Status
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Gene information
General information
TC007647 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1602644..1605241
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007647
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01235

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007647 RA
      MGEEEEEHQ ENEDNDWED HDDNDWEDY DEPEEVEEE 
      EEEDTSGED SHLFSDSSD DDSTVILDT DAEVDFENH 
      PLFKKEKPK HKWFVLKEV DQRQLGYSA KLQSRELFQ 
      RRCYGSLHC VQRLELMYK LEEHAGCVN SLNFHPDGT 
      LLASGADDL KVVVWDWKL GKTLLKYKT KHRANVFQS 
      KFLHLYGDL HIATCARDG QVRLAQVNK EEGVRNARL 
      LGSHKGPCH KLIVLPEQP HIILSAGED GAVLNHDVR 
      DPKSTKVVT VKEDSKTIA LYSIHGHPL KSHEFCVSG 
      RDSIVRVYD QRKSNKPAA TYTPFVKAK AKNHRNYHV 
      TCAVYNHNG SEILASYSE SDVFLFDVN DTEPGKFIH 
      QYQGHKNGA TIKGVNFFG PKSEFVVSG SDCGHIYFW 
      ERNSEALVQ WLLADDNGV VNCLEPHPQ LPFICTSGL 
      DWDVKVWVP SCEVEPKME GLSDTIKDN LKMKLGHEG 
      TEFNENRML WILWRHLRN TTRLRASNM LNIRDFLQL 
      YSDSNSSGS ESVANSDGS DDDLDRPPN CNTS
    • >TC007647 RB
      MGEEEEEHQ ENEDNDWED HDDNDWEDY DEPEEVEEE 
      EEEDTSGED SHLFSDSSD DDSTVILDT DAEVDFENH 
      PLFKKEKPK HKWFVLKEV DQRQLGYSA KLQSRELFQ 
      RRCYGSLHC VQRLELMYK LEEHAGCVN SLNFHPDGT 
      LLASGADDL KVVVWDWKL GKTLLKYKT KHRANVFQS 
      KFLHLYGDL HIATCARDG QVRLAQVNK EEGVRNARL 
      LGSHKGPCH KLIVLPEQP HIILSAGED GAVLNHDVR 
      DPKSTKVVT VKEDSKTIA LYSIHGHPL KSHEFCVSG 
      RDSIVRVYD QRKSNKPAA TYTPFAKAK NHRNYHVTC 
      AVYNHNGSE ILASYSESD VFLFDVNDT EPGKFIHQY 
      QGHKNGATI KGVNFFGPK SEFVVSGSD CGHIYFWER 
      NSEALVQWL LADDNGVVN CLEPHPQLP FICTSGLDW 
      DVKVWVPSC EVEPKMEGL SDTIKDNLK MKLGHEGTE 
      FNENRMLWI LWRHLRNTT RLRASNMLN IRDFLQLYS 
      DSNSSGSES VANSDGSDD DLDRPPNCN TS
    • >TC007647 RC
      MGEEEEEHQ ENEDNDWED HDDNDWEDY DEPEEVEEE 
      EEEDTSDAE VDFENHPLF KKEKPKHKW FVLKEVDQR 
      QLGYSAKLQ SRELFQRRC YGSLHCVQR LELMYKLEE 
      HAGCVNSLN FHPDGTLLA SGADDLKVV VWDWKLGKT 
      LLKYKTKHR ANVFQSKFL HLYGDLHIA TCARDGQVR 
      LAQVNKEEG VRNARLLGS HKGPCHKLI VLPEQPHII 
      LSAGEDGAV LNHDVRDPK STKVVTVKE DSKTIALYS 
      IHGHPLKSH EFCVSGRDS IVRVYDQRK SNKPAATYT 
      PFVKAKAKN HRNYHVTCA VYNHNGSEI LASYSESDV 
      FLFDVNDTE PGKFIHQYQ GHKNGATIK GVNFFGPKS 
      EFVVSGSDC GHIYFWERN SEALVQWLL ADDNGVVNC 
      LEPHPQLPF ICTSGLDWD VKVWVPSCE VEPKMEGLS 
      DTIKDNLKM KLGHEGTEF NENRMLWIL WRHLRNTTR 
      LRASNMLNI RDFLQLYSD SNSSGSESV ANSDGSDDD 
      LDRPPNCNT S
    CDS sequence
    • >TC007647 RA
      atgggggaa gaagaggaa gaacaccag gagaatgaa 
      gacaacgac tgggaagac catgacgat aacgactgg 
      gaggattac gacgagcct gaggaggtg gaggaggag 
      gaagaagag gacacgtca ggtgaagac tctcatttg 
      ttcagtgac agcagtgat gatgactcc actgttatc 
      cttgataca gatgctgaa gtcgatttc gaaaaccac 
      ccgcttttc aaaaaagag aagcccaag cacaagtgg 
      ttcgttctc aaggaagtt gatcaaaga cagttaggt 
      tacagtgca aagttgcaa agtcgcgag ctgtttcaa 
      aggcggtgc tacggctcc ctacattgt gtacagcgc 
      ttagagtta atgtataag ttagaggag catgcaggt 
      tgtgtaaat agcttgaat tttcacccc gatggtacg 
      ctcttggcc agtggggcc gatgacctg aaagtggtg 
      gtttgggac tggaagttg ggtaaaacc ttgctgaag 
      tacaaaacg aagcacagg gccaacgtc tttcaaagc 
      aaattcttg catctttat ggggattta catatcgcg 
      acgtgcgcc agagatgga caggtacgg ctggcacaa 
      gtgaataag gaggaaggc gtacgtaac gccaggctt 
      ttggggtcg cataagggg ccctgtcat aagctcatc 
      gtgcttcca gaacaacca cacattatt ttaagcgca 
      ggcgaggat ggggcggtt ctcaaccat gatgtcaga 
      gaccccaaa agcaccaaa gtagttaca gtaaaagag 
      gattcaaag acaatagct ttatacagt atccacggc 
      cacccgcta aagtcgcac gagttttgc gttagtggt 
      agagattca attgttaga gtttacgac caaagaaaa 
      agtaacaag cctgctgcg acgtacacg ccttttgta 
      aaggctaaa gcaaagaat cataggaat tatcacgtc 
      acttgtgct gtatacaac cacaacgga tctgaaatc 
      ctggcatcg tacagcgaa tccgatgtt tttctcttc 
      gatgttaac gacaccgaa cctgggaaa tttatccat 
      cagtaccag ggtcacaaa aacggagcc acgattaaa 
      ggtgttaac tttttcggg cccaagtcg gaatttgta 
      gtgagtggg tcagattgt ggccacatt tatttctgg 
      gagcggaat agtgaagcg ctagtgcag tggttactt 
      gccgatgat aatggagtt gttaattgt ttggagcct 
      cacccccaa ttacctttt atttgtacg agtggtttg 
      gattgggac gttaaagtt tgggttccg tcctgtgaa 
      gtggaacca aaaatggaa ggtttgagt gacacaata 
      aaagataat ttaaaaatg aaactaggt catgaagga 
      acggaattt aacgaaaat cggatgttg tggattctg 
      tggcgacat ctcaggaac accactaga ctaagggcg 
      tctaatatg cttaacatt agagatttt ctgcagctt 
      tattcagac tcgaactcc tcaggatcg gagagcgtt 
      gcgaattct gacggttcc gacgacgat cttgatagg 
      ccaccaaac tgtaatact tcttaa
    • >TC007647 RB
      atgggggaa gaagaggaa gaacaccag gagaatgaa 
      gacaacgac tgggaagac catgacgat aacgactgg 
      gaggattac gacgagcct gaggaggtg gaggaggag 
      gaagaagag gacacgtca ggtgaagac tctcatttg 
      ttcagtgac agcagtgat gatgactcc actgttatc 
      cttgataca gatgctgaa gtcgatttc gaaaaccac 
      ccgcttttc aaaaaagag aagcccaag cacaagtgg 
      ttcgttctc aaggaagtt gatcaaaga cagttaggt 
      tacagtgca aagttgcaa agtcgcgag ctgtttcaa 
      aggcggtgc tacggctcc ctacattgt gtacagcgc 
      ttagagtta atgtataag ttagaggag catgcaggt 
      tgtgtaaat agcttgaat tttcacccc gatggtacg 
      ctcttggcc agtggggcc gatgacctg aaagtggtg 
      gtttgggac tggaagttg ggtaaaacc ttgctgaag 
      tacaaaacg aagcacagg gccaacgtc tttcaaagc 
      aaattcttg catctttat ggggattta catatcgcg 
      acgtgcgcc agagatgga caggtacgg ctggcacaa 
      gtgaataag gaggaaggc gtacgtaac gccaggctt 
      ttggggtcg cataagggg ccctgtcat aagctcatc 
      gtgcttcca gaacaacca cacattatt ttaagcgca 
      ggcgaggat ggggcggtt ctcaaccat gatgtcaga 
      gaccccaaa agcaccaaa gtagttaca gtaaaagag 
      gattcaaag acaatagct ttatacagt atccacggc 
      cacccgcta aagtcgcac gagttttgc gttagtggt 
      agagattca attgttaga gtttacgac caaagaaaa 
      agtaacaag cctgctgcg acgtacacg ccttttgct 
      aaagcaaag aatcatagg aattatcac gtcacttgt 
      gctgtatac aaccacaac ggatctgaa atcctggca 
      tcgtacagc gaatccgat gtttttctc ttcgatgtt 
      aacgacacc gaacctggg aaatttatc catcagtac 
      cagggtcac aaaaacgga gccacgatt aaaggtgtt 
      aactttttc gggcccaag tcggaattt gtagtgagt 
      gggtcagat tgtggccac atttatttc tgggagcgg 
      aatagtgaa gcgctagtg cagtggtta cttgccgat 
      gataatgga gttgttaat tgtttggag cctcacccc 
      caattacct tttatttgt acgagtggt ttggattgg 
      gacgttaaa gtttgggtt ccgtcctgt gaagtggaa 
      ccaaaaatg gaaggtttg agtgacaca ataaaagat 
      aatttaaaa atgaaacta ggtcatgaa ggaacggaa 
      tttaacgaa aatcggatg ttgtggatt ctgtggcga 
      catctcagg aacaccact agactaagg gcgtctaat 
      atgcttaac attagagat tttctgcag ctttattca 
      gactcgaac tcctcagga tcggagagc gttgcgaat 
      tctgacggt tccgacgac gatcttgat aggccacca 
      aactgtaat acttcttaa
    • >TC007647 RC
      atgggggaa gaagaggaa gaacaccag gagaatgaa 
      gacaacgac tgggaagac catgacgat aacgactgg 
      gaggattac gacgagcct gaggaggtg gaggaggag 
      gaagaagag gacacgtca gatgctgaa gtcgatttc 
      gaaaaccac ccgcttttc aaaaaagag aagcccaag 
      cacaagtgg ttcgttctc aaggaagtt gatcaaaga 
      cagttaggt tacagtgca aagttgcaa agtcgcgag 
      ctgtttcaa aggcggtgc tacggctcc ctacattgt 
      gtacagcgc ttagagtta atgtataag ttagaggag 
      catgcaggt tgtgtaaat agcttgaat tttcacccc 
      gatggtacg ctcttggcc agtggggcc gatgacctg 
      aaagtggtg gtttgggac tggaagttg ggtaaaacc 
      ttgctgaag tacaaaacg aagcacagg gccaacgtc 
      tttcaaagc aaattcttg catctttat ggggattta 
      catatcgcg acgtgcgcc agagatgga caggtacgg 
      ctggcacaa gtgaataag gaggaaggc gtacgtaac 
      gccaggctt ttggggtcg cataagggg ccctgtcat 
      aagctcatc gtgcttcca gaacaacca cacattatt 
      ttaagcgca ggcgaggat ggggcggtt ctcaaccat 
      gatgtcaga gaccccaaa agcaccaaa gtagttaca 
      gtaaaagag gattcaaag acaatagct ttatacagt 
      atccacggc cacccgcta aagtcgcac gagttttgc 
      gttagtggt agagattca attgttaga gtttacgac 
      caaagaaaa agtaacaag cctgctgcg acgtacacg 
      ccttttgta aaggctaaa gcaaagaat cataggaat 
      tatcacgtc acttgtgct gtatacaac cacaacgga 
      tctgaaatc ctggcatcg tacagcgaa tccgatgtt 
      tttctcttc gatgttaac gacaccgaa cctgggaaa 
      tttatccat cagtaccag ggtcacaaa aacggagcc 
      acgattaaa ggtgttaac tttttcggg cccaagtcg 
      gaatttgta gtgagtggg tcagattgt ggccacatt 
      tatttctgg gagcggaat agtgaagcg ctagtgcag 
      tggttactt gccgatgat aatggagtt gttaattgt 
      ttggagcct cacccccaa ttacctttt atttgtacg 
      agtggtttg gattgggac gttaaagtt tgggttccg 
      tcctgtgaa gtggaacca aaaatggaa ggtttgagt 
      gacacaata aaagataat ttaaaaatg aaactaggt 
      catgaagga acggaattt aacgaaaat cggatgttg 
      tggattctg tggcgacat ctcaggaac accactaga 
      ctaagggcg tctaatatg cttaacatt agagatttt 
      ctgcagctt tattcagac tcgaactcc tcaggatcg 
      gagagcgtt gcgaattct gacggttcc gacgacgat 
      cttgatagg ccaccaaac tgtaatact tcttaa
    mRNA sequence
    • >TC007647 RA
      tcacctgtt ttatttttt ccttgcatt ggcattact 
      ttgattttt gtttttgtg ccgaatttg acagctctg 
      ttacttttt atttaatga aaaacaacc catctgtat 
      ttaatttcc cacaaaact gcgacattt actaggtga 
      gtaatcgtc caaatgtgg gtaaaataa tcgcgttga 
      gaaatcgtc cctcgtaag tgtgcccgt gtcaagtgt 
      tgggaaact acgtctccg ttatatatt tttagaata 
      aatatggat gataaggag aaagacaac aagaacaag 
      tcaaacgat gaggacaaa tcgaaggaa aatgccaaa 
      cggttcaaa accagcgaa gagtcgagc tctagcaac 
      actgctagc actagtgct ggggaggca gacaaccct 
      ccagagccc cccgtgagg ttcaactcc acccccaac 
      tcccgaaac cgcaattac cggaacagt cgcgagaat 
      tttgaggaa gaaagtggc gatagggga cgatcccat 
      gggggaaga agaggaaga acaccagga gaatgaaga 
      caacgactg ggaagacca tgacgataa cgactggga 
      ggattacga cgagcctga ggaggtgga ggaggagga 
      agaagagga cacgtcagg tgaagactc tcatttgtt 
      cagtgacag cagtgatga tgactccac tgttatcct 
      tgatacaga tgctgaagt cgatttcga aaaccaccc 
      gcttttcaa aaaagagaa gcccaagca caagtggtt 
      cgttctcaa ggaagttga tcaaagaca gttaggtta 
      cagtgcaaa gttgcaaag tcgcgagct gtttcaaag 
      gcggtgcta cggctccct acattgtgt acagcgctt 
      agagttaat gtataagtt agaggagca tgcaggttg 
      tgtaaatag cttgaattt tcaccccga tggtacgct 
      cttggccag tggggccga tgacctgaa agtggtggt 
      ttgggactg gaagttggg taaaacctt gctgaagta 
      caaaacgaa gcacagggc caacgtctt tcaaagcaa 
      attcttgca tctttatgg ggatttaca tatcgcgac 
      gtgcgccag agatggaca ggtacggct ggcacaagt 
      gaataagga ggaaggcgt acgtaacgc caggctttt 
      ggggtcgca taaggggcc ctgtcataa gctcatcgt 
      gcttccaga acaaccaca cattatttt aagcgcagg 
      cgaggatgg ggcggttct caaccatga tgtcagaga 
      ccccaaaag caccaaagt agttacagt aaaagagga 
      ttcaaagac aatagcttt atacagtat ccacggcca 
      cccgctaaa gtcgcacga gttttgcgt tagtggtag 
      agattcaat tgttagagt ttacgacca aagaaaaag 
      taacaagcc tgctgcgac gtacacgcc ttttgtaaa 
      ggctaaagc aaagaatca taggaatta tcacgtcac 
      ttgtgctgt atacaacca caacggatc tgaaatcct 
      ggcatcgta cagcgaatc cgatgtttt tctcttcga 
      tgttaacga caccgaacc tgggaaatt tatccatca 
      gtaccaggg tcacaaaaa cggagccac gattaaagg 
      tgttaactt tttcgggcc caagtcgga atttgtagt 
      gagtgggtc agattgtgg ccacattta tttctggga 
      gcggaatag tgaagcgct agtgcagtg gttacttgc 
      cgatgataa tggagttgt taattgttt ggagcctca 
      cccccaatt accttttat ttgtacgag tggtttgga 
      ttgggacgt taaagtttg ggttccgtc ctgtgaagt 
      ggaaccaaa aatggaagg tttgagtga cacaataaa 
      agataattt aaaaatgaa actaggtca tgaaggaac 
      ggaatttaa cgaaaatcg gatgttgtg gattctgtg 
      gcgacatct caggaacac cactagact aagggcgtc 
      taatatgct taacattag agattttct gcagcttta 
      ttcagactc gaactcctc aggatcgga gagcgttgc 
      gaattctga cggttccga cgacgatct tgataggcc 
      accaaactg taatacttc ttaatcaac gaaatttgt 
      ttttgtagc atttatttt tattttgtt a
    • >TC007647 RB
      tcacctgtt ttatttttt ccttgcatt ggcattact 
      ttgattttt gtttttgtg ccgaatttg acagctctg 
      ttacttttt atttaatga aaaacaacc catctgtat 
      ttaatttcc cacaaaact gcgacattt actaggtga 
      gtaatcgtc caaatgtgg gtaaaataa tcgcgttga 
      gaaatcgtc cctcgtaag tgtgcccgt gtcaagtgt 
      tgggaaact acgtctccg ttatatatt tttagaata 
      aatatggat gataaggag aaagacaac aagaacaag 
      tcaaacgat gaggacaaa tcgaaggaa aatgccaaa 
      cggttcaaa accagcgaa gagtcgagc tctagcaac 
      actgctagc actagtgct ggggaggca gacaaccct 
      ccagagccc cccgtgagg ttcaactcc acccccaac 
      tcccgaaac cgcaattac cggaacagt cgcgagaat 
      tttgaggaa gaaagtggc gatagggga cgatcccat 
      gggggaaga agaggaaga acaccagga gaatgaaga 
      caacgactg ggaagacca tgacgataa cgactggga 
      ggattacga cgagcctga ggaggtgga ggaggagga 
      agaagagga cacgtcagg tgaagactc tcatttgtt 
      cagtgacag cagtgatga tgactccac tgttatcct 
      tgatacaga tgctgaagt cgatttcga aaaccaccc 
      gcttttcaa aaaagagaa gcccaagca caagtggtt 
      cgttctcaa ggaagttga tcaaagaca gttaggtta 
      cagtgcaaa gttgcaaag tcgcgagct gtttcaaag 
      gcggtgcta cggctccct acattgtgt acagcgctt 
      agagttaat gtataagtt agaggagca tgcaggttg 
      tgtaaatag cttgaattt tcaccccga tggtacgct 
      cttggccag tggggccga tgacctgaa agtggtggt 
      ttgggactg gaagttggg taaaacctt gctgaagta 
      caaaacgaa gcacagggc caacgtctt tcaaagcaa 
      attcttgca tctttatgg ggatttaca tatcgcgac 
      gtgcgccag agatggaca ggtacggct ggcacaagt 
      gaataagga ggaaggcgt acgtaacgc caggctttt 
      ggggtcgca taaggggcc ctgtcataa gctcatcgt 
      gcttccaga acaaccaca cattatttt aagcgcagg 
      cgaggatgg ggcggttct caaccatga tgtcagaga 
      ccccaaaag caccaaagt agttacagt aaaagagga 
      ttcaaagac aatagcttt atacagtat ccacggcca 
      cccgctaaa gtcgcacga gttttgcgt tagtggtag 
      agattcaat tgttagagt ttacgacca aagaaaaag 
      taacaagcc tgctgcgac gtacacgcc ttttgctaa 
      agcaaagaa tcataggaa ttatcacgt cacttgtgc 
      tgtatacaa ccacaacgg atctgaaat cctggcatc 
      gtacagcga atccgatgt ttttctctt cgatgttaa 
      cgacaccga acctgggaa atttatcca tcagtacca 
      gggtcacaa aaacggagc cacgattaa aggtgttaa 
      ctttttcgg gcccaagtc ggaatttgt agtgagtgg 
      gtcagattg tggccacat ttatttctg ggagcggaa 
      tagtgaagc gctagtgca gtggttact tgccgatga 
      taatggagt tgttaattg tttggagcc tcaccccca 
      attaccttt tatttgtac gagtggttt ggattggga 
      cgttaaagt ttgggttcc gtcctgtga agtggaacc 
      aaaaatgga aggtttgag tgacacaat aaaagataa 
      tttaaaaat gaaactagg tcatgaagg aacggaatt 
      taacgaaaa tcggatgtt gtggattct gtggcgaca 
      tctcaggaa caccactag actaagggc gtctaatat 
      gcttaacat tagagattt tctgcagct ttattcaga 
      ctcgaactc ctcaggatc ggagagcgt tgcgaattc 
      tgacggttc cgacgacga tcttgatag gccaccaaa 
      ctgtaatac ttcttaatc aacgaaatt tgtttttgt 
      agcatttat ttttatttt gtta
    • >TC007647 RC
      tcacctgtt ttatttttt ccttgcatt ggcattact 
      ttgattttt gtttttgtg ccgaatttg acagctctg 
      ttacttttt atttaatga aaaacaacc catctgtat 
      ttaatttcc cacaaaact gcgacattt actaggtga 
      gtaatcgtc caaatgtgg gtaaaataa tcgcgttga 
      gaaatcgtc cctcgtaag tgtgcccgt gtcaagtgt 
      tgggaaact acgtctccg ttatatatt tttagaata 
      aatatggat gataaggag aaagacaac aagaacaag 
      tcaaacgat gaggacaaa tcgaaggaa aatgccaaa 
      cggttcaaa accagcgaa gagtcgagc tctagcaac 
      actgctagc actagtgct ggggaggca gacaaccct 
      ccagagccc cccgtgagg ttcaactcc acccccaac 
      tcccgaaac cgcaattac cggaacagt cgcgagaat 
      tttgaggaa gaaagtggc gatagggga cgatcccat 
      gggggaaga agaggaaga acaccagga gaatgaaga 
      caacgactg ggaagacca tgacgataa cgactggga 
      ggattacga cgagcctga ggaggtgga ggaggagga 
      agaagagga cacgtcaga tgctgaagt cgatttcga 
      aaaccaccc gcttttcaa aaaagagaa gcccaagca 
      caagtggtt cgttctcaa ggaagttga tcaaagaca 
      gttaggtta cagtgcaaa gttgcaaag tcgcgagct 
      gtttcaaag gcggtgcta cggctccct acattgtgt 
      acagcgctt agagttaat gtataagtt agaggagca 
      tgcaggttg tgtaaatag cttgaattt tcaccccga 
      tggtacgct cttggccag tggggccga tgacctgaa 
      agtggtggt ttgggactg gaagttggg taaaacctt 
      gctgaagta caaaacgaa gcacagggc caacgtctt 
      tcaaagcaa attcttgca tctttatgg ggatttaca 
      tatcgcgac gtgcgccag agatggaca ggtacggct 
      ggcacaagt gaataagga ggaaggcgt acgtaacgc 
      caggctttt ggggtcgca taaggggcc ctgtcataa 
      gctcatcgt gcttccaga acaaccaca cattatttt 
      aagcgcagg cgaggatgg ggcggttct caaccatga 
      tgtcagaga ccccaaaag caccaaagt agttacagt 
      aaaagagga ttcaaagac aatagcttt atacagtat 
      ccacggcca cccgctaaa gtcgcacga gttttgcgt 
      tagtggtag agattcaat tgttagagt ttacgacca 
      aagaaaaag taacaagcc tgctgcgac gtacacgcc 
      ttttgtaaa ggctaaagc aaagaatca taggaatta 
      tcacgtcac ttgtgctgt atacaacca caacggatc 
      tgaaatcct ggcatcgta cagcgaatc cgatgtttt 
      tctcttcga tgttaacga caccgaacc tgggaaatt 
      tatccatca gtaccaggg tcacaaaaa cggagccac 
      gattaaagg tgttaactt tttcgggcc caagtcgga 
      atttgtagt gagtgggtc agattgtgg ccacattta 
      tttctggga gcggaatag tgaagcgct agtgcagtg 
      gttacttgc cgatgataa tggagttgt taattgttt 
      ggagcctca cccccaatt accttttat ttgtacgag 
      tggtttgga ttgggacgt taaagtttg ggttccgtc 
      ctgtgaagt ggaaccaaa aatggaagg tttgagtga 
      cacaataaa agataattt aaaaatgaa actaggtca 
      tgaaggaac ggaatttaa cgaaaatcg gatgttgtg 
      gattctgtg gcgacatct caggaacac cactagact 
      aagggcgtc taatatgct taacattag agattttct 
      gcagcttta ttcagactc gaactcctc aggatcgga 
      gagcgttgc gaattctga cggttccga cgacgatct 
      tgataggcc accaaactg taatacttc ttaatcaac 
      gaaatttgt ttttgtagc atttatttt tattttgtt 
      a
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