General information | |||
TC007679 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 970017..973953 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC007679 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01241 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
legs sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC007679 RA
MSIVCEEVF DETSHPSAE EIRDYATKI GIDPDSEPQ LLPLAAEGL MKALPPGWR PCYDDKSKS YYYYNNNTG KTQWEHPLD DVYRGLVKK VRAESQSLS LGEPTEDAT YARDEIPSF EEPPKLTKL KLSPLKTSP KHEAKLLKQ RSEEHLLSS RKISLNIFN SFDEKSNFE KHPRVLDKP ELKISGGGS MFLKCNTKK GADLAPSPA KSDSPDVQN QPRSILRER NSLEVSRSM DFDKVSLDK SEKDDDDKK SVRFNLDTN TDVGFTFSD KSTSSDDDI GNDIINVKV TPNKPKSRF TVSPVPDLT NEKKLIKPN PTDFIKPKL TISKGSDSE DDSLKSNGS DESPLHTKM LIKSKKLEE KAEKKIAQM KQNIWEEKN DELVKFRDD LQESQKEEL ERILINAKS DHEDRIKSE LENLRIEME NRNKGALNE ERIKFEKLL EETRGKFEK DFEQEQSQM KEKFEAKKE ELEKYYEEK LVEIEKDLA ERMEKNRDE MILTHNSNV EQLKQNHAI IIEELKKGF KAEEDALRR DHKAVIEEL KTSKVDDRK DDKFEKIRC EKRLLEDKY RCLKEKYLR LKTDVKMSI EKRNKRKEQ SGATTTTGS ETERSHSNN KDRTPLNSP IRKPPRSET KKPEHRATR QLIIQDLDT SISDKSENK KDCDSSDST NPGRSRKKI FSRLKSSST SRINTGSKS RRNQRSCSP VENLRRQLQ KLEDLEDQF PQNPQADTY HLRIHLERD SIKRAKESL RNQKSLFQQ RQKELKLKH GSMARHTLQ QLCQEEKEL TDMEVSLHR TRSLLGEKV IRLRHLEQS LQRATMTAE NRQEDATLS DISSHSASS GISSTEFAT ADPNPGKSF IHGEHLQES SEIIQSLEN LNSEIREIW DVLRKQQQQ GGISVIPPL VYPDLGWPL LAGAATVPA PPSIPTLAD RLHNYRQHV ALANAQSTV VTHANQGAT TTLVERTRN LRHWLRQAG VDGNMEGST PQATL
- >TC007679 RA
atgtccatt gtttgcgaa gaagtgttc gatgagacg agccaccct tctgctgaa gaaatccgg gactatgcc acaaaaatc gggatcgac ccggactcc gagccccag ctgctcccg cttgcagcc gagggcctc atgaaggct ttgcccccc ggttggaga ccttgctat gacgacaaa tcaaaatca tactactac tacaacaac aacacggga aaaacacaa tgggagcac cctctcgac gacgtttac cgcggtttg gtgaaaaaa gtccgtgcc gaaagccaa tctctaagt cttggtgaa cccaccgaa gatgcaact tatgccaga gatgaaatt ccaagtttt gaggagcct ccaaaactc accaaactc aaactttcg ccactcaaa accagcccc aagcacgaa gccaaactc ctcaaacaa cgctcggag gagcattta ctttcgtcg cggaaaatt agtctcaat attttcaat agtttcgat gagaaaagc aattttgag aaacatcca cgtgtgttg gataaacca gaattgaag ataagtggt ggaggatca atgtttctg aagtgtaac acaaagaaa ggcgctgat ttggcccca agtccggca aagagtgat tccccggat gtgcaaaac caaccaagg agtatcctc agggaacgc aattccttg gaagtctcc cggagtatg gattttgat aaggtttct ttagacaag tcggagaag gacgatgat gataagaaa agtgtgagg tttaacctc gatacgaac accgatgtt ggctttaca ttttcggat aaatcaact tccagtgat gatgacata gggaatgac atcatcaat gttaaagtg actccaaat aagccaaaa tcacgattt accgtaagc ccggtcccc gacttaacg aacgaaaag aaactaatt aagccaaac ccaaccgac tttattaaa ccaaaatta acaataagt aaaggttcc gactccgaa gatgactct cttaaaagt aacggttcg gacgaaagt cctctccac accaaaatg ttgatcaaa tcgaagaaa ctagaggaa aaagccgag aaaaaaatt gcccaaatg aaacaaaac atctgggag gagaaaaac gatgagttg gtgaaattc cgtgacgac ttacaagag tcacaaaag gaggaactt gaacgcatt ttaattaat gctaaaagt gaccacgaa gatcggatc aaatcagag ttggagaat ttgcgaatt gagatggaa aatcgcaac aaaggggcg ctaaacgag gaaaggatc aagtttgag aagttattg gaagagacg aggggaaag tttgaaaaa gattttgaa caagaacaa tcgcaaatg aaggaaaag ttcgaggca aagaaagaa gagttggag aagtactac gaggagaaa ttggtcgaa atcgagaaa gacttggcc gaaagaatg gagaaaaat cgtgacgag atgattcta acgcacaac tcaaacgtc gaacaattg aaacagaac catgctata attatcgag gagttgaaa aaagggttc aaggctgag gaagatgcc ttgagacgg gaccacaag gctgtgatt gaggaacta aagactagt aaagtagac gataggaaa gacgataag tttgaaaaa atccgttgt gagaaacga ctgctagaa gataagtac cgatgcttg aaggagaaa tatttgaga ctgaaaacc gacgtgaaa atgtcaatc gagaagcgg aacaaaagg aaggaacaa agcggggcg acaacaacc acagggtcg gaaacggaa cgcagtcac tccaacaac aaagacagg actccgctg aattccccc atacgtaaa cccccccgt tctgagacg aaaaaaccc gagcaccga gcaacccgc caactaatc atccaagac ctggacact tcaatcagc gataaaagc gaaaataaa aaagattgt gattcgagt gactccaca aatccaggt cgcagccga aaaaaaatt ttctcccgc ttgaaaagc tcctcaact tcgaggatc aacacggga tcgaaatca cgtcgtaac caacgttcg tgctcccct gttgagaat ttgcggcga cagttgcag aaattggag gatttggag gaccagttc cctcaaaat ccacaagct gatacgtat catttgagg atacacttg gagcgggat tcgattaaa agggccaaa gagagtttg aggaatcag aaatcgctc ttccagcag agacagaaa gaattgaag ttgaaacac gggtctatg gccaggcac acgttgcaa cagttgtgc caggaggag aaggagttg actgatatg gaggtgtcg cttcaccga acgaggagt cttctgggg gagaaggtt atcaggttg aggcacttg gagcagtcc ttgcagagg gccaccatg acggcggaa aaccgccaa gaagacgcc actctgagc gacatttcc tcccacagt gccagttca gggataagc tcgactgag ttcgccacc gctgacccc aaccccggg aagagtttc atccacggg gagcacctc caagaatct tccgaaata atccaaagc ttggagaac ttgaattcg gaaattcgc gaaatctgg gatgtttta cgcaaacag caacaacaa ggtgggatt tcggtaata ccaccccta gtctaccct gacttaggt tggccgctg ttagcaggg gctgccacc gttccggcg ccaccgtcc atccccacg ttggccgat cgtttgcac aattaccgc caacatgtg gcgctagct aacgctcaa agtacggta gtgacgcac gctaatcaa ggggctact actacgctt gtcgaaaga acgaggaat ttgaggcat tggctgagg caagcgggg gttgatggg aatatggag ggtagtacg ccacaagca acgctttga
- >TC007679 RA
tgtctgttc cgacaagcg attattatg cgaagcctt cgataatgt ccattgttt gcgaagaag tgttcgatg agacgagcc acccttctg ctgaagaaa tccgggact atgccacaa aaatcggga tcgacccgg actccgagc cccagctgc tcccgcttg cagccgagg gcctcatga aggctttgc cccccggtt ggagacctt gctatgacg acaaatcaa aatcatact actactaca acaacaaca cgggaaaaa cacaatggg agcaccctc tcgacgacg tttaccgcg gtttggtga aaaaagtcc gtgccgaaa gccaatctc taagtcttg gtgaaccca ccgaagatg caacttatg ccagagatg aaattccaa gttttgagg agcctccaa aactcacca aactcaaac tttcgccac tcaaaacca gccccaagc acgaagcca aactcctca aacaacgct cggaggagc atttacttt cgtcgcgga aaattagtc tcaatattt tcaatagtt tcgatgaga aaagcaatt ttgagaaac atccacgtg tgttggata aaccagaat tgaagataa gtggtggag gatcaatgt ttctgaagt gtaacacaa agaaaggcg ctgatttgg ccccaagtc cggcaaaga gtgattccc cggatgtgc aaaaccaac caaggagta tcctcaggg aacgcaatt ccttggaag tctcccgga gtatggatt ttgataagg tttctttag acaagtcgg agaaggacg atgatgata agaaaagtg tgaggttta acctcgata cgaacaccg atgttggct ttacatttt cggataaat caacttcca gtgatgatg acataggga atgacatca tcaatgtta aagtgactc caaataagc caaaatcac gatttaccg taagcccgg tccccgact taacgaacg aaaagaaac taattaagc caaacccaa ccgacttta ttaaaccaa aattaacaa taagtaaag gttccgact ccgaagatg actctctta aaagtaacg gttcggacg aaagtcctc tccacacca aaatgttga tcaaatcga agaaactag aggaaaaag ccgagaaaa aaattgccc aaatgaaac aaaacatct gggaggaga aaaacgatg agttggtga aattccgtg acgacttac aagagtcac aaaaggagg aacttgaac gcattttaa ttaatgcta aaagtgacc acgaagatc ggatcaaat cagagttgg agaatttgc gaattgaga tggaaaatc gcaacaaag gggcgctaa acgaggaaa ggatcaagt ttgagaagt tattggaag agacgaggg gaaagtttg aaaaagatt ttgaacaag aacaatcgc aaatgaagg aaaagttcg aggcaaaga aagaagagt tggagaagt actacgagg agaaattgg tcgaaatcg agaaagact tggccgaaa gaatggaga aaaatcgtg acgagatga ttctaacgc acaactcaa acgtcgaac aattgaaac agaaccatg ctataatta tcgaggagt tgaaaaaag ggttcaagg ctgaggaag atgccttga gacgggacc acaaggctg tgattgagg aactaaaga ctagtaaag tagacgata ggaaagacg ataagtttg aaaaaatcc gttgtgaga aacgactgc tagaagata agtaccgat gcttgaagg agaaatatt tgagactga aaaccgacg tgaaaatgt caatcgaga agcggaaca aaaggaagg aacaaagcg gggcgacaa caaccacag ggtcggaaa cggaacgca gtcactcca acaacaaag acaggactc cgctgaatt cccccatac gtaaacccc cccgttctg agacgaaaa aacccgagc accgagcaa cccgccaac taatcatcc aagacctgg acacttcaa tcagcgata aaagcgaaa ataaaaaag attgtgatt cgagtgact ccacaaatc caggtcgca gccgaaaaa aaattttct cccgcttga aaagctcct caacttcga ggatcaaca cgggatcga aatcacgtc gtaaccaac gttcgtgct cccctgttg agaatttgc ggcgacagt tgcagaaat tggaggatt tggaggacc agttccctc aaaatccac aagctgata cgtatcatt tgaggatac acttggagc gggattcga ttaaaaggg ccaaagaga gtttgagga atcagaaat cgctcttcc agcagagac agaaagaat tgaagttga aacacgggt ctatggcca ggcacacgt tgcaacagt tgtgccagg aggagaagg agttgactg atatggagg tgtcgcttc accgaacga ggagtcttc tgggggaga aggttatca ggttgaggc acttggagc agtccttgc agagggcca ccatgacgg cggaaaacc gccaagaag acgccactc tgagcgaca tttcctccc acagtgcca gttcaggga taagctcga ctgagttcg ccaccgctg accccaacc ccgggaaga gtttcatcc acggggagc acctccaag aatcttccg aaataatcc aaagcttgg agaacttga attcggaaa ttcgcgaaa tctgggatg ttttacgca aacagcaac aacaaggtg ggatttcgg taataccac ccctagtct accctgact taggttggc cgctgttag caggggctg ccaccgttc cggcgccac cgtccatcc ccacgttgg ccgatcgtt tgcacaatt accgccaac atgtggcgc tagctaacg ctcaaagta cggtagtga cgcacgcta atcaagggg ctactacta cgcttgtcg aaagaacga ggaatttga ggcattggc tgaggcaag cgggggttg atgggaata tggagggta gtacgccac aagcaacgc tttgaaatg ttgaacagt acaaatttt gttggggtt tttagattt tatcggccg ggaaggcca tttgttagg atagtcaag gccaattca aataaaact ttattgttt ca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
