtgcc aacgaaatc gatcagatg 
      aataaaatc gttgaagtg ttgggaatg ccgccgaaa 
      agtctactc gatcaggcc aacaagacg cgaaagttt 
      ttcgaaaaa ctgccagag gggagatac gtgttgaaa 
      aaggcgaaa gacggcaaa aagtacaaa ttgcctgga 
      acgcgtcga ctgcacgac attctcggt gtcgatagt 
      ggtggtcct ggagggcga agggccggc gaaccaggt 
      cattccact ggcgaatat ctcaagttc aaggatctg 
      attttacgc atgctcgat tacgatcct aaatctagg 
      ataacatcg tattacgct ctccaacat aattttttc 
      aaacgcacg acggacgaa tgtacttcg acgcctaat 
      agtaacagt tccagtccg gccgttgcc tcaatggat 
      atgtcttct ctgacgaat agttcgagt agtcaacac 
      gggttaaat ttggtggga cggtctcga gagctgtca 
      aattacacg acaatggaa tgcgatcca tctccttca 
      cgacatcct caccgtcag cagcagcgt taccaccag 
      ccctacgga gtgacgtca ttacagtac gccccatat 
      ccaggggtt cccatgacc gacggcagt gcccccaac 
      aaaacaacc agcaatggg ccacagcca cctaaatca 
      acatcaaca aatgaaaga gacgacagt ccgatggtt 
      ggggtttgc gttcagcac aattcggtc gcaatacat 
      tga
    mRNA sequence
    • >TC007717 RA
      gacgaatca actttacac ttgaaaaat gcgtaaaaa 
      gctaattca ataacgatt ttgaatttt aattcgcaa 
      cactagaga ctgttggtg tcatagccc atttgatta 
      gaaatttaa tttaatgca tgtcatttg tctgaagca 
      gcaggaaat tggcaagtc catgattca aagaagaat 
      aaaaaggta agcaacaaa tttaatatt tcagctaca 
      agtattgcg aaaatatta attagggta ttaaaaaat 
      gtgaaaaga taaacattt aatttttat ttcaataaa 
      gattttttt acaagaagt tttttattg aaaatcccg 
      taaaaaatc tttactcta agaaaagtt attgtctac 
      ggatctgat accactgta attttggca aaaaaaatt 
      gcaaaactg gttaattcc aaaaaagtg gtgacaagt 
      ggcgctgcc gtgtcaatt tcccaaact taaaacccc 
      gccgaatcg ttcgtgtgt attcgttgt agcgttcgg 
      cttgtagct gtcagtgcc tgcgcccaa aaacagttg 
      ccatgttgc ttgttgtgt agggtgtgc ttgtgataa 
      aaatcggcc aagtctctc cttgcagca aaagtgcgt 
      tgtctcgcg tgcccaccc ggtgtatgt gcattagtg 
      tgtgccccc ttgttggcg gtggtagtg ttggaggtg 
      cgggtgtgt ggtgaaaca aaccaacag agcacaatt 
      ccaagtggt gttgtttgg tggtggtgg tggtggttg 
      tggcaggca gcgtgcttt gcacgcaca tttgttgtt 
      atatgatgg gggcccgcc gctgaggcg cccgaggcc 
      ggcctggcc ttggagcga ggttactgg ccccgagcc 
      acgcgggat tgtttacca tgtgctaga ggtgtcgcg 
      agtggtgcc attatgctg agccccaac aactgctga 
      cacagaggg cgtacaaca aggctcaga gctacgttg 
      atgggatcg agggagccc atggcgtgg cgcaagcac 
      gaccatctc atgctcatg gacaaaaac aaacccaaa 
      atgccggta tcatcgagt ggtagtggg gacaccctt 
      cgacagcgt catgtgccc ttatatgga cgtttggta 
      cccgaagat cagctgggt atgggtcat atgtccaat 
      ccaccagct gatattcaa gcaatgcaa gcacgaata 
      ccacacact tttagggat tcgtcggcg gcaccctta 
      cgcaaactc agcgtggac ctcatcaag acctacaag 
      cacatcaac gaggtgtat tatgcgaag aagaaacga 
      cgagcggcg caaccccaa gaagacacc tctcataaa 
      aaagagaga aaactatac aatgacggt tacgacgac 
      gataatcac gattatatc gtgaagaat ggtgaaaag 
      ttcctcgac cgatacgaa atcgactca cttatagga 
      aaaggatca ttcggacag gtcgttaag gcctacgat 
      cacgtggaa caatgtcac gtggctatc aaaattatc 
      aagaacaaa aaaccgttt ctcaatcag gcacagatc 
      gaggtgaaa ttgttggag atgatgaac agagcggat 
      gccgaaaat aagtattat atagttaaa ttaaaacgt 
      cattttatg tggcgaaac cacctgtgc ttagtgttc 
      gaactgctt tcgtataat ctatatgat ctgttgcga 
      aatacaaac ttccgcggt gtctcattg aatctcacg 
      agaaagttt gcccagcaa ctgtgcacg gctctattg 
      ttcctctca acgcccgaa ctcaacatc atccattgc 
      gatctgaaa ccggaaaat atacttttg tgcaatccg 
      aaacgatcg gctattaaa attgtcgat tttggaagt 
      tcttgccag cttggacag aggatatac caatacata 
      caatcaagg ttttataga tcacccgaa gtcctacta 
      ggtataccg tacgatcta gcgatagat atgtggtcg 
      ttgggttgt atcctagtc gaaatgcat acaggggaa 
      ccacttttc agtggtgcc aacgaaatc gatcagatg 
      aataaaatc gttgaagtg ttgggaatg ccgccgaaa 
      agtctactc gatcaggcc aacaagacg cgaaagttt 
      ttcgaaaaa ctgccagag gggagatac gtgttgaaa 
      aaggcgaaa gacggcaaa aagtacaaa ttgcctgga 
      acgcgtcga ctgcacgac attctcggt gtcgatagt 
      ggtggtcct ggagggcga agggccggc gaaccaggt 
      cattccact ggcgaatat ctcaagttc aaggatctg 
      attttacgc atgctcgat tacgatcct aaatctagg 
      ataacatcg tattacgct ctccaacat aattttttc 
      aaacgcacg acggacgaa tgtacttcg acgcctaat 
      agtaacagt tccagtccg gccgttgcc tcaatggat 
      atgtcttct ctgacgaat agttcgagt agtcaacac 
      gggttaaat ttggtggga cggtctcga gagctgtca 
      aattacacg acaatggaa tgcgatcca tctccttca 
      cgacatcct caccgtcag cagcagcgt taccaccag 
      ccctacgga gtgacgtca ttacagtac gccccatat 
      ccaggggtt cccatgacc gacggcagt gcccccaac 
      aaaacaacc agcaatggg ccacagcca cctaaatca 
      acatcaaca aatgaaaga gacgacagt ccgatggtt 
      ggggtttgc gttcagcac aattcggtc gcaatacat 
      tgaggataa ttttgtgca ttgtgtttc ttctttata 
      taatttttt tttgttttt gtttatttt aagaaagtt 
      gtttagttg tggaggatt atagtgatg tgatttttt 
      tacaacaca cgtggacaa acaacagac ttggggcgc 
      attgaagct ctgttgtgt tttgacttg aagaaagaa 
      acttgaaat ttttactag gaattaagt taaaaatga 
      ggataaagt aatttatgc ctagtattt aaatattaa 
      tgattaatt ctagcgagt aaccacata tgtatacat 
      gtttcctat gatttaccg ttaactgta tgcaagttg 
      gctctggtt gtttaacac cagatcatt tgttttgtt 
      ttttagttt atttttttg tcaatcaag tgtaactag 
      atttagtac agcagtatt gaaccaagt gattccgtt 
      tggttgtaa acatacctc aagaagaaa gaaaaaaaa 
      agtaatttc caagccagg gccatacca atccactac 
      cgcaagtac agtggtaaa tgtgttcaa aatataata 
      ccagtttgt ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY