Status
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Gene information
General information
TC031094
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007712
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 72395..76726
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031094
    iBeetle screen iB_01251
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031094 RA
      MPRNNSEYE PIGESSHQT LDLNLEDFQ CDLYGFTRS 
      STRTFFYHF LGIVFLGIP YLLSSWFSR LKSWRYRKC 
      SLKHANIVL IKDVHGNSD FVEIKTENA KLPNYGHVY 
      LRFFTYQHA KYVWIPHSE IFGTLDLLI PPQTVDSYV 
      NNTDGLFYN DYVDLLKLY GSNKIEIEV KSYWRLFVD 
      EVFNPFYVF QAFSMTLWC FDHYYIYAC CVFILTLFS 
      VITALRQTR KQSEALHDL VESSKCHNV RVLRRNLLS 
      ENVLQEADP SELVPGDLI VLPKANFVL PCDVVLLTG 
      QCIVNESVL TGESVPVTK TALHSSNEI YNPNTHKRH 
      TLFSGTFMI QSRYYGGED VLARVVTTG FNTTKGALV 
      KSILFPTPV GLQFYKDSL KFVLALFII AGAGTAYCL 
      YLYTHRKAE IREIVIRSL DVITIVVPP ALPAAMTVG 
      IVYSQSRLK KLKIFCISP PKINVCGKL KLACFDKTG 
      TLTQDGLDM HSIVPCVNG TFGQPITEC FDCHDDRLV 
      QAMATCHSL TQIAGELTG DPLDLSMFH FTKWILEEP 
      GDDETARFD MLAPTIVKP VDSCDTSEF PYQLGIIRQ 
      FPFSSTLQC MSVICKELS ARNMIAFTK GAPEKISAM 
      CQPQSLPED FHTQLSQLA TQGYRVIAL AWKQMPVKF 
      KWKEAQRVK RDIVECDLT FLGLLVMQN TLKPETTPI 
      IRLLHDAKI RTVMITGDN ILTAISVAR DCEMVKSHD 
      QIFIVETKS SESGDSPEL VLQNIDSAS THSISIDFD 
      FSHCHLAID GKTWTKIRS FYPDLVPSL LIRTTIFAR 
      FQPDQKTQV ITHLQSLDY VVSMVGDGA NDCGALKAA 
      HVGVSLSQA EASVAAPFT SAIQDISCI VHLMLEGRC 
      ALVTSFAVF KYMALYSLI QFTTVLILY RQNSVLGDV 
      QFLFIDLII TTSLAVTIG RQGPSGKLG VKRPPGSLV 
      AAPILIPIL LQVVLCVVV QVGSMLYLF QQEWFKPVP 
      SHTDHEVIE CWENTVLFS VSSFQYLIL AFVYSKGKP 
      FRQRLIRNF WFLLCAVFL TLFQIWLLV SPTRKVAEF 
      FEIMFLPHK AKEQIIFRY KLLLIPLGH LLAAVFIEV 
      AIADRTWLK KLLHCVTRK KVPKNKYKL LLQNNDIHL 
      NLLNFNN
    CDS sequence
    • >TC031094 RA
      atgccacgc aataattct gaatatgaa ccaattggt 
      gaatcctcc caccaaacc ctcgacttg aaccttgaa 
      gacttccag tgtgacctt tacggtttc accaggagc 
      tctacacgc acttttttc taccatttt ttaggaatc 
      gtattttta ggcattccc tacttactt tccagttgg 
      ttttcgcgc ttgaaatcg tggagatat agaaaatgt 
      agcttaaaa cacgcaaac attgtcttg attaaagat 
      gtgcatggg aatagcgat tttgtcgaa attaagact 
      gaaaatgcc aaattgcct aattacggg cacgtatat 
      ttaagattt ttcacgtat caacatgcg aaatacgtg 
      tggatcccc catagcgaa atttttgga actctagac 
      cttttaata cctccacaa acggttgat agttatgtt 
      aataatacg gacgggtta ttttataac gattatgtc 
      gatttgtta aagttatac gggtcaaat aaaatcgaa 
      attgaagtt aagtcctat tggagattg ttcgtagac 
      gaggttttt aacccattc tatgtcttc caagcattt 
      tccatgact ttgtggtgt tttgatcat tattacatc 
      tacgcatgt tgcgttttt atcctcact ttattttcg 
      gtgatcact gccttacga caaacacga aaacaaagc 
      gaggctttg catgactta gtcgaatcg tccaagtgc 
      cataacgta agagtgtta cgacgaaat cttctctca 
      gagaatgtc cttcaagag gccgaccct tcagaacta 
      gtacctgga gatttaatc gttcttccg aaagctaat 
      ttcgtatta ccctgtgat gtagtttta ctaactgga 
      caatgtatt gttaacgaa agtgtgcta actggtgaa 
      agcgttcca gtgactaaa acagccctg cattcaagc 
      aacgaaatc tacaatcct aatacgcac aagcgacat 
      acacttttt tcagggact tttatgatc caaagtcgg 
      tattatggg ggcgaggat gttttagca cgagttgta 
      acgactggt tttaatacg acaaagggg gccctggtc 
      aagagcatc ctttttcca acccccgtt ggcttacaa 
      ttctacaaa gacagtctt aaatttgtt ttagctctc 
      ttcatcata gcgggcgct ggaaccgcc tactgtctc 
      tatttgtac acccatcgt aaggccgaa attcgcgaa 
      attgtcatc agaagtctc gatgtgata acaattgtg 
      gtaccacct gcattaccg gctgctatg acagtcggt 
      atagtctac agtcagagt cgcttaaaa aaactgaaa 
      atcttttgt ataagtccg cctaaaatt aacgtttgc 
      ggtaaatta aagttggcg tgttttgat aaaacgggt 
      actttgact caagatggc cttgatatg cattcaatt 
      gttccctgt gtcaatggg acattcggt cagccaatc 
      acagagtgc tttgattgt catgacgac agacttgta 
      caagccatg gccacttgt cattcactc acacaaatc 
      gctggcgaa ttgactggt gatcctctc gatttgagt 
      atgtttcat tttacgaaa tggatattg gaagagcca 
      ggtgatgat gaaaccgct cggtttgat atgttggca 
      ccgactata gtaaagccg gttgacagt tgtgatacc 
      tcggagttt ccatatcag ttgggtata attcgccaa 
      tttccgttt tcctcaact ttacaatgc atgtctgtg 
      atttgtaaa gagttgagt gcacgaaat atgattgca 
      tttactaaa ggtgcgccc gagaaaatt tccgcgatg 
      tgtcaacca caaagtctt cccgaagat tttcatacg 
      caattatcc cagttggca acgcaaggc tatagagtt 
      atagccctg gcatggaaa caaatgccg gtcaagttc 
      aaatggaaa gaggcacaa agagttaag agagatatc 
      gtcgagtgc gatttaaca tttctcggg ctccttgta 
      atgcaaaat acgcttaaa ccggaaacg actccgatt 
      attagactt ttgcatgat gctaaaatt cgcacagtt 
      atgataaca ggagataat attttaacg gcgatttcc 
      gtagctcgt gattgcgaa atggtgaaa agtcacgac 
      caaattttc atagttgag acgaaaagt agtgaatcg 
      ggtgattcc cctgagtta gttttacaa aatattgat 
      tcggccagc acgcatagt atttcaatc gatttcgat 
      ttttcgcac tgccattta gctattgat ggtaaaact 
      tggacgaaa attcgctcg ttttatcct gatcttgtt 
      ccaagtctt ttaattcgg accacaatt tttgcgcgt 
      tttcaaccc gatcaaaaa acacaagtt attacacat 
      ttacaaagt ttggattat gtcgtttcg atggtggga 
      gacggcgct aatgactgt ggggcctta aaagctgca 
      cacgttggt gtttcattg tctcaagct gaggcaagt 
      gtggcggca ccttttacc tctgctatt caagatatt 
      tcatgtatt gttcacttg atgttggaa gggaggtgt 
      gcgttagtt accagtttt gccgttttc aagtacatg 
      gcgctgtat agcttgatc caattcacc acggtttta 
      attttgtat aggcaaaat tcagtcttg ggagacgtc 
      caattcctt ttcatcgac ctaataatc acgacaagt 
      ttggctgtg accattggt aggcaaggt ccttcgggt 
      aaattaggt gtgaaaaga ccgccaggg tctctggtt 
      gctgcccca atccttatt cctattttg ttgcaagtt 
      gttctttgt gtagttgtc caagttggt tcaatgttg 
      tatttgttt caacaagaa tggttcaaa cctgtaccc 
      agtcatacc gaccatgaa gtaatcgag tgttgggaa 
      aataccgtc cttttttca gtctcgtct ttccagtat 
      cttatttta gcgtttgtc tattcaaaa ggaaagcct 
      tttcgtcaa agactgatt aggaacttt tggttttta 
      ctttgtgca gtttttctc actctcttt caaatttgg 
      ttgttggta tcgccaact cgaaaagtc gccgaattt 
      ttcgaaatc atgtttttg ccgcacaaa gccaaagaa 
      caaattatt tttagatac aaattgttg ttaattcca 
      cttggacat ttactagcc gctgtgttt attgaggtg 
      gccatagcc gaccgaact tggttaaaa aaacttttg 
      cattgcgtc acacgtaaa aaagtccca aaaaacaag 
      tacaaactg ctgttacaa aataacgac attcacctc 
      aatttatta aattttaat aattga
    mRNA sequence
    • >TC031094 RA
      ggcaacgca ggaatgaat cacttccct gtttttgtt 
      gctgacatg tgtataatg agataaaat cagcttacc 
      taatagttt ttgtatccc ttgtgtgtt aaaaacaat 
      gtgacaatg ccacgcaat aattctgaa tatgaacca 
      attggtgaa tcctcccac caaaccctc gacttgaac 
      cttgaagac ttccagtgt gacctttac ggtttcacc 
      aggagctct acacgcact tttttctac catttttta 
      ggaatcgta tttttaggc attccctac ttactttcc 
      agttggttt tcgcgcttg aaatcgtgg agatataga 
      aaatgtagc ttaaaacac gcaaacatt gtcttgatt 
      aaagatgtg catgggaat agcgatttt gtcgaaatt 
      aagactgaa aatgccaaa ttgcctaat tacgggcac 
      gtatattta agatttttc acgtatcaa catgcgaaa 
      tacgtgtgg atcccccat agcgaaatt tttggaact 
      ctagacctt ttaatacct ccacaaacg gttgatagt 
      tatgttaat aatacggac gggttattt tataacgat 
      tatgtcgat ttgttaaag ttatacggg tcaaataaa 
      atcgaaatt gaagttaag tcctattgg agattgttc 
      gtagacgag gtttttaac ccattctat gtcttccaa 
      gcattttcc atgactttg tggtgtttt gatcattat 
      tacatctac gcatgttgc gtttttatc ctcacttta 
      ttttcggtg atcactgcc ttacgacaa acacgaaaa 
      caaagcgag gctttgcat gacttagtc gaatcgtcc 
      aagtgccat aacgtaaga gtgttacga cgaaatctt 
      ctctcagag aatgtcctt caagaggcc gacccttca 
      gaactagta cctggagat ttaatcgtt cttccgaaa 
      gctaatttc gtattaccc tgtgatgta gttttacta 
      actggacaa tgtattgtt aacgaaagt gtgctaact 
      ggtgaaagc gttccagtg actaaaaca gccctgcat 
      tcaagcaac gaaatctac aatcctaat acgcacaag 
      cgacataca cttttttca gggactttt atgatccaa 
      agtcggtat tatgggggc gaggatgtt ttagcacga 
      gttgtaacg actggtttt aatacgaca aagggggcc 
      ctggtcaag agcatcctt tttccaacc cccgttggc 
      ttacaattc tacaaagac agtcttaaa tttgtttta 
      gctctcttc atcatagcg ggcgctgga accgcctac 
      tgtctctat ttgtacacc catcgtaag gccgaaatt 
      cgcgaaatt gtcatcaga agtctcgat gtgataaca 
      attgtggta ccacctgca ttaccggct gctatgaca 
      gtcggtata gtctacagt cagagtcgc ttaaaaaaa 
      ctgaaaatc ttttgtata agtccgcct aaaattaac 
      gtttgcggt aaattaaag ttggcgtgt tttgataaa 
      acgggtact ttgactcaa gatggcctt gatatgcat 
      tcaattgtt ccctgtgtc aatgggaca ttcggtcag 
      ccaatcaca gagtgcttt gattgtcat gacgacaga 
      cttgtacaa gccatggcc acttgtcat tcactcaca 
      caaatcgct ggcgaattg actggtgat cctctcgat 
      ttgagtatg tttcatttt acgaaatgg atattggaa 
      gagccaggt gatgatgaa accgctcgg tttgatatg 
      ttggcaccg actatagta aagccggtt gacagttgt 
      gatacctcg gagtttcca tatcagttg ggtataatt 
      cgccaattt ccgttttcc tcaacttta caatgcatg 
      tctgtgatt tgtaaagag ttgagtgca cgaaatatg 
      attgcattt actaaaggt gcgcccgag aaaatttcc 
      gcgatgtgt caaccacaa agtcttccc gaagatttt 
      catacgcaa ttatcccag ttggcaacg caaggctat 
      agagttata gccctggca tggaaacaa atgccggtc 
      aagttcaaa tggaaagag gcacaaaga gttaagaga 
      gatatcgtc gagtgcgat ttaacattt ctcgggctc 
      cttgtaatg caaaatacg cttaaaccg gaaacgact 
      ccgattatt agacttttg catgatgct aaaattcgc 
      acagttatg ataacagga gataatatt ttaacggcg 
      atttccgta gctcgtgat tgcgaaatg gtgaaaagt 
      cacgaccaa attttcata gttgagacg aaaagtagt 
      gaatcgggt gattcccct gagttagtt ttacaaaat 
      attgattcg gccagcacg catagtatt tcaatcgat 
      ttcgatttt tcgcactgc catttagct attgatggt 
      aaaacttgg acgaaaatt cgctcgttt tatcctgat 
      cttgttcca agtctttta attcggacc acaattttt 
      gcgcgtttt caacccgat caaaaaaca caagttatt 
      acacattta caaagtttg gattatgtc gtttcgatg 
      gtgggagac ggcgctaat gactgtggg gccttaaaa 
      gctgcacac gttggtgtt tcattgtct caagctgag 
      gcaagtgtg gcggcacct tttacctct gctattcaa 
      gatatttca tgtattgtt cacttgatg ttggaaggg 
      aggtgtgcg ttagttacc agttttgcc gttttcaag 
      tacatggcg ctgtatagc ttgatccaa ttcaccacg 
      gttttaatt ttgtatagg caaaattca gtcttggga 
      gacgtccaa ttccttttc atcgaccta ataatcacg 
      acaagtttg gctgtgacc attggtagg caaggtcct 
      tcgggtaaa ttaggtgtg aaaagaccg ccagggtct 
      ctggttgct gccccaatc cttattcct attttgttg 
      caagttgtt ctttgtgta gttgtccaa gttggttca 
      atgttgtat ttgtttcaa caagaatgg ttcaaacct 
      gtacccagt cataccgac catgaagta atcgagtgt 
      tgggaaaat accgtcctt ttttcagtc tcgtctttc 
      cagtatctt attttagcg tttgtctat tcaaaagga 
      aagcctttt cgtcaaaga ctgattagg aacttttgg 
      tttttactt tgtgcagtt tttctcact ctctttcaa 
      atttggttg ttggtatcg ccaactcga aaagtcgcc 
      gaatttttc gaaatcatg tttttgccg cacaaagcc 
      aaagaacaa attattttt agatacaaa ttgttgtta 
      attccactt ggacattta ctagccgct gtgtttatt 
      gaggtggcc atagccgac cgaacttgg ttaaaaaaa 
      cttttgcat tgcgtcaca cgtaaaaaa gtcccaaaa 
      aacaagtac aaactgctg ttacaaaat aacgacatt 
      cacctcaat ttattaaat tttaataat tgatagtat 
      tatgtttgt attttgatt gcaaataaa aatctaatc 
      ataat