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Gene information
General information
TC007689 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 851787..872823
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007689
    iBeetle screen iB_01245

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 44.44% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    remarks: entire repetition development delayed, later than schedule, hours 60
    Pupal morphology
    remarks: entire repetition development delayed, later than schedule, hours 60
    Adult morphology
    remarks: entire repetition development delayed, later than schedule, hours 60
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007689 RA
      MDNVVEQRC TRAANMPIN AEHIGITGV AGRFPNCAN 
      VEELKDALL GGVDLLSNS HDRWSCESL ETSSRLGII 
      HDFEYFDAA FFGINPREA NTSDPRLRK ILEVTYEAV 
      LDAGMNPTD LRGKNVGVF LAVTQNDIS DLNYRGGII 
      ARSAAIMAN VVSFSFDFK GPSLAVNTA CSSGFYALT 
      TAVDCILAD RCDMAIVGA VQHQFDPAE SMELIKLGV 
      LHKEGVCRS FDANRKGYV KSEAIAALV LQKIDDSKR 
      MYATLAGFG CNVEGFKRD GMAHPSHKM QMAMFQQVF 
      KHFNLDPLD VNYVETHCT GTRVGDVEE VAAIDAFYT 
      KGRKTPVLI GSIKSNIGH TETTSGLVS LTKVVIAIR 
      EGVIPGNLH FKTPDPEMK GICEGRLKV IDKNMPIPE 
      GLISVNVFG FGGANAMVV LRPFKDRKQ VINSLSHRL 
      VHVSGRSEE GVKVMLDAA IKHKANAQF LSLLDNIFH 
      KPIDNHNYR GYAILSNDS HMQVTQTST RKRPVWFVY 
      SGFGAQYPG MGKDMMKNE VFRNTIKVC ANALKPHGV 
      DLEDVIMNG TDDTFNNLI NTFTAITAI SVALTDVLA 
      SLNITPAGI IGHSLGEVA CAYADGLIT AEQAVLIAH 
      ARSYATTSS NLIPGAMAA VGLSAEECA KLLPEGVYI 
      ACDNSDDNV TVSGPDFAV KDFLNQLDV RGVFNRLIS 
      NANYPLHSP HLEPAGQKM YSSVKKVLP EVKARTSRW 
      LPSAVPESE WNTDLGKYN SAEYQFHNY TNRVYFRQL 
      LKHVPKDAI VVEVAPRGL LQAILRRGL DKNVTLVPL 
      LKPGVDNVD FFLSSVGKY YNAGGPLDV TQLYKPAQF 
      PVSIDTPMI NNLVRWDHR VKWHKLRFN PRSYFGKRI 
      DIDMTDQSN HYLEGHIAD GRMMLPAAG YIVLVWQTY 
      ARMLEIPFD SLPVVFENA KTLRPVILA ENAITSVVV 
      NLFRSTGKF EICLENDED HPLAIGQIR AAADTIDEE 
      FLDYSSLPR PSQYKLKIS SEDFYNCMH LRGFDYDGL 
      FQGVQSCDL DGRSATLKW NDNWVAFID SMTQFILSI 
      EATGHSLPT VYEKIVINP QEHLKSISA NQTVTLNYN 
      KHLKAAKCG GVELRSLTV TNVKRRVAD VEPCLRKYT 
      FVPNNDTKK EADSFHHAL DVASQIVIE NTTEYLNIN 
      IGEIKSDAA IQILRTMIE PVNLKKTTF HQLNSIDDN 
      MEAKYDMLI AQEITKDQS DLLKDYLRR SRFVFAKVQ 
      NTDLKDVDL IYQHVTKTG IYVLLRRPQ DLPENYTVV 
      MANNNNYNW VKDLKMAVK KYRSSQSRI YLINEDFNS 
      GIVGWFNTI RHEQYCPNI RLFLIRSYK EVEPFALTS 
      KFYSRQLKK DLTVNVYQD GAWGTYRRL PLEPFQPRL 
      YADACLMKD NSGVKWVEK SPYVTKCEI MVHFASLSS 
      WDLAKITGK GGDAEYELL GLEYSGSYY ALFVQTHLS 
      PKDSILIHN SGTDVSMAA IALATNKNC TVYIVINSS 
      NRDVLRKFP KIQENNIIY DSEGSPFYE TLMTKTNGK 
      GVNTIFNCN SIELLKLSK KCIAKNGRF IEFGNSLGT 
      FLETESLME NNCSLYNIN VNDVFDETP EILDIIAKD 
      IDRYVKEGV VVPLKYSVY GKDDIEIAL KNLEDKDYV 
      KKCLIKIKD NGGFFSKPE NRILAFPRT YMDPSKCYI 
      VIGGLGGFG FELTKWLIE RGASKIVIN SRREVRSGY 
      HASCLQKWN NQKITVLTN TSDTATLEG AESLINFAT 
      NLAPVGGIF NAALHVKDG FMTDLGPED FAAVAGSKI 
      FSAQNMDIL SRKLCKKLD YFLMFSSIA SSIGNAVQS 
      NYAFVNSAV ERLCETRKA EGLPALALE WGPLADVGI 
      LVRLKMTNP ILETVRQKL SSCLRVVDQ YMQQSEPVV 
      TSCAYPDQS GEVSEKKTL AELVAGLFG IKNLEEIED 
      NTTLLELGM DSLLGFEVK HIITKESGL DVSVNDIRN 
      MTFEKIKEM SAPA
    CDS sequence
    • >TC007689 RA
      atggataat gtggttgag caaaggtgc actagagca 
      gcaaacatg ccgatcaat gctgaacat atcggaatc 
      accggcgtc gccggccgc ttccccaac tgcgccaac 
      gtggaggag ctcaaagac gccctcctc ggcggcgtg 
      gacctcctc tccaacagc cacgaccgc tggtcctgc 
      gagagcttg gaaacctcc tcccgtctc ggcataatc 
      cacgacttc gaatacttc gacgccgct ttcttcggc 
      atcaacccc agggaggcc aacacgagc gacccccgt 
      ctccgcaaa atcctggag gtcacctac gaggcggtg 
      ctggacgct ggcatgaac cccacggac ctccgtggc 
      aaaaacgtg ggcgttttc ctcgcagtc acccagaac 
      gacatctcc gacttgaac taccgtggc gggatcatc 
      gccaggtcg gctgccatc atggccaac gtcgtttcg 
      ttctccttt gacttcaag ggcccgagt ctggctgtg 
      aacacggcg tgttcgagc gggttttac gcactgacc 
      actgccgta gattgcatt ctggccgat aggtgcgat 
      atggccatt gttggggct gtccagcac cagttcgac 
      ccggctgag agcatggag ctgatcaag ctgggggtg 
      ctgcacaag gagggagtt tgcaggtcg tttgatgcc 
      aataggaag ggttatgtc aagtcggag gcgattgct 
      gctctagtt ttgcaaaag atcgatgat agtaaaagg 
      atgtatgcg actctggct gggttcggg tgcaatgtg 
      gaagggttt aaaagagac gggatggca cacccttca 
      cataagatg caaatggcg atgttccag caagttttt 
      aagcatttt aatcttgat cccttggat gtgaactac 
      gtggagacg cattgcacg ggaactagg gttggggat 
      gtggaagaa gtggcggcg attgatgcg ttttatacc 
      aaagggcgc aaaacgcca gtgttgatt ggctccatt 
      aagagtaat attggacat acggagacc acgtcaggg 
      ttggtgtcc ttgactaag gttgtgatt gccatcagg 
      gaaggggtg attcccggg aatttgcac tttaaaacg 
      cccgatcca gaaatgaag ggcatttgt gaagggagg 
      ttaaaggta attgacaaa aacatgcca atccccgaa 
      ggcctcatc tccgtcaac gtcttcggt ttcggcggc 
      gccaacgcc atggtggtc ctgcgcccc ttcaaagac 
      cgcaaacaa gtcataaac agcctcagc caccgcctg 
      gtgcacgtc tcaggccgc tcagaagaa ggcgtcaaa 
      gtgatgcta gacgctgcc attaaacac aaagccaat 
      gcacaattc ctttccctt ctggacaac atcttccac 
      aaacccatt gacaaccac aactaccgt ggctatgca 
      attttatcc aacgatagt cacatgcaa gtcacccaa 
      acctcgacc aggaaacgt ccagtctgg ttcgtctat 
      tctggcttt ggggcccag tatccaggc atgggcaag 
      gatatgatg aaaaacgag gtgtttaga aacaccatt 
      aaagtgtgt gctaatgct ttgaaacca catggtgtg 
      gacttggag gacgttatc atgaatggg accgatgat 
      acgttcaat aatttgatc aatactttc actgcaatc 
      actgcgatc tctgtggct ttgactgat gttttggcc 
      agtttgaat atcacgccg gctgggatt atcggacac 
      tcgttgggg gaagttgct tgtgcctac gccgatggt 
      ttgatcacc gccgaacaa gccgtcttg atcgcccat 
      gcccgaagt tacgccaca acttcgtcc aacttaatc 
      cctggtgcc atggcagct gtcggcctc tctgccgaa 
      gaatgcgcc aaactcctc cctgaagga gtttacatc 
      gcttgtgac aactccgac gacaacgtc acagtctct 
      gggcctgac ttcgcagtc aaagacttt ttgaaccag 
      ctcgacgtc cgtggcgtt ttcaaccga ctgatcagc 
      aacgccaac taccctctc cacagcccc cacctcgag 
      ccagccggc caaaaaatg tacagttcc gtgaagaaa 
      gtcctcccg gaagtcaaa gcccggact tcacggtgg 
      ctgccttcg gcggttccg gaaagcgaa tggaacaca 
      gatttgggg aaatacaat tcggccgaa taccagttc 
      cacaattac accaaccgg gtttatttc agacagttg 
      ttgaagcat gtgcctaag gatgccatt gtggtggag 
      gttgcacct agaggtctc cttcaggcg attttgcgc 
      cgcgggctt gataagaac gtgacgctt gttcctttg 
      cttaagcca ggtgtggat aatgttgat ttcttcttg 
      tcgagtgtt gggaagtac tataatgct ggagggccg 
      ctggatgtg acacagttg tacaagcca gcacagttc 
      cctgtcagt attgacacg ccgatgatc aataatttg 
      gtcaggtgg gaccatagg gtgaagtgg cacaagttg 
      aggttcaat ccgaggagt tacttcggg aagaggatt 
      gatattgat atgacggac caaagcaac cattatctg 
      gagggacat attgccgat gggagaatg atgttacca 
      gcggctggg tatattgtt ctagtctgg caaacctac 
      gcccgcatg ttggaaatc cccttcgac tccttgcct 
      gtggtcttc gaaaacgcc aaaaccctt cgtcccgtc 
      atcctcgcc gagaacgcc atcacaagc gtcgtcgtc 
      aacctgttc cgctcgacc ggcaaattc gaaatttgc 
      cttgaaaac gacgaagac caccctctg gccatcggc 
      cagatccgc gcagcggcc gacaccatc gacgaggag 
      ttcctggac tactcctcc ctgccacgg ccaagccaa 
      tacaagctg aaaatcagc agcgaagac ttctacaac 
      tgcatgcat ttgcgtggc ttcgactac gacggcctg 
      ttccagggc gtgcaaagc tgtgatctc gacggccgt 
      tcagccact ttgaaatgg aacgacaac tgggtggcg 
      ttcattgac agcatgacc cagtttatc ctaagtatt 
      gaagccaca gggcacagt ctcccaaca gtgtacgaa 
      aaaatcgta atcaaccca caagagcac ctgaaatcg 
      atcagcgcc aaccagact gtgacgctc aactacaac 
      aagcatttg aaagctgcc aagtgtggt ggtgttgaa 
      ctgaggagt ctaactgtg accaatgtg aagagacga 
      gtggctgat gtggaaccg tgcttgcgg aaatacact 
      tttgtgccg aataacgac accaagaaa gaggcagat 
      tcgttccac cacgcccta gatgttgcc tcccaaatt 
      gtaatagaa aacaccacc gaatatttg aacattaac 
      attggagaa atcaaatca gacgctgct atacaaatt 
      ctccgaacg atgattgaa cccgtcaac ttgaaaaaa 
      accaccttc caccaactc aactcaatt gacgacaac 
      atggaggcg aagtacgac atgctaatc gcccaggaa 
      atcacaaag gatcagagt gacctcctc aaggactat 
      ctgaggcgt tcgcgtttt gttttcgcc aaagtgcaa 
      aacactgac ttgaaggac gtcgacttg atttaccaa 
      cacgtcaca aaaactggt atttatgtt ttactgaga 
      cgtccccaa gacttgcct gaaaattac acggttgtg 
      atggcaaac aacaacaac tacaattgg gtcaaagac 
      ttgaaaatg gcagtgaaa aaataccgt tcctcccaa 
      tcccgcatt tatttaatc aacgaagac ttcaattcg 
      ggaatagtt ggttggttt aatacaata agacacgaa 
      cagtactgt cccaacatt cgtttgttc ctcatcagg 
      agctacaaa gaggtggaa ccgttcgcc ctcacctca 
      aaattctac tcccggcag ttgaagaag gacctcacg 
      gtgaatgtg taccaggac ggggcttgg gggacgtac 
      cgccggttg cccctggag ccgttccag ccccggctg 
      tacgccgat gcctgtctt atgaaggat aacagtggg 
      gttaagtgg gtggagaag tcgccgtat gtcaccaag 
      tgcgagatt atggtgcat tttgcgagt ctgagtagt 
      tgggatttg gcgaaaatc acagggaag ggcggggat 
      gccgagtat gagttgctg gggttggag tattcgggg 
      agttactac gccctgttt gtgcaaacc cacctctcc 
      cccaaggac tcaatcctc atccacaac tccggcact 
      gacgtcagc atggccgct atcgccctc gccaccaac 
      aaaaactgc accgtttac atcgtcatc aactcctcc 
      aaccgggac gtgttacgc aaattcccc aaaatccag 
      gaaaacaac atcatctac gacagcgaa ggcagtccc 
      ttctacgaa accctcatg accaaaacg aacggcaaa 
      ggcgtcaac acaatcttc aactgcaac tcaatcgaa 
      ctcctcaaa ctctccaaa aaatgcatc gccaaaaac 
      ggccgcttc atcgagttc ggcaacagc ttaggcacg 
      ttcttggaa actgaaagt ttgatggaa aacaactgt 
      tccctgtac aacatcaat gtgaacgac gtcttcgac 
      gaaaccccc gagatcctc gacatcata gccaaggat 
      attgataga tatgtcaaa gagggcgtg gttgtgccg 
      ctcaaatac tcggtgtat ggcaaggat gacatcgag 
      atcgcgttg aagaacctg gaggataag gactacgtg 
      aagaagtgt ttgatcaag attaaggac aacggtggg 
      ttcttctcc aagccggag aaccgcatc ctggcgttc 
      ccgcgcact tacatggac cccagcaag tgctacatc 
      gtgatcggg ggcttgggt gggttcggt ttcgagttg 
      accaagtgg ctgattgaa cgtggtgcc agcaagatc 
      gtgatcaac tcgcgccgg gaggtgcgc tccggttac 
      cacgccagt tgcctgcag aagtggaac aaccagaag 
      atcacagtc ttgaccaac acctccgac actgcgacg 
      ctcgaaggc gccgaatcc ttaatcaac ttcgccacg 
      aacttggca cctgtcggt ggcatcttc aacgccgcg 
      ctgcacgtg aaggacggc ttcatgacc gacttgggg 
      cccgaggat ttcgcagcg gtggccgga tccaagatt 
      ttcagtgcc cagaacatg gatatcctg tcacggaaa 
      ctgtgcaag aaactcgac tatttcctc atgttctct 
      tccattgcg tcgagtatt ggaaacgcg gtacaatcc 
      aattacgcg ttcgtcaac tcggcggtt gagcgtctc 
      tgtgagacg cgcaaggct gaggggctg ccggcgctg 
      gcgttggaa tgggggccg ttggccgat gttggtatt 
      ttggtgcgc ttgaaaatg acgaacccg attctggag 
      actgtgagg cagaagttg agttcgtgt ctgagggtt 
      gttgaccag tatatgcag caaagtgag ccggttgtc 
      accagttgt gcgtatcca gatcagtcg ggggaagtt 
      agcgagaag aagacgctg gcggagttg gttgctggc 
      ttgtttggg atcaagaat ttggaggag attgaagat 
      aatacgact ttgttggag ttgggaatg gattcgttg 
      cttgggttt gaagtcaag catattatt accaaagag 
      tcgggcttg gatgttagt gtgaacgat atacgaaat 
      atgaccttc gagaagatt aaggaaatg tcggcacct 
      gcttaa
    mRNA sequence
    • >TC007689 RA
      ttcggttta aaaaatatt gacggtttt tttggaaag 
      aaattattt tgacttaat atttcgtta aaaaattaa 
      taattaata aataatttt tatggccgt ttgtacata 
      aaaatggat aatgtggtt gagcaaagg tgcactaga 
      gcagcaaac atgccgatc aatgctgaa catatcgga 
      atcaccggc gtcgccggc cgcttcccc aactgcgcc 
      aacgtggag gagctcaaa gacgccctc ctcggcggc 
      gtggacctc ctctccaac agccacgac cgctggtcc 
      tgcgagagc ttggaaacc tcctcccgt ctcggcata 
      atccacgac ttcgaatac ttcgacgcc gctttcttc 
      ggcatcaac cccagggag gccaacacg agcgacccc 
      cgtctccgc aaaatcctg gaggtcacc tacgaggcg 
      gtgctggac gctggcatg aaccccacg gacctccgt 
      ggcaaaaac gtgggcgtt ttcctcgca gtcacccag 
      aacgacatc tccgacttg aactaccgt ggcgggatc 
      atcgccagg tcggctgcc atcatggcc aacgtcgtt 
      tcgttctcc tttgacttc aagggcccg agtctggct 
      gtgaacacg gcgtgttcg agcgggttt tacgcactg 
      accactgcc gtagattgc attctggcc gataggtgc 
      gatatggcc attgttggg gctgtccag caccagttc 
      gacccggct gagagcatg gagctgatc aagctgggg 
      gtgctgcac aaggaggga gtttgcagg tcgtttgat 
      gccaatagg aagggttat gtcaagtcg gaggcgatt 
      gctgctcta gttttgcaa aagatcgat gatagtaaa 
      aggatgtat gcgactctg gctgggttc gggtgcaat 
      gtggaaggg tttaaaaga gacgggatg gcacaccct 
      tcacataag atgcaaatg gcgatgttc cagcaagtt 
      tttaagcat tttaatctt gatcccttg gatgtgaac 
      tacgtggag acgcattgc acgggaact agggttggg 
      gatgtggaa gaagtggcg gcgattgat gcgttttat 
      accaaaggg cgcaaaacg ccagtgttg attggctcc 
      attaagagt aatattgga catacggag accacgtca 
      gggttggtg tccttgact aaggttgtg attgccatc 
      agggaaggg gtgattccc gggaatttg cactttaaa 
      acgcccgat ccagaaatg aagggcatt tgtgaaggg 
      aggttaaag gtaattgac aaaaacatg ccaatcccc 
      gaaggcctc atctccgtc aacgtcttc ggtttcggc 
      ggcgccaac gccatggtg gtcctgcgc cccttcaaa 
      gaccgcaaa caagtcata aacagcctc agccaccgc 
      ctggtgcac gtctcaggc cgctcagaa gaaggcgtc 
      aaagtgatg ctagacgct gccattaaa cacaaagcc 
      aatgcacaa ttcctttcc cttctggac aacatcttc 
      cacaaaccc attgacaac cacaactac cgtggctat 
      gcaatttta tccaacgat agtcacatg caagtcacc 
      caaacctcg accaggaaa cgtccagtc tggttcgtc 
      tattctggc tttggggcc cagtatcca ggcatgggc 
      aaggatatg atgaaaaac gaggtgttt agaaacacc 
      attaaagtg tgtgctaat gctttgaaa ccacatggt 
      gtggacttg gaggacgtt atcatgaat gggaccgat 
      gatacgttc aataatttg atcaatact ttcactgca 
      atcactgcg atctctgtg gctttgact gatgttttg 
      gccagtttg aatatcacg ccggctggg attatcgga 
      cactcgttg ggggaagtt gcttgtgcc tacgccgat 
      ggtttgatc accgccgaa caagccgtc ttgatcgcc 
      catgcccga agttacgcc acaacttcg tccaactta 
      atccctggt gccatggca gctgtcggc ctctctgcc 
      gaagaatgc gccaaactc ctccctgaa ggagtttac 
      atcgcttgt gacaactcc gacgacaac gtcacagtc 
      tctgggcct gacttcgca gtcaaagac tttttgaac 
      cagctcgac gtccgtggc gttttcaac cgactgatc 
      agcaacgcc aactaccct ctccacagc ccccacctc 
      gagccagcc ggccaaaaa atgtacagt tccgtgaag 
      aaagtcctc ccggaagtc aaagcccgg acttcacgg 
      tggctgcct tcggcggtt ccggaaagc gaatggaac 
      acagatttg gggaaatac aattcggcc gaataccag 
      ttccacaat tacaccaac cgggtttat ttcagacag 
      ttgttgaag catgtgcct aaggatgcc attgtggtg 
      gaggttgca cctagaggt ctccttcag gcgattttg 
      cgccgcggg cttgataag aacgtgacg cttgttcct 
      ttgcttaag ccaggtgtg gataatgtt gatttcttc 
      ttgtcgagt gttgggaag tactataat gctggaggg 
      ccgctggat gtgacacag ttgtacaag ccagcacag 
      ttccctgtc agtattgac acgccgatg atcaataat 
      ttggtcagg tgggaccat agggtgaag tggcacaag 
      ttgaggttc aatccgagg agttacttc gggaagagg 
      attgatatt gatatgacg gaccaaagc aaccattat 
      ctggaggga catattgcc gatgggaga atgatgtta 
      ccagcggct gggtatatt gttctagtc tggcaaacc 
      tacgcccgc atgttggaa atccccttc gactccttg 
      cctgtggtc ttcgaaaac gccaaaacc cttcgtccc 
      gtcatcctc gccgagaac gccatcaca agcgtcgtc 
      gtcaacctg ttccgctcg accggcaaa ttcgaaatt 
      tgccttgaa aacgacgaa gaccaccct ctggccatc 
      ggccagatc cgcgcagcg gccgacacc atcgacgag 
      gagttcctg gactactcc tccctgcca cggccaagc 
      caatacaag ctgaaaatc agcagcgaa gacttctac 
      aactgcatg catttgcgt ggcttcgac tacgacggc 
      ctgttccag ggcgtgcaa agctgtgat ctcgacggc 
      cgttcagcc actttgaaa tggaacgac aactgggtg 
      gcgttcatt gacagcatg acccagttt atcctaagt 
      attgaagcc acagggcac agtctccca acagtgtac 
      gaaaaaatc gtaatcaac ccacaagag cacctgaaa 
      tcgatcagc gccaaccag actgtgacg ctcaactac 
      aacaagcat ttgaaagct gccaagtgt ggtggtgtt 
      gaactgagg agtctaact gtgaccaat gtgaagaga 
      cgagtggct gatgtggaa ccgtgcttg cggaaatac 
      acttttgtg ccgaataac gacaccaag aaagaggca 
      gattcgttc caccacgcc ctagatgtt gcctcccaa 
      attgtaata gaaaacacc accgaatat ttgaacatt 
      aacattgga gaaatcaaa tcagacgct gctatacaa 
      attctccga acgatgatt gaacccgtc aacttgaaa 
      aaaaccacc ttccaccaa ctcaactca attgacgac 
      aacatggag gcgaagtac gacatgcta atcgcccag 
      gaaatcaca aaggatcag agtgacctc ctcaaggac 
      tatctgagg cgttcgcgt tttgttttc gccaaagtg 
      caaaacact gacttgaag gacgtcgac ttgatttac 
      caacacgtc acaaaaact ggtatttat gttttactg 
      agacgtccc caagacttg cctgaaaat tacacggtt 
      gtgatggca aacaacaac aactacaat tgggtcaaa 
      gacttgaaa atggcagtg aaaaaatac cgttcctcc 
      caatcccgc atttattta atcaacgaa gacttcaat 
      tcgggaata gttggttgg tttaataca ataagacac 
      gaacagtac tgtcccaac attcgtttg ttcctcatc 
      aggagctac aaagaggtg gaaccgttc gccctcacc 
      tcaaaattc tactcccgg cagttgaag aaggacctc 
      acggtgaat gtgtaccag gacggggct tgggggacg 
      taccgccgg ttgcccctg gagccgttc cagccccgg 
      ctgtacgcc gatgcctgt cttatgaag gataacagt 
      ggggttaag tgggtggag aagtcgccg tatgtcacc 
      aagtgcgag attatggtg cattttgcg agtctgagt 
      agttgggat ttggcgaaa atcacaggg aagggcggg 
      gatgccgag tatgagttg ctggggttg gagtattcg 
      gggagttac tacgccctg tttgtgcaa acccacctc 
      tcccccaag gactcaatc ctcatccac aactccggc 
      actgacgtc agcatggcc gctatcgcc ctcgccacc 
      aacaaaaac tgcaccgtt tacatcgtc atcaactcc 
      tccaaccgg gacgtgtta cgcaaattc cccaaaatc 
      caggaaaac aacatcatc tacgacagc gaaggcagt 
      cccttctac gaaaccctc atgaccaaa acgaacggc 
      aaaggcgtc aacacaatc ttcaactgc aactcaatc 
      gaactcctc aaactctcc aaaaaatgc atcgccaaa 
      aacggccgc ttcatcgag ttcggcaac agcttaggc 
      acgttcttg gaaactgaa agtttgatg gaaaacaac 
      tgttccctg tacaacatc aatgtgaac gacgtcttc 
      gacgaaacc cccgagatc ctcgacatc atagccaag 
      gatattgat agatatgtc aaagagggc gtggttgtg 
      ccgctcaaa tactcggtg tatggcaag gatgacatc 
      gagatcgcg ttgaagaac ctggaggat aaggactac 
      gtgaagaag tgtttgatc aagattaag gacaacggt 
      gggttcttc tccaagccg gagaaccgc atcctggcg 
      ttcccgcgc acttacatg gaccccagc aagtgctac 
      atcgtgatc gggggcttg ggtgggttc ggtttcgag 
      ttgaccaag tggctgatt gaacgtggt gccagcaag 
      atcgtgatc aactcgcgc cgggaggtg cgctccggt 
      taccacgcc agttgcctg cagaagtgg aacaaccag 
      aagatcaca gtcttgacc aacacctcc gacactgcg 
      acgctcgaa ggcgccgaa tccttaatc aacttcgcc 
      acgaacttg gcacctgtc ggtggcatc ttcaacgcc 
      gcgctgcac gtgaaggac ggcttcatg accgacttg 
      gggcccgag gatttcgca gcggtggcc ggatccaag 
      attttcagt gcccagaac atggatatc ctgtcacgg 
      aaactgtgc aagaaactc gactatttc ctcatgttc 
      tcttccatt gcgtcgagt attggaaac gcggtacaa 
      tccaattac gcgttcgtc aactcggcg gttgagcgt 
      ctctgtgag acgcgcaag gctgagggg ctgccggcg 
      ctggcgttg gaatggggg ccgttggcc gatgttggt 
      attttggtg cgcttgaaa atgacgaac ccgattctg 
      gagactgtg aggcagaag ttgagttcg tgtctgagg 
      gttgttgac cagtatatg cagcaaagt gagccggtt 
      gtcaccagt tgtgcgtat ccagatcag tcgggggaa 
      gttagcgag aagaagacg ctggcggag ttggttgct 
      ggcttgttt gggatcaag aatttggag gagattgaa 
      gataatacg actttgttg gagttggga atggattcg 
      ttgcttggg tttgaagtc aagcatatt attaccaaa 
      gagtcgggc ttggatgtt agtgtgaac gatatacga 
      aatatgacc ttcgagaag attaaggaa atgtcggca 
      cctgcttaa gtggggtgg ggatggggt tggttattg 
      gttgtatgt tatatacaa ttactgatt attaaacaa 
      tctcatgca ca
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