| General information | |||
| TC007702 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1176..3533 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007702 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01249 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC007702 RA
MASVDDDNG WVADLESAL LEECSVADI YCICKGKPL PDNLRAEVW QICLDVRDK GNQLDHFNE IYDLPNQSV LREDCRNFV SKLGNDEED KLSVVSDLE SILTFYCKS RNLTYIKNN GWIELLLPL LTLKVNRST TYNLFEAIR DTYVPQNCH TNDGNAFHI LRLLLLYHD PELCSFLDT KRITPDKYC LTWFQSLFA ATCNLQVVI NMWDYYFQQ SDPFFIFFL SLIMVINAR EQIISMKSE SRDVIISNL TNMPCALEA EDVGDFCSL AQYYALKTP VSFRTDLMQ NLYNGTNDA VVSQALCLP VSVSELVEN TSNESGNID AVRFFLVDC RPVEQYNAG HLPTAFHLD CNLMLQEPS AFNTAVQGL LSAQKQALA HNSTAGGEH LCFLGSGRN EEDQYTHMV VASFLQKHT QYVSLLTGG YCALHHYFS DDLEFLQDH NPKLCIVCG PMQQQQQQQ QGQVKGQST DLFGKISAA MKSKSAEVK EKLIDYIVN PNNSPVQER HVSSSDKIG KRYRNVAPV FSIDDDHEN ATTNNMEDA TNEECRELV QIQSWLKHP EVIEHFPCQ EVKINGFMY KSHLIVTNS ELIVLREMP DRKGGIAEV IVKRPLSAI VKITARKRH PELITFKYG VPDGENLII SDMDRFLIP NADKATKVV SEQILKQLK NKE
- >TC007702 RA
atggcaagt gttgatgac gacaacgga tgggtggct gatttagaa agcgcttta ctggaagaa tgttcagtt gctgatatt tactgtatt tgcaaaggc aaaccttta cctgacaat ttacgagct gaagtctgg cagatatgt ctcgacgta agagacaag gggaaccaa ctcgatcac tttaacgaa atctatgat ttgcctaat caatctgtg ctacgagaa gactgtcga aatttcgtt tcaaaactc ggcaatgac gaagaggat aaattatcc gttgtatcc gatttagaa tcgatttta acattttat tgcaaaagt cgcaacttg acttatatt aaaaacaac ggctggatt gagcttctg ctccctttg ctaacactg aaagtgaat cgttcaacc acttacaac ctgtttgag gcaattaga gacacttac gtgcctcaa aattgtcac acaaatgac ggcaacgct ttccatatt ttgcgtctt ttattgctc tatcacgat ccagaattg tgttcattt cttgacact aaacgtatc acacctgat aaatattgt ctgacatgg ttccagtca cttttcgca gccacttgt aatttacaa gtcgtcata aacatgtgg gactattat tttcaacag tctgacccg tttttcatt tttttcctc tcgttaatc atggtaata aacgcacgc gagcaaatc atttcaatg aaaagcgaa tctcgagat gtaattatt tcgaatttg acgaacatg ccgtgtgct cttgaggct gaagacgtt ggtgatttt tgttccttg gcccaatat tatgccctt aaaacaccc gttagtttt cggaccgat ttaatgcaa aacttgtat aatggcaca aatgatgct gtagtctct caagcgtta tgtctccca gtttccgta agcgaacta gttgaaaat acgtccaat gagagtgga aatatagac gctgtaaga tttttccta gttgattgt cgacctgtg gaacagtac aatgccggt catttacct acagctttt cacttggac tgtaactta atgcttcaa gaaccaagt gcttttaat actgctgtt caaggctta ctttcggcg caaaaacaa gctctggca cataattct acagcaggt ggtgaacat ttgtgtttt ttgggatca ggtcgtaac gaagaagat caatacact catatggtt gtggcttct ttcctacag aagcacact caatacgtt tcacttcta acaggaggt tattgtgct cttcatcat tacttttcg gatgattta gagttttta caagatcat aatcccaaa ctttgcatt gtgtgtggc ccaatgcaa caacaacaa caacaacag caaggtcaa gtcaaggga caatcgacc gatttgttt gggaaaatt tcagcagca atgaaatcg aaatcggcc gaagttaaa gaaaaactt atcgattat atcgttaat cccaacaat agtcccgtg caagagaga catgttagc agtagcgac aaaatcggg aaaaggtac cgaaatgtg gcacctgtt tttagtata gatgatgat catgaaaat gctaccacc aataacatg gaagatgca actaatgaa gaatgtcgc gaattggta caaatacaa tcatggttg aaacatccc gaagttatt gaacatttt ccgtgtcag gaggtgaaa atcaatggt tttatgtac aaaagtcac ttgattgtg actaacagt gagcttata gtacttaga gaaatgccc gataggaaa ggtggcatt gctgaagtt attgtaaaa cgccccctg tcggcgatt gttaaaata acagcgagg aaacgtcat cctgaactt atcactttc aagtatggc gtgccagat ggtgagaat ttaattata tctgacatg gatcgattt cttataccc aatgccgat aaagctaca aaagttgta tctgaacag attttaaag cagttgaag aataaggag taa
- >TC007702 RA
aaactttct ggtacccat ggctgccaa tccctctta tcatctgct gttttgaat gtcactttt tgacacgtt gatgtggta ttttcctct ttttcgtcg cttattttt tctttaata tgtgaaaat ggcaagtgt tgatgacga caacggatg ggtggctga tttagaaag cgctttact ggaagaatg ttcagttgc tgatattta ctgtatttg caaaggcaa acctttacc tgacaattt acgagctga agtctggca gatatgtct cgacgtaag agacaaggg gaaccaact cgatcactt taacgaaat ctatgattt gcctaatca atctgtgct acgagaaga ctgtcgaaa tttcgtttc aaaactcgg caatgacga agaggataa attatccgt tgtatccga tttagaatc gattttaac attttattg caaaagtcg caacttgac ttatattaa aaacaacgg ctggattga gcttctgct ccctttgct aacactgaa agtgaatcg ttcaaccac ttacaacct gtttgaggc aattagaga cacttacgt gcctcaaaa ttgtcacac aaatgacgg caacgcttt ccatatttt gcgtctttt attgctcta tcacgatcc agaattgtg ttcatttct tgacactaa acgtatcac acctgataa atattgtct gacatggtt ccagtcact tttcgcagc cacttgtaa tttacaagt cgtcataaa catgtggga ctattattt tcaacagtc tgacccgtt tttcatttt tttcctctc gttaatcat ggtaataaa cgcacgcga gcaaatcat ttcaatgaa aagcgaatc tcgagatgt aattatttc gaatttgac gaacatgcc gtgtgctct tgaggctga agacgttgg tgatttttg ttccttggc ccaatatta tgcccttaa aacacccgt tagttttcg gaccgattt aatgcaaaa cttgtataa tggcacaaa tgatgctgt agtctctca agcgttatg tctcccagt ttccgtaag cgaactagt tgaaaatac gtccaatga gagtggaaa tatagacgc tgtaagatt tttcctagt tgattgtcg acctgtgga acagtacaa tgccggtca tttacctac agcttttca cttggactg taacttaat gcttcaaga accaagtgc ttttaatac tgctgttca aggcttact ttcggcgca aaaacaagc tctggcaca taattctac agcaggtgg tgaacattt gtgtttttt gggatcagg tcgtaacga agaagatca atacactca tatggttgt ggcttcttt cctacagaa gcacactca atacgtttc acttctaac aggaggtta ttgtgctct tcatcatta cttttcgga tgatttaga gtttttaca agatcataa tcccaaact ttgcattgt gtgtggccc aatgcaaca acaacaaca acaacagca aggtcaagt caagggaca atcgaccga tttgtttgg gaaaatttc agcagcaat gaaatcgaa atcggccga agttaaaga aaaacttat cgattatat cgttaatcc caacaatag tcccgtgca agagagaca tgttagcag tagcgacaa aatcgggaa aaggtaccg aaatgtggc acctgtttt tagtataga tgatgatca tgaaaatgc taccaccaa taacatgga agatgcaac taatgaaga atgtcgcga attggtaca aatacaatc atggttgaa acatcccga agttattga acattttcc gtgtcagga ggtgaaaat caatggttt tatgtacaa aagtcactt gattgtgac taacagtga gcttatagt acttagaga aatgcccga taggaaagg tggcattgc tgaagttat tgtaaaacg ccccctgtc ggcgattgt taaaataac agcgaggaa acgtcatcc tgaacttat cactttcaa gtatggcgt gccagatgg tgagaattt aattatatc tgacatgga tcgatttct tatacccaa tgccgataa agctacaaa agttgtatc tgaacagat tttaaagca gttgaagaa taaggagta atttggaaa tttaaatag agttacaca tatttattg ttactgtag atattttat tata
