| General information | |||
| TC007640 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1817706..1820201 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007640 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01233 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 10 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC007640 RA
MSYSVNISV EAPIENQIQ EITYDGTEI YQPPLTLSE SLTKYAQKI DFSKTSDID FKKEPGEAI EDNKSDSDS KDAFQSSLW PWDSARNKL RNAFQEVCV LADVLAIAK DKRYMVLDP VQQEPIETK PMVQIYARK KALAGAASV LLTGAERLK TSQNEAARN RTVPDFHIE LLRLRQNWR LKKVSNTII GDLSYRTAG SKYTQTGTF EVTKAEDDD KTNSPPSSP IPGGATTAK TNSALRVSI PSELQGVAY IEVLCQKDQ EDLCSVNVN LLGSGPPAS NPDMHWQQK LEAAQNVLF CKELFNQLA KEAVQLQAP IPHMVVGNQ IMATVFPGI QLIIGLCHS TVGDKKQQN NNSSKTDHD HVLEHSLHQ LLREVHHKN THHPFPHPA TGPLGPSKK RMLAGPMAA DRYELLEMT KSETLLEQI IKQAQHFFM RLRTEYVLD TIAKEVKDP LISSHWNTL NSPTQSCVK INISTHGYD ALCRTPLVI HVGEKSLKC ICRDGKVMH MSYEPQELR DLIFCHINQ HQILAVQAL AKTMGWQFL ANSNHLGTG AVEPLGNAS SCLLASPTG DRIIAVRCE PQTGIQVCV AHSPRSDFF PSRLVTERK WEHLGGQFR DVRLEKMEG RNFLHKMEL LMASLTSSS
- >TC007640 RA
atgtcttac tcggtaaat atatcagtg gaagcgcct atagaaaac caaatacaa gaaatcacg tacgatgga acggaaata taccaacct ccgttgact ctatcagaa agtctcacc aaatacgcc caaaaaatc gactttagc aaaactagt gatattgac tttaaaaaa gaaccaggg gaggcaatt gaagacaat aaatctgac tctgacagt aaagacgct ttccagtca agtctatgg ccatgggac tcagccaga aacaaacta cgcaacgca tttcaggaa gtttgcgtc ttggctgac gtccttgcc atagctaag gacaaacgc tacatggtc ttggacccc gtccaacaa gaaccgatt gaaaccaaa ccaatggtg caaatatac gcccgtaag aaagcacta gctggggct gccagtgtc ctactcact ggtgccgaa cgactgaaa acgtcccaa aacgaagct gccagaaac agaactgtc ccagatttt cacatagaa ttgttaaga ttgcgccaa aattggagg cttaaaaaa gtctcaaat acaataatc ggggacttg agttatcgc acagctggc tctaaatac acacaaact ggcactttc gaagttacg aaagctgaa gacgatgat aagacaaac agtccgcct agtagtcca atacctgga ggtgccaca acggccaaa acaaattcc gctttgcga gtttcaatt ccttcagaa ttgcaaggg gtggcttat attgaagtt ttgtgtcag aaagatcaa gaagatctc tgtagtgtt aatgtaaat ttgttaggg tcaggtcca ccagcttca aaccctgac atgcactgg caacagaag ttagaagcg gcgcaaaat gttttgttc tgtaaagaa ttatttaac caacttgcc aaagaagca gttcagttg caggcgcct ataccacat atggttgta ggaaatcaa attatggcc acggttttt cccggcatt caattaata ataggattg tgtcactca actgtaggc gacaaaaaa caacaaaac aataactca tcaaaaacc gatcatgac cacgtattg gaacactca ttacaccag ttattgcga gaagtccac cacaaaaac acccatcac ccattcccc cacccagca acaggccca ttaggcccc tcaaagaaa cgaatgttg gccggacct atggccgca gacagatac gaattactc gaaatgaca aaatccgaa acacttctt gaacaaatc atcaaacaa gcgcaacac tttttcatg agattacgg acagaatac gtgcttgat acaatagct aaagaagtg aaagatccc cttataagc tcccactgg aacacttta aacagtccg acgcaatca tgcgttaaa atcaacatt tcaacacat ggttatgac gctctctgt cggacgcct ctcgtgata cacgtgggt gaaaaaagc ctcaagtgc atatgtaga gatgggaaa gtcatgcac atgagttac gagccgcaa gaattgcga gatttgatt ttttgccac ataaaccag caccaaatt ttggcggtt caagcgttg gctaagaca atgggttgg cagtttttg gccaattcg aaccatttg ggcacgggg gctgtggaa ccgctggga aacgctagt tcgtgtctt ttggcgtct cccacaggt gataggatt attgcagtt aggtgtgag ccacagacg ggtatccag gtctgtgtg gctcattcg cccaggagc gattttttc cccagtagg ttggtgact gagcgaaag tgggagcat ttaggcgga cagtttagg gatgttagg ttggaaaaa atggaaggc aggaatttc ttgcataag atggagttg ttaatggcg agtttgacg agtagctcg tag
- >TC007640 RA
cgtttttgt ggtaacctc aaacgtcaa atagcggta caaaaacaa ataatgtat tgttaattg ttatcttaa gtgtaaaaa aatgtctta ctcggtaaa tatatcagt ggaagcgcc tatagaaaa ccaaataca agaaatcac gtacgatgg aacggaaat ataccaacc tccgttgac tctatcaga aagtctcac caaatacgc ccaaaaaat cgactttag caaaactag tgatattga ctttaaaaa agaaccagg ggaggcaat tgaagacaa taaatctga ctctgacag taaagacgc tttccagtc aagtctatg gccatggga ctcagccag aaacaaact acgcaacgc atttcagga agtttgcgt cttggctga cgtccttgc catagctaa ggacaaacg ctacatggt cttggaccc cgtccaaca agaaccgat tgaaaccaa accaatggt gcaaatata cgcccgtaa gaaagcact agctggggc tgccagtgt cctactcac tggtgccga acgactgaa aacgtccca aaacgaagc tgccagaaa cagaactgt cccagattt tcacataga attgttaag attgcgcca aaattggag gcttaaaaa agtctcaaa tacaataat cggggactt gagttatcg cacagctgg ctctaaata cacacaaac tggcacttt cgaagttac gaaagctga agacgatga taagacaaa cagtccgcc tagtagtcc aatacctgg aggtgccac aacggccaa aacaaattc cgctttgcg agtttcaat tccttcaga attgcaagg ggtggctta tattgaagt tttgtgtca gaaagatca agaagatct ctgtagtgt taatgtaaa tttgttagg gtcaggtcc accagcttc aaaccctga catgcactg gcaacagaa gttagaagc ggcgcaaaa tgttttgtt ctgtaaaga attatttaa ccaacttgc caaagaagc agttcagtt gcaggcgcc tataccaca tatggttgt aggaaatca aattatggc cacggtttt tcccggcat tcaattaat aataggatt gtgtcactc aactgtagg cgacaaaaa acaacaaaa caataactc atcaaaaac cgatcatga ccacgtatt ggaacactc attacacca gttattgcg agaagtcca ccacaaaaa cacccatca cccattccc ccacccagc aacaggccc attaggccc ctcaaagaa acgaatgtt ggccggacc tatggccgc agacagata cgaattact cgaaatgac aaaatccga aacacttct tgaacaaat catcaaaca agcgcaaca ctttttcat gagattacg gacagaata cgtgcttga tacaatagc taaagaagt gaaagatcc ccttataag ctcccactg gaacacttt aaacagtcc gacgcaatc atgcgttaa aatcaacat ttcaacaca tggttatga cgctctctg tcggacgcc tctcgtgat acacgtggg tgaaaaaag cctcaagtg catatgtag agatgggaa agtcatgca catgagtta cgagccgca agaattgcg agatttgat tttttgcca cataaacca gcaccaaat tttggcggt tcaagcgtt ggctaagac aatgggttg gcagttttt ggccaattc gaaccattt gggcacggg ggctgtgga accgctggg aaacgctag ttcgtgtct tttggcgtc tcccacagg tgataggat tattgcagt taggtgtga gccacagac gggtatcca ggtctgtgt ggctcattc gcccaggag cgatttttt ccccagtag gttggtgac tgagcgaaa gtgggagca tttaggcgg acagtttag ggatgttag gttggaaaa aatggaagg caggaattt cttgcataa gatggagtt gttaatggc gagtttgac gagtagctc gtaggtttg tgaataata attttttgt aaaagttat aggaatcag ggccatgga agttgtttg tttgtcagt aggtacgtc ccgaggatt attacgaac ttttttaat ttcaacttg ttgaaaaaa agcagcttt ttattattc cttaggcca gatgcgtgt ttaaagtat tgtgtactt tttggaaat acataaatt acatttaat tgtcggcga tgcgaatgt gtattaaag ttgtattta ttttaagga aacccagtt tttgataaa tatgtgact aaattctct taaaaaat
