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Gene information
General information
TC007650 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 1587620..1590561
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007650
    iBeetle screen iB_01236
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    abdominal odoriferous glands shape irregular - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    abdominal odoriferous glands colour not present - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection



    Fertility
    egg production: number no/almost no eggs -
    ovariole not present - Number of animals showing the phenotype: 4

    ovary size decreased - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007650 RA
      MDERVPRIG EIRIMVRPE YYLSKARQE LANGNKIGA 
      IQHYNSYVE NLRTPHDMP LVEQITLTK LVMSLSNNV 
      IEPSPEVEN EILKCYLTM LNSFPANAI LLNAFGVYF 
      FAQGEYTVA RHYLQKAAS LGYLPAEKN YLHVIWHLI 
      PRWHFRMLN DRKRNEFFR DAIHRAIDA GFKDVYDIG 
      CGCGILTLI AATYRKDVR VTSIEENKI LYDLTDTLV 
      KKRNFNIRL VNGNSNTIT TRPAPCNLI VTEIFDAGV 
      FGEDCLKTI HHALEYFVD KKFRIIPAS AKLYVTAFE 
      SRELTRKFR YTESLKELK LENICLVEV DPEPYDAEY 
      LTKKPVKFL SDTKAFLEV KFYNKAQLN DLLTPDSDC 
      IGEIELVCS EKGTIHALA VWFDLRLDA EITITTNPF 
      IPSNTKCWE QAIVHLDHP IEVAAGDTL RLKPRIVEG 
      QIHFDVLNH ENTCEKCFE VSREVISFL NDTKLVQSV 
      IKAADSYEH CDLRIIDFN VFPLFGFLM AKKGATVYH 
      VYNDESDLT LFNHIVNLN ELPVQRFIF IYYRAVEDY 
      LVFLEPPHL VFYELVKPD GSWSRPEYS LEMEAKLTC 
      AYLPQKVFL HAQLISSDY LTLCNKVDD KKALNFKIA 
      DLINAYSGN EHPNLETLE HKKHSSVVT FDISDLYRY 
      DFHMEADII KSGSCNGIL YWFDIQFTA DPSNVFNTL 
      TSTHYDRSC TLLIDKPKV VKAGEKVMI HIMRLEGFL 
      KLYCD
    CDS sequence
    • >TC007650 RA
      atggatgag cgtgtgccc cgcattggg gaaatccgg 
      attatggtc cggcccgag tactatctc tcgaaggcg 
      cggcaagag ctcgccaac ggtaacaaa atcggggct 
      atccagcac tacaacagc tacgtcgag aacttgcgc 
      accccgcac gacatgccc ctcgtcgag caaatcact 
      ctaaccaag ctcgtgatg agtctcagt aacaacgtg 
      attgagcca agtcccgag gtcgaaaac gagatcctc 
      aagtgttac ctcacgatg ctcaacagt ttcccggcc 
      aatgccatt ctgttgaac gccttcggg gtctacttt 
      ttcgcccaa ggggagtac accgtagct agacattac 
      ttgcagaag gcggcaagt ctgggttac ctcccggct 
      gagaaaaac tacctgcac gtgatctgg catttgata 
      ccgcgctgg cacttccgc atgttaaat gaccggaag 
      cggaacgaa tttttccga gatgccatt catcgggcc 
      attgatgcg gggtttaag gacgtgtat gatatcggg 
      tgcggttgc ggcattttg accctaatt gcggcaacg 
      tatcgcaaa gacgttcga gtcactagt attgaagag 
      aacaaaatc ttgtacgat ttaaccgat actttggtc 
      aaaaaacgt aatttcaat attcgccta gttaacggt 
      aactctaac acgattaca acgcgccca gcgccctgt 
      aatttgata gtaacggag atttttgac gcgggggtt 
      tttggggaa gactgtctc aagaccatt caccacgca 
      cttgaatac ttcgttgat aaaaagttc agaatcatt 
      ccggcaagt gccaagctc tatgtcacc gccttcgag 
      agtcgggaa ctcacccgg aagttccgc tacaccgag 
      tctctgaag gagctcaag cttgagaac atttgcctg 
      gttgaggtc gatcccgag ccgtatgac gccgagtac 
      ttgaccaaa aaaccggtc aagtttttg tcggatact 
      aaggcgttc ctcgaagtt aaattctac aataaggcg 
      caattgaac gatttatta acacctgat agcgactgc 
      atcggcgaa attgagttg gtttgttcc gaaaagggc 
      acaattcac gcattggcc gtttggttt gatctccgg 
      ctagacgca gaaatcacg attacgaca aacccgttc 
      attccctca aacaccaag tgctgggag caggccatt 
      gtccacctg gaccacccg atcgaagtc gccgcgggt 
      gacactctc cggctgaaa ccgcgcatt gtcgagggc 
      cagatacat ttcgatgtt ttgaaccac gaaaacacg 
      tgtgaaaaa tgctttgaa gtcagtaga gaagtaata 
      tcgtttctt aacgacact aaactagtc cagagtgtg 
      atcaaggct gctgatagt tacgaacat tgcgatctc 
      aggattata gatttcaat gtttttccc ttgtttggg 
      tttttgatg gcgaaaaaa ggggcgacg gtctatcat 
      gtctacaac gatgagagc gacttgact ttgttcaat 
      catatcgtg aatctgaac gagttgccg gttcaaaga 
      tttattttt atttactat cgggccgtt gaggattat 
      ttggtgttt ttggagccg ccccattta gttttctac 
      gagttggtc aaaccggac gggtcctgg agcaggccc 
      gagtattcg cttgaaatg gaagctaag ctaacttgt 
      gcctatttg ccgcagaag gtttttcta cacgcacaa 
      ctcatcagc tcggattat ttgaccctt tgtaataaa 
      gtcgatgac aagaaggcg ctcaatttc aagattgcc 
      gatctaatc aacgcctac tcgggcaac gaacatccc 
      aaccttgag acgcttgaa cacaagaag cactcatct 
      gttgtcact ttcgacatc agcgatttg tacaggtat 
      gacttccac atggaagcg gatataatc aagagtggc 
      tcctgcaat ggaattctc tactggttt gatatccag 
      ttcactgct gatccaagt aacgtgttc aacactttg 
      accagcacc cattacgac aggtcctgc accctcctc 
      atcgataaa cccaaagta gtcaaagcc ggtgaaaaa 
      gtaatgatc cacataatg agactcgag ggattttta 
      aaactgtat tgtgattaa
    mRNA sequence
    • >TC007650 RA
      caaaaaacg gtgtaagaa gctcctgca cgttatttg 
      cccgatttg tgtgatttt acgatggat atggatgag 
      cgtgtgccc cgcattggg gaaatccgg attatggtc 
      cggcccgag tactatctc tcgaaggcg cggcaagag 
      ctcgccaac ggtaacaaa atcggggct atccagcac 
      tacaacagc tacgtcgag aacttgcgc accccgcac 
      gacatgccc ctcgtcgag caaatcact ctaaccaag 
      ctcgtgatg agtctcagt aacaacgtg attgagcca 
      agtcccgag gtcgaaaac gagatcctc aagtgttac 
      ctcacgatg ctcaacagt ttcccggcc aatgccatt 
      ctgttgaac gccttcggg gtctacttt ttcgcccaa 
      ggggagtac accgtagct agacattac ttgcagaag 
      gcggcaagt ctgggttac ctcccggct gagaaaaac 
      tacctgcac gtgatctgg catttgata ccgcgctgg 
      cacttccgc atgttaaat gaccggaag cggaacgaa 
      tttttccga gatgccatt catcgggcc attgatgcg 
      gggtttaag gacgtgtat gatatcggg tgcggttgc 
      ggcattttg accctaatt gcggcaacg tatcgcaaa 
      gacgttcga gtcactagt attgaagag aacaaaatc 
      ttgtacgat ttaaccgat actttggtc aaaaaacgt 
      aatttcaat attcgccta gttaacggt aactctaac 
      acgattaca acgcgccca gcgccctgt aatttgata 
      gtaacggag atttttgac gcgggggtt tttggggaa 
      gactgtctc aagaccatt caccacgca cttgaatac 
      ttcgttgat aaaaagttc agaatcatt ccggcaagt 
      gccaagctc tatgtcacc gccttcgag agtcgggaa 
      ctcacccgg aagttccgc tacaccgag tctctgaag 
      gagctcaag cttgagaac atttgcctg gttgaggtc 
      gatcccgag ccgtatgac gccgagtac ttgaccaaa 
      aaaccggtc aagtttttg tcggatact aaggcgttc 
      ctcgaagtt aaattctac aataaggcg caattgaac 
      gatttatta acacctgat agcgactgc atcggcgaa 
      attgagttg gtttgttcc gaaaagggc acaattcac 
      gcattggcc gtttggttt gatctccgg ctagacgca 
      gaaatcacg attacgaca aacccgttc attccctca 
      aacaccaag tgctgggag caggccatt gtccacctg 
      gaccacccg atcgaagtc gccgcgggt gacactctc 
      cggctgaaa ccgcgcatt gtcgagggc cagatacat 
      ttcgatgtt ttgaaccac gaaaacacg tgtgaaaaa 
      tgctttgaa gtcagtaga gaagtaata tcgtttctt 
      aacgacact aaactagtc cagagtgtg atcaaggct 
      gctgatagt tacgaacat tgcgatctc aggattata 
      gatttcaat gtttttccc ttgtttggg tttttgatg 
      gcgaaaaaa ggggcgacg gtctatcat gtctacaac 
      gatgagagc gacttgact ttgttcaat catatcgtg 
      aatctgaac gagttgccg gttcaaaga tttattttt 
      atttactat cgggccgtt gaggattat ttggtgttt 
      ttggagccg ccccattta gttttctac gagttggtc 
      aaaccggac gggtcctgg agcaggccc gagtattcg 
      cttgaaatg gaagctaag ctaacttgt gcctatttg 
      ccgcagaag gtttttcta cacgcacaa ctcatcagc 
      tcggattat ttgaccctt tgtaataaa gtcgatgac 
      aagaaggcg ctcaatttc aagattgcc gatctaatc 
      aacgcctac tcgggcaac gaacatccc aaccttgag 
      acgcttgaa cacaagaag cactcatct gttgtcact 
      ttcgacatc agcgatttg tacaggtat gacttccac 
      atggaagcg gatataatc aagagtggc tcctgcaat 
      ggaattctc tactggttt gatatccag ttcactgct 
      gatccaagt aacgtgttc aacactttg accagcacc 
      cattacgac aggtcctgc accctcctc atcgataaa 
      cccaaagta gtcaaagcc ggtgaaaaa gtaatgatc 
      cacataatg agactcgag ggattttta aaactgtat 
      tgtgattaa ttgtgtaat gtattcgaa aactgtgat 
      gtttactat taggtgtgt taatttgtt tcgcacttt 
      gcgacacta attgtaata ttttgtact tgaattgtt 
      taaacaggt ctcagggta aaagaaaat gtaaaaatt 
      tgcataatt tattaaaac catcaattg tca