Status
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Gene information
General information
TC007685 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 911474..913671
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007685
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01244

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    remarks: entire repetition development delayed, later than schedule, hours 60
    Pupal morphology
    remarks: entire repetition development delayed, later than schedule, hours 60
    Adult morphology
    remarks: entire repetition development delayed, later than schedule, hours 60
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007685 RA
      MNPCSSHLQ KEDILLQIR DNLRKNNVK LWLDPYFSE 
TSGPSVNTL KNLAQNYSQ DINASVDNC YEALLELQK 
NSLENLKQN NHYKESGEA TLKIKILHP NSPPRMLTK 
ELLLSLRGS DVKEIIAKE INMSRDKLK LIHAGKVLS 
DSENLSSQG VKNGQQIMA ITFSEMSPE LKETENQIR 
ELEGVKTDS RLLALDNEY LQLEDQFGN IVKIPPEEK 
RALVVAMAL HEKGRSALK REDYSRALI FFLEADEEF 
KRCTSQILN SVDNYALLD LDIAWCYLC LQSVANLPE 
AFERLKRCE ERFHASYGP NLERLKAVK GVTGNEATL 
FMRLHLLQA IVLYHKNKR NEALKLLKK AQEELSALK 
VEDDSVTML VELGYSMAE ARLGLRATG GDVNKAANY 
IDENRNQRS QARQKALAE EILQRKRMK LGKCIDGKQ 
YVDPNFVKI LVNMGFNKE AARIALKHT NNVISDSIQ 
YIQEHPQPG PSQSKSIEF LALINDLVP ELEAAGFDP 
TMSKLALHK HGGDIMKAA EDLLANNGI IDGDWSILK 
ADSSSQVVD VAKKEKKDE AFKRIAGDI SMVDDDHLD 
LTLVQEELF LNQYLSLLA NDK
    CDS sequence
    • >TC007685 RA
      atgaacccg tgttcttct catctgcaa aaagaagac 
attcttttg caaatcagg gataatttg cgtaagaac 
aatgtgaaa ctttggcta gatccgtat tttagtgag 
acaagtggt ccctcagta aacacgtta aagaatttg 
gcccaaaat tacagtcaa gacattaac gcttctgtt 
gataattgt tacgaggca cttctcgaa ctccaaaaa 
aattcactg gagaattta aaacaaaac aaccattac 
aaagaatca ggcgaggca acgcttaaa atcaagatc 
ttgcatccg aatagcccc ccacgaatg ctcacgaaa 
gaattgtta ttgtcgtta cggggctcc gatgttaag 
gaaattatt gcgaaggaa ataaacatg agtcgtgat 
aaattgaag ttaattcac gccgggaaa gtattatcc 
gattctgag aatttgagc agtcaaggt gtgaaaaac 
ggccaacaa attatggca atcacattt tcggaaatg 
tctcccgag cttaaagaa accgaaaac cagatccgt 
gaactggag ggagtgaaa accgactcg cgcctttta 
gccctcgat aatgaatat cttcaattg gaggatcag 
tttggcaat attgtcaaa attcctcct gaggaaaaa 
cgggcctta gttgtggca atggccttg catgagaaa 
gggcgaagc gctttaaaa cgtgaagat tactcgcgt 
gctttgatt ttttttctt gaagccgac gaagaattc 
aaacgctgt acttcgcaa attttaaat agtgttgat 
aattacgct ctactagac ctcgatatt gcctggtgt 
tatttgtgt ttgcaaagt gttgcgaat ttaccggag 
gcctttgag aggcttaaa aggtgcgaa gagcggttt 
catgccagt tatgggccg aatttggag cggctcaag 
gctgtaaaa ggtgtcaca ggcaatgag gcgactttg 
tttatgcgc ctccacctg ctccaggcc atagttttg 
taccacaaa aacaagcga aatgaggcg ttaaaatta 
ctgaaaaag gcacaagag gagctttcg gccctcaaa 
gtcgaggat gatagtgtt acaatgttg gtagaactg 
ggctattct atggccgag gctcggttg gggttgcga 
gccactggc ggtgacgtt aataaagcg gccaattat 
atcgacgag aatagaaat caaaggtcg caagctaga 
cagaaagcc ttggctgag gaaattttg caaagaaaa 
agaatgaag ttggggaaa tgtattgac ggtaaacaa 
tacgtggac cccaacttt gttaaaatt ctagtcaat 
atggggttt aacaaggag gcggcgcgc attgccttg 
aaacacacc aataatgtc atttcggat agtattcag 
tatattcaa gagcaccca cagcctggt cccagtcag 
agcaagagt attgaattt ttggcattg attaatgat 
ctagtgccg gagttggaa gcggcaggg tttgatccg 
accatgtcc aagttagcg ttgcacaaa catggtggt 
gatattatg aaagcagct gaggattta ttggccaat 
aacgggatt atcgatggg gattggtcc attttgaaa 
gctgattcg agctcgcaa gtagttgat gtggcaaaa 
aaagaaaaa aaggacgag gcgttcaag cgcatagct 
ggtgatatt tccatggtg gatgatgac catttagat 
ctgactttg gttcaggaa gaactgttc ctcaatcaa 
tacttgtcg ctcctcgcc aatgataaa taa
    mRNA sequence
    • >TC007685 RA
      tgacatgat gtaaacaaa cactcgtgt gtcaaattt 
gatgacttg aaaattgca aaaatgaac ccgtgttct 
tctcatctg caaaaagaa gacattctt ttgcaaatc 
agggataat ttgcgtaag aacaatgtg aaactttgg 
ctagatccg tattttagt gagacaagt ggtccctca 
gtaaacacg ttaaagaat ttggcccaa aattacagt 
caagacatt aacgcttct gttgataat tgttacgag 
gcacttctc gaactccaa aaaaattca ctggagaat 
ttaaaacaa aacaaccat tacaaagaa tcaggcgag 
gcaacgctt aaaatcaag atcttgcat ccgaatagc 
cccccacga atgctcacg aaagaattg ttattgtcg 
ttacggggc tccgatgtt aaggaaatt attgcgaag 
gaaataaac atgagtcgt gataaattg aagttaatt 
cacgccggg aaagtatta tccgattct gagaatttg 
agcagtcaa ggtgtgaaa aacggccaa caaattatg 
gcaatcaca ttttcggaa atgtctccc gagcttaaa 
gaaaccgaa aaccagatc cgtgaactg gagggagtg 
aaaaccgac tcgcgcctt ttagccctc gataatgaa 
tatcttcaa ttggaggat cagtttggc aatattgtc 
aaaattcct cctgaggaa aaacgggcc ttagttgtg 
gcaatggcc ttgcatgag aaagggcga agcgcttta 
aaacgtgaa gattactcg cgtgctttg atttttttt 
cttgaagcc gacgaagaa ttcaaacgc tgtacttcg 
caaatttta aatagtgtt gataattac gctctacta 
gacctcgat attgcctgg tgttatttg tgtttgcaa 
agtgttgcg aatttaccg gaggccttt gagaggctt 
aaaaggtgc gaagagcgg tttcatgcc agttatggg 
ccgaatttg gagcggctc aaggctgta aaaggtgtc 
acaggcaat gaggcgact ttgtttatg cgcctccac 
ctgctccag gccatagtt ttgtaccac aaaaacaag 
cgaaatgag gcgttaaaa ttactgaaa aaggcacaa 
gaggagctt tcggccctc aaagtcgag gatgatagt 
gttacaatg ttggtagaa ctgggctat tctatggcc 
gaggctcgg ttggggttg cgagccact ggcggtgac 
gttaataaa gcggccaat tatatcgac gagaataga 
aatcaaagg tcgcaagct agacagaaa gccttggct 
gaggaaatt ttgcaaaga aaaagaatg aagttgggg 
aaatgtatt gacggtaaa caatacgtg gaccccaac 
tttgttaaa attctagtc aatatgggg tttaacaag 
gaggcggcg cgcattgcc ttgaaacac accaataat 
gtcatttcg gatagtatt cagtatatt caagagcac 
ccacagcct ggtcccagt cagagcaag agtattgaa 
tttttggca ttgattaat gatctagtg ccggagttg 
gaagcggca gggtttgat ccgaccatg tccaagtta 
gcgttgcac aaacatggt ggtgatatt atgaaagca 
gctgaggat ttattggcc aataacggg attatcgat 
ggggattgg tccattttg aaagctgat tcgagctcg 
caagtagtt gatgtggca aaaaaagaa aaaaaggac 
gaggcgttc aagcgcata gctggtgat atttccatg 
gtggatgat gaccattta gatctgact ttggttcag 
gaagaactg ttcctcaat caatacttg tcgctcctc 
gccaatgat aaataaacc cgttacgca ttgtctatt 
tttatagaa tttttattc gtctttaat caaatacaa 
taaatcaag acccgaatc

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY