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Gene information
General information
TC007666 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1139026..1144713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007666
    iBeetle screen iB_01239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007666 RA
      MLYFDEGHN IEKICEDSA SIQIKSADI ALCIDEVTG 
      VMRALSDET ILDFNDKEV PKDFTPDEL CILKEIFLN 
      FEKVLDEIE VKAGAPEGT YFEGSYIFE MFNKAGIND 
      GNYNSVLSV MDKIIQFLA SGNEGPFAR KGVGLQLFS 
      DLLTIAFSS VSPQFKEKI KRCYKVHIA VEEGKKKAA 
      DDWLSKATL KTRSRVLSY WCFSPGFGM NMLLDQGVH 
      CVILTSGTL APLKPLISE LELNVGVRI ENPHIVKGD 
      QVCVKILTK GPDGEPLNC NYQNRDNPN YLMSLGQVV 
      LNLIRIIPH GVLIFFPSY PIMQKCQQY WQESGIWDG 
      INKIKAIFV EPKDKNSFT FAMSEYYSK VKDPNYKGA 
      IFMGVCRGK VSEGLDFAD SNGRAVFII GLPYPPLKD 
      PKIILKKRY LDVCNAKDK EYLKGDDWY SLEATRAIN 
      QAIGRVIRH KNDYGAIIL LDSRFTNPR IQGNLSLWL 
      KKHIKIMQN FGALTRDLR LFFQNAQSK LPAPKLRDE 
      NITPNEGVT TRVGNFNFS SSSGSLTPA GSSGLVNIH 
      KRKTTESEN PVKKMKITV IANRKSDKP VEASISEYI 
      IMVKKTLSP EYFKQFINL LAIYRANCD FNGLQTGLD 
      EIFATRHHL RYIIRGLEP YIKDEHKSA FNVYCDAKV 
      LLRLPNLTF PTKPPLIKV AFSCIQPDF FTKMTTITI 
      RGVPVKFPF APYDIQTKY MEKVIDCLE NRQNGILES 
      PTGTGKTLS LLCASLAWL EARREKFAA QPKKCDDSP 
      PITLPRIIY ASRTHTQLS QAMQEMKRT AYNHLKACV 
      LGSRDQMCI DPEVIQEKN ASFKVNLCR AKVKRKQCK 
      YYQRIERAS HVPPVSDLN IIDIEDVVK LGRECDFCP 
      YHMARELKN RSDVVFMPY NYLLDPRTS KNMDVEIFG 
      NIIIFDEAH NIEKICEDS VSVQIKSSD VDSAVEDVE 
      SVNEVLTKG FVDVENVAF TSEDLQTFS QMLTKFNRE 
      LNGIKQFKL TQAGSVFDG DYIFEMFKK AEIFEENSS 
      GISDLIVGV LDFMSTTSY IQRSGFGLQ IFNNLLFIA 
      FYKQGPEFR QKIKECFKV QVEDERPEY VRRTRYSQN 
      MDKKNTCRV LNFWCFSPG FAMNMLMDR NIHCVILTS 
      GTLAPLKPL ISELELEIG VRIENPHIV KGDQVCVKI 
      LSKGPDMEP LNSNFENRS NPKYLQSLG LVISNLIRV 
      IPDGVLIFF PSYVIMQKT IEHWQNDGT WDSINRTKP 
      IYIEPKDKI EFATAMSEY YAKIQDPSY SGAIFMGVC 
      RGKVSEGLD FADINGRAV FITGLPYPP LKDPKIILK 
      KQYLDFRYS KNKEYLKGD DWYSLEATR AINQAIGRV 
      IRHKSDYGA IILLDSRFT NPRIKNNLS LWLRQHITD 
      MKNFGEIIR NVRVFFQNA QKFCTPYVK EIAIEPLIE 
      ASTSKEINS REEEPWDCS PPKKNR
    CDS sequence
    • >TC007666 RA
      atgttatat tttgacgag ggccacaat attgagaaa 
      atctgtgaa gactcggcc agtattcag ataaaatcg 
      gctgatatc gcgctttgt attgacgag gtcactggt 
      gttatgagg gccttaagt gacgaaacg atactcgat 
      tttaacgat aaagaagtc ccgaaagac ttcacccct 
      gatgagctt tgcatactt aaagaaatt ttcctaaac 
      tttgaaaaa gtcctcgat gagattgag gtcaaggcg 
      ggggcgccc gaaggcacg tactttgag ggcagctat 
      atttttgaa atgtttaac aaggctggg attaacgat 
      ggcaattac aattcggtc ctttctgtg atggacaaa 
      ataatacag tttttggcg tctggaaac gaggggccc 
      tttgcccgg aaaggggtc ggcttgcag ttattttcc 
      gatttgtta acaatcgca ttttcaagc gtcagcccc 
      cagtttaag gaaaaaata aaaaggtgt tacaaggtc 
      cacattgcc gttgaagag ggtaagaaa aaagcggca 
      gatgactgg ttgtcgaaa gcaactctc aaaactcgc 
      tcgcgtgtt ctcagctat tggtgcttc agtcccggt 
      tttgggatg aacatgttg ctggaccaa ggtgtccac 
      tgtgtcatt ctaacaagt ggcactttg gccccctta 
      aagccccta atttcggaa ctagagcta aacgtcggc 
      gtccgtatt gaaaacccc cacattgtt aaaggcgac 
      caagtttgt gttaaaatt ctaactaaa ggacccgat 
      ggcgaaccc ctcaactgt aattaccaa aaccgcgac 
      aatccgaat tatttaatg tcgctagga caagtcgta 
      ttgaattta atccgaatt attccacac ggagttcta 
      atttttttc ccgtcttat ccgattatg caaaagtgc 
      caacaatac tggcaggag tcgggtatt tgggacgga 
      ataaataaa atcaaagcg atttttgtc gaacctaaa 
      gacaaaaac tcgttcact ttcgccatg tctgagtac 
      tattcgaaa gtcaaagac ccgaattac aagggggct 
      atttttatg ggggtgtgt cggggcaaa gtctccgaa 
      gggttagat ttcgctgat agtaacggc cgggctgtt 
      tttatcata ggcttaccg tacccgcct ctcaaggac 
      cccaaaatt atcctgaaa aaacggtac ttggacgtg 
      tgcaatgcc aaggacaag gagtatctg aagggtgac 
      gactggtac agcttggaa gccacccgt gccataaac 
      caggccata ggccgagtc attcgccat aaaaacgat 
      tacggcgcc ataattctc ctcgattcg cgtttcaca 
      aaccctcga atccaaggc aatctctca ctatggctc 
      aaaaaacac atcaaaatt atgcaaaat ttcggagcc 
      ttgacgcgt gatctccga ttatttttc caaaatgct 
      caatcaaag ttgcctgcg cccaaactc agggatgaa 
      aatattacg ccaaatgaa ggggtgaca acaagggtt 
      ggtaatttt aattttagt tcgagttca ggttcatta 
      actcccgct ggaagtagc ggattggtg aatattcat 
      aaacgaaaa acaacggag agtgaaaat ccagttaaa 
      aaaatgaaa attactgtg atagctaac cggaaaagt 
      gataagcca gtggaggct tcaatttcg gaatatatt 
      attatggta aaaaagact ctaagtccg gaatatttt 
      aagcaattt attaattta ctggcgatt tatcgagct 
      aattgcgat ttcaacgga cttcaaaca ggtcttgat 
      gagattttt gctacgcgc catcattta agatatatc 
      atcaggggg ttggagccc tacattaag gatgaacac 
      aagagtgca tttaatgta tattgtgac gccaaggtg 
      cttttacga ctccctaac ctgactttt cccacaaaa 
      ccaccgttg ataaaagtg gctttttca tgtatacaa 
      cccgacttt tttacaaaa atgacaaca ataacaatc 
      cggggcgtc cccgtgaag ttcccgttc gccccctac 
      gacatccag accaaatac atggaaaaa gtaatcgac 
      tgtttggag aaccgccaa aacggcatt ctagaatcg 
      cccacaggc acaggcaag actctttcc ctcctgtgt 
      gcctcccta gcctggctc gaagcccgg cgagaaaaa 
      tttgctgca cagccaaaa aaatgtgac gacagcccc 
      cctataacc ctccccagg atcatctac gcgtcccga 
      acgcacaca caattgagt caagccatg caggagatg 
      aaacgaacg gcttacaat cacctcaaa gcgtgcgtt 
      ttggggtcc cgggaccaa atgtgcata gaccccgaa 
      gttattcag gaaaaaaat gcctcgttt aaagtgaat 
      ctgtgtcgc gcgaaagtg aaacgaaaa cagtgtaaa 
      tattaccaa aggattgaa cgggcgagt catgtaccc 
      ccagttagc gacttaaat atcattgat atcgaagat 
      gtggttaaa ttggggcgt gagtgtgat ttttgtccc 
      taccacatg gcccgagaa ttgaaaaat cggtcagat 
      gtggtcttc atgccctat aattacttg ctagaccct 
      cggactagt aaaaatatg gacgttgag atttttgga 
      aatattatt atttttgac gaggcacac aatattgaa 
      aaaatttgc gaagactca gtttcggta caaatcaaa 
      tcgagtgat gttgattca gctgttgag gatgttgag 
      tctgttaat gaagtttta acgaaggga tttgtcgac 
      gttgaaaat gttgcgttc acttcggaa gatttacaa 
      actttcagc caaatgttg accaagttt aaccgggaa 
      ttaaacggc ataaaacaa ttcaaatta acacaagct 
      gggagcgtt tttgacggt gattatatc ttcgaaatg 
      ttcaagaaa gctgagata tttgaagaa aacagttcg 
      gggataagt gacttgatt gtgggtgtt ttagatttt 
      atgtccacc acaagctac atacagaga agcgggttt 
      gggttacaa attttcaac aatttgctg tttattgca 
      ttttacaaa cagggacca gaatttcgg caaaaaatt 
      aaagaatgt ttcaaagta caagtggaa gacgaaaga 
      cccgaatat gtcaggaga actcggtac tcgcaaaat 
      atggacaaa aaaaacact tgtcgcgtg ttaaatttt 
      tggtgtttc agtcccggg tttgcaatg aatatgtta 
      atggaccgc aacatccac tgcgtaatc ctcactagc 
      ggcaccttg gccccctta aaacccctg atatcggaa 
      ctggaattg gaaattggg gtccgaatt gaaaacccc 
      cacattgtg aaaggcgac caagtttgt gtcaaaatt 
      ttgagcaaa ggccccgat atggagccc ctaaatagc 
      aacttcgaa aatcgctct aatcccaaa tatttacaa 
      tcattgggg ctcgtaatt tcaaatcta atccgggta 
      atccccgac ggtgtttta atttttttc ccttcttat 
      gtcattatg caaaaaacg atcgaacac tggcaaaac 
      gacggcact tgggattcg ataaaccgg actaaaccg 
      atttatatc gaacctaaa gacaaaatt gaatttgcg 
      accgccatg tctgagtat tatgcaaaa attcaagac 
      ccgagctac agtggggct atttttatg ggggtgtgt 
      cggggcaaa gtctcggaa ggtttagat tttgccgat 
      atcaacggc agggctgtc tttataacc gggctcccg 
      tatcccccc ttaaaagac cccaaaata atactcaag 
      aaacagtac ctggacttc cgctactca aaaaataaa 
      gagtacctg aaaggcgac gattggtac agtttggaa 
      gccacccgt gccataaat caggccata ggccgagta 
      atccgccac aaaagtgac tatggggca attattttg 
      ctcgattct cgcttcacc aatccgcga ataaaaaac 
      aacttgtct ttatggttg cgccaacac atcaccgat 
      atgaaaaac ttcggcgaa attatacgc aacgttcgt 
      gtgtttttt caaaatgct caaaaattt tgcacgccc 
      tacgttaag gaaatcgcc attgagcca cttattgag 
      gcatcaaca agcaaggaa ataaattca cgggaagag 
      gaaccttgg gactgttcg cctccaaag aaaaaccgc 
      tag
    mRNA sequence
    • >TC007666 RA
      tagaatagc caatatgtt atattttga cgagggcca 
      caatattga gaaaatctg tgaagactc ggccagtat 
      tcagataaa atcggctga tatcgcgct ttgtattga 
      cgaggtcac tggtgttat gagggcctt aagtgacga 
      aacgatact cgattttaa cgataaaga agtcccgaa 
      agacttcac ccctgatga gctttgcat acttaaaga 
      aattttcct aaactttga aaaagtcct cgatgagat 
      tgaggtcaa ggcgggggc gcccgaagg cacgtactt 
      tgagggcag ctatatttt tgaaatgtt taacaaggc 
      tgggattaa cgatggcaa ttacaattc ggtcctttc 
      tgtgatgga caaaataat acagttttt ggcgtctgg 
      aaacgaggg gccctttgc ccggaaagg ggtcggctt 
      gcagttatt ttccgattt gttaacaat cgcattttc 
      aagcgtcag cccccagtt taaggaaaa aataaaaag 
      gtgttacaa ggtccacat tgccgttga agagggtaa 
      gaaaaaagc ggcagatga ctggttgtc gaaagcaac 
      tctcaaaac tcgctcgcg tgttctcag ctattggtg 
      cttcagtcc cggttttgg gatgaacat gttgctgga 
      ccaaggtgt ccactgtgt cattctaac aagtggcac 
      tttggcccc cttaaagcc cctaatttc ggaactaga 
      gctaaacgt cggcgtccg tattgaaaa cccccacat 
      tgttaaagg cgaccaagt ttgtgttaa aattctaac 
      taaaggacc cgatggcga acccctcaa ctgtaatta 
      ccaaaaccg cgacaatcc gaattattt aatgtcgct 
      aggacaagt cgtattgaa tttaatccg aattattcc 
      acacggagt tctaatttt tttcccgtc ttatccgat 
      tatgcaaaa gtgccaaca atactggca ggagtcggg 
      tatttggga cggaataaa taaaatcaa agcgatttt 
      tgtcgaacc taaagacaa aaactcgtt cactttcgc 
      catgtctga gtactattc gaaagtcaa agacccgaa 
      ttacaaggg ggctatttt tatgggggt gtgtcgggg 
      caaagtctc cgaagggtt agatttcgc tgatagtaa 
      cggccgggc tgtttttat cataggctt accgtaccc 
      gcctctcaa ggaccccaa aattatcct gaaaaaacg 
      gtacttgga cgtgtgcaa tgccaagga caaggagta 
      tctgaaggg tgacgactg gtacagctt ggaagccac 
      ccgtgccat aaaccaggc cataggccg agtcattcg 
      ccataaaaa cgattacgg cgccataat tctcctcga 
      ttcgcgttt cacaaaccc tcgaatcca aggcaatct 
      ctcactatg gctcaaaaa acacatcaa aattatgca 
      aaatttcgg agccttgac gcgtgatct ccgattatt 
      tttccaaaa tgctcaatc aaagttgcc tgcgcccaa 
      actcaggga tgaaaatat tacgccaaa tgaaggggt 
      gacaacaag ggttggtaa ttttaattt tagttcgag 
      ttcaggttc attaactcc cgctggaag tagcggatt 
      ggtgaatat tcataaacg aaaaacaac ggagagtga 
      aaatccagt taaaaaaat gaaaattac tgtgatagc 
      taaccggaa aagtgataa gccagtgga ggcttcaat 
      ttcggaata tattattat ggtaaaaaa gactctaag 
      tccggaata ttttaagca atttattaa tttactggc 
      gatttatcg agctaattg cgatttcaa cggacttca 
      aacaggtct tgatgagat ttttgctac gcgccatca 
      tttaagata tatcatcag ggggttgga gccctacat 
      taaggatga acacaagag tgcatttaa tgtatattg 
      tgacgccaa ggtgctttt acgactccc taacctgac 
      ttttcccac aaaaccacc gttgataaa agtggcttt 
      ttcatgtat acaacccga cttttttac aaaaatgac 
      aacaataac aatccgggg cgtccccgt gaagttccc 
      gttcgcccc ctacgacat ccagaccaa atacatgga 
      aaaagtaat cgactgttt ggagaaccg ccaaaacgg 
      cattctaga atcgcccac aggcacagg caagactct 
      ttccctcct gtgtgcctc cctagcctg gctcgaagc 
      ccggcgaga aaaatttgc tgcacagcc aaaaaaatg 
      tgacgacag cccccctat aaccctccc caggatcat 
      ctacgcgtc ccgaacgca cacacaatt gagtcaagc 
      catgcagga gatgaaacg aacggctta caatcacct 
      caaagcgtg cgttttggg gtcccggga ccaaatgtg 
      catagaccc cgaagttat tcaggaaaa aaatgcctc 
      gtttaaagt gaatctgtg tcgcgcgaa agtgaaacg 
      aaaacagtg taaatatta ccaaaggat tgaacgggc 
      gagtcatgt acccccagt tagcgactt aaatatcat 
      tgatatcga agatgtggt taaattggg gcgtgagtg 
      tgatttttg tccctacca catggcccg agaattgaa 
      aaatcggtc agatgtggt cttcatgcc ctataatta 
      cttgctaga ccctcggac tagtaaaaa tatggacgt 
      tgagatttt tggaaatat tattatttt tgacgaggc 
      acacaatat tgaaaaaat ttgcgaaga ctcagtttc 
      ggtacaaat caaatcgag tgatgttga ttcagctgt 
      tgaggatgt tgagtctgt taatgaagt tttaacgaa 
      gggatttgt cgacgttga aaatgttgc gttcacttc 
      ggaagattt acaaacttt cagccaaat gttgaccaa 
      gtttaaccg ggaattaaa cggcataaa acaattcaa 
      attaacaca agctgggag cgtttttga cggtgatta 
      tatcttcga aatgttcaa gaaagctga gatatttga 
      agaaaacag ttcggggat aagtgactt gattgtggg 
      tgttttaga ttttatgtc caccacaag ctacataca 
      gagaagcgg gtttgggtt acaaatttt caacaattt 
      gctgtttat tgcatttta caaacaggg accagaatt 
      tcggcaaaa aattaaaga atgtttcaa agtacaagt 
      ggaagacga aagacccga atatgtcag gagaactcg 
      gtactcgca aaatatgga caaaaaaaa cacttgtcg 
      cgtgttaaa tttttggtg tttcagtcc cgggtttgc 
      aatgaatat gttaatgga ccgcaacat ccactgcgt 
      aatcctcac tagcggcac cttggcccc cttaaaacc 
      cctgatatc ggaactgga attggaaat tggggtccg 
      aattgaaaa cccccacat tgtgaaagg cgaccaagt 
      ttgtgtcaa aattttgag caaaggccc cgatatgga 
      gcccctaaa tagcaactt cgaaaatcg ctctaatcc 
      caaatattt acaatcatt ggggctcgt aatttcaaa 
      tctaatccg ggtaatccc cgacggtgt tttaatttt 
      tttcccttc ttatgtcat tatgcaaaa aacgatcga 
      acactggca aaacgacgg cacttggga ttcgataaa 
      ccggactaa accgattta tatcgaacc taaagacaa 
      aattgaatt tgcgaccgc catgtctga gtattatgc 
      aaaaattca agacccgag ctacagtgg ggctatttt 
      tatgggggt gtgtcgggg caaagtctc ggaaggttt 
      agattttgc cgatatcaa cggcagggc tgtctttat 
      aaccgggct cccgtatcc ccccttaaa agaccccaa 
      aataatact caagaaaca gtacctgga cttccgcta 
      ctcaaaaaa taaagagta cctgaaagg cgacgattg 
      gtacagttt ggaagccac ccgtgccat aaatcaggc 
      cataggccg agtaatccg ccacaaaag tgactatgg 
      ggcaattat tttgctcga ttctcgctt caccaatcc 
      gcgaataaa aaacaactt gtctttatg gttgcgcca 
      acacatcac cgatatgaa aaacttcgg cgaaattat 
      acgcaacgt tcgtgtgtt ttttcaaaa tgctcaaaa 
      attttgcac gccctacgt taaggaaat cgccattga 
      gccacttat tgaggcatc aacaagcaa ggaaataaa 
      ttcacggga agaggaacc ttgggactg ttcgcctcc 
      aaagaaaaa ccgctagtg ttattactt gtagtttta 
      atacgaa
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