Status
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Gene information
General information
TC007679 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 970017..973953
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007679
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01241

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007679 RA
      MSIVCEEVF DETSHPSAE EIRDYATKI GIDPDSEPQ 
LLPLAAEGL MKALPPGWR PCYDDKSKS YYYYNNNTG 
KTQWEHPLD DVYRGLVKK VRAESQSLS LGEPTEDAT 
YARDEIPSF EEPPKLTKL KLSPLKTSP KHEAKLLKQ 
RSEEHLLSS RKISLNIFN SFDEKSNFE KHPRVLDKP 
ELKISGGGS MFLKCNTKK GADLAPSPA KSDSPDVQN 
QPRSILRER NSLEVSRSM DFDKVSLDK SEKDDDDKK 
SVRFNLDTN TDVGFTFSD KSTSSDDDI GNDIINVKV 
TPNKPKSRF TVSPVPDLT NEKKLIKPN PTDFIKPKL 
TISKGSDSE DDSLKSNGS DESPLHTKM LIKSKKLEE 
KAEKKIAQM KQNIWEEKN DELVKFRDD LQESQKEEL 
ERILINAKS DHEDRIKSE LENLRIEME NRNKGALNE 
ERIKFEKLL EETRGKFEK DFEQEQSQM KEKFEAKKE 
ELEKYYEEK LVEIEKDLA ERMEKNRDE MILTHNSNV 
EQLKQNHAI IIEELKKGF KAEEDALRR DHKAVIEEL 
KTSKVDDRK DDKFEKIRC EKRLLEDKY RCLKEKYLR 
LKTDVKMSI EKRNKRKEQ SGATTTTGS ETERSHSNN 
KDRTPLNSP IRKPPRSET KKPEHRATR QLIIQDLDT 
SISDKSENK KDCDSSDST NPGRSRKKI FSRLKSSST 
SRINTGSKS RRNQRSCSP VENLRRQLQ KLEDLEDQF 
PQNPQADTY HLRIHLERD SIKRAKESL RNQKSLFQQ 
RQKELKLKH GSMARHTLQ QLCQEEKEL TDMEVSLHR 
TRSLLGEKV IRLRHLEQS LQRATMTAE NRQEDATLS 
DISSHSASS GISSTEFAT ADPNPGKSF IHGEHLQES 
SEIIQSLEN LNSEIREIW DVLRKQQQQ GGISVIPPL 
VYPDLGWPL LAGAATVPA PPSIPTLAD RLHNYRQHV 
ALANAQSTV VTHANQGAT TTLVERTRN LRHWLRQAG 
VDGNMEGST PQATL
    CDS sequence
    • >TC007679 RA
      atgtccatt gtttgcgaa gaagtgttc gatgagacg 
agccaccct tctgctgaa gaaatccgg gactatgcc 
acaaaaatc gggatcgac ccggactcc gagccccag 
ctgctcccg cttgcagcc gagggcctc atgaaggct 
ttgcccccc ggttggaga ccttgctat gacgacaaa 
tcaaaatca tactactac tacaacaac aacacggga 
aaaacacaa tgggagcac cctctcgac gacgtttac 
cgcggtttg gtgaaaaaa gtccgtgcc gaaagccaa 
tctctaagt cttggtgaa cccaccgaa gatgcaact 
tatgccaga gatgaaatt ccaagtttt gaggagcct 
ccaaaactc accaaactc aaactttcg ccactcaaa 
accagcccc aagcacgaa gccaaactc ctcaaacaa 
cgctcggag gagcattta ctttcgtcg cggaaaatt 
agtctcaat attttcaat agtttcgat gagaaaagc 
aattttgag aaacatcca cgtgtgttg gataaacca 
gaattgaag ataagtggt ggaggatca atgtttctg 
aagtgtaac acaaagaaa ggcgctgat ttggcccca 
agtccggca aagagtgat tccccggat gtgcaaaac 
caaccaagg agtatcctc agggaacgc aattccttg 
gaagtctcc cggagtatg gattttgat aaggtttct 
ttagacaag tcggagaag gacgatgat gataagaaa 
agtgtgagg tttaacctc gatacgaac accgatgtt 
ggctttaca ttttcggat aaatcaact tccagtgat 
gatgacata gggaatgac atcatcaat gttaaagtg 
actccaaat aagccaaaa tcacgattt accgtaagc 
ccggtcccc gacttaacg aacgaaaag aaactaatt 
aagccaaac ccaaccgac tttattaaa ccaaaatta 
acaataagt aaaggttcc gactccgaa gatgactct 
cttaaaagt aacggttcg gacgaaagt cctctccac 
accaaaatg ttgatcaaa tcgaagaaa ctagaggaa 
aaagccgag aaaaaaatt gcccaaatg aaacaaaac 
atctgggag gagaaaaac gatgagttg gtgaaattc 
cgtgacgac ttacaagag tcacaaaag gaggaactt 
gaacgcatt ttaattaat gctaaaagt gaccacgaa 
gatcggatc aaatcagag ttggagaat ttgcgaatt 
gagatggaa aatcgcaac aaaggggcg ctaaacgag 
gaaaggatc aagtttgag aagttattg gaagagacg 
aggggaaag tttgaaaaa gattttgaa caagaacaa 
tcgcaaatg aaggaaaag ttcgaggca aagaaagaa 
gagttggag aagtactac gaggagaaa ttggtcgaa 
atcgagaaa gacttggcc gaaagaatg gagaaaaat 
cgtgacgag atgattcta acgcacaac tcaaacgtc 
gaacaattg aaacagaac catgctata attatcgag 
gagttgaaa aaagggttc aaggctgag gaagatgcc 
ttgagacgg gaccacaag gctgtgatt gaggaacta 
aagactagt aaagtagac gataggaaa gacgataag 
tttgaaaaa atccgttgt gagaaacga ctgctagaa 
gataagtac cgatgcttg aaggagaaa tatttgaga 
ctgaaaacc gacgtgaaa atgtcaatc gagaagcgg 
aacaaaagg aaggaacaa agcggggcg acaacaacc 
acagggtcg gaaacggaa cgcagtcac tccaacaac 
aaagacagg actccgctg aattccccc atacgtaaa 
cccccccgt tctgagacg aaaaaaccc gagcaccga 
gcaacccgc caactaatc atccaagac ctggacact 
tcaatcagc gataaaagc gaaaataaa aaagattgt 
gattcgagt gactccaca aatccaggt cgcagccga 
aaaaaaatt ttctcccgc ttgaaaagc tcctcaact 
tcgaggatc aacacggga tcgaaatca cgtcgtaac 
caacgttcg tgctcccct gttgagaat ttgcggcga 
cagttgcag aaattggag gatttggag gaccagttc 
cctcaaaat ccacaagct gatacgtat catttgagg 
atacacttg gagcgggat tcgattaaa agggccaaa 
gagagtttg aggaatcag aaatcgctc ttccagcag 
agacagaaa gaattgaag ttgaaacac gggtctatg 
gccaggcac acgttgcaa cagttgtgc caggaggag 
aaggagttg actgatatg gaggtgtcg cttcaccga 
acgaggagt cttctgggg gagaaggtt atcaggttg 
aggcacttg gagcagtcc ttgcagagg gccaccatg 
acggcggaa aaccgccaa gaagacgcc actctgagc 
gacatttcc tcccacagt gccagttca gggataagc 
tcgactgag ttcgccacc gctgacccc aaccccggg 
aagagtttc atccacggg gagcacctc caagaatct 
tccgaaata atccaaagc ttggagaac ttgaattcg 
gaaattcgc gaaatctgg gatgtttta cgcaaacag 
caacaacaa ggtgggatt tcggtaata ccaccccta 
gtctaccct gacttaggt tggccgctg ttagcaggg 
gctgccacc gttccggcg ccaccgtcc atccccacg 
ttggccgat cgtttgcac aattaccgc caacatgtg 
gcgctagct aacgctcaa agtacggta gtgacgcac 
gctaatcaa ggggctact actacgctt gtcgaaaga 
acgaggaat ttgaggcat tggctgagg caagcgggg 
gttgatggg aatatggag ggtagtacg ccacaagca 
acgctttga
    mRNA sequence
    • >TC007679 RA
      tgtctgttc cgacaagcg attattatg cgaagcctt 
cgataatgt ccattgttt gcgaagaag tgttcgatg 
agacgagcc acccttctg ctgaagaaa tccgggact 
atgccacaa aaatcggga tcgacccgg actccgagc 
cccagctgc tcccgcttg cagccgagg gcctcatga 
aggctttgc cccccggtt ggagacctt gctatgacg 
acaaatcaa aatcatact actactaca acaacaaca 
cgggaaaaa cacaatggg agcaccctc tcgacgacg 
tttaccgcg gtttggtga aaaaagtcc gtgccgaaa 
gccaatctc taagtcttg gtgaaccca ccgaagatg 
caacttatg ccagagatg aaattccaa gttttgagg 
agcctccaa aactcacca aactcaaac tttcgccac 
tcaaaacca gccccaagc acgaagcca aactcctca 
aacaacgct cggaggagc atttacttt cgtcgcgga 
aaattagtc tcaatattt tcaatagtt tcgatgaga 
aaagcaatt ttgagaaac atccacgtg tgttggata 
aaccagaat tgaagataa gtggtggag gatcaatgt 
ttctgaagt gtaacacaa agaaaggcg ctgatttgg 
ccccaagtc cggcaaaga gtgattccc cggatgtgc 
aaaaccaac caaggagta tcctcaggg aacgcaatt 
ccttggaag tctcccgga gtatggatt ttgataagg 
tttctttag acaagtcgg agaaggacg atgatgata 
agaaaagtg tgaggttta acctcgata cgaacaccg 
atgttggct ttacatttt cggataaat caacttcca 
gtgatgatg acataggga atgacatca tcaatgtta 
aagtgactc caaataagc caaaatcac gatttaccg 
taagcccgg tccccgact taacgaacg aaaagaaac 
taattaagc caaacccaa ccgacttta ttaaaccaa 
aattaacaa taagtaaag gttccgact ccgaagatg 
actctctta aaagtaacg gttcggacg aaagtcctc 
tccacacca aaatgttga tcaaatcga agaaactag 
aggaaaaag ccgagaaaa aaattgccc aaatgaaac 
aaaacatct gggaggaga aaaacgatg agttggtga 
aattccgtg acgacttac aagagtcac aaaaggagg 
aacttgaac gcattttaa ttaatgcta aaagtgacc 
acgaagatc ggatcaaat cagagttgg agaatttgc 
gaattgaga tggaaaatc gcaacaaag gggcgctaa 
acgaggaaa ggatcaagt ttgagaagt tattggaag 
agacgaggg gaaagtttg aaaaagatt ttgaacaag 
aacaatcgc aaatgaagg aaaagttcg aggcaaaga 
aagaagagt tggagaagt actacgagg agaaattgg 
tcgaaatcg agaaagact tggccgaaa gaatggaga 
aaaatcgtg acgagatga ttctaacgc acaactcaa 
acgtcgaac aattgaaac agaaccatg ctataatta 
tcgaggagt tgaaaaaag ggttcaagg ctgaggaag 
atgccttga gacgggacc acaaggctg tgattgagg 
aactaaaga ctagtaaag tagacgata ggaaagacg 
ataagtttg aaaaaatcc gttgtgaga aacgactgc 
tagaagata agtaccgat gcttgaagg agaaatatt 
tgagactga aaaccgacg tgaaaatgt caatcgaga 
agcggaaca aaaggaagg aacaaagcg gggcgacaa 
caaccacag ggtcggaaa cggaacgca gtcactcca 
acaacaaag acaggactc cgctgaatt cccccatac 
gtaaacccc cccgttctg agacgaaaa aacccgagc 
accgagcaa cccgccaac taatcatcc aagacctgg 
acacttcaa tcagcgata aaagcgaaa ataaaaaag 
attgtgatt cgagtgact ccacaaatc caggtcgca 
gccgaaaaa aaattttct cccgcttga aaagctcct 
caacttcga ggatcaaca cgggatcga aatcacgtc 
gtaaccaac gttcgtgct cccctgttg agaatttgc 
ggcgacagt tgcagaaat tggaggatt tggaggacc 
agttccctc aaaatccac aagctgata cgtatcatt 
tgaggatac acttggagc gggattcga ttaaaaggg 
ccaaagaga gtttgagga atcagaaat cgctcttcc 
agcagagac agaaagaat tgaagttga aacacgggt 
ctatggcca ggcacacgt tgcaacagt tgtgccagg 
aggagaagg agttgactg atatggagg tgtcgcttc 
accgaacga ggagtcttc tgggggaga aggttatca 
ggttgaggc acttggagc agtccttgc agagggcca 
ccatgacgg cggaaaacc gccaagaag acgccactc 
tgagcgaca tttcctccc acagtgcca gttcaggga 
taagctcga ctgagttcg ccaccgctg accccaacc 
ccgggaaga gtttcatcc acggggagc acctccaag 
aatcttccg aaataatcc aaagcttgg agaacttga 
attcggaaa ttcgcgaaa tctgggatg ttttacgca 
aacagcaac aacaaggtg ggatttcgg taataccac 
ccctagtct accctgact taggttggc cgctgttag 
caggggctg ccaccgttc cggcgccac cgtccatcc 
ccacgttgg ccgatcgtt tgcacaatt accgccaac 
atgtggcgc tagctaacg ctcaaagta cggtagtga 
cgcacgcta atcaagggg ctactacta cgcttgtcg 
aaagaacga ggaatttga ggcattggc tgaggcaag 
cgggggttg atgggaata tggagggta gtacgccac 
aagcaacgc tttgaaatg ttgaacagt acaaatttt 
gttggggtt tttagattt tatcggccg ggaaggcca 
tttgttagg atagtcaag gccaattca aataaaact 
ttattgttt ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY