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Gene information
General information
TC007676 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 1032825..1038605
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007676
    iBeetle screen iB_01240
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007676 RA
      MQCEEVEAP APEMTFNDS RESPETKTA AQILEDSKF 
      DSRKFEEIN LEDDIEKVE VSSGASSET NEEEFVEPA 
      QHLLPEQNP NESIKSWLQ RITDLQKQL SFDPPPGPF 
      PLKPSPQPT PNRVVKFQD LPYMGEMTL DNSKPRRGR 
      KPKKADICH LIYKNYGTI FPGTPNPKS YLEKETNKK 
      SEEPANRSD VQNRIISSL LEKRLTQEI KKTDVVVEK 
      SDQNEPLNL CIRDLNHLK IRLLKKNEL KSEPGSDDD 
      VEFVHETPS PGKPEPLGT PPEAPGGYV YWPNAGVFI 
      HPMALQQQL MYYQRMAAN NSYTLPSHS PRPPPPDPE 
      PELRKITPK ARKKPEETE PQPEQKTKR NASTSSDKT 
      PTKRKRSAI FIPPIPPEN NTNSATEVS ICKFKFTGG 
      AKPSLQEKK MLSVDSGGN FRYYSGTGD KSMRGYEFF 
      PRETLQQQI NNSQGSSTG AFLQASGEK IYPVAPVDF 
      TGTKPSQEF LLAPEAPPT PDLHRHKKR KSRKSLQRE 
      KLEQTFKEK GFLIQTQQL ESAEGATYC KFRQLRKFT 
      RYLFRSWKD YLPGNVREL QEGEKPDEE DGDNLSRHS 
      SELQ
    • >TC007676 RB
      MNEKGVNMQ CEEVEAPAP EMTFNDSRE SPETKTAAQ 
      ILEDSKFDS RKFEEINLE DDIEKVEVS SGASSETNE 
      EEFVEPAQH LLPEQNPNE SIKSWLQRI TDLQKQLSF 
      DPPPGPFPL KPSPQPTPN RVVKFQDLP YMGEMTLDN 
      SKPRRGRKP KKADICHLI YKNYGTIFP GTPNPKSYL 
      EKETNKKSE EPANRSDVQ NRIISSLLE KRLTQEIKK 
      TDVVVEKSD QNEPLNLCI RDLNHLKIR LLKKNELKS 
      EPGSDDDVE FVHETPSPG KPEPLGTPP EAPGGYVYW 
      PNAGVFIHP MALQQQLMY YQRMAANNS YTLPSHSPR 
      PPPPDPEPE LRKITPKAR KKPEETEPQ PEQKTKRNA 
      STSSDKTPT KRKRSAIFI PPIPPENNT NSATEVSIC 
      KFKFTGGAK PSLQEKKML SVDSGGNFR YYSGTGDKS 
      MRGYEFFPR ETLQQQINN SQGSSTGAF LQASGEKIY 
      PVAPVDFTG TKPSQEFLL APEAPPTPD LHRHKKRKS 
      RKSLQREKL EQTFKEKGF LIQTQQLES AEGATYCKF 
      RQLRKFTRY LFRSWKDYL PGNVRELQE GEKPDEEDG 
      DNLSRHSSE LQ
    CDS sequence
    • >TC007676 RA
      atgcaatgc gaagaagta gaggcccct gcaccggaa 
      atgaccttc aatgacagc agagaatcg ccggaaacc 
      aagacggct gctcaaatt ttagaagat tccaagttc 
      gactctcgc aaatttgaa gaaatcaac cttgaagat 
      gacatcgag aaagtcgag gtatcttct ggagcttcc 
      tctgagaca aacgaagag gagtttgtt gagccagct 
      caacacctg ctcccggag caaaacccc aacgaatca 
      atcaaaagt tggctacaa agaatcacc gacctgcag 
      aaacaacta tctttcgac ccacctcct ggacctttc 
      cccctcaaa ccatcacca cagccaacc ccaaaccgc 
      gtggtcaaa ttccaggac ctcccctac atgggcgaa 
      atgacccta gacaattcc aaaccacgc cgtggtcga 
      aaaccgaaa aaagcggac atttgtcac ttaatttac 
      aaaaattac gggacgatt tttcccggc actcccaac 
      ccgaagagc tacttggaa aaagagacg aataaaaaa 
      agcgaagag cccgccaat cgaagtgac gtccaaaac 
      cggatcatc agtagcctt ctggagaaa agactgact 
      caggaaatc aaaaaaacg gacgttgtt gttgaaaaa 
      agtgaccaa aacgaaccg ttaaacttg tgcattcgt 
      gacttaaac cacttgaaa atccgtctt ttgaagaag 
      aacgaacta aagtcggag ccagggagt gacgacgac 
      gtggagttc gtgcacgaa accccatcc cctgggaag 
      cccgaaccc ttggggacg ccgcccgaa gccccaggt 
      ggttacgtc tactggccc aatgccggg gtgttcatc 
      cacccgatg gccctccag cagcagcta atgtactac 
      cagaggatg gcggccaac aacagctac accctcccc 
      agccacagc ccgcggccc ccgcccccc gaccccgag 
      cccgaactc aggaaaatc accccgaaa gcgcgcaaa 
      aaacccgaa gagactgag ccccaacct gagcaaaag 
      accaaacgc aacgcctcc acctcctcg gacaagacc 
      cccaccaag cggaaacgc tccgccatc ttcattcct 
      cccatcccc cccgaaaac aacacaaac tcagccacc 
      gaagtcagt atttgcaag tttaaattc accggaggt 
      gcgaaacct agtctacaa gaaaaaaaa atgctgtcg 
      gtcgattcc ggcggcaat tttcgctac tacagcgga 
      acgggggac aagtcaatg cgcggctac gagttcttc 
      ccgcgggaa accctccag cagcagatc aacaactcg 
      caaggctcc agcaccgga gcgttcctg caagcgagt 
      ggggagaag atttatccg gtggctccg gtcgatttc 
      accggaact aagccgagt caggagttt ctgctggct 
      ccggaagcg cccccaacg cctgacttg caccggcat 
      aagaaacgc aagtccagg aagtcgctc cagagggag 
      aagctggag cagacgttc aaggagaag gggtttctg 
      atacagacg cagcagctg gaatcggct gaaggcgct 
      acttattgc aagtttaga cagttgagg aagtttacg 
      aggtatttg ttcaggagt tggaaggat tatctgccg 
      gggaatgtg cgcgagttg caagagggg gaaaaaccg 
      gatgaggaa gatggtgat aatttatcc agacattcg 
      agtgagttg cagtga
    • >TC007676 RB
      atgaacgag aagggggtt aacatgcaa tgcgaagaa 
      gtagaggcc cctgcaccg gaaatgacc ttcaatgac 
      agcagagaa tcgccggaa accaagacg gctgctcaa 
      attttagaa gattccaag ttcgactct cgcaaattt 
      gaagaaatc aaccttgaa gatgacatc gagaaagtc 
      gaggtatct tctggagct tcctctgag acaaacgaa 
      gaggagttt gttgagcca gctcaacac ctgctcccg 
      gagcaaaac cccaacgaa tcaatcaaa agttggcta 
      caaagaatc accgacctg cagaaacaa ctatctttc 
      gacccacct cctggacct ttccccctc aaaccatca 
      ccacagcca accccaaac cgcgtggtc aaattccag 
      gacctcccc tacatgggc gaaatgacc ctagacaat 
      tccaaacca cgccgtggt cgaaaaccg aaaaaagcg 
      gacatttgt cacttaatt tacaaaaat tacgggacg 
      atttttccc ggcactccc aacccgaag agctacttg 
      gaaaaagag acgaataaa aaaagcgaa gagcccgcc 
      aatcgaagt gacgtccaa aaccggatc atcagtagc 
      cttctggag aaaagactg actcaggaa atcaaaaaa 
      acggacgtt gttgttgaa aaaagtgac caaaacgaa 
      ccgttaaac ttgtgcatt cgtgactta aaccacttg 
      aaaatccgt cttttgaag aagaacgaa ctaaagtcg 
      gagccaggg agtgacgac gacgtggag ttcgtgcac 
      gaaacccca tcccctggg aagcccgaa cccttgggg 
      acgccgccc gaagcccca ggtggttac gtctactgg 
      cccaatgcc ggggtgttc atccacccg atggccctc 
      cagcagcag ctaatgtac taccagagg atggcggcc 
      aacaacagc tacaccctc cccagccac agcccgcgg 
      cccccgccc cccgacccc gagcccgaa ctcaggaaa 
      atcaccccg aaagcgcgc aaaaaaccc gaagagact 
      gagccccaa cctgagcaa aagaccaaa cgcaacgcc 
      tccacctcc tcggacaag acccccacc aagcggaaa 
      cgctccgcc atcttcatt cctcccatc ccccccgaa 
      aacaacaca aactcagcc accgaagtc agtatttgc 
      aagtttaaa ttcaccgga ggtgcgaaa cctagtcta 
      caagaaaaa aaaatgctg tcggtcgat tccggcggc 
      aattttcgc tactacagc ggaacgggg gacaagtca 
      atgcgcggc tacgagttc ttcccgcgg gaaaccctc 
      cagcagcag atcaacaac tcgcaaggc tccagcacc 
      ggagcgttc ctgcaagcg agtggggag aagatttat 
      ccggtggct ccggtcgat ttcaccgga actaagccg 
      agtcaggag tttctgctg gctccggaa gcgccccca 
      acgcctgac ttgcaccgg cataagaaa cgcaagtcc 
      aggaagtcg ctccagagg gagaagctg gagcagacg 
      ttcaaggag aaggggttt ctgatacag acgcagcag 
      ctggaatcg gctgaaggc gctacttat tgcaagttt 
      agacagttg aggaagttt acgaggtat ttgttcagg 
      agttggaag gattatctg ccggggaat gtgcgcgag 
      ttgcaagag ggggaaaaa ccggatgag gaagatggt 
      gataattta tccagacat tcgagtgag ttgcagtga
    mRNA sequence
    • >TC007676 RA
      tcacaatgt gaggtgttt ttcgggtga ttatttcaa 
      ttagtgcca tggattatg tttacgaat ttaagaggg 
      ggttaacat gcaatgcga agaagtaga ggcccctgc 
      accggaaat gaccttcaa tgacagcag agaatcgcc 
      ggaaaccaa gacggctgc tcaaatttt agaagattc 
      caagttcga ctctcgcaa atttgaaga aatcaacct 
      tgaagatga catcgagaa agtcgaggt atcttctgg 
      agcttcctc tgagacaaa cgaagagga gtttgttga 
      gccagctca acacctgct cccggagca aaaccccaa 
      cgaatcaat caaaagttg gctacaaag aatcaccga 
      cctgcagaa acaactatc tttcgaccc acctcctgg 
      acctttccc cctcaaacc atcaccaca gccaacccc 
      aaaccgcgt ggtcaaatt ccaggacct cccctacat 
      gggcgaaat gaccctaga caattccaa accacgccg 
      tggtcgaaa accgaaaaa agcggacat ttgtcactt 
      aatttacaa aaattacgg gacgatttt tcccggcac 
      tcccaaccc gaagagcta cttggaaaa agagacgaa 
      taaaaaaag cgaagagcc cgccaatcg aagtgacgt 
      ccaaaaccg gatcatcag tagccttct ggagaaaag 
      actgactca ggaaatcaa aaaaacgga cgttgttgt 
      tgaaaaaag tgaccaaaa cgaaccgtt aaacttgtg 
      cattcgtga cttaaacca cttgaaaat ccgtctttt 
      gaagaagaa cgaactaaa gtcggagcc agggagtga 
      cgacgacgt ggagttcgt gcacgaaac cccatcccc 
      tgggaagcc cgaaccctt ggggacgcc gcccgaagc 
      cccaggtgg ttacgtcta ctggcccaa tgccggggt 
      gttcatcca cccgatggc cctccagca gcagctaat 
      gtactacca gaggatggc ggccaacaa cagctacac 
      cctccccag ccacagccc gcggccccc gccccccga 
      ccccgagcc cgaactcag gaaaatcac cccgaaagc 
      gcgcaaaaa acccgaaga gactgagcc ccaacctga 
      gcaaaagac caaacgcaa cgcctccac ctcctcgga 
      caagacccc caccaagcg gaaacgctc cgccatctt 
      cattcctcc catcccccc cgaaaacaa cacaaactc 
      agccaccga agtcagtat ttgcaagtt taaattcac 
      cggaggtgc gaaacctag tctacaaga aaaaaaaat 
      gctgtcggt cgattccgg cggcaattt tcgctacta 
      cagcggaac gggggacaa gtcaatgcg cggctacga 
      gttcttccc gcgggaaac cctccagca gcagatcaa 
      caactcgca aggctccag caccggagc gttcctgca 
      agcgagtgg ggagaagat ttatccggt ggctccggt 
      cgatttcac cggaactaa gccgagtca ggagtttct 
      gctggctcc ggaagcgcc cccaacgcc tgacttgca 
      ccggcataa gaaacgcaa gtccaggaa gtcgctcca 
      gagggagaa gctggagca gacgttcaa ggagaaggg 
      gtttctgat acagacgca gcagctgga atcggctga 
      aggcgctac ttattgcaa gtttagaca gttgaggaa 
      gtttacgag gtatttgtt caggagttg gaaggatta 
      tctgccggg gaatgtgcg cgagttgca agaggggga 
      aaaaccgga tgaggaaga tggtgataa tttatccag 
      acattcgag tgagttgca gtgaaacga ccaaagatt 
      gcgctgttt tgggacgct gagtggtgc taattattg 
      ataatctag tcgtaattc attaatgtt gttaatcgt 
      aagggaatt gttaataaa actcgctgt ttcac
    • >TC007676 RB
      cttctgggg acgatctga aacccattt gggacagtt 
      atgcgcacc ggcggatcc acggggtgg acaataaaa 
      aataaacag caaaccaaa taaaaaata aacattttt 
      attgataaa ttgagcctt gcaacagaa cggagacgt 
      tttcgcagt gcgacttca acttgtcac aatgtgagg 
      tgtttttcg ggtgattat ttcaattag tgccatgga 
      ttatgttta cgaatttaa ggcaagtgt gtttattga 
      ctcacgatt acaattaac attttttgc gtaacccag 
      tgttacttg cctcccctc cggcggcca ttacggtca 
      cgtgacggg gtagcaacc cgcctgctt tgttttttt 
      ttcgaaaat tgatctcga gcgcatatt ttcgtctcg 
      cttaaaaat accgcagct tcaattaat tgacaagaa 
      agtgcgctg tgagagaaa aaatgaacg catgctgtt 
      aatcccgaa aagcgacgg aaatgctcg gaggaacat 
      aagaacggg ttaataata aaccgagtg aggccggag 
      taccaaata tggttcaat ctcgtatta caccaacgg 
      ccaaattca gaatatacc acctaagag gagagaggg 
      cgtcccgac gccgaaaat agcgccgtc gcgcgcctt 
      ctacggcca aagtcggct tagcgctca cataataaa 
      taataacag agtgtgaac accacgaaa aattttgcg 
      tgtgcggga ttttttttt attgacaga atgcaacgg 
      aaatgaacg agaaggggg ttaacatgc aatgcgaag 
      aagtagagg cccctgcac cggaaatga ccttcaatg 
      acagcagag aatcgccgg aaaccaaga cggctgctc 
      aaattttag aagattcca agttcgact ctcgcaaat 
      ttgaagaaa tcaaccttg aagatgaca tcgagaaag 
      tcgaggtat cttctggag cttcctctg agacaaacg 
      aagaggagt ttgttgagc cagctcaac acctgctcc 
      cggagcaaa accccaacg aatcaatca aaagttggc 
      tacaaagaa tcaccgacc tgcagaaac aactatctt 
      tcgacccac ctcctggac ctttccccc tcaaaccat 
      caccacagc caaccccaa accgcgtgg tcaaattcc 
      aggacctcc cctacatgg gcgaaatga ccctagaca 
      attccaaac cacgccgtg gtcgaaaac cgaaaaaag 
      cggacattt gtcacttaa tttacaaaa attacggga 
      cgatttttc ccggcactc ccaacccga agagctact 
      tggaaaaag agacgaata aaaaaagcg aagagcccg 
      ccaatcgaa gtgacgtcc aaaaccgga tcatcagta 
      gccttctgg agaaaagac tgactcagg aaatcaaaa 
      aaacggacg ttgttgttg aaaaaagtg accaaaacg 
      aaccgttaa acttgtgca ttcgtgact taaaccact 
      tgaaaatcc gtcttttga agaagaacg aactaaagt 
      cggagccag ggagtgacg acgacgtgg agttcgtgc 
      acgaaaccc catcccctg ggaagcccg aacccttgg 
      ggacgccgc ccgaagccc caggtggtt acgtctact 
      ggcccaatg ccggggtgt tcatccacc cgatggccc 
      tccagcagc agctaatgt actaccaga ggatggcgg 
      ccaacaaca gctacaccc tccccagcc acagcccgc 
      ggcccccgc cccccgacc ccgagcccg aactcagga 
      aaatcaccc cgaaagcgc gcaaaaaac ccgaagaga 
      ctgagcccc aacctgagc aaaagacca aacgcaacg 
      cctccacct cctcggaca agaccccca ccaagcgga 
      aacgctccg ccatcttca ttcctccca tcccccccg 
      aaaacaaca caaactcag ccaccgaag tcagtattt 
      gcaagttta aattcaccg gaggtgcga aacctagtc 
      tacaagaaa aaaaaatgc tgtcggtcg attccggcg 
      gcaattttc gctactaca gcggaacgg gggacaagt 
      caatgcgcg gctacgagt tcttcccgc gggaaaccc 
      tccagcagc agatcaaca actcgcaag gctccagca 
      ccggagcgt tcctgcaag cgagtgggg agaagattt 
      atccggtgg ctccggtcg atttcaccg gaactaagc 
      cgagtcagg agtttctgc tggctccgg aagcgcccc 
      caacgcctg acttgcacc ggcataaga aacgcaagt 
      ccaggaagt cgctccaga gggagaagc tggagcaga 
      cgttcaagg agaaggggt ttctgatac agacgcagc 
      agctggaat cggctgaag gcgctactt attgcaagt 
      ttagacagt tgaggaagt ttacgaggt atttgttca 
      ggagttgga aggattatc tgccgggga atgtgcgcg 
      agttgcaag agggggaaa aaccggatg aggaagatg 
      gtgataatt tatccagac attcgagtg agttgcagt 
      gaaacgacc aaagattgc gctgttttg ggacgctga 
      gtggtgcta attattgat aatctagtc gtaattcat 
      taatgttgt taatcgtaa gggaattgt taataaaac 
      tcgctgttt cac