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Gene information
General information
TC007626 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 2096353..2097720
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007626
    iBeetle screen iB_01230
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Adult morphology
    hindwing irregular - Number of animals showing the phenotype: 5

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007626 RA
      MGNAGSGGA PRERPKYGE TVPSSPSRE GQAFVFEKK 
      PDKLLYQSS QDDEEPYFT RPVTADFDH QRPRANTVS 
      EGTKVNTSD KTPTVFRWE GGGKDVYVS GTFTEWKTI 
      PMVKSHGDF VTIIDLPEG EHQYKFYVD GEWKNDPGN 
      KMVEDESGV KNNLITVKK SDFEVFQAL DKDSENANN 
      DSQKEFSQE IPANKPWEK VTGPPILPP HLLQVILNK 
      DTPLSCEPT LLPEPNHVM LNHLYALSI KDGVMVLSA 
      THRYRKKYV TTLLYKPI
    CDS sequence
    • >TC007626 RA
      atgggcaat gcaggcagt ggcggagcg ccgcgggag 
      cgccccaag tatggcgag acggtgcca tcgtccccc 
      tccagagag ggccaagcg ttcgttttc gagaaaaaa 
      ccggacaag ttgctgtac cagagctcg caagacgac 
      gaagagccg tatttcacg cgccccgtg acggccgat 
      ttcgaccac cagagaccg cgagctaac actgtgtca 
      gagggtact aaagtcaac accagtgac aagacaccg 
      acggttttc cgatgggaa ggcgggggt aaggacgtc 
      tacgtcagt gggactttt acggagtgg aaaactatt 
      cctatggtg aaaagccac ggcgatttc gtaactatc 
      atcgacttg ccagaaggg gaacaccag tacaagttc 
      tacgttgat ggggagtgg aagaacgac cctgggaat 
      aaaatggtg gaggacgaa agcggtgtc aaaaataat 
      cttattacg gtgaaaaag tccgacttt gaggtgttt 
      caagccctg gataaggac agcgaaaat gccaataat 
      gactcgcag aaggagttc tcgcaggaa ataccggcc 
      aataagcca tgggagaag gtcacaggg ccccccata 
      ttgcccccg cacttactg caagttatt ctgaataag 
      gacacgcca ctttcgtgt gaaccaact ctcttacct 
      gaacccaac cacgtaatg ctaaaccac ctttacgcc 
      ttatccatc aaggatgga gtcatggta cttagcgcc 
      actcataga taccgcaag aaatacgtt actactctt 
      ttatataaa ccgatttaa
    mRNA sequence
    • >TC007626 RA
      aaggttgct ctcaaggtt aggcctgtc acgaacgtc 
      aaacttgta cacagtgta aataaattg ttagcgctt 
      taaatgttg gtaaactcg ggctcaagc gtgtaaaat 
      gggcaatgc aggcagtgg cggagcgcc gcgggagcg 
      ccccaagta tggcgagac ggtgccatc gtccccctc 
      cagagaggg ccaagcgtt cgttttcga gaaaaaacc 
      ggacaagtt gctgtacca gagctcgca agacgacga 
      agagccgta tttcacgcg ccccgtgac ggccgattt 
      cgaccacca gagaccgcg agctaacac tgtgtcaga 
      gggtactaa agtcaacac cagtgacaa gacaccgac 
      ggttttccg atgggaagg cgggggtaa ggacgtcta 
      cgtcagtgg gacttttac ggagtggaa aactattcc 
      tatggtgaa aagccacgg cgatttcgt aactatcat 
      cgacttgcc agaagggga acaccagta caagttcta 
      cgttgatgg ggagtggaa gaacgaccc tgggaataa 
      aatggtgga ggacgaaag cggtgtcaa aaataatct 
      tattacggt gaaaaagtc cgactttga ggtgtttca 
      agccctgga taaggacag cgaaaatgc caataatga 
      ctcgcagaa ggagttctc gcaggaaat accggccaa 
      taagccatg ggagaaggt cacagggcc ccccatatt 
      gcccccgca cttactgca agttattct gaataagga 
      cacgccact ttcgtgtga accaactct cttacctga 
      acccaacca cgtaatgct aaaccacct ttacgcctt 
      atccatcaa ggatggagt catggtact tagcgccac 
      tcatagata ccgcaagaa atacgttac tactctttt 
      atataaacc gatttaagc tattattta tattgttgt 
      gcataatcc tttaatgtt attgggttt cattgcttt 
      ctttaacag ctcctggaa caatgaaag aagttgctg 
      gtgggtcac agagcttca atgtggctc gttaccata 
      gttaccaaa ttaaattaa tgccttaat gtgggaaac 
      aaatgaatg cgatttttt tcattgcct taagacagc 
      tgtgctatg taaataaa