Status
Please wait ...
Gene information
General information
TC007700 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 622884..627901
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007700
    iBeetle screen iB_01248
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007700 RA
      MPSLLEALE QKYGESSTE SEGSDDEGI SVAIFVPCK 
      SPRSIVPSL LVLNDCDIA TAGEKEALE AKCACVEEL 
      DLAKNKLKD WPEVFGILQ QMPRLKFVN LSFNELNTP 
      LKNVEVDRN LRWQQLRNL VLNSTYIDW ESVDQILDH 
      LPRLEELHL SMNDYNVVN LCKKNCDCG RIDNKTDNE 
      TKCFCPTID YRKKHKHGG IRKLHFNGN PIDKWREVC 
      KLGYAFPNL ESLVLADCP IESLDVNVE SDDEKCNRN 
      YERSESECE SGNNKESPH DSFRVLKFL NLNSTRLAT 
      WDDIERLSK FPALHCVRI QGCPLWESN EYTEHERRQ 
      LLIARLPNV EILNGGGVI GSDEREDAE RAFIRYYME 
      KPESDRPER YFELVSIHG KLDPLVNVD LRPEKRVKV 
      TFTCGSNSE VRSVDVYRS VSDLKTKLE AFAGFSAAK 
      MRLFYVDQD LRDIQGPEE MKYPHKQLY SYNIQSGDE 
      IIIDCKKKI NEK
    CDS sequence
    • >TC007700 RA
      atgccttcg ctcctcgaa gccctcgaa cagaaatac 
      ggcgaaagt tcaaccgag tcggaaggt tcagatgac 
      gaaggcatt tcagtggcc attttcgtg ccttgtaaa 
      tcccctcga agtatcgtt ccttcactt cttgtttta 
      aacgactgc gatattgca acagctggt gaaaaagaa 
      gctctggaa gcgaaatgt gcatgtgtt gaagaatta 
      gaccttgcc aaaaacaag ctcaaagac tggccagag 
      gtttttgga attttgcaa caaatgcca cgtttgaaa 
      ttcgtaaat ttgagcttc aatgagttg aacacgccc 
      ttgaagaac gtggaagtc gacaggaat ttgcgatgg 
      cagcagttg aggaattta gtcctgaat tcgacttac 
      atcgactgg gaaagtgtc gatcaaatc cttgatcat 
      ttgccccgt ttggaagaa ttgcacttg agtatgaac 
      gattataac gttgtgaat ttatgcaag aaaaattgt 
      gattgtggc cgaattgat aataaaacg gacaatgaa 
      actaaatgt ttttgtccc acaattgat taccgaaag 
      aaacacaaa catggaggt atccgaaaa ctgcacttt 
      aacggaaat ccgattgat aaatggaga gaagtgtgt 
      aaattgggt tacgcattt cctaattta gaatcactt 
      gtacttgcc gattgtccg attgagtcg cttgatgtt 
      aatgtagaa agcgatgat gagaaatgt aataggaat 
      tatgagcga tcggaaagt gaatgtgag tcggggaat 
      aacaaagaa tcgccacat gatagtttc agagttttg 
      aaattttta aatttaaat tcgacaaga cttgccact 
      tgggatgat atcgaacga ttgtccaag tttccagct 
      cttcactgc gtcagaata cagggttgt ccgttgtgg 
      gagagcaac gagtacacc gaacacgaa cgccgccaa 
      ctcctcatt gcccgcctc ccaaatgtg gagatttta 
      aacgggggc ggcgttatc ggaagtgac gaaagggaa 
      gatgcagaa agagctttc attcgctat tacatggaa 
      aaaccggaa agtgaccga ccggaaaga tatttcgaa 
      ttagtttcg attcatggc aaattagat ccgttagta 
      aacgttgat ttaagaccg gaaaaacga gtcaaagtc 
      acatttact tgtggttca aatagcgaa gttcgatcg 
      gtggatgtt tatcggtca gtgagcgat ttgaaaacg 
      aaattagag gcgtttgct gggttttca gccgccaaa 
      atgaggttg ttttacgtc gatcaagac ttgagggat 
      attcaaggc ccggaggaa atgaagtat ccgcataag 
      cagctctac agttacaac atacagtca ggcgatgag 
      attataatt gattgcaag aagaaaatt aacgaaaag 
      taa
    mRNA sequence
    • >TC007700 RA
      ctcggaaaa tttaaataa acattgtcc ggccgtata 
      aagcacaaa aatgccttc gctcctcga agccctcga 
      acagaaata cggcgaaag ttcaaccga gtcggaagg 
      ttcagatga cgaaggcat ttcagtggc cattttcgt 
      gccttgtaa atcccctcg aagtatcgt tccttcact 
      tcttgtttt aaacgactg cgatattgc aacagctgg 
      tgaaaaaga agctctgga agcgaaatg tgcatgtgt 
      tgaagaatt agaccttgc caaaaacaa gctcaaaga 
      ctggccaga ggtttttgg aattttgca acaaatgcc 
      acgtttgaa attcgtaaa tttgagctt caatgagtt 
      gaacacgcc cttgaagaa cgtggaagt cgacaggaa 
      tttgcgatg gcagcagtt gaggaattt agtcctgaa 
      ttcgactta catcgactg ggaaagtgt cgatcaaat 
      ccttgatca tttgccccg tttggaaga attgcactt 
      gagtatgaa cgattataa cgttgtgaa tttatgcaa 
      gaaaaattg tgattgtgg ccgaattga taataaaac 
      ggacaatga aactaaatg tttttgtcc cacaattga 
      ttaccgaaa gaaacacaa acatggagg tatccgaaa 
      actgcactt taacggaaa tccgattga taaatggag 
      agaagtgtg taaattggg ttacgcatt tcctaattt 
      agaatcact tgtacttgc cgattgtcc gattgagtc 
      gcttgatgt taatgtaga aagcgatga tgagaaatg 
      taataggaa ttatgagcg atcggaaag tgaatgtga 
      gtcggggaa taacaaaga atcgccaca tgatagttt 
      cagagtttt gaaattttt aaatttaaa ttcgacaag 
      acttgccac ttgggatga tatcgaacg attgtccaa 
      gtttccagc tcttcactg cgtcagaat acagggttg 
      tccgttgtg ggagagcaa cgagtacac cgaacacga 
      acgccgcca actcctcat tgcccgcct cccaaatgt 
      ggagatttt aaacggggg cggcgttat cggaagtga 
      cgaaaggga agatgcaga aagagcttt cattcgcta 
      ttacatgga aaaaccgga aagtgaccg accggaaag 
      atatttcga attagtttc gattcatgg caaattaga 
      tccgttagt aaacgttga tttaagacc ggaaaaacg 
      agtcaaagt cacatttac ttgtggttc aaatagcga 
      agttcgatc ggtggatgt ttatcggtc agtgagcga 
      tttgaaaac gaaattaga ggcgtttgc tgggttttc 
      agccgccaa aatgaggtt gttttacgt cgatcaaga 
      cttgaggga tattcaagg cccggagga aatgaagta 
      tccgcataa gcagctcta cagttacaa catacagtc 
      aggcgatga gattataat tgattgcaa gaagaaaat 
      taacgaaaa gtaaagtag tggccataa gatggatgc 
      gtatattgt tttagaaac taattaagt tttcaatta 
      aatattttt ctacgtaac aacgtcttc attgtctag 
      cagctgtag tctagttaa gatttgtga tatttgctg 
      tttgttcag caaaactat tgtgaaaat gaaactgcc 
      atgtagttt ttatttttc ccattaatt cgtactaaa 
      actggtgtg tggtaaata ttgtgaatt a