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Gene information
General information
TC007593 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 2636001..2650571
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007593
    iBeetle screen iB_01227
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007593 RA
      MVLKYCDHL HGKWYFSEV RAIFSRKYL LQNVAIEIF 
      LASRTSIMF AFPDQATVK KVIKALPRV GVGIKYGIP 
      QTRRASMMS PRQLMRNSN MTQKWQRRE ISNFEYLMF 
      LNTIAGRTY NDLNQYPVF PWVLTNYET KELDLSQPS 
      NYRDLSKPI GALNPNRRA YFEERYSTW EHESIPPFH 
      YGTHYSTAA FVYNWMIRV EPFTTMFLA LQGGKFDYP 
      NRLFTSVSL SWKNCQRDT SDVKELIPE FFFLPEMFV 
      NANRYRLGI GEDGKAIGD VELPPWANS PEEFVRINR 
      MALESEFVS CQLHQWIDL VFGYKQRGP EAVRATNVF 
      YYLTYESNV ELDSIQDKV MREAVENQI RNFGQTPSQ 
      LLMEPHPPR SSAMHLSPM MFSSIPDDV CMTMKFLSN 
      SPIVHISAN TYPQLSNPS VVTVTMHQQ FAVNRWNSG 
      YAAVAQSPS YAETPQNQA ANLPLSMDP VLSQTNNSA 
      QNHMLRRHL GDNFSQNLK IRSNCFVTT VDSKFLIAC 
      GFWDNSFRV FSTETAKIV QIIFGHYGV VTCLSRSEC 
      NITSDCYIA SGSVDCTVL LWHWNARTQ TIVGEGEIP 
      TPRATLTGH EQPVSSVVI SAELGLVVS GSYNGPVLV 
      HTTFGDLLR SLEPPQGYA SPENIAMSR EGVIVVNYE 
      KGNIAAFTI NGKRLRHES HNDNLQCLL LSRDGEYLM 
      TGGDKGIVE VWRTFNLAL LYAFPACDS SVRSLALSH 
      DQKFLLAGL ALGSIVVFH IDFNRWHHE FQQRY
    CDS sequence
    • >TC007593 RA
      atggtcctg aaatactgc gaccacttg cacggaaag 
      tggtacttt tccgaagtc agggccatc ttctcgcgg 
      aaatacctg ctccagaat gtggccatc gagattttc 
      ctcgccagc agaacttcc atcatgttt gcgtttccc 
      gatcaagcc accgtgaaa aaggtaata aaggccttg 
      cctcgggtg ggtgtgggt atcaaatat ggaatacca 
      caaacaaga cgggctagt atgatgtca ccgcgacaa 
      ctgatgagg aattcaaat atgacgcag aaatggcaa 
      cgaagggag atatcaaat ttcgaatat ctcatgttt 
      ctgaatacg atagccggt cgaacctac aacgacctc 
      aaccaatac cccgtattt ccgtgggtc ctcaccaac 
      tacgagacc aaagaactc gacctaagc caacctagc 
      aactatcgt gacctttct aaacccatc ggagctctg 
      aatccgaac cgacgagcc tatttcgag gagagatac 
      tcgacatgg gagcacgaa agcatccca ccgttccat 
      tacggcact cactactcg actgccgct ttcgtctac 
      aactggatg attcgtgtc gagcccttc accacaatg 
      ttcctcgcc ttgcaaggc ggcaaattc gactacccc 
      aaccgactg ttcacttcg gtgtcgctt tcatggaag 
      aactgtcag cgcgatact tccgacgtc aaggagtta 
      atcccggag tttttcttc ctgccggag atgttcgtt 
      aatgcgaat cgatacagg ttggggatc ggcgaggac 
      gggaaggct atcggcgat gtggagcta ccaccatgg 
      gcgaactcc ccggaggag ttcgtccgg attaaccga 
      atggcgctc gagtcggag ttcgtgtct tgtcagctg 
      caccaatgg atcgatttg gtgttcggc tacaagcag 
      cgaggccca gaggccgtg cgagcgacc aatgttttt 
      tactatttg acgtatgag agcaacgtt gagttggac 
      tcgattcag gataaggtg atgagggaa gcagtggag 
      aaccagatc cgcaatttc gggcaaaca ccgtcgcag 
      ttgttgatg gaaccgcac cctccgcgg agctcagcc 
      atgcatttg tcgcccatg atgttctcg agtattccc 
      gatgatgtg tgcatgacc atgaagttc ctgtcgaat 
      agtcccatc gtccacatt tcggccaac acttacccg 
      cagttgtcc aacccatcg gtggtcaca gtgaccatg 
      caccagcaa ttcgccgtc aatcggtgg aactctggg 
      tacgcagct gtggcacag tcacccagt tatgcggaa 
      acgccgcaa aatcaagct gccaattta ccactgtcc 
      atggaccca gttttatcc caaacgaat aacagtgcc 
      caaaatcac atgctccgc cgccacttg ggcgataat 
      ttcagtcag aatttgaaa attcggtct aactgtttc 
      gtcacgacc gttgatagt aaatttttg attgcttgc 
      ggtttttgg gataatagt tttcgggtg ttttcgacc 
      gagactgcc aaaatcgtg cagattatt ttcggtcat 
      tacggcgtt gtcacttgt ttgtcacga tcggagtgt 
      aatataact tccgattgt tatattgca tcgggaagt 
      gtcgactgt accgttttg ttgtggcac tggaacgca 
      cgaactcag acgattgtg ggcgaaggg gaaataccg 
      actcctaga gcgactctg acggggcac gaacagccc 
      gtttccagt gtggtgatc tcggccgag ctcggattg 
      gtcgtttcg gggtcttac aatggtcct gtgttggtc 
      cacacgacc ttcggcgat ctgctccgg tctttggag 
      ccccctcag ggctacgcc tcgccggag aacatcgcg 
      atgtcgcgc gagggcgtc atcgtcgtc aactacgaa 
      aaggggaat attgccgca tttaccatc aacgggaaa 
      cggctgaga cacgagtcc cataacgat aatttacag 
      tgcctatta cttagccga gatggtgaa tatttgatg 
      accggtgga gacaaaggc attgtggaa gtgtggaga 
      acgttcaac ttggcttta ttatatgcc ttcccagcg 
      tgtgatagt agcgttagg tctcttgcg ttatcacat 
      gatcaaaag tttttgcta gcgggtcta gctctagga 
      tcgatagtc gtatttcac attgatttc aatagatgg 
      caccatgaa tttcagcaa cgttactaa
    mRNA sequence
    • >TC007593 RA
      aatccatcc acctggatg cacctttgg gggattagt 
      ggtcgcgcg tcacccaca tggtcctga aatactgcg 
      accacttgc acggaaagt ggtactttt ccgaagtca 
      gggccatct tctcgcgga aatacctgc tccagaatg 
      tggccatcg agattttcc tcgccagca gaacttcca 
      tcatgtttg cgtttcccg atcaagcca ccgtgaaaa 
      aggtaataa aggccttgc ctcgggtgg gtgtgggta 
      tcaaatatg gaataccac aaacaagac gggctagta 
      tgatgtcac cgcgacaac tgatgagga attcaaata 
      tgacgcaga aatggcaac gaagggaga tatcaaatt 
      tcgaatatc tcatgtttc tgaatacga tagccggtc 
      gaacctaca acgacctca accaatacc ccgtatttc 
      cgtgggtcc tcaccaact acgagacca aagaactcg 
      acctaagcc aacctagca actatcgtg acctttcta 
      aacccatcg gagctctga atccgaacc gacgagcct 
      atttcgagg agagatact cgacatggg agcacgaaa 
      gcatcccac cgttccatt acggcactc actactcga 
      ctgccgctt tcgtctaca actggatga ttcgtgtcg 
      agcccttca ccacaatgt tcctcgcct tgcaaggcg 
      gcaaattcg actacccca accgactgt tcacttcgg 
      tgtcgcttt catggaaga actgtcagc gcgatactt 
      ccgacgtca aggagttaa tcccggagt ttttcttcc 
      tgccggaga tgttcgtta atgcgaatc gatacaggt 
      tggggatcg gcgaggacg ggaaggcta tcggcgatg 
      tggagctac caccatggg cgaactccc cggaggagt 
      tcgtccgga ttaaccgaa tggcgctcg agtcggagt 
      tcgtgtctt gtcagctgc accaatgga tcgatttgg 
      tgttcggct acaagcagc gaggcccag aggccgtgc 
      gagcgacca atgtttttt actatttga cgtatgaga 
      gcaacgttg agttggact cgattcagg ataaggtga 
      tgagggaag cagtggaga accagatcc gcaatttcg 
      ggcaaacac cgtcgcagt tgttgatgg aaccgcacc 
      ctccgcgga gctcagcca tgcatttgt cgcccatga 
      tgttctcga gtattcccg atgatgtgt gcatgacca 
      tgaagttcc tgtcgaata gtcccatcg tccacattt 
      cggccaaca cttacccgc agttgtcca acccatcgg 
      tggtcacag tgaccatgc accagcaat tcgccgtca 
      atcggtgga actctgggt acgcagctg tggcacagt 
      cacccagtt atgcggaaa cgccgcaaa atcaagctg 
      ccaatttac cactgtcca tggacccag ttttatccc 
      aaacgaata acagtgccc aaaatcaca tgctccgcc 
      gccacttgg gcgataatt tcagtcaga atttgaaaa 
      ttcggtcta actgtttcg tcacgaccg ttgatagta 
      aatttttga ttgcttgcg gtttttggg ataatagtt 
      ttcgggtgt tttcgaccg agactgcca aaatcgtgc 
      agattattt tcggtcatt acggcgttg tcacttgtt 
      tgtcacgat cggagtgta atataactt ccgattgtt 
      atattgcat cgggaagtg tcgactgta ccgttttgt 
      tgtggcact ggaacgcac gaactcaga cgattgtgg 
      gcgaagggg aaataccga ctcctagag cgactctga 
      cggggcacg aacagcccg tttccagtg tggtgatct 
      cggccgagc tcggattgg tcgtttcgg ggtcttaca 
      atggtcctg tgttggtcc acacgacct tcggcgatc 
      tgctccggt ctttggagc cccctcagg gctacgcct 
      cgccggaga acatcgcga tgtcgcgcg agggcgtca 
      tcgtcgtca actacgaaa aggggaata ttgccgcat 
      ttaccatca acgggaaac ggctgagac acgagtccc 
      ataacgata atttacagt gcctattac ttagccgag 
      atggtgaat atttgatga ccggtggag acaaaggca 
      ttgtggaag tgtggagaa cgttcaact tggctttat 
      tatatgcct tcccagcgt gtgatagta gcgttaggt 
      ctcttgcgt tatcacatg atcaaaagt ttttgctag 
      cgggtctag ctctaggat cgatagtcg tatttcaca 
      ttgatttca atagatggc accatgaat ttcagcaac 
      gttactaaa cttagcacg tacttattc ctgttttaa 
      tgtagtact taatattgg attttattg ttcaattac 
      agtagcgtt tcgtttgtt tttggatat tttaatgtg 
      caatatctt cgtagtttt ttattgtta aatgatttc 
      gttttatgt atagatgta gataatggt tttacattc 
      cgtagtaag ttagtattg gcttcgtct gttgtaaaa 
      tactaactt atgaagata gtgtgcgtt attatatga 
      tttgattcg agagaaact agcttttaa gggttaaaa 
      cagttcaag tcaatcttt aaagctggc ttttaatca 
      gttgtgagt gagttaagc acatagtta tgcttaggg 
      cttgcacat ccattgcca gcaattgtt gagccagat 
      ttggcgcac tttttcgat actgtatct tcatcaacg 
      cccgaggtc ggtaaagat acagtcgtc ttaaaaacc 
      attccctcc agcatatgt attatatat tgcaaatct 
      gtccagata taaaaatca aacttattg tatataagt 
      aacttgttt ttttatgat acgctactt aattatgtg 
      tatttaaaa gtatttaat tcttgtatt attttatta 
      cttatggag attgtactt aattgtaat aaggaacat 
      tgtatgttt gatgttagc aataaacat tcatgattt 
      gt