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Gene information
General information
TC007628 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 2013331..2019447
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007628
    iBeetle screen iB_01231
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment:
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: Cuticle almost not visible.
    larva cuticle mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Cuticle almost not visible.
    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment:
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007628 RA
      MNLSFAGCG FLGIYHVGV ACCFRKYAP HLLLNKISG 
      ASAGAIAAC CLLLDLPLG ETTSDILRV ATEARRRSL 
      GPFNPSFNI HSLLLEGLE KFLPDDAHI RVSGKLHIS 
      LTRVHDGKN VIVSQFDSR EELIQALLA TAFIPIFSG 
      IIPPKFKGV RYMDGGYSD NLPTLDENT ITVSPFCGE 
      SDICPRDDS SQLFHINIA NTSIELSKH NIYRIMRIL 
      FPPNPEVLS NMCKQGFDD ALRFLHRNN LINCTRCLA 
      VQSTFVVSD TLEENLEYD PQCKECKQH RQEALVSNM 
      PDTVLSIFQ EAIDSANNG LLNWVFKHR GMKLLSVLS 
      LPYTLPADM MYATFTKLM AAAPQVGNT VWDVSKYFM 
      DQLSHLLNK VNKKREQIS AKIICQLAV TEYGGRDVE 
      ACQDALSTK NKMNLNFTL NLDDSDLPL TQSPGRKFA 
      TTKILQRKP SLTINRTDT GDDDTFEHI LQVTSHHET 
      IMAYYYLDE NNKVKVTEI FDVTESDSP IVQSSHEKE 
      FNKSLEFDD DWDESTWTT THALDDQMY LDERRKSLA 
      EMSEYSLED LLDKDTSNL FSDPESEWT GTYRIEEPE 
      DEDEMPTAD LRPESDQPS YLRTTTIPS FTFHEES
    CDS sequence
    • >TC007628 RA
      atgaatctt tcgttcgct ggctgcggc ttcctgggc 
      atttaccac gtcggagtg gcttgctgc ttccgcaag 
      tatgccccc catctgctc ctcaacaaa atctcgggg 
      gcctcggcc ggggccatc gccgcctgc tgcctcctc 
      ctggacctg ccactaggt gaaacgacc agtgatatc 
      cttcgggtg gccacagag gcccggaga cggtctctg 
      ggccccttc aacccgtcc ttcaacata cacagcctt 
      ttgctggaa ggattagag aagttcctt cccgacgac 
      gctcatatc cgtgtcagt gggaagctc cacatttcg 
      ttgacacgt gtccacgac ggcaaaaac gtaatcgtt 
      tcgcagttc gacagtcgg gaggaactc atccaggcg 
      ctcctggcc acagccttc atcccgatt ttttccggg 
      attatccca cccaagttc aaaggagtg cgctatatg 
      gacgggggg tacagcgat aatttgccg actttggac 
      gaaaacaca attactgtc agccccttc tgtggggag 
      agtgatatc tgtcccaga gatgatagc tcgcagttg 
      tttcatatt aacatagct aatactagt atagagctg 
      tcgaagcac aatatttat aggattatg cggattttg 
      ttcccgcct aatccggaa gtcttgtca aatatgtgc 
      aagcagggg ttcgatgac gcgctccga ttcttgcac 
      aggaacaat ctgattaat tgcacgagg tgtctggct 
      gttcagtcg acgtttgtc gtttcggac actttggag 
      gagaacttg gagtacgac ccccaatgc aaggagtgc 
      aagcaacac agacaggaa gcgcttgtc tcaaacatg 
      cccgacacc gtcttgagc atcttccaa gaggcgatt 
      gattcagcc aacaacggt ctgcttaat tgggtattc 
      aaacaccgg ggaatgaaa ctgttatcc gttctaagt 
      cttccatac acactgcct gcagatatg atgtacgcc 
      acatttacc aagttgatg gctgcggct ccacaagtg 
      ggcaacacg gtctgggac gttagtaaa tatttcatg 
      gaccagctc tcacacctg ctcaataaa gtcaacaag 
      aagcgggaa caaatcagt gcgaaaata atttgccaa 
      ttggccgta accgaatac ggaggaaga gatgtcgaa 
      gcttgtcag gacgcgctg tccactaaa aataaaatg 
      aacttgaac tttactctc aatttagat gatagtgat 
      ctacctttg actcaaagt ccgggccgc aagttcgca 
      acgactaaa atactccaa cggaagcct agtctgaca 
      ataaaccgg accgatacc ggagatgac gacactttc 
      gagcacatt ttgcaagta acgtcgcac catgaaacc 
      ataatggct tactattat ttggacgaa aataataaa 
      gttaaagtc acggagatt ttcgacgtt acggaaagc 
      gactccccc atcgtgcaa tcctcccat gagaaggag 
      ttcaacaag agtttggag tttgacgat gattgggat 
      gagtctact tggaccacc actcacgct ctcgatgat 
      caaatgtat ctagacgag aggaggaag agtttggcc 
      gaaatgtcc gagtattcg cttgaagat cttctggat 
      aaggacacc tcgaacttg ttttcggac ccggaaagc 
      gagtggact ggcacttac aggattgaa gagccggaa 
      gatgaggat gagatgccc acagctgat ctgagaccg 
      gaatcagac caaccgagt tatttaagg accaccacg 
      ataccctct ttcacattc cacgaagag tcataa
    mRNA sequence
    • >TC007628 RA
      ccgtactaa ggtattttt aggggtttt tttgtctgc 
      aaagattct cgagaaaat ttcattgct ctcttagtt 
      gtaggacaa gtgcacaca cgttagaag acagtgaca 
      aagcgcgtg ataacgaat tagtgagat ttttatgtg 
      atatattgt gatagaaca atgaatctt tcgttcgct 
      ggctgcggc ttcctgggc atttaccac gtcggagtg 
      gcttgctgc ttccgcaag tatgccccc catctgctc 
      ctcaacaaa atctcgggg gcctcggcc ggggccatc 
      gccgcctgc tgcctcctc ctggacctg ccactaggt 
      gaaacgacc agtgatatc cttcgggtg gccacagag 
      gcccggaga cggtctctg ggccccttc aacccgtcc 
      ttcaacata cacagcctt ttgctggaa ggattagag 
      aagttcctt cccgacgac gctcatatc cgtgtcagt 
      gggaagctc cacatttcg ttgacacgt gtccacgac 
      ggcaaaaac gtaatcgtt tcgcagttc gacagtcgg 
      gaggaactc atccaggcg ctcctggcc acagccttc 
      atcccgatt ttttccggg attatccca cccaagttc 
      aaaggagtg cgctatatg gacgggggg tacagcgat 
      aatttgccg actttggac gaaaacaca attactgtc 
      agccccttc tgtggggag agtgatatc tgtcccaga 
      gatgatagc tcgcagttg tttcatatt aacatagct 
      aatactagt atagagctg tcgaagcac aatatttat 
      aggattatg cggattttg ttcccgcct aatccggaa 
      gtcttgtca aatatgtgc aagcagggg ttcgatgac 
      gcgctccga ttcttgcac aggaacaat ctgattaat 
      tgcacgagg tgtctggct gttcagtcg acgtttgtc 
      gtttcggac actttggag gagaacttg gagtacgac 
      ccccaatgc aaggagtgc aagcaacac agacaggaa 
      gcgcttgtc tcaaacatg cccgacacc gtcttgagc 
      atcttccaa gaggcgatt gattcagcc aacaacggt 
      ctgcttaat tgggtattc aaacaccgg ggaatgaaa 
      ctgttatcc gttctaagt cttccatac acactgcct 
      gcagatatg atgtacgcc acatttacc aagttgatg 
      gctgcggct ccacaagtg ggcaacacg gtctgggac 
      gttagtaaa tatttcatg gaccagctc tcacacctg 
      ctcaataaa gtcaacaag aagcgggaa caaatcagt 
      gcgaaaata atttgccaa ttggccgta accgaatac 
      ggaggaaga gatgtcgaa gcttgtcag gacgcgctg 
      tccactaaa aataaaatg aacttgaac tttactctc 
      aatttagat gatagtgat ctacctttg actcaaagt 
      ccgggccgc aagttcgca acgactaaa atactccaa 
      cggaagcct agtctgaca ataaaccgg accgatacc 
      ggagatgac gacactttc gagcacatt ttgcaagta 
      acgtcgcac catgaaacc ataatggct tactattat 
      ttggacgaa aataataaa gttaaagtc acggagatt 
      ttcgacgtt acggaaagc gactccccc atcgtgcaa 
      tcctcccat gagaaggag ttcaacaag agtttggag 
      tttgacgat gattgggat gagtctact tggaccacc 
      actcacgct ctcgatgat caaatgtat ctagacgag 
      aggaggaag agtttggcc gaaatgtcc gagtattcg 
      cttgaagat cttctggat aaggacacc tcgaacttg 
      ttttcggac ccggaaagc gagtggact ggcacttac 
      aggattgaa gagccggaa gatgaggat gagatgccc 
      acagctgat ctgagaccg gaatcagac caaccgagt 
      tatttaagg accaccacg ataccctct ttcacattc 
      cacgaagag tcataagtt aaaaatcgt cattttggt 
      gtaaccaaa attgtgata ttgtataaa aaaagatat 
      ttattaagt attatatga ccacattat ggctattta 
      tgataactt atgatagtt tttagttgt ataaaaatt 
      ataaagtat tgttattag ttcaaatta tcttttata 
      ttgttcgca ataaaaacg gttgtacta a