Status
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Gene information
General information
TC007619 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2254946..2260150
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007619
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01229

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    thoracic segment sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007619 RA
      MQIKNDISY CVRQKVPFA ADLPDLCAG 
      KAIYVTGVV LPKCSRFAV NLTCGKSPT 
      ADVAFHLNP RLLQHYIVR NTRLNGQWG 
      DEEKTSTVK FPFRREGKF NVCILIGDG 
      EFQVAVEGV FFCSYKFRV ALAKITAID 
      IQGCVDVLG VDCKQLQIY PEIGPGTVP 
      NVSVGHREA DTPKVGMDT PVTFLLPGG 
      FQRDWQLEI YGRVKILPH SFYVNLQEG 
      SHRWPHPRI PLHLNPRFS TY
    • >TC007619 RB
      MQIKNDISY CVRQKVPFA ADLPDLCAG 
      KAIYVTGVV LPKCSRFAV NLTCGKSPT 
      ADVAFHLNP RLLQHYIVR NTRLNGQWG 
      DEEKTSTVK FPFRREGKF NVCILIGDG 
      EFQVAVEGV FFCSYKFRV ALAKITAID 
      IQGCVDVLG VDCKQLQIY PEIGPGTVP 
      NVSVGHREA DTPKVGMVM GVAGNVFNC 
      VGLTWRGGF LGHS
    • >TC007619 RC
      MFLCAFQDT PVTFLLPGG FQRDWQLEI 
      YGRVKILPH SFYVNLQEG SHRWPHPRI 
      PLHLNPRFS MGSGEPLFV RNAWIDGGW 
      GQEERTPGF QFAPGSPFT MAIRRGSDH 
      FSVWIDGKL AGEFKFRGT VDNTDTVHM 
      EGDVVIFDV RLNQPL
    CDS sequence
    • >TC007619 RA
      atgcaaatt aaaaatgac atctcttat tgtgttcga 
      cagaaagtg ccttttgcg gccgacctt ccggattta 
      tgcgccgga aaagcgatt tatgtcacc ggagtcgtt 
      ttgccgaaa tgctcaaga ttcgcggtg aatttaaca 
      tgcggcaaa agtccaaca gcggacgtg gcgttccat 
      ctgaatccg cgcctcctc cagcactac atcgtccga 
      aacactcga ctaaacggc caatgggga gacgaggaa 
      aaaacctcc acagtgaag ttccccttt cgacgcgaa 
      ggaaaattc aacgtttgc attcttatc ggcgacggg 
      gagtttcaa gtggccgtg gaaggggtg tttttttgc 
      agctacaag ttccgggtg gccttagcc aaaatcact 
      gcaatcgac atacagggc tgtgttgat gtcttggga 
      gtggattgc aagcaactg caaatttat cccgaaatc 
      ggacccgga accgtgcct aacgtctcc gtgggacat 
      cgggaagcc gatactccc aaagtcgga atggatact 
      ccggttacg tttttattg cctggaggt tttcagcga 
      gactggcaa ttagagatt tacggccgg gtaaaaatt 
      ctaccccat tcgttctac gttaatcta caagagggg 
      tcgcatcga tggccgcat cccaggatc ccgctgcat 
      ttgaatccg cgctttagt acgtattga
    • >TC007619 RB
      atgcaaatt aaaaatgac atctcttat tgtgttcga 
      cagaaagtg ccttttgcg gccgacctt ccggattta 
      tgcgccgga aaagcgatt tatgtcacc ggagtcgtt 
      ttgccgaaa tgctcaaga ttcgcggtg aatttaaca 
      tgcggcaaa agtccaaca gcggacgtg gcgttccat 
      ctgaatccg cgcctcctc cagcactac atcgtccga 
      aacactcga ctaaacggc caatgggga gacgaggaa 
      aaaacctcc acagtgaag ttccccttt cgacgcgaa 
      ggaaaattc aacgtttgc attcttatc ggcgacggg 
      gagtttcaa gtggccgtg gaaggggtg tttttttgc 
      agctacaag ttccgggtg gccttagcc aaaatcact 
      gcaatcgac atacagggc tgtgttgat gtcttggga 
      gtggattgc aagcaactg caaatttat cccgaaatc 
      ggacccgga accgtgcct aacgtctcc gtgggacat 
      cgggaagcc gatactccc aaagtcgga atggtaatg 
      ggagttgcg ggaaatgtt tttaattgc gtggggttg 
      acttggagg ggaggtttt ctcgggcat tcttaa
    • >TC007619 RC
      atgttttta tgtgctttt caggatact ccggttacg 
      tttttattg cctggaggt tttcagcga gactggcaa 
      ttagagatt tacggccgg gtaaaaatt ctaccccat 
      tcgttctac gttaatcta caagagggg tcgcatcga 
      tggccgcat cccaggatc ccgctgcat ttgaatccg 
      cgctttagt atgggttcg ggggagccc ttgttcgtg 
      aggaacgcc tggattgac ggaggttgg ggtcaagaa 
      gagcgcact cctggattc caatttgcg cccggctca 
      cctttcact atggcaatc cgacgaggg agcgatcat 
      ttttctgtt tggatagac ggcaaacta gccggagaa 
      tttaaattt aggggaacc gtcgacaac actgatacc 
      gtccacatg gaaggggac gtcgttatc tttgatgtg 
      cgtttaaac cagccgctc taa
    mRNA sequence
    • >TC007619 RA
      cccatgtgg aaaaatatc gagctcatt tatttataa 
      ctattacct tcagtcgaa accgcgctt tcaacacgc 
      ctagcgtga aaaatgtcg gtcgagacc gaaaaacaa 
      gtgaagtgc tgctgcttt tcgaaaaat tcgaaacag 
      gtcgagatg gcctcgcaa aacaaaacc gaatcgtcg 
      ctcccaacg gcggtaaat ttgatttta gaaactttt 
      ggtgctggt tttgaccgc actgctggt cgataccca 
      ggtagaaag tgctaaatt ctaatttct tttgaggac 
      gcactcgcc cgataagcc tgacatgtc attccagct 
      gtgataact gcttagtga ttttttgaa ttcaattag 
      tacaatgca aattaaaaa tgacatctc ttattgtgt 
      tcgacagaa agtgccttt tgcggccga ccttccgga 
      tttatgcgc cggaaaagc gatttatgt caccggagt 
      cgttttgcc gaaatgctc aagattcgc ggtgaattt 
      aacatgcgg caaaagtcc aacagcgga cgtggcgtt 
      ccatctgaa tccgcgcct cctccagca ctacatcgt 
      ccgaaacac tcgactaaa cggccaatg gggagacga 
      ggaaaaaac ctccacagt gaagttccc ctttcgacg 
      cgaaggaaa attcaacgt ttgcattct tatcggcga 
      cggggagtt tcaagtggc cgtggaagg ggtgttttt 
      ttgcagcta caagttccg ggtggcctt agccaaaat 
      cactgcaat cgacataca gggctgtgt tgatgtctt 
      gggagtgga ttgcaagca actgcaaat ttatcccga 
      aatcggacc cggaaccgt gcctaacgt ctccgtggg 
      acatcggga agccgatac tcccaaagt cggaatgga 
      tactccggt tacgttttt attgcctgg aggttttca 
      gcgagactg gcaattaga gatttacgg ccgggtaaa 
      aattctacc ccattcgtt ctacgttaa tctacaaga 
      ggggtcgca tcgatggcc gcatcccag gatcccgct 
      gcatttgaa tccgcgctt tagtacgta ttgatgaat 
      tttcaagta aaaaaattc cgccgggtt atccgattt 
      agtccggtt ctaattaga cgacctagc catcagtgt 
      agtgtgttg cgatgcatt attaattga tttaatttg 
      gtacaaagc tcaaacaac tcgcactgt aacagcgtt 
      ataacgtga tttatttaa ttggataag ggaacaacg 
      ctgctaatt tgcttaatt tttcatggg ggcaggtag 
      gtggttgct gaaaggcat cgcaattat gcttaatgt 
      gcgttatct aatcgggaa aaaaccgac gggatattc 
      caggtatgg gttcggggg agcccttgt tcgtgagga 
      acgcctgga ttgacggag gttggggtc aagaagagc 
      gcactcctg gattccaat ttgcgcccg gctcacctt 
      tcactatgg caatccgac gagggagcg atcattttt 
      ctgtttgga tagacggca aactagccg gagaattta 
      aatttaggg gaaccgtcg acaacactg ataccgtcc 
      acatggaag gggacgtcg ttatctttg atgtgcgtt 
      taaaccagc cgctctaat taaaacaca tagttttat 
      attcaatta aatcgactg caattatta cccttacgg 
      atgagtgat caaagagcg gccgtcaaa tgtggccat 
      ttaataagc atatattaa tatataaat gataatcag 
      ccagaaaga aactgtaaa tattgtgtt ttatatgag 
      atgagcaaa taaaatgca tgaaaacta
    • >TC007619 RB
      cccatgtgg aaaaatatc gagctcatt tatttataa 
      ctattacct tcagtcgaa accgcgctt tcaacacgc 
      ctagcgtga aaaatgtcg gtcgagacc gaaaaacaa 
      gtgaagtgc tgctgcttt tcgaaaaat tcgaaacag 
      gtcgagatg gcctcgcaa aacaaaacc gaatcgtcg 
      ctcccaacg gcggtaaat ttgatttta gaaactttt 
      ggtgctggt tttgaccgc actgctggt cgataccca 
      ggtagaaag tgctaaatt ctaatttct tttgaggac 
      gcactcgcc cgataagcc tgacatgtc attccagct 
      gtgataact gcttagtga ttttttgaa ttcaattag 
      tacaatgca aattaaaaa tgacatctc ttattgtgt 
      tcgacagaa agtgccttt tgcggccga ccttccgga 
      tttatgcgc cggaaaagc gatttatgt caccggagt 
      cgttttgcc gaaatgctc aagattcgc ggtgaattt 
      aacatgcgg caaaagtcc aacagcgga cgtggcgtt 
      ccatctgaa tccgcgcct cctccagca ctacatcgt 
      ccgaaacac tcgactaaa cggccaatg gggagacga 
      ggaaaaaac ctccacagt gaagttccc ctttcgacg 
      cgaaggaaa attcaacgt ttgcattct tatcggcga 
      cggggagtt tcaagtggc cgtggaagg ggtgttttt 
      ttgcagcta caagttccg ggtggcctt agccaaaat 
      cactgcaat cgacataca gggctgtgt tgatgtctt 
      gggagtgga ttgcaagca actgcaaat ttatcccga 
      aatcggacc cggaaccgt gcctaacgt ctccgtggg 
      acatcggga agccgatac tcccaaagt cggaatggt 
      aatgggagt tgcgggaaa tgtttttaa ttgcgtggg 
      gttgacttg gaggggagg ttttctcgg gcattctta 
      acgcacttt actgtattt atctaactc cccacatgt 
      aggcagcac taattgcca attacatcc ctcacgggc 
      gatcaatac gacaaaaac acggctatt tctttacag 
      ggtttgcct cagaagaca attttttcc agtttcatt 
      cacaggttc agctttttt atgtttttg cgaggcgtt 
      aaaatttaa attctataa agatttaaa aaaaattcc 
      atcacaggt caataaaaa aataaagct tcaacaaag 
      ta
    • >TC007619 RC
      atccttgtc tgtacaatg tcggattaa aagtgcgct 
      ctgaatatt aaattatca aaatattaa acaatcgcg 
      gtgtattat ctttttaga acgtctggt agttgaagt 
      ttgcagttt agcaaaact taaaataat gttttaata 
      aaaaaagtt gtgaagttt gtgggggat aactcgcgt 
      tgatgcact ttgtggagc atcaaaata gcaacagct 
      tagttttaa taatgaagg agtttggcc ttttgtctt 
      gccgatcct ttcgaatat ttgtcttgt gcgcgttgc 
      tgtaaaaac agtgggaga taattaaat tttacatga 
      tttttcagg tgatgtttt tatgtgctt ttcaggata 
      ctccggtta cgtttttat tgcctggag gttttcagc 
      gagactggc aattagaga tttacggcc gggtaaaaa 
      ttctacccc attcgttct acgttaatc tacaagagg 
      ggtcgcatc gatggccgc atcccagga tcccgctgc 
      atttgaatc cgcgcttta gtatgggtt cgggggagc 
      ccttgttcg tgaggaacg cctggattg acggaggtt 
      ggggtcaag aagagcgca ctcctggat tccaatttg 
      cgcccggct cacctttca ctatggcaa tccgacgag 
      ggagcgatc atttttctg tttggatag acggcaaac 
      tagccggag aatttaaat ttaggggaa ccgtcgaca 
      acactgata ccgtccaca tggaagggg acgtcgtta 
      tctttgatg tgcgtttaa accagccgc tctaattaa 
      aacacatag ttttatatt caattaaat cgactgcaa 
      ttattaccc ttacggatg agtgatcaa agagcggcc 
      gtcaaatgt ggccattta ataagcata tattaatat 
      ataaatgat aatcagcca gaaagaaac tgtaaatat 
      tgtgtttta tatgagatg agcaaataa aatgcatga 
      aaacta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY