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Gene information
General information
TC007643 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 1810392..1812881
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007643
    iBeetle screen iB_01234
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007643 RA
      MFSKSKLSV LPKTLRFSS SLCPSTAPI KPIVTLLDT 
      LSSVGSNLA LLLKQNLDI YELRLFDEE NNTNAFACD 
      LNEIDTRTK LKSFSCKSL KNAIVGAHV VISTGGCQE 
      KPGSSQREL FDKNLDNVR NVAMFLAEF NPEAIYCIA 
      KPPVEALVP MVSEEYKKA ETYDPRKII GVATVASMI 
      ANTFIAEHT NQNPADVLC PIIGGLSPK TTIPVLSQT 
      KPNVPFNPD LYKKLQEAV AHGEETLLC KGAMFCYSK 
      AVAISRFIH LVVKALKGE KHCVESAFV AQTGHIGEF 
      LPYMTSIVK LGPQGVAST HMPKINDLE TTRLRAAEP 
      HIVENILLG QTFIHGDFS IPPKFKVKR TCDALLKRK 
      VQECNNRAG MQYIN
    CDS sequence
    • >TC007643 RA
      atgttttca aagtcaaaa ttaagtgtc ctccctaaa 
      acccttcgt ttctcgtca tcgctgtgt ccctccaca 
      gcccccatc aaacccata gttaccctt ttagacacc 
      ttgagcagt gtgggttcc aaccttgcc cttctccta 
      aaacaaaac ctcgacatt tacgagtta cgtctcttc 
      gacgaagag aacaacact aatgccttc gcttgcgac 
      ttaaacgaa atagataca cgaacaaaa ctgaaatca 
      ttctcttgc aaatcgctg aaaaatgcc attgtgggg 
      gcacacgtg gtcatatcc accggcggc tgccaggaa 
      aaacctggc tcaagccaa agagagttg tttgataag 
      aacttggac aatgtgcga aatgttgca atgtttctg 
      gcggagttt aaccccgag gcgatttat tgcatcgcc 
      aagccgcca gttgaggct ttagtgccg atggtgtcc 
      gaggagtac aaaaaagca gagacctac gacccgaga 
      aaaatcatc ggtgtggca accgtagca tcaatgatt 
      gccaacact ttcatcgcc gaacacact aatcagaac 
      cctgccgac gtcctctgc cccataatt gggggcttg 
      agccccaaa accacaatt ccagtcctc tcacaaact 
      aagccaaac gtcccgttc aatccggac ttgtataaa 
      aaattgcaa gaggctgtc gcccacggc gaagagact 
      cttctgtgc aaaggcgca atgttttgc tactcgaaa 
      gcagtggcg atttcgcgt ttcattcat ttggttgta 
      aaggcccta aaaggggaa aaacactgc gtggaaagc 
      gcatttgtg gcccaaacc ggccacatt ggggagttt 
      ttgccgtac atgacctcc atagtcaaa ttaggcccc 
      caaggggtg gcgtccaca cacatgccc aaaataaac 
      gacttggag acgacgcga ttgagggcc gctgagccc 
      cacattgtt gaaaatatt cttctgggg caaacgttc 
      atccatggc gacttttca attcctccg aaatttaaa 
      gtaaagagg acttgtgac gcgcttttg aaacgcaaa 
      gtacaagaa tgtaacaat cgtgccgga atgcaatat 
      attaactaa
    mRNA sequence
    • >TC007643 RA
      gtagtaaca agacaaaat ttaaaaagt ttggaaatt 
      aacgttttg tctaaaaat atttgaggg aaaaaacaa 
      cttaggcaa tcaactctc cacgtttct cgtttcctc 
      gaacgttta aacatgtat ttgaattgt ttaaaaact 
      cagattcag attcaacca taaagagaa tttagtaaa 
      ttcggtcca gtcaaaaaa atgtcaaaa gtcgctccg 
      tcactcaaa atttccgct gaaaaatgt tttcaaagt 
      caaaattaa gtgtcctcc ctaaaaccc ttcgtttct 
      cgtcatcgc tgtgtccct ccacagccc ccatcaaac 
      ccatagtta cccttttag acaccttga gcagtgtgg 
      gttccaacc ttgcccttc tcctaaaac aaaacctcg 
      acatttacg agttacgtc tcttcgacg aagagaaca 
      acactaatg ccttcgctt gcgacttaa acgaaatag 
      atacacgaa caaaactga aatcattct cttgcaaat 
      cgctgaaaa atgccattg tgggggcac acgtggtca 
      tatccaccg gcggctgcc aggaaaaac ctggctcaa 
      gccaaagag agttgtttg ataagaact tggacaatg 
      tgcgaaatg ttgcaatgt ttctggcgg agtttaacc 
      ccgaggcga tttattgca tcgccaagc cgccagttg 
      aggctttag tgccgatgg tgtccgagg agtacaaaa 
      aagcagaga cctacgacc cgagaaaaa tcatcggtg 
      tggcaaccg tagcatcaa tgattgcca acactttca 
      tcgccgaac acactaatc agaaccctg ccgacgtcc 
      tctgcccca taattgggg gcttgagcc ccaaaacca 
      caattccag tcctctcac aaactaagc caaacgtcc 
      cgttcaatc cggacttgt ataaaaaat tgcaagagg 
      ctgtcgccc acggcgaag agactcttc tgtgcaaag 
      gcgcaatgt tttgctact cgaaagcag tggcgattt 
      cgcgtttca ttcatttgg ttgtaaagg ccctaaaag 
      gggaaaaac actgcgtgg aaagcgcat ttgtggccc 
      aaaccggcc acattgggg agtttttgc cgtacatga 
      cctccatag tcaaattag gcccccaag gggtggcgt 
      ccacacaca tgcccaaaa taaacgact tggagacga 
      cgcgattga gggccgctg agccccaca ttgttgaaa 
      atattcttc tggggcaaa cgttcatcc atggcgact 
      tttcaattc ctccgaaat ttaaagtaa agaggactt 
      gtgacgcgc ttttgaaac gcaaagtac aagaatgta 
      acaatcgtg ccggaatgc aatatatta actaaacaa 
      ttttgcacg gatttatgt ttaataacg atgggttcg 
      actgtatct gaaggtgct ggagactat tagggggct 
      ccccggacg ccccaaccg gtggccccc tttgccgaa 
      actactgtc tttcttcag gcacgattt taaggggta 
      ggagccccc tcgagtgta gtttttcga cgtcagttt 
      gcccggctt tttgtacag tctgataca cttaggttg 
      ctcgaaagt ttccttttc ttcgtgctg tttcttcaa 
      gttgagtat gaatgaatc cgtgtgttt caaaaactt 
      gttatcgtc caattcctc ctccgattt tttcgcaac 
      taaagtgtc ctgagtgtc cgtctctgc atcttccgt 
      caatttgga aatttccaa aacatcgtc agtttgtat 
      ttcctggca tttgctcgt aattttgat tttctcgac 
      ttgcgtttc gtcaagatg atcgccaaa ggatggata 
      aatttggat tttcttgcg cctgctttt aacaggggc 
      gctcttgat gacttgaat ttccaggga ttttttgct 
      gctcattga gtcgttctt gtccggctt ccagttggc 
      ccaatacgt ctcttccag tttttcaat caccgattt 
      cttcgactt ggtcttggg ttttttgcg gcttttcgg 
      cacgaaatc gccgtcgtt ttggtccgt cagactgac 
      gtaaatgtg gttgtagat ggggcatct tttgtgtct 
      ggaagtgtg ggcctggac tgagtagct atcactgtc 
      tgcggactg tttgaaatt ttggggctg gtagcggtc 
      caggtcgaa gtctggggg caaattttt gtgccgaaa 
      agataagtg gtgttcctg caaagaatg tgtaatgtg 
      ggcaggaca agggttgat aattacgca ttcctcggt 
      gtaaaattt cagggacct tgcattgtg taatttggg 
      acattcgaa cattgaata cgccattct ggacaatac 
      aacatttga aaattt