Status
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Gene information
General information
TC007666 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1139026..1144713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007666
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007666 RA
      MLYFDEGHN IEKICEDSA SIQIKSADI ALCIDEVTG 
VMRALSDET ILDFNDKEV PKDFTPDEL CILKEIFLN 
FEKVLDEIE VKAGAPEGT YFEGSYIFE MFNKAGIND 
GNYNSVLSV MDKIIQFLA SGNEGPFAR KGVGLQLFS 
DLLTIAFSS VSPQFKEKI KRCYKVHIA VEEGKKKAA 
DDWLSKATL KTRSRVLSY WCFSPGFGM NMLLDQGVH 
CVILTSGTL APLKPLISE LELNVGVRI ENPHIVKGD 
QVCVKILTK GPDGEPLNC NYQNRDNPN YLMSLGQVV 
LNLIRIIPH GVLIFFPSY PIMQKCQQY WQESGIWDG 
INKIKAIFV EPKDKNSFT FAMSEYYSK VKDPNYKGA 
IFMGVCRGK VSEGLDFAD SNGRAVFII GLPYPPLKD 
PKIILKKRY LDVCNAKDK EYLKGDDWY SLEATRAIN 
QAIGRVIRH KNDYGAIIL LDSRFTNPR IQGNLSLWL 
KKHIKIMQN FGALTRDLR LFFQNAQSK LPAPKLRDE 
NITPNEGVT TRVGNFNFS SSSGSLTPA GSSGLVNIH 
KRKTTESEN PVKKMKITV IANRKSDKP VEASISEYI 
IMVKKTLSP EYFKQFINL LAIYRANCD FNGLQTGLD 
EIFATRHHL RYIIRGLEP YIKDEHKSA FNVYCDAKV 
LLRLPNLTF PTKPPLIKV AFSCIQPDF FTKMTTITI 
RGVPVKFPF APYDIQTKY MEKVIDCLE NRQNGILES 
PTGTGKTLS LLCASLAWL EARREKFAA QPKKCDDSP 
PITLPRIIY ASRTHTQLS QAMQEMKRT AYNHLKACV 
LGSRDQMCI DPEVIQEKN ASFKVNLCR AKVKRKQCK 
YYQRIERAS HVPPVSDLN IIDIEDVVK LGRECDFCP 
YHMARELKN RSDVVFMPY NYLLDPRTS KNMDVEIFG 
NIIIFDEAH NIEKICEDS VSVQIKSSD VDSAVEDVE 
SVNEVLTKG FVDVENVAF TSEDLQTFS QMLTKFNRE 
LNGIKQFKL TQAGSVFDG DYIFEMFKK AEIFEENSS 
GISDLIVGV LDFMSTTSY IQRSGFGLQ IFNNLLFIA 
FYKQGPEFR QKIKECFKV QVEDERPEY VRRTRYSQN 
MDKKNTCRV LNFWCFSPG FAMNMLMDR NIHCVILTS 
GTLAPLKPL ISELELEIG VRIENPHIV KGDQVCVKI 
LSKGPDMEP LNSNFENRS NPKYLQSLG LVISNLIRV 
IPDGVLIFF PSYVIMQKT IEHWQNDGT WDSINRTKP 
IYIEPKDKI EFATAMSEY YAKIQDPSY SGAIFMGVC 
RGKVSEGLD FADINGRAV FITGLPYPP LKDPKIILK 
KQYLDFRYS KNKEYLKGD DWYSLEATR AINQAIGRV 
IRHKSDYGA IILLDSRFT NPRIKNNLS LWLRQHITD 
MKNFGEIIR NVRVFFQNA QKFCTPYVK EIAIEPLIE 
ASTSKEINS REEEPWDCS PPKKNR
    CDS sequence
    • >TC007666 RA
      atgttatat tttgacgag ggccacaat attgagaaa 
atctgtgaa gactcggcc agtattcag ataaaatcg 
gctgatatc gcgctttgt attgacgag gtcactggt 
gttatgagg gccttaagt gacgaaacg atactcgat 
tttaacgat aaagaagtc ccgaaagac ttcacccct 
gatgagctt tgcatactt aaagaaatt ttcctaaac 
tttgaaaaa gtcctcgat gagattgag gtcaaggcg 
ggggcgccc gaaggcacg tactttgag ggcagctat 
atttttgaa atgtttaac aaggctggg attaacgat 
ggcaattac aattcggtc ctttctgtg atggacaaa 
ataatacag tttttggcg tctggaaac gaggggccc 
tttgcccgg aaaggggtc ggcttgcag ttattttcc 
gatttgtta acaatcgca ttttcaagc gtcagcccc 
cagtttaag gaaaaaata aaaaggtgt tacaaggtc 
cacattgcc gttgaagag ggtaagaaa aaagcggca 
gatgactgg ttgtcgaaa gcaactctc aaaactcgc 
tcgcgtgtt ctcagctat tggtgcttc agtcccggt 
tttgggatg aacatgttg ctggaccaa ggtgtccac 
tgtgtcatt ctaacaagt ggcactttg gccccctta 
aagccccta atttcggaa ctagagcta aacgtcggc 
gtccgtatt gaaaacccc cacattgtt aaaggcgac 
caagtttgt gttaaaatt ctaactaaa ggacccgat 
ggcgaaccc ctcaactgt aattaccaa aaccgcgac 
aatccgaat tatttaatg tcgctagga caagtcgta 
ttgaattta atccgaatt attccacac ggagttcta 
atttttttc ccgtcttat ccgattatg caaaagtgc 
caacaatac tggcaggag tcgggtatt tgggacgga 
ataaataaa atcaaagcg atttttgtc gaacctaaa 
gacaaaaac tcgttcact ttcgccatg tctgagtac 
tattcgaaa gtcaaagac ccgaattac aagggggct 
atttttatg ggggtgtgt cggggcaaa gtctccgaa 
gggttagat ttcgctgat agtaacggc cgggctgtt 
tttatcata ggcttaccg tacccgcct ctcaaggac 
cccaaaatt atcctgaaa aaacggtac ttggacgtg 
tgcaatgcc aaggacaag gagtatctg aagggtgac 
gactggtac agcttggaa gccacccgt gccataaac 
caggccata ggccgagtc attcgccat aaaaacgat 
tacggcgcc ataattctc ctcgattcg cgtttcaca 
aaccctcga atccaaggc aatctctca ctatggctc 
aaaaaacac atcaaaatt atgcaaaat ttcggagcc 
ttgacgcgt gatctccga ttatttttc caaaatgct 
caatcaaag ttgcctgcg cccaaactc agggatgaa 
aatattacg ccaaatgaa ggggtgaca acaagggtt 
ggtaatttt aattttagt tcgagttca ggttcatta 
actcccgct ggaagtagc ggattggtg aatattcat 
aaacgaaaa acaacggag agtgaaaat ccagttaaa 
aaaatgaaa attactgtg atagctaac cggaaaagt 
gataagcca gtggaggct tcaatttcg gaatatatt 
attatggta aaaaagact ctaagtccg gaatatttt 
aagcaattt attaattta ctggcgatt tatcgagct 
aattgcgat ttcaacgga cttcaaaca ggtcttgat 
gagattttt gctacgcgc catcattta agatatatc 
atcaggggg ttggagccc tacattaag gatgaacac 
aagagtgca tttaatgta tattgtgac gccaaggtg 
cttttacga ctccctaac ctgactttt cccacaaaa 
ccaccgttg ataaaagtg gctttttca tgtatacaa 
cccgacttt tttacaaaa atgacaaca ataacaatc 
cggggcgtc cccgtgaag ttcccgttc gccccctac 
gacatccag accaaatac atggaaaaa gtaatcgac 
tgtttggag aaccgccaa aacggcatt ctagaatcg 
cccacaggc acaggcaag actctttcc ctcctgtgt 
gcctcccta gcctggctc gaagcccgg cgagaaaaa 
tttgctgca cagccaaaa aaatgtgac gacagcccc 
cctataacc ctccccagg atcatctac gcgtcccga 
acgcacaca caattgagt caagccatg caggagatg 
aaacgaacg gcttacaat cacctcaaa gcgtgcgtt 
ttggggtcc cgggaccaa atgtgcata gaccccgaa 
gttattcag gaaaaaaat gcctcgttt aaagtgaat 
ctgtgtcgc gcgaaagtg aaacgaaaa cagtgtaaa 
tattaccaa aggattgaa cgggcgagt catgtaccc 
ccagttagc gacttaaat atcattgat atcgaagat 
gtggttaaa ttggggcgt gagtgtgat ttttgtccc 
taccacatg gcccgagaa ttgaaaaat cggtcagat 
gtggtcttc atgccctat aattacttg ctagaccct 
cggactagt aaaaatatg gacgttgag atttttgga 
aatattatt atttttgac gaggcacac aatattgaa 
aaaatttgc gaagactca gtttcggta caaatcaaa 
tcgagtgat gttgattca gctgttgag gatgttgag 
tctgttaat gaagtttta acgaaggga tttgtcgac 
gttgaaaat gttgcgttc acttcggaa gatttacaa 
actttcagc caaatgttg accaagttt aaccgggaa 
ttaaacggc ataaaacaa ttcaaatta acacaagct 
gggagcgtt tttgacggt gattatatc ttcgaaatg 
ttcaagaaa gctgagata tttgaagaa aacagttcg 
gggataagt gacttgatt gtgggtgtt ttagatttt 
atgtccacc acaagctac atacagaga agcgggttt 
gggttacaa attttcaac aatttgctg tttattgca 
ttttacaaa cagggacca gaatttcgg caaaaaatt 
aaagaatgt ttcaaagta caagtggaa gacgaaaga 
cccgaatat gtcaggaga actcggtac tcgcaaaat 
atggacaaa aaaaacact tgtcgcgtg ttaaatttt 
tggtgtttc agtcccggg tttgcaatg aatatgtta 
atggaccgc aacatccac tgcgtaatc ctcactagc 
ggcaccttg gccccctta aaacccctg atatcggaa 
ctggaattg gaaattggg gtccgaatt gaaaacccc 
cacattgtg aaaggcgac caagtttgt gtcaaaatt 
ttgagcaaa ggccccgat atggagccc ctaaatagc 
aacttcgaa aatcgctct aatcccaaa tatttacaa 
tcattgggg ctcgtaatt tcaaatcta atccgggta 
atccccgac ggtgtttta atttttttc ccttcttat 
gtcattatg caaaaaacg atcgaacac tggcaaaac 
gacggcact tgggattcg ataaaccgg actaaaccg 
atttatatc gaacctaaa gacaaaatt gaatttgcg 
accgccatg tctgagtat tatgcaaaa attcaagac 
ccgagctac agtggggct atttttatg ggggtgtgt 
cggggcaaa gtctcggaa ggtttagat tttgccgat 
atcaacggc agggctgtc tttataacc gggctcccg 
tatcccccc ttaaaagac cccaaaata atactcaag 
aaacagtac ctggacttc cgctactca aaaaataaa 
gagtacctg aaaggcgac gattggtac agtttggaa 
gccacccgt gccataaat caggccata ggccgagta 
atccgccac aaaagtgac tatggggca attattttg 
ctcgattct cgcttcacc aatccgcga ataaaaaac 
aacttgtct ttatggttg cgccaacac atcaccgat 
atgaaaaac ttcggcgaa attatacgc aacgttcgt 
gtgtttttt caaaatgct caaaaattt tgcacgccc 
tacgttaag gaaatcgcc attgagcca cttattgag 
gcatcaaca agcaaggaa ataaattca cgggaagag 
gaaccttgg gactgttcg cctccaaag aaaaaccgc 
tag
    mRNA sequence
    • >TC007666 RA
      tagaatagc caatatgtt atattttga cgagggcca 
caatattga gaaaatctg tgaagactc ggccagtat 
tcagataaa atcggctga tatcgcgct ttgtattga 
cgaggtcac tggtgttat gagggcctt aagtgacga 
aacgatact cgattttaa cgataaaga agtcccgaa 
agacttcac ccctgatga gctttgcat acttaaaga 
aattttcct aaactttga aaaagtcct cgatgagat 
tgaggtcaa ggcgggggc gcccgaagg cacgtactt 
tgagggcag ctatatttt tgaaatgtt taacaaggc 
tgggattaa cgatggcaa ttacaattc ggtcctttc 
tgtgatgga caaaataat acagttttt ggcgtctgg 
aaacgaggg gccctttgc ccggaaagg ggtcggctt 
gcagttatt ttccgattt gttaacaat cgcattttc 
aagcgtcag cccccagtt taaggaaaa aataaaaag 
gtgttacaa ggtccacat tgccgttga agagggtaa 
gaaaaaagc ggcagatga ctggttgtc gaaagcaac 
tctcaaaac tcgctcgcg tgttctcag ctattggtg 
cttcagtcc cggttttgg gatgaacat gttgctgga 
ccaaggtgt ccactgtgt cattctaac aagtggcac 
tttggcccc cttaaagcc cctaatttc ggaactaga 
gctaaacgt cggcgtccg tattgaaaa cccccacat 
tgttaaagg cgaccaagt ttgtgttaa aattctaac 
taaaggacc cgatggcga acccctcaa ctgtaatta 
ccaaaaccg cgacaatcc gaattattt aatgtcgct 
aggacaagt cgtattgaa tttaatccg aattattcc 
acacggagt tctaatttt tttcccgtc ttatccgat 
tatgcaaaa gtgccaaca atactggca ggagtcggg 
tatttggga cggaataaa taaaatcaa agcgatttt 
tgtcgaacc taaagacaa aaactcgtt cactttcgc 
catgtctga gtactattc gaaagtcaa agacccgaa 
ttacaaggg ggctatttt tatgggggt gtgtcgggg 
caaagtctc cgaagggtt agatttcgc tgatagtaa 
cggccgggc tgtttttat cataggctt accgtaccc 
gcctctcaa ggaccccaa aattatcct gaaaaaacg 
gtacttgga cgtgtgcaa tgccaagga caaggagta 
tctgaaggg tgacgactg gtacagctt ggaagccac 
ccgtgccat aaaccaggc cataggccg agtcattcg 
ccataaaaa cgattacgg cgccataat tctcctcga 
ttcgcgttt cacaaaccc tcgaatcca aggcaatct 
ctcactatg gctcaaaaa acacatcaa aattatgca 
aaatttcgg agccttgac gcgtgatct ccgattatt 
tttccaaaa tgctcaatc aaagttgcc tgcgcccaa 
actcaggga tgaaaatat tacgccaaa tgaaggggt 
gacaacaag ggttggtaa ttttaattt tagttcgag 
ttcaggttc attaactcc cgctggaag tagcggatt 
ggtgaatat tcataaacg aaaaacaac ggagagtga 
aaatccagt taaaaaaat gaaaattac tgtgatagc 
taaccggaa aagtgataa gccagtgga ggcttcaat 
ttcggaata tattattat ggtaaaaaa gactctaag 
tccggaata ttttaagca atttattaa tttactggc 
gatttatcg agctaattg cgatttcaa cggacttca 
aacaggtct tgatgagat ttttgctac gcgccatca 
tttaagata tatcatcag ggggttgga gccctacat 
taaggatga acacaagag tgcatttaa tgtatattg 
tgacgccaa ggtgctttt acgactccc taacctgac 
ttttcccac aaaaccacc gttgataaa agtggcttt 
ttcatgtat acaacccga cttttttac aaaaatgac 
aacaataac aatccgggg cgtccccgt gaagttccc 
gttcgcccc ctacgacat ccagaccaa atacatgga 
aaaagtaat cgactgttt ggagaaccg ccaaaacgg 
cattctaga atcgcccac aggcacagg caagactct 
ttccctcct gtgtgcctc cctagcctg gctcgaagc 
ccggcgaga aaaatttgc tgcacagcc aaaaaaatg 
tgacgacag cccccctat aaccctccc caggatcat 
ctacgcgtc ccgaacgca cacacaatt gagtcaagc 
catgcagga gatgaaacg aacggctta caatcacct 
caaagcgtg cgttttggg gtcccggga ccaaatgtg 
catagaccc cgaagttat tcaggaaaa aaatgcctc 
gtttaaagt gaatctgtg tcgcgcgaa agtgaaacg 
aaaacagtg taaatatta ccaaaggat tgaacgggc 
gagtcatgt acccccagt tagcgactt aaatatcat 
tgatatcga agatgtggt taaattggg gcgtgagtg 
tgatttttg tccctacca catggcccg agaattgaa 
aaatcggtc agatgtggt cttcatgcc ctataatta 
cttgctaga ccctcggac tagtaaaaa tatggacgt 
tgagatttt tggaaatat tattatttt tgacgaggc 
acacaatat tgaaaaaat ttgcgaaga ctcagtttc 
ggtacaaat caaatcgag tgatgttga ttcagctgt 
tgaggatgt tgagtctgt taatgaagt tttaacgaa 
gggatttgt cgacgttga aaatgttgc gttcacttc 
ggaagattt acaaacttt cagccaaat gttgaccaa 
gtttaaccg ggaattaaa cggcataaa acaattcaa 
attaacaca agctgggag cgtttttga cggtgatta 
tatcttcga aatgttcaa gaaagctga gatatttga 
agaaaacag ttcggggat aagtgactt gattgtggg 
tgttttaga ttttatgtc caccacaag ctacataca 
gagaagcgg gtttgggtt acaaatttt caacaattt 
gctgtttat tgcatttta caaacaggg accagaatt 
tcggcaaaa aattaaaga atgtttcaa agtacaagt 
ggaagacga aagacccga atatgtcag gagaactcg 
gtactcgca aaatatgga caaaaaaaa cacttgtcg 
cgtgttaaa tttttggtg tttcagtcc cgggtttgc 
aatgaatat gttaatgga ccgcaacat ccactgcgt 
aatcctcac tagcggcac cttggcccc cttaaaacc 
cctgatatc ggaactgga attggaaat tggggtccg 
aattgaaaa cccccacat tgtgaaagg cgaccaagt 
ttgtgtcaa aattttgag caaaggccc cgatatgga 
gcccctaaa tagcaactt cgaaaatcg ctctaatcc 
caaatattt acaatcatt ggggctcgt aatttcaaa 
tctaatccg ggtaatccc cgacggtgt tttaatttt 
tttcccttc ttatgtcat tatgcaaaa aacgatcga 
acactggca aaacgacgg cacttggga ttcgataaa 
ccggactaa accgattta tatcgaacc taaagacaa 
aattgaatt tgcgaccgc catgtctga gtattatgc 
aaaaattca agacccgag ctacagtgg ggctatttt 
tatgggggt gtgtcgggg caaagtctc ggaaggttt 
agattttgc cgatatcaa cggcagggc tgtctttat 
aaccgggct cccgtatcc ccccttaaa agaccccaa 
aataatact caagaaaca gtacctgga cttccgcta 
ctcaaaaaa taaagagta cctgaaagg cgacgattg 
gtacagttt ggaagccac ccgtgccat aaatcaggc 
cataggccg agtaatccg ccacaaaag tgactatgg 
ggcaattat tttgctcga ttctcgctt caccaatcc 
gcgaataaa aaacaactt gtctttatg gttgcgcca 
acacatcac cgatatgaa aaacttcgg cgaaattat 
acgcaacgt tcgtgtgtt ttttcaaaa tgctcaaaa 
attttgcac gccctacgt taaggaaat cgccattga 
gccacttat tgaggcatc aacaagcaa ggaaataaa 
ttcacggga agaggaacc ttgggactg ttcgcctcc 
aaagaaaaa ccgctagtg ttattactt gtagtttta 
atacgaa
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