| General information | |||
| TC007685 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 911474..913671 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007685 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01244 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
remarks: entire repetition development delayed, later than schedule, hours 60
- >TC007685 RA
MNPCSSHLQ KEDILLQIR DNLRKNNVK LWLDPYFSE TSGPSVNTL KNLAQNYSQ DINASVDNC YEALLELQK NSLENLKQN NHYKESGEA TLKIKILHP NSPPRMLTK ELLLSLRGS DVKEIIAKE INMSRDKLK LIHAGKVLS DSENLSSQG VKNGQQIMA ITFSEMSPE LKETENQIR ELEGVKTDS RLLALDNEY LQLEDQFGN IVKIPPEEK RALVVAMAL HEKGRSALK REDYSRALI FFLEADEEF KRCTSQILN SVDNYALLD LDIAWCYLC LQSVANLPE AFERLKRCE ERFHASYGP NLERLKAVK GVTGNEATL FMRLHLLQA IVLYHKNKR NEALKLLKK AQEELSALK VEDDSVTML VELGYSMAE ARLGLRATG GDVNKAANY IDENRNQRS QARQKALAE EILQRKRMK LGKCIDGKQ YVDPNFVKI LVNMGFNKE AARIALKHT NNVISDSIQ YIQEHPQPG PSQSKSIEF LALINDLVP ELEAAGFDP TMSKLALHK HGGDIMKAA EDLLANNGI IDGDWSILK ADSSSQVVD VAKKEKKDE AFKRIAGDI SMVDDDHLD LTLVQEELF LNQYLSLLA NDK
- >TC007685 RA
atgaacccg tgttcttct catctgcaa aaagaagac attcttttg caaatcagg gataatttg cgtaagaac aatgtgaaa ctttggcta gatccgtat tttagtgag acaagtggt ccctcagta aacacgtta aagaatttg gcccaaaat tacagtcaa gacattaac gcttctgtt gataattgt tacgaggca cttctcgaa ctccaaaaa aattcactg gagaattta aaacaaaac aaccattac aaagaatca ggcgaggca acgcttaaa atcaagatc ttgcatccg aatagcccc ccacgaatg ctcacgaaa gaattgtta ttgtcgtta cggggctcc gatgttaag gaaattatt gcgaaggaa ataaacatg agtcgtgat aaattgaag ttaattcac gccgggaaa gtattatcc gattctgag aatttgagc agtcaaggt gtgaaaaac ggccaacaa attatggca atcacattt tcggaaatg tctcccgag cttaaagaa accgaaaac cagatccgt gaactggag ggagtgaaa accgactcg cgcctttta gccctcgat aatgaatat cttcaattg gaggatcag tttggcaat attgtcaaa attcctcct gaggaaaaa cgggcctta gttgtggca atggccttg catgagaaa gggcgaagc gctttaaaa cgtgaagat tactcgcgt gctttgatt ttttttctt gaagccgac gaagaattc aaacgctgt acttcgcaa attttaaat agtgttgat aattacgct ctactagac ctcgatatt gcctggtgt tatttgtgt ttgcaaagt gttgcgaat ttaccggag gcctttgag aggcttaaa aggtgcgaa gagcggttt catgccagt tatgggccg aatttggag cggctcaag gctgtaaaa ggtgtcaca ggcaatgag gcgactttg tttatgcgc ctccacctg ctccaggcc atagttttg taccacaaa aacaagcga aatgaggcg ttaaaatta ctgaaaaag gcacaagag gagctttcg gccctcaaa gtcgaggat gatagtgtt acaatgttg gtagaactg ggctattct atggccgag gctcggttg gggttgcga gccactggc ggtgacgtt aataaagcg gccaattat atcgacgag aatagaaat caaaggtcg caagctaga cagaaagcc ttggctgag gaaattttg caaagaaaa agaatgaag ttggggaaa tgtattgac ggtaaacaa tacgtggac cccaacttt gttaaaatt ctagtcaat atggggttt aacaaggag gcggcgcgc attgccttg aaacacacc aataatgtc atttcggat agtattcag tatattcaa gagcaccca cagcctggt cccagtcag agcaagagt attgaattt ttggcattg attaatgat ctagtgccg gagttggaa gcggcaggg tttgatccg accatgtcc aagttagcg ttgcacaaa catggtggt gatattatg aaagcagct gaggattta ttggccaat aacgggatt atcgatggg gattggtcc attttgaaa gctgattcg agctcgcaa gtagttgat gtggcaaaa aaagaaaaa aaggacgag gcgttcaag cgcatagct ggtgatatt tccatggtg gatgatgac catttagat ctgactttg gttcaggaa gaactgttc ctcaatcaa tacttgtcg ctcctcgcc aatgataaa taa
- >TC007685 RA
tgacatgat gtaaacaaa cactcgtgt gtcaaattt gatgacttg aaaattgca aaaatgaac ccgtgttct tctcatctg caaaaagaa gacattctt ttgcaaatc agggataat ttgcgtaag aacaatgtg aaactttgg ctagatccg tattttagt gagacaagt ggtccctca gtaaacacg ttaaagaat ttggcccaa aattacagt caagacatt aacgcttct gttgataat tgttacgag gcacttctc gaactccaa aaaaattca ctggagaat ttaaaacaa aacaaccat tacaaagaa tcaggcgag gcaacgctt aaaatcaag atcttgcat ccgaatagc cccccacga atgctcacg aaagaattg ttattgtcg ttacggggc tccgatgtt aaggaaatt attgcgaag gaaataaac atgagtcgt gataaattg aagttaatt cacgccggg aaagtatta tccgattct gagaatttg agcagtcaa ggtgtgaaa aacggccaa caaattatg gcaatcaca ttttcggaa atgtctccc gagcttaaa gaaaccgaa aaccagatc cgtgaactg gagggagtg aaaaccgac tcgcgcctt ttagccctc gataatgaa tatcttcaa ttggaggat cagtttggc aatattgtc aaaattcct cctgaggaa aaacgggcc ttagttgtg gcaatggcc ttgcatgag aaagggcga agcgcttta aaacgtgaa gattactcg cgtgctttg atttttttt cttgaagcc gacgaagaa ttcaaacgc tgtacttcg caaatttta aatagtgtt gataattac gctctacta gacctcgat attgcctgg tgttatttg tgtttgcaa agtgttgcg aatttaccg gaggccttt gagaggctt aaaaggtgc gaagagcgg tttcatgcc agttatggg ccgaatttg gagcggctc aaggctgta aaaggtgtc acaggcaat gaggcgact ttgtttatg cgcctccac ctgctccag gccatagtt ttgtaccac aaaaacaag cgaaatgag gcgttaaaa ttactgaaa aaggcacaa gaggagctt tcggccctc aaagtcgag gatgatagt gttacaatg ttggtagaa ctgggctat tctatggcc gaggctcgg ttggggttg cgagccact ggcggtgac gttaataaa gcggccaat tatatcgac gagaataga aatcaaagg tcgcaagct agacagaaa gccttggct gaggaaatt ttgcaaaga aaaagaatg aagttgggg aaatgtatt gacggtaaa caatacgtg gaccccaac tttgttaaa attctagtc aatatgggg tttaacaag gaggcggcg cgcattgcc ttgaaacac accaataat gtcatttcg gatagtatt cagtatatt caagagcac ccacagcct ggtcccagt cagagcaag agtattgaa tttttggca ttgattaat gatctagtg ccggagttg gaagcggca gggtttgat ccgaccatg tccaagtta gcgttgcac aaacatggt ggtgatatt atgaaagca gctgaggat ttattggcc aataacggg attatcgat ggggattgg tccattttg aaagctgat tcgagctcg caagtagtt gatgtggca aaaaaagaa aaaaaggac gaggcgttc aagcgcata gctggtgat atttccatg gtggatgat gaccattta gatctgact ttggttcag gaagaactg ttcctcaat caatacttg tcgctcctc gccaatgat aaataaacc cgttacgca ttgtctatt tttatagaa tttttattc gtctttaat caaatacaa taaatcaag acccgaatc
