Status
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Gene information
General information
TC007589 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2753907..2756902
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007589
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01226

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007589 RA
      MEMHIETAV RIHTSGYSP NDMVCVHSN PVNNTIQLA 
NSQNYPVNY ALPADCCQN TVFTTTAAP LVNYLVEGC 
DVSVVTLGQ SGTGKSYTL LGPGFHCAA SEADHGIIP 
RFIREVFAK MKQYRDRNW CVHIAWSQI CGENVQDLL 
GVGSIECRN ISDVFQLIQ VGMSNIAPK CAHTLFTLT 
LEQQWVVET SVQHRVSTA SFADLAGTE KVLVYDSNG 
MMQTIPTDL GIQTLQRCI MSLSDFNCS FVPYTQSVL 
TTLLKDSFG GRAKTLLIC CVSPLAQDF TETLYTLQL 
GLRAQMIKN FVTVNSYAT FESTQENCD VFGLQFAAN 
QLLKLVSNA EELFQRLVA NDLLPKSEQ EQISQWLML 
KQECEDCLS ENSEPHRSL ERIEEEIES DSSESEAIE 
EEEKESLLD RVQGLMETF RSNTDKLVS EANCANVTN 
SCTKESMNS SNNEYHMKG ARGRRGSIH SAEELNPCL 
SLNTLRIND DVESQKIAV TSYDNKKKI LKQIATALE 
LNQKKISDL EKTIRVKEN LMEKLLKHK DTKSSAHHK 
IEQKCQLLR KEYKNAEVK FLQAKLQKN HHLEGKYKS 
EVVALEERL RDTESLKNL TEDGNKLLE LESSLHTDR 
EKLKKYKQE KEKYKQIYE KQLKEERSK SKASCESVP 
KTPDVKVVA LIKPNVSLN SEELESLRH EIRNLRKTR 
DYLLELRCK ITLKSQNKK FLNDSDERK LLQYEEAIE 
AIDLIIEYK NSILCGHLP VKEIEDHGD RMLMERFLK 
LSENEMRIL LHRYFEKII DLRSSSKKL DLEILDIEN 
QHENLAYRV QKLSHNLQL VRSDAERRI ASLQQQHED 
KLYLVLRNL TGDGADNER ISRVLGSRH SAVTVQVAG 
ASKQVDKNR FIAKWTRYR QETVPRQLQ SVPPQAKVT 
IEKNKIILQ KTNKN
    CDS sequence
    • >TC007589 RA
      atggagatg cacatagaa acggcggtc cgcatccat 
acgtcgggc tactccccc aacgacatg gtttgcgtg 
cactccaac cccgtcaac aacaccatc caactcgcg 
aattcgcaa aactacccc gtcaattac gcccttcca 
gccgactgt tgccaaaac acggtgttc acaaccacg 
gcagcccct ttggtgaat tatttggtg gaagggtgc 
gatgtttcg gtggtcacg ctgggccag tccgggact 
ggaaagtcc tacacgctc ctaggccca ggtttccac 
tgtgcggct agcgaggct gatcacggg attatcccg 
cggtttatt cgcgaagtt ttcgccaaa atgaaacaa 
tacagagac aggaattgg tgcgtgcat atcgcttgg 
tcgcagatt tgtggcgaa aacgtccag gatttgcta 
ggcgttggg agcatcgag tgccggaat atcagtgac 
gtgtttcag ttgattcaa gtcggaatg tcgaatata 
gcccccaag tgcgcccac acgttgttc acactcact 
ttggagcag cagtgggtg gtggagaca agtgtgcaa 
catcgcgtc tcaactgcg agttttgcc gatttggca 
gggactgag aaagttcta gtttatgac agcaacggg 
atgatgcaa acgattccc actgattta gggatacag 
actttacag aggtgtatt atgtcgctg tctgatttt 
aactgcagt tttgtgccg tacactcaa tccgttttg 
acgacacta ctgaaagac tcgttcggg gggcgcgca 
aagacactt ctgatctgt tgcgtgtcc ccccttgct 
caagacttc accgagact ttgtataca cttcagttg 
ggtttgagg gctcaaatg atcaaaaat ttcgtcact 
gttaactca tacgctacg tttgagtca acacaagag 
aattgtgat gttttcggc ctacagttc gccgccaat 
caactcctg aaacttgtc tcgaacgct gaagagttg 
ttccaacga ttggtagcg aacgacttg ttaccaaaa 
agcgaacaa gaacaaata tcacagtgg ttgatgctc 
aaacaagaa tgcgaagac tgtttgagt gaaaattca 
gagcctcat agatcactc gagcggatt gaggaggaa 
attgagagt gatagtagt gagagcgag gcgattgag 
gaggaggag aaggagagt ctccttgat agggttcaa 
ggtttgatg gagactttt cgctcaaac accgataaa 
ttagtctct gaggctaat tgtgcaaac gttacgaat 
tcttgtacc aaagaaagt atgaatagc tcgaataat 
gagtatcat atgaagggg gcacggggg cgcagaggg 
agtatccac tcggctgaa gaactaaac ccttgcttg 
tccctcaat acgttacgt attaatgac gatgtcgag 
agtcaaaaa atcgcagtt acgtcgtac gataacaag 
aagaaaatc cttaaacaa atcgccact gcgttagaa 
ttgaatcaa aaaaagatc tctgatttg gaaaaaaca 
attcgggtc aaggaaaac ctcatggaa aagctcctg 
aagcacaaa gacacaaaa tcgagtgct caccataaa 
atcgaacaa aaatgccag ttgttacgc aaggagtat 
aaaaacgcc gaggtcaag ttcttgcaa gcgaaatta 
caaaaaaat caccacttg gaagggaaa tacaagagc 
gaagttgtg gcccttgag gagcgactg cgagacacc 
gaatcgttg aaaaacctg accgaagac gggaacaaa 
ttgctagaa ttagaaagt agtctacat acagatcgg 
gagaagttg aaaaagtac aaacaagag aaagaaaag 
tataaacaa atctacgag aaacagctg aaggaggag 
cgctctaag tcgaaagcg agttgcgag agcgtgccg 
aaaaccccc gatgttaaa gtcgttgct ttaattaaa 
ccaaacgtt tcgctaaat agcgaagaa cttgaaagt 
ctgagacac gaaattcgc aacttgcgc aaaacccgc 
gactattta ttagaattg cggtgcaaa atcacgctc 
aaatcccag aataaaaaa ttcctgaac gactccgac 
gaacgcaag ttgctgcag tacgaggag gctattgag 
gcaatagac ttgatcata gaatacaaa aactcgatt 
ttgtgtggg cacctgcca gtcaaagag attgaggat 
catggcgat cggatgctg atggagcgg ttcctcaaa 
ctgagtgag aacgaaatg aggatcctc cttcataga 
tatttcgag aagattatc gatttgagg agcagcagc 
aaaaagctc gatttggag attttggat attgaaaac 
caacacgaa aatctcgca tatcgtgtg caaaaactc 
agtcataac ttacaactg gttaggtct gacgctgaa 
cggaggata gcctcgctc cagcaacaa cacgaagac 
aagttgtac ttggtcttg aggaacctg acgggagat 
ggggctgat aatgagagg ataagtcgg gtcttgggg 
tcgagacat tccgcagtg acggttcaa gtggcaggc 
gccagtaaa caagtggac aagaatagg ttcatagcg 
aagtggacg cgataccgc caggagacc gttccgaga 
cagctgcaa tcggtgccc ccacaggcc aaggtcacc 
attgagaaa aacaaaatc attctgcag aaaaccaac 
aagaactaa
    mRNA sequence
    • >TC007589 RA
      agttgtgtt gtttgaggc agctgtaaa atgcattta 
aaatccatt gatttttaa taaattaac ccaaaccgc 
cgcaaccgc caggttata caatgggtt gaattctcc 
caggatgga gatgcacat agaaacggc ggtccgcat 
ccatacgtc gggctactc ccccaacga catggtttg 
cgtgcactc caaccccgt caacaacac catccaact 
cgcgaattc gcaaaacta ccccgtcaa ttacgccct 
tccagccga ctgttgcca aaacacggt gttcacaac 
cacggcagc ccctttggt gaattattt ggtggaagg 
gtgcgatgt ttcggtggt cacgctggg ccagtccgg 
gactggaaa gtcctacac gctcctagg cccaggttt 
ccactgtgc ggctagcga ggctgatca cgggattat 
cccgcggtt tattcgcga agttttcgc caaaatgaa 
acaatacag agacaggaa ttggtgcgt gcatatcgc 
ttggtcgca gatttgtgg cgaaaacgt ccaggattt 
gctaggcgt tgggagcat cgagtgccg gaatatcag 
tgacgtgtt tcagttgat tcaagtcgg aatgtcgaa 
tatagcccc caagtgcgc ccacacgtt gttcacact 
cactttgga gcagcagtg ggtggtgga gacaagtgt 
gcaacatcg cgtctcaac tgcgagttt tgccgattt 
ggcagggac tgagaaagt tctagttta tgacagcaa 
cgggatgat gcaaacgat tcccactga tttagggat 
acagacttt acagaggtg tattatgtc gctgtctga 
ttttaactg cagttttgt gccgtacac tcaatccgt 
tttgacgac actactgaa agactcgtt cggggggcg 
cgcaaagac acttctgat ctgttgcgt gtcccccct 
tgctcaaga cttcaccga gactttgta tacacttca 
gttgggttt gagggctca aatgatcaa aaatttcgt 
cactgttaa ctcatacgc tacgtttga gtcaacaca 
agagaattg tgatgtttt cggcctaca gttcgccgc 
caatcaact cctgaaact tgtctcgaa cgctgaaga 
gttgttcca acgattggt agcgaacga cttgttacc 
aaaaagcga acaagaaca aatatcaca gtggttgat 
gctcaaaca agaatgcga agactgttt gagtgaaaa 
ttcagagcc tcatagatc actcgagcg gattgagga 
ggaaattga gagtgatag tagtgagag cgaggcgat 
tgaggagga ggagaagga gagtctcct tgatagggt 
tcaaggttt gatggagac ttttcgctc aaacaccga 
taaattagt ctctgaggc taattgtgc aaacgttac 
gaattcttg taccaaaga aagtatgaa tagctcgaa 
taatgagta tcatatgaa gggggcacg ggggcgcag 
agggagtat ccactcggc tgaagaact aaacccttg 
cttgtccct caatacgtt acgtattaa tgacgatgt 
cgagagtca aaaaatcgc agttacgtc gtacgataa 
caagaagaa aatccttaa acaaatcgc cactgcgtt 
agaattgaa tcaaaaaaa gatctctga tttggaaaa 
aacaattcg ggtcaagga aaacctcat ggaaaagct 
cctgaagca caaagacac aaaatcgag tgctcacca 
taaaatcga acaaaaatg ccagttgtt acgcaagga 
gtataaaaa cgccgaggt caagttctt gcaagcgaa 
attacaaaa aaatcacca cttggaagg gaaatacaa 
gagcgaagt tgtggccct tgaggagcg actgcgaga 
caccgaatc gttgaaaaa cctgaccga agacgggaa 
caaattgct agaattaga aagtagtct acatacaga 
tcgggagaa gttgaaaaa gtacaaaca agagaaaga 
aaagtataa acaaatcta cgagaaaca gctgaagga 
ggagcgctc taagtcgaa agcgagttg cgagagcgt 
gccgaaaac ccccgatgt taaagtcgt tgctttaat 
taaaccaaa cgtttcgct aaatagcga agaacttga 
aagtctgag acacgaaat tcgcaactt gcgcaaaac 
ccgcgacta tttattaga attgcggtg caaaatcac 
gctcaaatc ccagaataa aaaattcct gaacgactc 
cgacgaacg caagttgct gcagtacga ggaggctat 
tgaggcaat agacttgat catagaata caaaaactc 
gattttgtg tgggcacct gccagtcaa agagattga 
ggatcatgg cgatcggat gctgatgga gcggttcct 
caaactgag tgagaacga aatgaggat cctccttca 
tagatattt cgagaagat tatcgattt gaggagcag 
cagcaaaaa gctcgattt ggagatttt ggatattga 
aaaccaaca cgaaaatct cgcatatcg tgtgcaaaa 
actcagtca taacttaca actggttag gtctgacgc 
tgaacggag gatagcctc gctccagca acaacacga 
agacaagtt gtacttggt cttgaggaa cctgacggg 
agatggggc tgataatga gaggataag tcgggtctt 
ggggtcgag acattccgc agtgacggt tcaagtggc 
aggcgccag taaacaagt ggacaagaa taggttcat 
agcgaagtg gacgcgata ccgccagga gaccgttcc 
gagacagct gcaatcggt gcccccaca ggccaaggt 
caccattga gaaaaacaa aatcattct gcagaaaac 
caacaagaa ctaattgta atttattgt aggctaggg 
taggacaat aatgatgta atcgtggtt tatcaaata 
aatgtgatg acttcag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY