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Gene information
General information
TC007680 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 939985..941211
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007680
    iBeetle screen iB_01242

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007680 RA
      MSESKRDNH AKDGIIDFT AGFLGGIAL VYVGQPLDT 
      VKVKMQTFP SLYNNMIDC FMKTLRADG IYRGLYAGT 
      VPALAANIT ENSVLFLCY GFCQKFIQK VSYTEHVAD 
      LSMMQNATA GFLASFFSS VAICPTELL KCKLQAMFE 
      TQKQQEMQG FKVVRVGPM KLAAQILKT EGPLGFFHG 
      LVPTLVREM PGYFFFFGT YEGTRTLLA RPGESKDDI 
      GLFKTMVAG ALGGMSFWT LTYPVDVAK SRIQVTNSK 
      MNMVAMILQ ISRREGFRQ LYNGLTPTL VRTIPSTAT 
      LFATYEYSK RFLHQIF
    CDS sequence
    • >TC007680 RA
      atgtccgaa tctaagagg gacaaccat gctaaagac 
      ggaatcatt gatttcacg gccggtttt ttggggggc 
      atcgcgttg gtttacgtc ggccagccc ctggacacc 
      gtcaaggta aaaatgcag acgtttcca agcttatac 
      aacaacatg attgattgt ttcatgaaa acgttacgc 
      gcagatgga atttatcgc ggtttgtat gccggtaca 
      gtcccggct ttggcggca aatattacc gaaaattcc 
      gttttgttt ctttgttac ggcttttgc cagaaattt 
      attcaaaaa gttagttat accgaacac gtggctgat 
      ctaagcatg atgcaaaat gccacagcg ggttttttg 
      gcctcgttt ttctcttcg gtggcgatt tgccccacg 
      gaacttctc aaatgtaag ctccaagcg atgtttgag 
      acgcaaaaa cagcaagaa atgcaaggg ttcaaagtg 
      gtcagagtg gggcccatg aagctggca gcgcaaata 
      ttgaagacc gaagggcct ttggggttt tttcacggg 
      cttgtgccc acgttagtt agggaaatg ccggggtat 
      tttttcttt tttgggact tatgaaggg actaggact 
      ttgctagcg cgtccgggg gagtccaag gacgatata 
      ggcttgttt aagacaatg gtggcgggg gctttgggg 
      ggcatgtct ttctggacg ttgacttat ccagtcgat 
      gtggccaaa agtcggatt caagtcact aatagtaaa 
      atgaatatg gttgcaatg attttgcaa atatcgagg 
      cgggaaggc ttcagacag ctttataac gggttgact 
      ccaacgtta gttcggact attccatcc actgccact 
      ctttttgca acttacgag tattccaaa agattcctc 
      caccaaatt ttctga
    mRNA sequence
    • >TC007680 RA
      cggataagt taattgtta tcagtactt cacatgcca 
      tgctgaaga aatgtccga atctaagag ggacaacca 
      tgctaaaga cggaatcat tgatttcac ggccggttt 
      tttgggggg catcgcgtt ggtttacgt cggccagcc 
      cctggacac cgtcaaggt aaaaatgca gacgtttcc 
      aagcttata caacaacat gattgattg tttcatgaa 
      aacgttacg cgcagatgg aatttatcg cggtttgta 
      tgccggtac agtcccggc tttggcggc aaatattac 
      cgaaaattc cgttttgtt tctttgtta cggcttttg 
      ccagaaatt tattcaaaa agttagtta taccgaaca 
      cgtggctga tctaagcat gatgcaaaa tgccacagc 
      gggtttttt ggcctcgtt tttctcttc ggtggcgat 
      ttgccccac ggaacttct caaatgtaa gctccaagc 
      gatgtttga gacgcaaaa acagcaaga aatgcaagg 
      gttcaaagt ggtcagagt ggggcccat gaagctggc 
      agcgcaaat attgaagac cgaagggcc tttggggtt 
      ttttcacgg gcttgtgcc cacgttagt tagggaaat 
      gccggggta ttttttctt ttttgggac ttatgaagg 
      gactaggac tttgctagc gcgtccggg ggagtccaa 
      ggacgatat aggcttgtt taagacaat ggtggcggg 
      ggctttggg gggcatgtc tttctggac gttgactta 
      tccagtcga tgtggccaa aagtcggat tcaagtcac 
      taatagtaa aatgaatat ggttgcaat gattttgca 
      aatatcgag gcgggaagg cttcagaca gctttataa 
      cgggttgac tccaacgtt agttcggac tattccatc 
      cactgccac tctttttgc aacttacga gtattccaa 
      aagattcct ccaccaaat tttctgatt agaaataaa 
      actcgaaca tagttttta tattttatt tttatccaa 
      taaataaat aaataggaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY