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Gene information
General information
TC007652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1550448..1577835
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007652
    iBeetle screen iB_01237

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection



    Pupal morphology
    wings shape irregular - Number of animals showing the phenotype: 4

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 3

    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007652 RA
      MSVYVDEEQ LVFDCEKKE LLGNSAPSR INKGGEPPT 
      ATMEAEKRI RREIANSNE RRRMQSINN GFQSLRSLL 
      PHHEGEKLS KAAILQQTA EYIYSLEQE KTRLLSQNC 
      QLKRLVSQH ESGGGELPP KKRKTEIIL PTITAESSD 
      EGLGSMSPE PVGLITVNV TSESPAELR RQLERERGL 
      RMVLEEQVR QFESQIYHQ QQNTQPHTI YQVEGTEEL 
      ALQLVSAEC LPPVGHTQT VVCSPPVSR SPSPEPEQR 
      LPSVLEAAI KAEPKVEVE RLPSPNPGH EDAAPRLYA 
      ANTSRQNLE TIVEAIRHL EGDHMFGDE PLSQEVPLA 
      LTKNERMNT LVEFSAAMV QQSQQSRPG VIVVKQAS
    CDS sequence
    • >TC007652 RA
      atgtctgtt tatgtcgac gaggagcag ctagtgttc 
      gactgtgag aaaaaggag cttttgggc aacagcgcg 
      ccgagtcga ataaacaaa ggcggtgaa ccacccacg 
      gcgacgatg gaggccgaa aagagaatc aggagagaa 
      atcgccaac agcaacgaa aggcggagg atgcagagc 
      ataaataat ggtttccaa tctctcagg tccttgttg 
      ccgcatcac gagggagaa aaattaagc aaggctgca 
      attctgcaa caaaccgcc gaatacatc tactctctt 
      gagcaggag aaaacgaga ctgttgtcg cagaactgc 
      cagctgaaa agactcgtt agtcaacat gaaagcggt 
      ggcggcgaa ttgccaccg aagaagcgc aagactgaa 
      attatttta cccacaata acggccgaa tcctctgac 
      gaagggctc gggtccatg tccccggag ccggtgggc 
      cttatcaca gtcaacgtc acctccgaa tctcctgcg 
      gaattacga aggcagctg gagcgggaa cgggggctt 
      cggatggtg cttgaagag caagtgcgt caatttgaa 
      agccaaatc taccaccag cagcaaaat actcaaccc 
      cataccatc taccaagtg gagggtacg gaagagtta 
      gccctccag ttagtgagc gctgagtgt ttgccgcca 
      gtgggacac acgcagacg gtggtgtgt tcgccccct 
      gtctcacga agccctagt cctgaaccg gaacaaagg 
      ctgccttcg gtgctggag gcggcgatc aaggccgag 
      cctaaggtg gaggtggag cgcctgccg tcgccaaac 
      ccgggccac gaggacgcc gcccctcgc ctatacgcc 
      gccaacacc tcgcgccag aacctggag accatcgtc 
      gaggcgatc cggcacctg gagggcgac cacatgttc 
      ggcgacgag ccgctgtcg caggaggtg ccgctcgcg 
      ttgacgaag aacgagcgg atgaacact ctggtggag 
      ttcagcgca gcgatggtc caacaaagc cagcagtcg 
      cggcctggg gttattgta gtcaagcag gcttcgtga
    mRNA sequence
    • >TC007652 RA
      acgaacatt tgctcccga cgactttcc tccgcgccg 
      taaatagta ccccaatgt aacggccct taaagtcgc 
      cctagagta ggttttttc gaaattagt gagacgttt 
      cgcgtgtgt tgtgccctt tagtggcgt gttgtgccg 
      caaacgcgg gccttttgc ctcaagtgg cgtaatcaa 
      gcgtgcgta tgaatatta cattggcgt caaccaggc 
      ctcagctgt ttcccgcca aacgccgct accagaaaa 
      ccgctgtga ggcgtagcg tatgtttgt catcccgcg 
      ctccagcgt actattagt gaaaaagtg agtaaattt 
      tttgcttga aagcgggcg aattgtcgg cggctgggg 
      ttgcgaaga ttatgtctg tttatgtcg acgaggagc 
      agctagtgt tcgactgtg agaaaaagg agcttttgg 
      gcaacagcg cgccgagtc gaataaaca aaggcggtg 
      aaccaccca cggcgacga tggaggccg aaaagagaa 
      tcaggagag aaatcgcca acagcaacg aaaggcgga 
      ggatgcaga gcataaata atggtttcc aatctctca 
      ggtccttgt tgccgcatc acgagggag aaaaattaa 
      gcaaggctg caattctgc aacaaaccg ccgaataca 
      tctactctc ttgagcagg agaaaacga gactgttgt 
      cgcagaact gccagctga aaagactcg ttagtcaac 
      atgaaagcg gtggcggcg aattgccac cgaagaagc 
      gcaagactg aaattattt tacccacaa taacggccg 
      aatcctctg acgaagggc tcgggtcca tgtccccgg 
      agccggtgg gccttatca cagtcaacg tcacctccg 
      aatctcctg cggaattac gaaggcagc tggagcggg 
      aacgggggc ttcggatgg tgcttgaag agcaagtgc 
      gtcaatttg aaagccaaa tctaccacc agcagcaaa 
      atactcaac cccatacca tctaccaag tggagggta 
      cggaagagt tagccctcc agttagtga gcgctgagt 
      gtttgccgc cagtgggac acacgcaga cggtggtgt 
      gttcgcccc ctgtctcac gaagcccta gtcctgaac 
      cggaacaaa ggctgcctt cggtgctgg aggcggcga 
      tcaaggccg agcctaagg tggaggtgg agcgcctgc 
      cgtcgccaa acccgggcc acgaggacg ccgcccctc 
      gcctatacg ccgccaaca cctcgcgcc agaacctgg 
      agaccatcg tcgaggcga tccggcacc tggagggcg 
      accacatgt tcggcgacg agccgctgt cgcaggagg 
      tgccgctcg cgttgacga agaacgagc ggatgaaca 
      ctctggtgg agttcagcg cagcgatgg tccaacaaa 
      gccagcagt cgcggcctg gggttattg tagtcaagc 
      aggcttcgt gatcccgca tcatacatt tcgcacgtt 
      cgtattcct gtaacgtgc caacaaaca gcatcccgg 
      aagggaagt cgggatctg tctccaaca aaagcaaac 
      agtgttagg tcggcctgc tgccgctgc tgaaacgcc 
      gccgttgtg ttcctacat cgtaaaatg cggacggcg 
      gagggagaa tgggacggc ggccggcca aggtttttg 
      aaaattcag attttttat aatatgacg gttttttta 
      cgatttttt tttgttact taaaaataa agtaatgtt 
      tctttttat ttgaaacgc caaaaatat atttatcat 
      aaaattaga aattgtagt gaaattgtg ttccttttg 
      tacatatat tttaacttt agggaagac gccgttagg 
      tcggcgctt agacatagc tgtattgta gcacttcag 
      gtccagtca tcaaacact tgaaaaaag gggcgctac 
      tgactattt taaaactat ttatcgacc tgttctaaa 
      atcatccaa ttaataaat caatttttt cataagaaa 
      atatagaca aattctgaa acaaaaaaa cattatgaa 
      ctgatttct gtgattatt aatttttga tatacaata 
      gactcgtat gtggtttaa aggcattga caattttat 
      ttttttatg agattaatt tgaacgttg ttttatcat 
      agaaaaaaa cacgttact caatacgaa ttgatagcc 
      gaaattgtt acattactc aattggggc aaaaaccaa 
      gctggcgcc ccttttgcc tctcgtggt tgggctgca 
      tagagaatt acgtttcat ttcagttcg tttgttgtt 
      ttctttgtt cgagattgt gtttttcca aatgtatat 
      actaaaaaa ttagtatac gtataatga agtttgaga 
      atattaatg attgttttt cgttacatt agtaggata 
      attgagttc tgatgctga tgatcaggg cacatgccg 
      acacacatt caaatagta ataatgtgg tggcaagag 
      atcccgttt gttgcctct ctgtgaact gtacttctg 
      tcatacctg catgagtgt gtatttgga gtgtgctga 
      gtttctttt tatactaaa aaaatttcg agaataaat 
      tggttccaa gcttgactc gggacagta ctgaaattc 
      tgagtaact gcgataaaa tgagtaata aattagatt 
      aggtagggt ttggaaatg tgacttgtg tggtggccg 
      ttgtttata tttttgccg aagtaggaa attgttgaa 
      ggggttcta gtgaaggga aggtgacca cccgcaagg 
      tcttgttgt tggttggga gttgagtgt ctgtctact 
      tgcatcatc caatcgaac ggttaggaa aattttgca 
      ctcagtcgt ggctgttca gagaaagtt cctcgattt 
      tccgttcat ttatttacg tttacattt ttacgttta 
      gctttcagg gcaaggctt ctcttttta cccctaagt 
      atttacagt tatgcaaga ctgggtgta gacatagta 
      ataattatt atatagatg tacttctaa gtctacttt 
      gtaactatt ggatggatg taaagtaag tcgataact 
      aaattaacg tttctttga ttggttccc attattact 
      gtgattgta tttatataa aaattgata acattataa 
      tgctaattt agtatatgt actagccac aggctattt 
      tgcgggctt gtatctgtt gaaactgtt gcttttcat 
      acagcactc cattattgt atgtgttag cagcaactg 
      attatcaca aatgatgat cagtcgctg attatgtct 
      ttcgttgaa gctccaatg ttattgcaa catacattt 
      tattttgag tttttacac gttttcaaa acaatttta 
      tgttgcagt aactttgat cgtttctga taaggtctg 
      gtccgacaa aataatcac tgcacacat taacttatt 
      atggttatg tgcttcgat cattatgtt gtgtcgct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY