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Gene information
General information
TC007702 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1176..3533
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007702
    iBeetle screen iB_01249

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007702 RA
      MASVDDDNG WVADLESAL LEECSVADI YCICKGKPL 
PDNLRAEVW QICLDVRDK GNQLDHFNE IYDLPNQSV 
LREDCRNFV SKLGNDEED KLSVVSDLE SILTFYCKS 
RNLTYIKNN GWIELLLPL LTLKVNRST TYNLFEAIR 
DTYVPQNCH TNDGNAFHI LRLLLLYHD PELCSFLDT 
KRITPDKYC LTWFQSLFA ATCNLQVVI NMWDYYFQQ 
SDPFFIFFL SLIMVINAR EQIISMKSE SRDVIISNL 
TNMPCALEA EDVGDFCSL AQYYALKTP VSFRTDLMQ 
NLYNGTNDA VVSQALCLP VSVSELVEN TSNESGNID 
AVRFFLVDC RPVEQYNAG HLPTAFHLD CNLMLQEPS 
AFNTAVQGL LSAQKQALA HNSTAGGEH LCFLGSGRN 
EEDQYTHMV VASFLQKHT QYVSLLTGG YCALHHYFS 
DDLEFLQDH NPKLCIVCG PMQQQQQQQ QGQVKGQST 
DLFGKISAA MKSKSAEVK EKLIDYIVN PNNSPVQER 
HVSSSDKIG KRYRNVAPV FSIDDDHEN ATTNNMEDA 
TNEECRELV QIQSWLKHP EVIEHFPCQ EVKINGFMY 
KSHLIVTNS ELIVLREMP DRKGGIAEV IVKRPLSAI 
VKITARKRH PELITFKYG VPDGENLII SDMDRFLIP 
NADKATKVV SEQILKQLK NKE
    CDS sequence
    • >TC007702 RA
      atggcaagt gttgatgac gacaacgga tgggtggct 
gatttagaa agcgcttta ctggaagaa tgttcagtt 
gctgatatt tactgtatt tgcaaaggc aaaccttta 
cctgacaat ttacgagct gaagtctgg cagatatgt 
ctcgacgta agagacaag gggaaccaa ctcgatcac 
tttaacgaa atctatgat ttgcctaat caatctgtg 
ctacgagaa gactgtcga aatttcgtt tcaaaactc 
ggcaatgac gaagaggat aaattatcc gttgtatcc 
gatttagaa tcgatttta acattttat tgcaaaagt 
cgcaacttg acttatatt aaaaacaac ggctggatt 
gagcttctg ctccctttg ctaacactg aaagtgaat 
cgttcaacc acttacaac ctgtttgag gcaattaga 
gacacttac gtgcctcaa aattgtcac acaaatgac 
ggcaacgct ttccatatt ttgcgtctt ttattgctc 
tatcacgat ccagaattg tgttcattt cttgacact 
aaacgtatc acacctgat aaatattgt ctgacatgg 
ttccagtca cttttcgca gccacttgt aatttacaa 
gtcgtcata aacatgtgg gactattat tttcaacag 
tctgacccg tttttcatt tttttcctc tcgttaatc 
atggtaata aacgcacgc gagcaaatc atttcaatg 
aaaagcgaa tctcgagat gtaattatt tcgaatttg 
acgaacatg ccgtgtgct cttgaggct gaagacgtt 
ggtgatttt tgttccttg gcccaatat tatgccctt 
aaaacaccc gttagtttt cggaccgat ttaatgcaa 
aacttgtat aatggcaca aatgatgct gtagtctct 
caagcgtta tgtctccca gtttccgta agcgaacta 
gttgaaaat acgtccaat gagagtgga aatatagac 
gctgtaaga tttttccta gttgattgt cgacctgtg 
gaacagtac aatgccggt catttacct acagctttt 
cacttggac tgtaactta atgcttcaa gaaccaagt 
gcttttaat actgctgtt caaggctta ctttcggcg 
caaaaacaa gctctggca cataattct acagcaggt 
ggtgaacat ttgtgtttt ttgggatca ggtcgtaac 
gaagaagat caatacact catatggtt gtggcttct 
ttcctacag aagcacact caatacgtt tcacttcta 
acaggaggt tattgtgct cttcatcat tacttttcg 
gatgattta gagttttta caagatcat aatcccaaa 
ctttgcatt gtgtgtggc ccaatgcaa caacaacaa 
caacaacag caaggtcaa gtcaaggga caatcgacc 
gatttgttt gggaaaatt tcagcagca atgaaatcg 
aaatcggcc gaagttaaa gaaaaactt atcgattat 
atcgttaat cccaacaat agtcccgtg caagagaga 
catgttagc agtagcgac aaaatcggg aaaaggtac 
cgaaatgtg gcacctgtt tttagtata gatgatgat 
catgaaaat gctaccacc aataacatg gaagatgca 
actaatgaa gaatgtcgc gaattggta caaatacaa 
tcatggttg aaacatccc gaagttatt gaacatttt 
ccgtgtcag gaggtgaaa atcaatggt tttatgtac 
aaaagtcac ttgattgtg actaacagt gagcttata 
gtacttaga gaaatgccc gataggaaa ggtggcatt 
gctgaagtt attgtaaaa cgccccctg tcggcgatt 
gttaaaata acagcgagg aaacgtcat cctgaactt 
atcactttc aagtatggc gtgccagat ggtgagaat 
ttaattata tctgacatg gatcgattt cttataccc 
aatgccgat aaagctaca aaagttgta tctgaacag 
attttaaag cagttgaag aataaggag taa
    mRNA sequence
    • >TC007702 RA
      aaactttct ggtacccat ggctgccaa tccctctta 
tcatctgct gttttgaat gtcactttt tgacacgtt 
gatgtggta ttttcctct ttttcgtcg cttattttt 
tctttaata tgtgaaaat ggcaagtgt tgatgacga 
caacggatg ggtggctga tttagaaag cgctttact 
ggaagaatg ttcagttgc tgatattta ctgtatttg 
caaaggcaa acctttacc tgacaattt acgagctga 
agtctggca gatatgtct cgacgtaag agacaaggg 
gaaccaact cgatcactt taacgaaat ctatgattt 
gcctaatca atctgtgct acgagaaga ctgtcgaaa 
tttcgtttc aaaactcgg caatgacga agaggataa 
attatccgt tgtatccga tttagaatc gattttaac 
attttattg caaaagtcg caacttgac ttatattaa 
aaacaacgg ctggattga gcttctgct ccctttgct 
aacactgaa agtgaatcg ttcaaccac ttacaacct 
gtttgaggc aattagaga cacttacgt gcctcaaaa 
ttgtcacac aaatgacgg caacgcttt ccatatttt 
gcgtctttt attgctcta tcacgatcc agaattgtg 
ttcatttct tgacactaa acgtatcac acctgataa 
atattgtct gacatggtt ccagtcact tttcgcagc 
cacttgtaa tttacaagt cgtcataaa catgtggga 
ctattattt tcaacagtc tgacccgtt tttcatttt 
tttcctctc gttaatcat ggtaataaa cgcacgcga 
gcaaatcat ttcaatgaa aagcgaatc tcgagatgt 
aattatttc gaatttgac gaacatgcc gtgtgctct 
tgaggctga agacgttgg tgatttttg ttccttggc 
ccaatatta tgcccttaa aacacccgt tagttttcg 
gaccgattt aatgcaaaa cttgtataa tggcacaaa 
tgatgctgt agtctctca agcgttatg tctcccagt 
ttccgtaag cgaactagt tgaaaatac gtccaatga 
gagtggaaa tatagacgc tgtaagatt tttcctagt 
tgattgtcg acctgtgga acagtacaa tgccggtca 
tttacctac agcttttca cttggactg taacttaat 
gcttcaaga accaagtgc ttttaatac tgctgttca 
aggcttact ttcggcgca aaaacaagc tctggcaca 
taattctac agcaggtgg tgaacattt gtgtttttt 
gggatcagg tcgtaacga agaagatca atacactca 
tatggttgt ggcttcttt cctacagaa gcacactca 
atacgtttc acttctaac aggaggtta ttgtgctct 
tcatcatta cttttcgga tgatttaga gtttttaca 
agatcataa tcccaaact ttgcattgt gtgtggccc 
aatgcaaca acaacaaca acaacagca aggtcaagt 
caagggaca atcgaccga tttgtttgg gaaaatttc 
agcagcaat gaaatcgaa atcggccga agttaaaga 
aaaacttat cgattatat cgttaatcc caacaatag 
tcccgtgca agagagaca tgttagcag tagcgacaa 
aatcgggaa aaggtaccg aaatgtggc acctgtttt 
tagtataga tgatgatca tgaaaatgc taccaccaa 
taacatgga agatgcaac taatgaaga atgtcgcga 
attggtaca aatacaatc atggttgaa acatcccga 
agttattga acattttcc gtgtcagga ggtgaaaat 
caatggttt tatgtacaa aagtcactt gattgtgac 
taacagtga gcttatagt acttagaga aatgcccga 
taggaaagg tggcattgc tgaagttat tgtaaaacg 
ccccctgtc ggcgattgt taaaataac agcgaggaa 
acgtcatcc tgaacttat cactttcaa gtatggcgt 
gccagatgg tgagaattt aattatatc tgacatgga 
tcgatttct tatacccaa tgccgataa agctacaaa 
agttgtatc tgaacagat tttaaagca gttgaagaa 
taaggagta atttggaaa tttaaatag agttacaca 
tatttattg ttactgtag atattttat tata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY