General information | |||
TC007589 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 2753907..2756902 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC007589 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01226 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 7 (11 dpi)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (16 dpi)
remarks: later than schedule, hours 96
- >TC007589 RA
MEMHIETAV RIHTSGYSP NDMVCVHSN PVNNTIQLA NSQNYPVNY ALPADCCQN TVFTTTAAP LVNYLVEGC DVSVVTLGQ SGTGKSYTL LGPGFHCAA SEADHGIIP RFIREVFAK MKQYRDRNW CVHIAWSQI CGENVQDLL GVGSIECRN ISDVFQLIQ VGMSNIAPK CAHTLFTLT LEQQWVVET SVQHRVSTA SFADLAGTE KVLVYDSNG MMQTIPTDL GIQTLQRCI MSLSDFNCS FVPYTQSVL TTLLKDSFG GRAKTLLIC CVSPLAQDF TETLYTLQL GLRAQMIKN FVTVNSYAT FESTQENCD VFGLQFAAN QLLKLVSNA EELFQRLVA NDLLPKSEQ EQISQWLML KQECEDCLS ENSEPHRSL ERIEEEIES DSSESEAIE EEEKESLLD RVQGLMETF RSNTDKLVS EANCANVTN SCTKESMNS SNNEYHMKG ARGRRGSIH SAEELNPCL SLNTLRIND DVESQKIAV TSYDNKKKI LKQIATALE LNQKKISDL EKTIRVKEN LMEKLLKHK DTKSSAHHK IEQKCQLLR KEYKNAEVK FLQAKLQKN HHLEGKYKS EVVALEERL RDTESLKNL TEDGNKLLE LESSLHTDR EKLKKYKQE KEKYKQIYE KQLKEERSK SKASCESVP KTPDVKVVA LIKPNVSLN SEELESLRH EIRNLRKTR DYLLELRCK ITLKSQNKK FLNDSDERK LLQYEEAIE AIDLIIEYK NSILCGHLP VKEIEDHGD RMLMERFLK LSENEMRIL LHRYFEKII DLRSSSKKL DLEILDIEN QHENLAYRV QKLSHNLQL VRSDAERRI ASLQQQHED KLYLVLRNL TGDGADNER ISRVLGSRH SAVTVQVAG ASKQVDKNR FIAKWTRYR QETVPRQLQ SVPPQAKVT IEKNKIILQ KTNKN
- >TC007589 RA
atggagatg cacatagaa acggcggtc cgcatccat acgtcgggc tactccccc aacgacatg gtttgcgtg cactccaac cccgtcaac aacaccatc caactcgcg aattcgcaa aactacccc gtcaattac gcccttcca gccgactgt tgccaaaac acggtgttc acaaccacg gcagcccct ttggtgaat tatttggtg gaagggtgc gatgtttcg gtggtcacg ctgggccag tccgggact ggaaagtcc tacacgctc ctaggccca ggtttccac tgtgcggct agcgaggct gatcacggg attatcccg cggtttatt cgcgaagtt ttcgccaaa atgaaacaa tacagagac aggaattgg tgcgtgcat atcgcttgg tcgcagatt tgtggcgaa aacgtccag gatttgcta ggcgttggg agcatcgag tgccggaat atcagtgac gtgtttcag ttgattcaa gtcggaatg tcgaatata gcccccaag tgcgcccac acgttgttc acactcact ttggagcag cagtgggtg gtggagaca agtgtgcaa catcgcgtc tcaactgcg agttttgcc gatttggca gggactgag aaagttcta gtttatgac agcaacggg atgatgcaa acgattccc actgattta gggatacag actttacag aggtgtatt atgtcgctg tctgatttt aactgcagt tttgtgccg tacactcaa tccgttttg acgacacta ctgaaagac tcgttcggg gggcgcgca aagacactt ctgatctgt tgcgtgtcc ccccttgct caagacttc accgagact ttgtataca cttcagttg ggtttgagg gctcaaatg atcaaaaat ttcgtcact gttaactca tacgctacg tttgagtca acacaagag aattgtgat gttttcggc ctacagttc gccgccaat caactcctg aaacttgtc tcgaacgct gaagagttg ttccaacga ttggtagcg aacgacttg ttaccaaaa agcgaacaa gaacaaata tcacagtgg ttgatgctc aaacaagaa tgcgaagac tgtttgagt gaaaattca gagcctcat agatcactc gagcggatt gaggaggaa attgagagt gatagtagt gagagcgag gcgattgag gaggaggag aaggagagt ctccttgat agggttcaa ggtttgatg gagactttt cgctcaaac accgataaa ttagtctct gaggctaat tgtgcaaac gttacgaat tcttgtacc aaagaaagt atgaatagc tcgaataat gagtatcat atgaagggg gcacggggg cgcagaggg agtatccac tcggctgaa gaactaaac ccttgcttg tccctcaat acgttacgt attaatgac gatgtcgag agtcaaaaa atcgcagtt acgtcgtac gataacaag aagaaaatc cttaaacaa atcgccact gcgttagaa ttgaatcaa aaaaagatc tctgatttg gaaaaaaca attcgggtc aaggaaaac ctcatggaa aagctcctg aagcacaaa gacacaaaa tcgagtgct caccataaa atcgaacaa aaatgccag ttgttacgc aaggagtat aaaaacgcc gaggtcaag ttcttgcaa gcgaaatta caaaaaaat caccacttg gaagggaaa tacaagagc gaagttgtg gcccttgag gagcgactg cgagacacc gaatcgttg aaaaacctg accgaagac gggaacaaa ttgctagaa ttagaaagt agtctacat acagatcgg gagaagttg aaaaagtac aaacaagag aaagaaaag tataaacaa atctacgag aaacagctg aaggaggag cgctctaag tcgaaagcg agttgcgag agcgtgccg aaaaccccc gatgttaaa gtcgttgct ttaattaaa ccaaacgtt tcgctaaat agcgaagaa cttgaaagt ctgagacac gaaattcgc aacttgcgc aaaacccgc gactattta ttagaattg cggtgcaaa atcacgctc aaatcccag aataaaaaa ttcctgaac gactccgac gaacgcaag ttgctgcag tacgaggag gctattgag gcaatagac ttgatcata gaatacaaa aactcgatt ttgtgtggg cacctgcca gtcaaagag attgaggat catggcgat cggatgctg atggagcgg ttcctcaaa ctgagtgag aacgaaatg aggatcctc cttcataga tatttcgag aagattatc gatttgagg agcagcagc aaaaagctc gatttggag attttggat attgaaaac caacacgaa aatctcgca tatcgtgtg caaaaactc agtcataac ttacaactg gttaggtct gacgctgaa cggaggata gcctcgctc cagcaacaa cacgaagac aagttgtac ttggtcttg aggaacctg acgggagat ggggctgat aatgagagg ataagtcgg gtcttgggg tcgagacat tccgcagtg acggttcaa gtggcaggc gccagtaaa caagtggac aagaatagg ttcatagcg aagtggacg cgataccgc caggagacc gttccgaga cagctgcaa tcggtgccc ccacaggcc aaggtcacc attgagaaa aacaaaatc attctgcag aaaaccaac aagaactaa
- >TC007589 RA
agttgtgtt gtttgaggc agctgtaaa atgcattta aaatccatt gatttttaa taaattaac ccaaaccgc cgcaaccgc caggttata caatgggtt gaattctcc caggatgga gatgcacat agaaacggc ggtccgcat ccatacgtc gggctactc ccccaacga catggtttg cgtgcactc caaccccgt caacaacac catccaact cgcgaattc gcaaaacta ccccgtcaa ttacgccct tccagccga ctgttgcca aaacacggt gttcacaac cacggcagc ccctttggt gaattattt ggtggaagg gtgcgatgt ttcggtggt cacgctggg ccagtccgg gactggaaa gtcctacac gctcctagg cccaggttt ccactgtgc ggctagcga ggctgatca cgggattat cccgcggtt tattcgcga agttttcgc caaaatgaa acaatacag agacaggaa ttggtgcgt gcatatcgc ttggtcgca gatttgtgg cgaaaacgt ccaggattt gctaggcgt tgggagcat cgagtgccg gaatatcag tgacgtgtt tcagttgat tcaagtcgg aatgtcgaa tatagcccc caagtgcgc ccacacgtt gttcacact cactttgga gcagcagtg ggtggtgga gacaagtgt gcaacatcg cgtctcaac tgcgagttt tgccgattt ggcagggac tgagaaagt tctagttta tgacagcaa cgggatgat gcaaacgat tcccactga tttagggat acagacttt acagaggtg tattatgtc gctgtctga ttttaactg cagttttgt gccgtacac tcaatccgt tttgacgac actactgaa agactcgtt cggggggcg cgcaaagac acttctgat ctgttgcgt gtcccccct tgctcaaga cttcaccga gactttgta tacacttca gttgggttt gagggctca aatgatcaa aaatttcgt cactgttaa ctcatacgc tacgtttga gtcaacaca agagaattg tgatgtttt cggcctaca gttcgccgc caatcaact cctgaaact tgtctcgaa cgctgaaga gttgttcca acgattggt agcgaacga cttgttacc aaaaagcga acaagaaca aatatcaca gtggttgat gctcaaaca agaatgcga agactgttt gagtgaaaa ttcagagcc tcatagatc actcgagcg gattgagga ggaaattga gagtgatag tagtgagag cgaggcgat tgaggagga ggagaagga gagtctcct tgatagggt tcaaggttt gatggagac ttttcgctc aaacaccga taaattagt ctctgaggc taattgtgc aaacgttac gaattcttg taccaaaga aagtatgaa tagctcgaa taatgagta tcatatgaa gggggcacg ggggcgcag agggagtat ccactcggc tgaagaact aaacccttg cttgtccct caatacgtt acgtattaa tgacgatgt cgagagtca aaaaatcgc agttacgtc gtacgataa caagaagaa aatccttaa acaaatcgc cactgcgtt agaattgaa tcaaaaaaa gatctctga tttggaaaa aacaattcg ggtcaagga aaacctcat ggaaaagct cctgaagca caaagacac aaaatcgag tgctcacca taaaatcga acaaaaatg ccagttgtt acgcaagga gtataaaaa cgccgaggt caagttctt gcaagcgaa attacaaaa aaatcacca cttggaagg gaaatacaa gagcgaagt tgtggccct tgaggagcg actgcgaga caccgaatc gttgaaaaa cctgaccga agacgggaa caaattgct agaattaga aagtagtct acatacaga tcgggagaa gttgaaaaa gtacaaaca agagaaaga aaagtataa acaaatcta cgagaaaca gctgaagga ggagcgctc taagtcgaa agcgagttg cgagagcgt gccgaaaac ccccgatgt taaagtcgt tgctttaat taaaccaaa cgtttcgct aaatagcga agaacttga aagtctgag acacgaaat tcgcaactt gcgcaaaac ccgcgacta tttattaga attgcggtg caaaatcac gctcaaatc ccagaataa aaaattcct gaacgactc cgacgaacg caagttgct gcagtacga ggaggctat tgaggcaat agacttgat catagaata caaaaactc gattttgtg tgggcacct gccagtcaa agagattga ggatcatgg cgatcggat gctgatgga gcggttcct caaactgag tgagaacga aatgaggat cctccttca tagatattt cgagaagat tatcgattt gaggagcag cagcaaaaa gctcgattt ggagatttt ggatattga aaaccaaca cgaaaatct cgcatatcg tgtgcaaaa actcagtca taacttaca actggttag gtctgacgc tgaacggag gatagcctc gctccagca acaacacga agacaagtt gtacttggt cttgaggaa cctgacggg agatggggc tgataatga gaggataag tcgggtctt ggggtcgag acattccgc agtgacggt tcaagtggc aggcgccag taaacaagt ggacaagaa taggttcat agcgaagtg gacgcgata ccgccagga gaccgttcc gagacagct gcaatcggt gcccccaca ggccaaggt caccattga gaaaaacaa aatcattct gcagaaaac caacaagaa ctaattgta atttattgt aggctaggg taggacaat aatgatgta atcgtggtt tatcaaata aatgtgatg acttcag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
