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Gene information
General information
TC007603 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2490273..2491480
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007603
      iBeetle screen iB_01228

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
      embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007603 RA
        MVNEDVAVP EETEFTLKY NSWYAITIS TIIWAASYQ 
        LLRATLPSK NREYCCRVL SFLHGIITA FVGINQCFL 
        IDTPFEHPE WRTTNSQRF LMVCSLGYF IHDLVWCFV 
        YQRDSKLML AHHLYSVCA LRRMLYKNN SGAQATCAL 
        GSMEISNPM LQIRWFLRS EGYYPSNLY TSVEITFMI 
        IFFLVRIVL GTYFLIVIA FQPKNDWDF RVLAVTIYA 
        MSWMFMINI TKYFVTKYG LGYKGNDIQ NKGT
      CDS sequence
      • >TC007603 RA
        atggtaaat gaggatgtc gccgtaccg gaagaaact 
        gaattcacc ctaaaatac aactcctgg tacgcgatt 
        actataagc acaatcata tgggcggcc agctaccag 
        cttctgagg gccacgttg ccgtcaaaa aatcgcgag 
        tactgctgc cgggttttg agttttttg cacggaatc 
        atcaccgct tttgtgggg ataaaccag tgttttctc 
        atcgacact ccgtttgag cacccggaa tggcggacg 
        accaacagc caacgattt ttgatggtg tgcagcttg 
        gggtatttt atccacgac ttggtttgg tgctttgtg 
        taccagagg gactcgaaa ttgatgctc gctcatcat 
        ctttacagc gtgtgtgcg ctgagaaga atgctgtat 
        aagaacaac tcgggggcg caagccact tgtgccttg 
        ggaagtatg gaaatttcg aatccgatg ttgcagatt 
        cgatggttc ctcagaagc gaaggatat tatccttca 
        aacttgtac acgtcggtc gagatcacg ttcatgatc 
        atatttttc ctagtgcgc attgtcctc gggacgtat 
        tttttaatt gtgattgcc tttcaaccg aaaaacgac 
        tgggacttt agagtcctt gccgtgacg atttatgca 
        atgtcttgg atgtttatg ataaatatc accaagtat 
        ttcgttacc aaatatgga cttgggtat aaaggaaac 
        gatattcag aataaggga acttga
      mRNA sequence
      • >TC007603 RA
        cgtttttgt aatgatgag gaaaatagt tatttttgc 
        agtccagtg tgtcactga tggaaaatc tggtaataa 
        aaagtaatt acgagctgg tagttttat tgcgaattt 
        cggtaatac acaaaacca ggataagct taattgtaa 
        ctaatgtag ggcttgatt gagtgaatg gtgccgcaa 
        gggcatata tcgagcagt cctaaaata tcccaaaaa 
        ttcaccgaa atactgtac agtttggcg aaaactttg 
        tttttgcgg ctgcgtgtc ttatccatc ttgtcaaaa 
        gtgtcctcc ttttctgct ctcttcttg aaagaacac 
        gaacttgag ctcgaatta cttgacata atctttgac 
        aattttgtt ttgacaaac atgccacta aaattcttt 
        gtgttttcc tcaaaaaat ggtaaatga ggatgtcgc 
        cgtaccgga agaaactga attcaccct aaaatacaa 
        ctcctggta cgcgattac tataagcac aatcatatg 
        ggcggccag ctaccagct tctgagggc cacgttgcc 
        gtcaaaaaa tcgcgagta ctgctgccg ggttttgag 
        ttttttgca cggaatcat caccgcttt tgtggggat 
        aaaccagtg ttttctcat cgacactcc gtttgagca 
        cccggaatg gcggacgac caacagcca acgattttt 
        gatggtgtg cagcttggg gtattttat ccacgactt 
        ggtttggtg ctttgtgta ccagaggga ctcgaaatt 
        gatgctcgc tcatcatct ttacagcgt gtgtgcgct 
        gagaagaat gctgtataa gaacaactc gggggcgca 
        agccacttg tgccttggg aagtatgga aatttcgaa 
        tccgatgtt gcagattcg atggttcct cagaagcga 
        aggatatta tccttcaaa cttgtacac gtcggtcga 
        gatcacgtt catgatcat atttttcct agtgcgcat 
        tgtcctcgg gacgtattt tttaattgt gattgcctt 
        tcaaccgaa aaacgactg ggactttag agtccttgc 
        cgtgacgat ttatgcaat gtcttggat gtttatgat 
        aaatatcac caagtattt cgttaccaa atatggact 
        tgggtataa aggaaacga tattcagaa taagggaac 
        ttgactgcc ggcttattt gttcatttt tatgtgtta 
        aataaatta tttaagtgg aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY