Status
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Gene information
General information
TC007679 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 970017..973953
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007679
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01241

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007679 RA
      MSIVCEEVF DETSHPSAE EIRDYATKI GIDPDSEPQ 
      LLPLAAEGL MKALPPGWR PCYDDKSKS YYYYNNNTG 
      KTQWEHPLD DVYRGLVKK VRAESQSLS LGEPTEDAT 
      YARDEIPSF EEPPKLTKL KLSPLKTSP KHEAKLLKQ 
      RSEEHLLSS RKISLNIFN SFDEKSNFE KHPRVLDKP 
      ELKISGGGS MFLKCNTKK GADLAPSPA KSDSPDVQN 
      QPRSILRER NSLEVSRSM DFDKVSLDK SEKDDDDKK 
      SVRFNLDTN TDVGFTFSD KSTSSDDDI GNDIINVKV 
      TPNKPKSRF TVSPVPDLT NEKKLIKPN PTDFIKPKL 
      TISKGSDSE DDSLKSNGS DESPLHTKM LIKSKKLEE 
      KAEKKIAQM KQNIWEEKN DELVKFRDD LQESQKEEL 
      ERILINAKS DHEDRIKSE LENLRIEME NRNKGALNE 
      ERIKFEKLL EETRGKFEK DFEQEQSQM KEKFEAKKE 
      ELEKYYEEK LVEIEKDLA ERMEKNRDE MILTHNSNV 
      EQLKQNHAI IIEELKKGF KAEEDALRR DHKAVIEEL 
      KTSKVDDRK DDKFEKIRC EKRLLEDKY RCLKEKYLR 
      LKTDVKMSI EKRNKRKEQ SGATTTTGS ETERSHSNN 
      KDRTPLNSP IRKPPRSET KKPEHRATR QLIIQDLDT 
      SISDKSENK KDCDSSDST NPGRSRKKI FSRLKSSST 
      SRINTGSKS RRNQRSCSP VENLRRQLQ KLEDLEDQF 
      PQNPQADTY HLRIHLERD SIKRAKESL RNQKSLFQQ 
      RQKELKLKH GSMARHTLQ QLCQEEKEL TDMEVSLHR 
      TRSLLGEKV IRLRHLEQS LQRATMTAE NRQEDATLS 
      DISSHSASS GISSTEFAT ADPNPGKSF IHGEHLQES 
      SEIIQSLEN LNSEIREIW DVLRKQQQQ GGISVIPPL 
      VYPDLGWPL LAGAATVPA PPSIPTLAD RLHNYRQHV 
      ALANAQSTV VTHANQGAT TTLVERTRN LRHWLRQAG 
      VDGNMEGST PQATL
    CDS sequence
    • >TC007679 RA
      atgtccatt gtttgcgaa gaagtgttc gatgagacg 
      agccaccct tctgctgaa gaaatccgg gactatgcc 
      acaaaaatc gggatcgac ccggactcc gagccccag 
      ctgctcccg cttgcagcc gagggcctc atgaaggct 
      ttgcccccc ggttggaga ccttgctat gacgacaaa 
      tcaaaatca tactactac tacaacaac aacacggga 
      aaaacacaa tgggagcac cctctcgac gacgtttac 
      cgcggtttg gtgaaaaaa gtccgtgcc gaaagccaa 
      tctctaagt cttggtgaa cccaccgaa gatgcaact 
      tatgccaga gatgaaatt ccaagtttt gaggagcct 
      ccaaaactc accaaactc aaactttcg ccactcaaa 
      accagcccc aagcacgaa gccaaactc ctcaaacaa 
      cgctcggag gagcattta ctttcgtcg cggaaaatt 
      agtctcaat attttcaat agtttcgat gagaaaagc 
      aattttgag aaacatcca cgtgtgttg gataaacca 
      gaattgaag ataagtggt ggaggatca atgtttctg 
      aagtgtaac acaaagaaa ggcgctgat ttggcccca 
      agtccggca aagagtgat tccccggat gtgcaaaac 
      caaccaagg agtatcctc agggaacgc aattccttg 
      gaagtctcc cggagtatg gattttgat aaggtttct 
      ttagacaag tcggagaag gacgatgat gataagaaa 
      agtgtgagg tttaacctc gatacgaac accgatgtt 
      ggctttaca ttttcggat aaatcaact tccagtgat 
      gatgacata gggaatgac atcatcaat gttaaagtg 
      actccaaat aagccaaaa tcacgattt accgtaagc 
      ccggtcccc gacttaacg aacgaaaag aaactaatt 
      aagccaaac ccaaccgac tttattaaa ccaaaatta 
      acaataagt aaaggttcc gactccgaa gatgactct 
      cttaaaagt aacggttcg gacgaaagt cctctccac 
      accaaaatg ttgatcaaa tcgaagaaa ctagaggaa 
      aaagccgag aaaaaaatt gcccaaatg aaacaaaac 
      atctgggag gagaaaaac gatgagttg gtgaaattc 
      cgtgacgac ttacaagag tcacaaaag gaggaactt 
      gaacgcatt ttaattaat gctaaaagt gaccacgaa 
      gatcggatc aaatcagag ttggagaat ttgcgaatt 
      gagatggaa aatcgcaac aaaggggcg ctaaacgag 
      gaaaggatc aagtttgag aagttattg gaagagacg 
      aggggaaag tttgaaaaa gattttgaa caagaacaa 
      tcgcaaatg aaggaaaag ttcgaggca aagaaagaa 
      gagttggag aagtactac gaggagaaa ttggtcgaa 
      atcgagaaa gacttggcc gaaagaatg gagaaaaat 
      cgtgacgag atgattcta acgcacaac tcaaacgtc 
      gaacaattg aaacagaac catgctata attatcgag 
      gagttgaaa aaagggttc aaggctgag gaagatgcc 
      ttgagacgg gaccacaag gctgtgatt gaggaacta 
      aagactagt aaagtagac gataggaaa gacgataag 
      tttgaaaaa atccgttgt gagaaacga ctgctagaa 
      gataagtac cgatgcttg aaggagaaa tatttgaga 
      ctgaaaacc gacgtgaaa atgtcaatc gagaagcgg 
      aacaaaagg aaggaacaa agcggggcg acaacaacc 
      acagggtcg gaaacggaa cgcagtcac tccaacaac 
      aaagacagg actccgctg aattccccc atacgtaaa 
      cccccccgt tctgagacg aaaaaaccc gagcaccga 
      gcaacccgc caactaatc atccaagac ctggacact 
      tcaatcagc gataaaagc gaaaataaa aaagattgt 
      gattcgagt gactccaca aatccaggt cgcagccga 
      aaaaaaatt ttctcccgc ttgaaaagc tcctcaact 
      tcgaggatc aacacggga tcgaaatca cgtcgtaac 
      caacgttcg tgctcccct gttgagaat ttgcggcga 
      cagttgcag aaattggag gatttggag gaccagttc 
      cctcaaaat ccacaagct gatacgtat catttgagg 
      atacacttg gagcgggat tcgattaaa agggccaaa 
      gagagtttg aggaatcag aaatcgctc ttccagcag 
      agacagaaa gaattgaag ttgaaacac gggtctatg 
      gccaggcac acgttgcaa cagttgtgc caggaggag 
      aaggagttg actgatatg gaggtgtcg cttcaccga 
      acgaggagt cttctgggg gagaaggtt atcaggttg 
      aggcacttg gagcagtcc ttgcagagg gccaccatg 
      acggcggaa aaccgccaa gaagacgcc actctgagc 
      gacatttcc tcccacagt gccagttca gggataagc 
      tcgactgag ttcgccacc gctgacccc aaccccggg 
      aagagtttc atccacggg gagcacctc caagaatct 
      tccgaaata atccaaagc ttggagaac ttgaattcg 
      gaaattcgc gaaatctgg gatgtttta cgcaaacag 
      caacaacaa ggtgggatt tcggtaata ccaccccta 
      gtctaccct gacttaggt tggccgctg ttagcaggg 
      gctgccacc gttccggcg ccaccgtcc atccccacg 
      ttggccgat cgtttgcac aattaccgc caacatgtg 
      gcgctagct aacgctcaa agtacggta gtgacgcac 
      gctaatcaa ggggctact actacgctt gtcgaaaga 
      acgaggaat ttgaggcat tggctgagg caagcgggg 
      gttgatggg aatatggag ggtagtacg ccacaagca 
      acgctttga
    mRNA sequence
    • >TC007679 RA
      tgtctgttc cgacaagcg attattatg cgaagcctt 
      cgataatgt ccattgttt gcgaagaag tgttcgatg 
      agacgagcc acccttctg ctgaagaaa tccgggact 
      atgccacaa aaatcggga tcgacccgg actccgagc 
      cccagctgc tcccgcttg cagccgagg gcctcatga 
      aggctttgc cccccggtt ggagacctt gctatgacg 
      acaaatcaa aatcatact actactaca acaacaaca 
      cgggaaaaa cacaatggg agcaccctc tcgacgacg 
      tttaccgcg gtttggtga aaaaagtcc gtgccgaaa 
      gccaatctc taagtcttg gtgaaccca ccgaagatg 
      caacttatg ccagagatg aaattccaa gttttgagg 
      agcctccaa aactcacca aactcaaac tttcgccac 
      tcaaaacca gccccaagc acgaagcca aactcctca 
      aacaacgct cggaggagc atttacttt cgtcgcgga 
      aaattagtc tcaatattt tcaatagtt tcgatgaga 
      aaagcaatt ttgagaaac atccacgtg tgttggata 
      aaccagaat tgaagataa gtggtggag gatcaatgt 
      ttctgaagt gtaacacaa agaaaggcg ctgatttgg 
      ccccaagtc cggcaaaga gtgattccc cggatgtgc 
      aaaaccaac caaggagta tcctcaggg aacgcaatt 
      ccttggaag tctcccgga gtatggatt ttgataagg 
      tttctttag acaagtcgg agaaggacg atgatgata 
      agaaaagtg tgaggttta acctcgata cgaacaccg 
      atgttggct ttacatttt cggataaat caacttcca 
      gtgatgatg acataggga atgacatca tcaatgtta 
      aagtgactc caaataagc caaaatcac gatttaccg 
      taagcccgg tccccgact taacgaacg aaaagaaac 
      taattaagc caaacccaa ccgacttta ttaaaccaa 
      aattaacaa taagtaaag gttccgact ccgaagatg 
      actctctta aaagtaacg gttcggacg aaagtcctc 
      tccacacca aaatgttga tcaaatcga agaaactag 
      aggaaaaag ccgagaaaa aaattgccc aaatgaaac 
      aaaacatct gggaggaga aaaacgatg agttggtga 
      aattccgtg acgacttac aagagtcac aaaaggagg 
      aacttgaac gcattttaa ttaatgcta aaagtgacc 
      acgaagatc ggatcaaat cagagttgg agaatttgc 
      gaattgaga tggaaaatc gcaacaaag gggcgctaa 
      acgaggaaa ggatcaagt ttgagaagt tattggaag 
      agacgaggg gaaagtttg aaaaagatt ttgaacaag 
      aacaatcgc aaatgaagg aaaagttcg aggcaaaga 
      aagaagagt tggagaagt actacgagg agaaattgg 
      tcgaaatcg agaaagact tggccgaaa gaatggaga 
      aaaatcgtg acgagatga ttctaacgc acaactcaa 
      acgtcgaac aattgaaac agaaccatg ctataatta 
      tcgaggagt tgaaaaaag ggttcaagg ctgaggaag 
      atgccttga gacgggacc acaaggctg tgattgagg 
      aactaaaga ctagtaaag tagacgata ggaaagacg 
      ataagtttg aaaaaatcc gttgtgaga aacgactgc 
      tagaagata agtaccgat gcttgaagg agaaatatt 
      tgagactga aaaccgacg tgaaaatgt caatcgaga 
      agcggaaca aaaggaagg aacaaagcg gggcgacaa 
      caaccacag ggtcggaaa cggaacgca gtcactcca 
      acaacaaag acaggactc cgctgaatt cccccatac 
      gtaaacccc cccgttctg agacgaaaa aacccgagc 
      accgagcaa cccgccaac taatcatcc aagacctgg 
      acacttcaa tcagcgata aaagcgaaa ataaaaaag 
      attgtgatt cgagtgact ccacaaatc caggtcgca 
      gccgaaaaa aaattttct cccgcttga aaagctcct 
      caacttcga ggatcaaca cgggatcga aatcacgtc 
      gtaaccaac gttcgtgct cccctgttg agaatttgc 
      ggcgacagt tgcagaaat tggaggatt tggaggacc 
      agttccctc aaaatccac aagctgata cgtatcatt 
      tgaggatac acttggagc gggattcga ttaaaaggg 
      ccaaagaga gtttgagga atcagaaat cgctcttcc 
      agcagagac agaaagaat tgaagttga aacacgggt 
      ctatggcca ggcacacgt tgcaacagt tgtgccagg 
      aggagaagg agttgactg atatggagg tgtcgcttc 
      accgaacga ggagtcttc tgggggaga aggttatca 
      ggttgaggc acttggagc agtccttgc agagggcca 
      ccatgacgg cggaaaacc gccaagaag acgccactc 
      tgagcgaca tttcctccc acagtgcca gttcaggga 
      taagctcga ctgagttcg ccaccgctg accccaacc 
      ccgggaaga gtttcatcc acggggagc acctccaag 
      aatcttccg aaataatcc aaagcttgg agaacttga 
      attcggaaa ttcgcgaaa tctgggatg ttttacgca 
      aacagcaac aacaaggtg ggatttcgg taataccac 
      ccctagtct accctgact taggttggc cgctgttag 
      caggggctg ccaccgttc cggcgccac cgtccatcc 
      ccacgttgg ccgatcgtt tgcacaatt accgccaac 
      atgtggcgc tagctaacg ctcaaagta cggtagtga 
      cgcacgcta atcaagggg ctactacta cgcttgtcg 
      aaagaacga ggaatttga ggcattggc tgaggcaag 
      cgggggttg atgggaata tggagggta gtacgccac 
      aagcaacgc tttgaaatg ttgaacagt acaaatttt 
      gttggggtt tttagattt tatcggccg ggaaggcca 
      tttgttagg atagtcaag gccaattca aataaaact 
      ttattgttt ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY