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Gene information
General information
TC007702 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1176..3533
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01249

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC007702 RA
    CDS sequence
    • >TC007702 RA
      atggcaagt gttgatgac gacaacgga tgggtggct 
      gatttagaa agcgcttta ctggaagaa tgttcagtt 
      gctgatatt tactgtatt tgcaaaggc aaaccttta 
      cctgacaat ttacgagct gaagtctgg cagatatgt 
      ctcgacgta agagacaag gggaaccaa ctcgatcac 
      tttaacgaa atctatgat ttgcctaat caatctgtg 
      ctacgagaa gactgtcga aatttcgtt tcaaaactc 
      ggcaatgac gaagaggat aaattatcc gttgtatcc 
      gatttagaa tcgatttta acattttat tgcaaaagt 
      cgcaacttg acttatatt aaaaacaac ggctggatt 
      gagcttctg ctccctttg ctaacactg aaagtgaat 
      cgttcaacc acttacaac ctgtttgag gcaattaga 
      gacacttac gtgcctcaa aattgtcac acaaatgac 
      ggcaacgct ttccatatt ttgcgtctt ttattgctc 
      tatcacgat ccagaattg tgttcattt cttgacact 
      aaacgtatc acacctgat aaatattgt ctgacatgg 
      ttccagtca cttttcgca gccacttgt aatttacaa 
      gtcgtcata aacatgtgg gactattat tttcaacag 
      tctgacccg tttttcatt tttttcctc tcgttaatc 
      atggtaata aacgcacgc gagcaaatc atttcaatg 
      aaaagcgaa tctcgagat gtaattatt tcgaatttg 
      acgaacatg ccgtgtgct cttgaggct gaagacgtt 
      ggtgatttt tgttccttg gcccaatat tatgccctt 
      aaaacaccc gttagtttt cggaccgat ttaatgcaa 
      aacttgtat aatggcaca aatgatgct gtagtctct 
      caagcgtta tgtctccca gtttccgta agcgaacta 
      gttgaaaat acgtccaat gagagtgga aatatagac 
      gctgtaaga tttttccta gttgattgt cgacctgtg 
      gaacagtac aatgccggt catttacct acagctttt 
      cacttggac tgtaactta atgcttcaa gaaccaagt 
      gcttttaat actgctgtt caaggctta ctttcggcg 
      caaaaacaa gctctggca cataattct acagcaggt 
      ggtgaacat ttgtgtttt ttgggatca ggtcgtaac 
      gaagaagat caatacact catatggtt gtggcttct 
      ttcctacag aagcacact caatacgtt tcacttcta 
      acaggaggt tattgtgct cttcatcat tacttttcg 
      gatgattta gagttttta caagatcat aatcccaaa 
      ctttgcatt gtgtgtggc ccaatgcaa caacaacaa 
      caacaacag caaggtcaa gtcaaggga caatcgacc 
      gatttgttt gggaaaatt tcagcagca atgaaatcg 
      aaatcggcc gaagttaaa gaaaaactt atcgattat 
      atcgttaat cccaacaat agtcccgtg caagagaga 
      catgttagc agtagcgac aaaatcggg aaaaggtac 
      cgaaatgtg gcacctgtt tttagtata gatgatgat 
      catgaaaat gctaccacc aataacatg gaagatgca 
      actaatgaa gaatgtcgc gaattggta caaatacaa 
      tcatggttg aaacatccc gaagttatt gaacatttt 
      ccgtgtcag gaggtgaaa atcaatggt tttatgtac 
      aaaagtcac ttgattgtg actaacagt gagcttata 
      gtacttaga gaaatgccc gataggaaa ggtggcatt 
      gctgaagtt attgtaaaa cgccccctg tcggcgatt 
      gttaaaata acagcgagg aaacgtcat cctgaactt 
      atcactttc aagtatggc gtgccagat ggtgagaat 
      ttaattata tctgacatg gatcgattt cttataccc 
      aatgccgat aaagctaca aaagttgta tctgaacag 
      attttaaag cagttgaag aataaggag taa
    mRNA sequence
    • >TC007702 RA
      aaactttct ggtacccat ggctgccaa tccctctta 
      tcatctgct gttttgaat gtcactttt tgacacgtt 
      gatgtggta ttttcctct ttttcgtcg cttattttt 
      tctttaata tgtgaaaat ggcaagtgt tgatgacga 
      caacggatg ggtggctga tttagaaag cgctttact 
      ggaagaatg ttcagttgc tgatattta ctgtatttg 
      caaaggcaa acctttacc tgacaattt acgagctga 
      agtctggca gatatgtct cgacgtaag agacaaggg 
      gaaccaact cgatcactt taacgaaat ctatgattt 
      gcctaatca atctgtgct acgagaaga ctgtcgaaa 
      tttcgtttc aaaactcgg caatgacga agaggataa 
      attatccgt tgtatccga tttagaatc gattttaac 
      attttattg caaaagtcg caacttgac ttatattaa 
      aaacaacgg ctggattga gcttctgct ccctttgct 
      aacactgaa agtgaatcg ttcaaccac ttacaacct 
      gtttgaggc aattagaga cacttacgt gcctcaaaa 
      ttgtcacac aaatgacgg caacgcttt ccatatttt 
      gcgtctttt attgctcta tcacgatcc agaattgtg 
      ttcatttct tgacactaa acgtatcac acctgataa 
      atattgtct gacatggtt ccagtcact tttcgcagc 
      cacttgtaa tttacaagt cgtcataaa catgtggga 
      ctattattt tcaacagtc tgacccgtt tttcatttt 
      tttcctctc gttaatcat ggtaataaa cgcacgcga 
      gcaaatcat ttcaatgaa aagcgaatc tcgagatgt 
      aattatttc gaatttgac gaacatgcc gtgtgctct 
      tgaggctga agacgttgg tgatttttg ttccttggc 
      ccaatatta tgcccttaa aacacccgt tagttttcg 
      gaccgattt aatgcaaaa cttgtataa tggcacaaa 
      tgatgctgt agtctctca agcgttatg tctcccagt 
      ttccgtaag cgaactagt tgaaaatac gtccaatga 
      gagtggaaa tatagacgc tgtaagatt tttcctagt 
      tgattgtcg acctgtgga acagtacaa tgccggtca 
      tttacctac agcttttca cttggactg taacttaat 
      gcttcaaga accaagtgc ttttaatac tgctgttca 
      aggcttact ttcggcgca aaaacaagc tctggcaca 
      taattctac agcaggtgg tgaacattt gtgtttttt 
      gggatcagg tcgtaacga agaagatca atacactca 
      tatggttgt ggcttcttt cctacagaa gcacactca 
      atacgtttc acttctaac aggaggtta ttgtgctct 
      tcatcatta cttttcgga tgatttaga gtttttaca 
      agatcataa tcccaaact ttgcattgt gtgtggccc 
      aatgcaaca acaacaaca acaacagca aggtcaagt 
      caagggaca atcgaccga tttgtttgg gaaaatttc 
      agcagcaat gaaatcgaa atcggccga agttaaaga 
      aaaacttat cgattatat cgttaatcc caacaatag 
      tcccgtgca agagagaca tgttagcag tagcgacaa 
      aatcgggaa aaggtaccg aaatgtggc acctgtttt 
      tagtataga tgatgatca tgaaaatgc taccaccaa 
      taacatgga agatgcaac taatgaaga atgtcgcga 
      attggtaca aatacaatc atggttgaa acatcccga 
      agttattga acattttcc gtgtcagga ggtgaaaat 
      caatggttt tatgtacaa aagtcactt gattgtgac 
      taacagtga gcttatagt acttagaga aatgcccga 
      taggaaagg tggcattgc tgaagttat tgtaaaacg 
      ccccctgtc ggcgattgt taaaataac agcgaggaa 
      acgtcatcc tgaacttat cactttcaa gtatggcgt 
      gccagatgg tgagaattt aattatatc tgacatgga 
      tcgatttct tatacccaa tgccgataa agctacaaa 
      agttgtatc tgaacagat tttaaagca gttgaagaa 
      taaggagta atttggaaa tttaaatag agttacaca 
      tatttattg ttactgtag atattttat tata

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY