Status
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Gene information
General information
TC007685 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 911474..913671
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007685
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01244

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    remarks: entire repetition development delayed, later than schedule, hours 60
    Pupal morphology
    remarks: entire repetition development delayed, later than schedule, hours 60
    Adult morphology
    remarks: entire repetition development delayed, later than schedule, hours 60
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007685 RA
      MNPCSSHLQ KEDILLQIR DNLRKNNVK LWLDPYFSE 
      TSGPSVNTL KNLAQNYSQ DINASVDNC YEALLELQK 
      NSLENLKQN NHYKESGEA TLKIKILHP NSPPRMLTK 
      ELLLSLRGS DVKEIIAKE INMSRDKLK LIHAGKVLS 
      DSENLSSQG VKNGQQIMA ITFSEMSPE LKETENQIR 
      ELEGVKTDS RLLALDNEY LQLEDQFGN IVKIPPEEK 
      RALVVAMAL HEKGRSALK REDYSRALI FFLEADEEF 
      KRCTSQILN SVDNYALLD LDIAWCYLC LQSVANLPE 
      AFERLKRCE ERFHASYGP NLERLKAVK GVTGNEATL 
      FMRLHLLQA IVLYHKNKR NEALKLLKK AQEELSALK 
      VEDDSVTML VELGYSMAE ARLGLRATG GDVNKAANY 
      IDENRNQRS QARQKALAE EILQRKRMK LGKCIDGKQ 
      YVDPNFVKI LVNMGFNKE AARIALKHT NNVISDSIQ 
      YIQEHPQPG PSQSKSIEF LALINDLVP ELEAAGFDP 
      TMSKLALHK HGGDIMKAA EDLLANNGI IDGDWSILK 
      ADSSSQVVD VAKKEKKDE AFKRIAGDI SMVDDDHLD 
      LTLVQEELF LNQYLSLLA NDK
    CDS sequence
    • >TC007685 RA
      atgaacccg tgttcttct catctgcaa aaagaagac 
      attcttttg caaatcagg gataatttg cgtaagaac 
      aatgtgaaa ctttggcta gatccgtat tttagtgag 
      acaagtggt ccctcagta aacacgtta aagaatttg 
      gcccaaaat tacagtcaa gacattaac gcttctgtt 
      gataattgt tacgaggca cttctcgaa ctccaaaaa 
      aattcactg gagaattta aaacaaaac aaccattac 
      aaagaatca ggcgaggca acgcttaaa atcaagatc 
      ttgcatccg aatagcccc ccacgaatg ctcacgaaa 
      gaattgtta ttgtcgtta cggggctcc gatgttaag 
      gaaattatt gcgaaggaa ataaacatg agtcgtgat 
      aaattgaag ttaattcac gccgggaaa gtattatcc 
      gattctgag aatttgagc agtcaaggt gtgaaaaac 
      ggccaacaa attatggca atcacattt tcggaaatg 
      tctcccgag cttaaagaa accgaaaac cagatccgt 
      gaactggag ggagtgaaa accgactcg cgcctttta 
      gccctcgat aatgaatat cttcaattg gaggatcag 
      tttggcaat attgtcaaa attcctcct gaggaaaaa 
      cgggcctta gttgtggca atggccttg catgagaaa 
      gggcgaagc gctttaaaa cgtgaagat tactcgcgt 
      gctttgatt ttttttctt gaagccgac gaagaattc 
      aaacgctgt acttcgcaa attttaaat agtgttgat 
      aattacgct ctactagac ctcgatatt gcctggtgt 
      tatttgtgt ttgcaaagt gttgcgaat ttaccggag 
      gcctttgag aggcttaaa aggtgcgaa gagcggttt 
      catgccagt tatgggccg aatttggag cggctcaag 
      gctgtaaaa ggtgtcaca ggcaatgag gcgactttg 
      tttatgcgc ctccacctg ctccaggcc atagttttg 
      taccacaaa aacaagcga aatgaggcg ttaaaatta 
      ctgaaaaag gcacaagag gagctttcg gccctcaaa 
      gtcgaggat gatagtgtt acaatgttg gtagaactg 
      ggctattct atggccgag gctcggttg gggttgcga 
      gccactggc ggtgacgtt aataaagcg gccaattat 
      atcgacgag aatagaaat caaaggtcg caagctaga 
      cagaaagcc ttggctgag gaaattttg caaagaaaa 
      agaatgaag ttggggaaa tgtattgac ggtaaacaa 
      tacgtggac cccaacttt gttaaaatt ctagtcaat 
      atggggttt aacaaggag gcggcgcgc attgccttg 
      aaacacacc aataatgtc atttcggat agtattcag 
      tatattcaa gagcaccca cagcctggt cccagtcag 
      agcaagagt attgaattt ttggcattg attaatgat 
      ctagtgccg gagttggaa gcggcaggg tttgatccg 
      accatgtcc aagttagcg ttgcacaaa catggtggt 
      gatattatg aaagcagct gaggattta ttggccaat 
      aacgggatt atcgatggg gattggtcc attttgaaa 
      gctgattcg agctcgcaa gtagttgat gtggcaaaa 
      aaagaaaaa aaggacgag gcgttcaag cgcatagct 
      ggtgatatt tccatggtg gatgatgac catttagat 
      ctgactttg gttcaggaa gaactgttc ctcaatcaa 
      tacttgtcg ctcctcgcc aatgataaa taa
    mRNA sequence
    • >TC007685 RA
      tgacatgat gtaaacaaa cactcgtgt gtcaaattt 
      gatgacttg aaaattgca aaaatgaac ccgtgttct 
      tctcatctg caaaaagaa gacattctt ttgcaaatc 
      agggataat ttgcgtaag aacaatgtg aaactttgg 
      ctagatccg tattttagt gagacaagt ggtccctca 
      gtaaacacg ttaaagaat ttggcccaa aattacagt 
      caagacatt aacgcttct gttgataat tgttacgag 
      gcacttctc gaactccaa aaaaattca ctggagaat 
      ttaaaacaa aacaaccat tacaaagaa tcaggcgag 
      gcaacgctt aaaatcaag atcttgcat ccgaatagc 
      cccccacga atgctcacg aaagaattg ttattgtcg 
      ttacggggc tccgatgtt aaggaaatt attgcgaag 
      gaaataaac atgagtcgt gataaattg aagttaatt 
      cacgccggg aaagtatta tccgattct gagaatttg 
      agcagtcaa ggtgtgaaa aacggccaa caaattatg 
      gcaatcaca ttttcggaa atgtctccc gagcttaaa 
      gaaaccgaa aaccagatc cgtgaactg gagggagtg 
      aaaaccgac tcgcgcctt ttagccctc gataatgaa 
      tatcttcaa ttggaggat cagtttggc aatattgtc 
      aaaattcct cctgaggaa aaacgggcc ttagttgtg 
      gcaatggcc ttgcatgag aaagggcga agcgcttta 
      aaacgtgaa gattactcg cgtgctttg atttttttt 
      cttgaagcc gacgaagaa ttcaaacgc tgtacttcg 
      caaatttta aatagtgtt gataattac gctctacta 
      gacctcgat attgcctgg tgttatttg tgtttgcaa 
      agtgttgcg aatttaccg gaggccttt gagaggctt 
      aaaaggtgc gaagagcgg tttcatgcc agttatggg 
      ccgaatttg gagcggctc aaggctgta aaaggtgtc 
      acaggcaat gaggcgact ttgtttatg cgcctccac 
      ctgctccag gccatagtt ttgtaccac aaaaacaag 
      cgaaatgag gcgttaaaa ttactgaaa aaggcacaa 
      gaggagctt tcggccctc aaagtcgag gatgatagt 
      gttacaatg ttggtagaa ctgggctat tctatggcc 
      gaggctcgg ttggggttg cgagccact ggcggtgac 
      gttaataaa gcggccaat tatatcgac gagaataga 
      aatcaaagg tcgcaagct agacagaaa gccttggct 
      gaggaaatt ttgcaaaga aaaagaatg aagttgggg 
      aaatgtatt gacggtaaa caatacgtg gaccccaac 
      tttgttaaa attctagtc aatatgggg tttaacaag 
      gaggcggcg cgcattgcc ttgaaacac accaataat 
      gtcatttcg gatagtatt cagtatatt caagagcac 
      ccacagcct ggtcccagt cagagcaag agtattgaa 
      tttttggca ttgattaat gatctagtg ccggagttg 
      gaagcggca gggtttgat ccgaccatg tccaagtta 
      gcgttgcac aaacatggt ggtgatatt atgaaagca 
      gctgaggat ttattggcc aataacggg attatcgat 
      ggggattgg tccattttg aaagctgat tcgagctcg 
      caagtagtt gatgtggca aaaaaagaa aaaaaggac 
      gaggcgttc aagcgcata gctggtgat atttccatg 
      gtggatgat gaccattta gatctgact ttggttcag 
      gaagaactg ttcctcaat caatacttg tcgctcctc 
      gccaatgat aaataaacc cgttacgca ttgtctatt 
      tttatagaa tttttattc gtctttaat caaatacaa 
      taaatcaag acccgaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY