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Gene information
General information
TC007683 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 924576..925871
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007683
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01243

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007683 RA
      MAGVVRRIF ANLPRINTC NYVKTPLLS 
      ILPSVTSKF YSNVPTPYE IVDAQEDRK 
      TYQGVIDNV NRQVAEKKQ GRLFAVVHV 
      AGKQVKITE GDVFIVEGY WPPDAGDKI 
      SLDKVLLVG ASDFTLIGR PLVQRGLVR 
      VEATVIEKN VSHTKTHFR KKRRKQYMR 
      INFYRTPRT MVRVNRIEI VGEVNHPPA 
      VTGLETRIF
    CDS sequence
    • >TC007683 RA
      atggcgggg gtagtgcgt cgaattttt gcaaatctc 
      ccccgaatt aacacgtgt aactacgta aaaaccccc 
      cttttgtct attctacca agcgttact tcgaaattt 
      tactcaaac gtgcccacg ccgtatgaa atagtggac 
      gcacaagaa gaccgcaaa acctaccaa ggcgttatt 
      gataatgtt aaccgacaa gtggcggaa aaaaaacag 
      gggcgcctg tttgcggtg gtccacgtg gccgggaaa 
      caagtcaaa ataacagaa ggggatgtc ttcatagtg 
      gagggttat tggccccca gatgctggc gataaaatt 
      agccttgac aaggtttta ctagttggg gctagtgat 
      tttacgctg attgggcgc cctcttgtc cagaggggc 
      cttgtgagg gtggaggct actgtcatt gagaagaat 
      gtctcgcac accaaaact cacttcagg aagaagagg 
      aggaaacag tacatgagg attaatttt tataggacg 
      cctaggacc atggttaga gtcaatagg attgaaata 
      gtgggtgag gtgaatcac ccgcctgct gtgacgggc 
      ttagagacg cgcattttt taa
    mRNA sequence
    • >TC007683 RA
      aaagtgtgg cacctacct aaataagca ccgtccaag 
      tgaatatag ccatgacgg aacgtgaaa aacctcaac 
      ataactctc caaacactt taaacactt ctcatttct 
      atttttcaa cttccaaaa cactgaacg cctgttgat 
      gttgtcacc tgtcaattg tcaaaaact taacctcgg 
      aaaatggcg ggggtagtg cgtcgaatt tttgcaaat 
      ctcccccga attaacacg tgtaactac gtaaaaacc 
      ccccttttg tctattcta ccaagcgtt acttcgaaa 
      ttttactca aacgtgccc acgccgtat gaaatagtg 
      gacgcacaa gaagaccgc aaaacctac caaggcgtt 
      attgataat gttaaccga caagtggcg gaaaaaaaa 
      caggggcgc ctgtttgcg gtggtccac gtggccggg 
      aaacaagtc aaaataaca gaaggggat gtcttcata 
      gtggagggt tattggccc ccagatgct ggcgataaa 
      attagcctt gacaaggtt ttactagtt ggggctagt 
      gattttacg ctgattggg cgccctctt gtccagagg 
      ggccttgtg agggtggag gctactgtc attgagaag 
      aatgtctcg cacaccaaa actcacttc aggaagaag 
      aggaggaaa cagtacatg aggattaat ttttatagg 
      acgcctagg accatggtt agagtcaat aggattgaa 
      atagtgggt gaggtgaat cacccgcct gctgtgacg 
      ggcttagag acgcgcatt ttttaaatt aatatgatt 
      tatttacta aaatacaca gcattacaa actgaacac 
      agttatatc atggatata gtcggtagt aggtaaaaa 
      attgtataa aaaaattga tacaaatcc tagtcgtac 
      aggcgtttc gtgtttttc ttttggaat aaaaagtaa 
      cggaaatga ttgtttaat tgtctagcc caatctatc 
      acaattacc atagatttc tttaagtaa ctaacagtc 
      aaatgccaa ttcattgtg cgtcgagta acgcgtttt 
      acacaataa agtcttata ataaatgca acaatactt 
      tgattaaat acataaact tggtattcc tacagatca 
      caaacgact ggcttatca aggttatat acgctctct 
      atcgtcact aaactaaaa cgaatatca caaaaccta 
      acataatcc cgccgactc tttaaagca cgattaaaa 
      ggactaaat aaataacca gcttttcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY