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Gene information
General information
TC007665 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 1146907..1148395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007665
    iBeetle screen iB_01238
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007665 RA
      MSPTLSLLT REDCEKILH RSVDSPGQL LTHKIRKFN 
      DNVAGFLGE HYQLTIKHT AKNNTTQST YFVKALPLT 
      NHNQLEYVQ ESGIFRKEV ILYQNLLTE LNKIGNFAP 
      KCYFIKDSC IVLEDMKTK GFEMLQKEK YLDETHCIA 
      MLKTLAKYH SSTIIYERK RNITLDQKF KEEIKEASF 
      SFTEGQPRQ KWCVCVTKC VNDLIKLLP IENCDTICA 
      KFERFVFEK MPQMLAPSK QFVNVLTHD DLWCNNLLF 
      NDKPECVFV DFQLVRYTP PALDFLISL FLNLPYTYA 
      MDNFTKIVD IYYKFLVEE LKKHNLDER ELSRQEFDQ 
      TLDFYKLAA LTETAMYAS HIYISEKLS NEIVADFHA 
      YNEFSFINR SKYAVKEFQ ENSHYRERV SSILKPLVE 
      LIK
    CDS sequence
    • >TC007665 RA
      atgtcgcca actctctca cttctgacc cgcgaagac 
      tgcgaaaaa atcctccac cgaagtgtc gactcccct 
      ggccaactt ctcacgcac aaaatccga aaattcaac 
      gacaatgtt gcgggattt ttgggcgaa cactaccaa 
      ctcaccatc aaacacacc gccaaaaat aacacaacc 
      cagtccact tacttcgtc aaagccctg cctttgacc 
      aaccacaac cagctggag tacgtccaa gagtcggga 
      atcttccga aaagaggtg atcttgtac cagaatctc 
      ttaaccgag ctcaacaaa attggaaat ttcgcaccc 
      aagtgctac ttcatcaag gacagttgc atcgttttg 
      gaggacatg aagaccaaa ggtttcgaa atgttgcaa 
      aaagaaaaa tatctggat gaaacacac tgtatcgca 
      atgttgaaa actttggca aaatatcac tcctcgaca 
      attatttac gaaaggaaa cgaaacatt acgctggac 
      caaaaattc aaagaggag attaaagaa gcgagcttt 
      tctttcact gagggacag ccgaggcaa aagtggtgc 
      gtttgtgtc accaaatgt gtgaacgat ttaattaaa 
      ttgctcccg atcgaaaac tgtgacacg atttgtgca 
      aaattcgag cgatttgtt ttcgaaaaa atgccacaa 
      atgctcgca ccttccaaa cagtttgtt aacgtttta 
      acacacgat gacttgtgg tgcaacaat ttgttgttc 
      aacgataag ccggagtgc gtttttgtc gacttccaa 
      cttgtgagg tacacgccc ccagccctt gatttcctc 
      atcagtttg tttctcaat ctcccatac acttatgca 
      atggataat ttcacgaaa atcgtggac atttactac 
      aaattcctc gtggaagaa ttgaagaaa cacaatttg 
      gacgaaagg gaactgtcg aggcaagag tttgaccaa 
      actttggac ttttacaaa ttagctgct ctgactgaa 
      actgccatg tatgccagt catatttac atctcggag 
      aaattatcg aacgaaatt gttgccgat tttcacgct 
      tacaacgaa ttttcgttc atcaatcgg agcaaatac 
      gcagtgaaa gaattccaa gaaaattcg cactaccgc 
      gaacgtgtt tctagcatt ttgaaacct ctggttgag 
      ttaattaaa taa
    mRNA sequence
    • >TC007665 RA
      tttctcgtc tccgaaaat gtcgccaac tctctcact 
      tctgacccg cgaagactg cgaaaaaat cctccaccg 
      aagtgtcga ctcccctgg ccaacttct cacgcacaa 
      aatccgaaa attcaacga caatgttgc gggattttt 
      gggcgaaca ctaccaact caccatcaa acacaccgc 
      caaaaataa cacaaccca gtccactta cttcgtcaa 
      agccctgcc tttgaccaa ccacaacca gctggagta 
      cgtccaaga gtcgggaat cttccgaaa agaggtgat 
      cttgtacca gaatctctt aaccgagct caacaaaat 
      tggaaattt cgcacccaa gtgctactt catcaagga 
      cagttgcat cgttttgga ggacatgaa gaccaaagg 
      tttcgaaat gttgcaaaa agaaaaata tctggatga 
      aacacactg tatcgcaat gttgaaaac tttggcaaa 
      atatcactc ctcgacaat tatttacga aaggaaacg 
      aaacattac gctggacca aaaattcaa agaggagat 
      taaagaagc gagcttttc tttcactga gggacagcc 
      gaggcaaaa gtggtgcgt ttgtgtcac caaatgtgt 
      gaacgattt aattaaatt gctcccgat cgaaaactg 
      tgacacgat ttgtgcaaa attcgagcg atttgtttt 
      cgaaaaaat gccacaaat gctcgcacc ttccaaaca 
      gtttgttaa cgttttaac acacgatga cttgtggtg 
      caacaattt gttgttcaa cgataagcc ggagtgcgt 
      ttttgtcga cttccaact tgtgaggta cacgccccc 
      agcccttga tttcctcat cagtttgtt tctcaatct 
      cccatacac ttatgcaat ggataattt cacgaaaat 
      cgtggacat ttactacaa attcctcgt ggaagaatt 
      gaagaaaca caatttgga cgaaaggga actgtcgag 
      gcaagagtt tgaccaaac tttggactt ttacaaatt 
      agctgctct gactgaaac tgccatgta tgccagtca 
      tatttacat ctcggagaa attatcgaa cgaaattgt 
      tgccgattt tcacgctta caacgaatt ttcgttcat 
      caatcggag caaatacgc agtgaaaga attccaaga 
      aaattcgca ctaccgcga acgtgtttc tagcatttt 
      gaaacctct ggttgagtt aattaaata aaaaacacg 
      aaatatgta aatgcttta ttagtcttt tttggtaca 
      ttgtttgat tttccatgc gccaatcat gtaacgcaa 
      ccaaatcag gcctaaatc tttgcccat ctgaactga 
      gctccaaaa cggggtcag ttttgagcc ctcgatttc 
      agtccagtt aacagccac ttcaccaat tggaatgtg 
      cagtttttg ggcgatgta caacaactc gacgtcaaa 
      agccctaaa tacaaaata ataattctt aaaataaat 
      tatttaact ttcc