Status
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Gene information
General information
TC007589 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2753907..2756902
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007589
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01226

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007589 RA
      MEMHIETAV RIHTSGYSP NDMVCVHSN PVNNTIQLA 
      NSQNYPVNY ALPADCCQN TVFTTTAAP LVNYLVEGC 
      DVSVVTLGQ SGTGKSYTL LGPGFHCAA SEADHGIIP 
      RFIREVFAK MKQYRDRNW CVHIAWSQI CGENVQDLL 
      GVGSIECRN ISDVFQLIQ VGMSNIAPK CAHTLFTLT 
      LEQQWVVET SVQHRVSTA SFADLAGTE KVLVYDSNG 
      MMQTIPTDL GIQTLQRCI MSLSDFNCS FVPYTQSVL 
      TTLLKDSFG GRAKTLLIC CVSPLAQDF TETLYTLQL 
      GLRAQMIKN FVTVNSYAT FESTQENCD VFGLQFAAN 
      QLLKLVSNA EELFQRLVA NDLLPKSEQ EQISQWLML 
      KQECEDCLS ENSEPHRSL ERIEEEIES DSSESEAIE 
      EEEKESLLD RVQGLMETF RSNTDKLVS EANCANVTN 
      SCTKESMNS SNNEYHMKG ARGRRGSIH SAEELNPCL 
      SLNTLRIND DVESQKIAV TSYDNKKKI LKQIATALE 
      LNQKKISDL EKTIRVKEN LMEKLLKHK DTKSSAHHK 
      IEQKCQLLR KEYKNAEVK FLQAKLQKN HHLEGKYKS 
      EVVALEERL RDTESLKNL TEDGNKLLE LESSLHTDR 
      EKLKKYKQE KEKYKQIYE KQLKEERSK SKASCESVP 
      KTPDVKVVA LIKPNVSLN SEELESLRH EIRNLRKTR 
      DYLLELRCK ITLKSQNKK FLNDSDERK LLQYEEAIE 
      AIDLIIEYK NSILCGHLP VKEIEDHGD RMLMERFLK 
      LSENEMRIL LHRYFEKII DLRSSSKKL DLEILDIEN 
      QHENLAYRV QKLSHNLQL VRSDAERRI ASLQQQHED 
      KLYLVLRNL TGDGADNER ISRVLGSRH SAVTVQVAG 
      ASKQVDKNR FIAKWTRYR QETVPRQLQ SVPPQAKVT 
      IEKNKIILQ KTNKN
    CDS sequence
    • >TC007589 RA
      atggagatg cacatagaa acggcggtc cgcatccat 
      acgtcgggc tactccccc aacgacatg gtttgcgtg 
      cactccaac cccgtcaac aacaccatc caactcgcg 
      aattcgcaa aactacccc gtcaattac gcccttcca 
      gccgactgt tgccaaaac acggtgttc acaaccacg 
      gcagcccct ttggtgaat tatttggtg gaagggtgc 
      gatgtttcg gtggtcacg ctgggccag tccgggact 
      ggaaagtcc tacacgctc ctaggccca ggtttccac 
      tgtgcggct agcgaggct gatcacggg attatcccg 
      cggtttatt cgcgaagtt ttcgccaaa atgaaacaa 
      tacagagac aggaattgg tgcgtgcat atcgcttgg 
      tcgcagatt tgtggcgaa aacgtccag gatttgcta 
      ggcgttggg agcatcgag tgccggaat atcagtgac 
      gtgtttcag ttgattcaa gtcggaatg tcgaatata 
      gcccccaag tgcgcccac acgttgttc acactcact 
      ttggagcag cagtgggtg gtggagaca agtgtgcaa 
      catcgcgtc tcaactgcg agttttgcc gatttggca 
      gggactgag aaagttcta gtttatgac agcaacggg 
      atgatgcaa acgattccc actgattta gggatacag 
      actttacag aggtgtatt atgtcgctg tctgatttt 
      aactgcagt tttgtgccg tacactcaa tccgttttg 
      acgacacta ctgaaagac tcgttcggg gggcgcgca 
      aagacactt ctgatctgt tgcgtgtcc ccccttgct 
      caagacttc accgagact ttgtataca cttcagttg 
      ggtttgagg gctcaaatg atcaaaaat ttcgtcact 
      gttaactca tacgctacg tttgagtca acacaagag 
      aattgtgat gttttcggc ctacagttc gccgccaat 
      caactcctg aaacttgtc tcgaacgct gaagagttg 
      ttccaacga ttggtagcg aacgacttg ttaccaaaa 
      agcgaacaa gaacaaata tcacagtgg ttgatgctc 
      aaacaagaa tgcgaagac tgtttgagt gaaaattca 
      gagcctcat agatcactc gagcggatt gaggaggaa 
      attgagagt gatagtagt gagagcgag gcgattgag 
      gaggaggag aaggagagt ctccttgat agggttcaa 
      ggtttgatg gagactttt cgctcaaac accgataaa 
      ttagtctct gaggctaat tgtgcaaac gttacgaat 
      tcttgtacc aaagaaagt atgaatagc tcgaataat 
      gagtatcat atgaagggg gcacggggg cgcagaggg 
      agtatccac tcggctgaa gaactaaac ccttgcttg 
      tccctcaat acgttacgt attaatgac gatgtcgag 
      agtcaaaaa atcgcagtt acgtcgtac gataacaag 
      aagaaaatc cttaaacaa atcgccact gcgttagaa 
      ttgaatcaa aaaaagatc tctgatttg gaaaaaaca 
      attcgggtc aaggaaaac ctcatggaa aagctcctg 
      aagcacaaa gacacaaaa tcgagtgct caccataaa 
      atcgaacaa aaatgccag ttgttacgc aaggagtat 
      aaaaacgcc gaggtcaag ttcttgcaa gcgaaatta 
      caaaaaaat caccacttg gaagggaaa tacaagagc 
      gaagttgtg gcccttgag gagcgactg cgagacacc 
      gaatcgttg aaaaacctg accgaagac gggaacaaa 
      ttgctagaa ttagaaagt agtctacat acagatcgg 
      gagaagttg aaaaagtac aaacaagag aaagaaaag 
      tataaacaa atctacgag aaacagctg aaggaggag 
      cgctctaag tcgaaagcg agttgcgag agcgtgccg 
      aaaaccccc gatgttaaa gtcgttgct ttaattaaa 
      ccaaacgtt tcgctaaat agcgaagaa cttgaaagt 
      ctgagacac gaaattcgc aacttgcgc aaaacccgc 
      gactattta ttagaattg cggtgcaaa atcacgctc 
      aaatcccag aataaaaaa ttcctgaac gactccgac 
      gaacgcaag ttgctgcag tacgaggag gctattgag 
      gcaatagac ttgatcata gaatacaaa aactcgatt 
      ttgtgtggg cacctgcca gtcaaagag attgaggat 
      catggcgat cggatgctg atggagcgg ttcctcaaa 
      ctgagtgag aacgaaatg aggatcctc cttcataga 
      tatttcgag aagattatc gatttgagg agcagcagc 
      aaaaagctc gatttggag attttggat attgaaaac 
      caacacgaa aatctcgca tatcgtgtg caaaaactc 
      agtcataac ttacaactg gttaggtct gacgctgaa 
      cggaggata gcctcgctc cagcaacaa cacgaagac 
      aagttgtac ttggtcttg aggaacctg acgggagat 
      ggggctgat aatgagagg ataagtcgg gtcttgggg 
      tcgagacat tccgcagtg acggttcaa gtggcaggc 
      gccagtaaa caagtggac aagaatagg ttcatagcg 
      aagtggacg cgataccgc caggagacc gttccgaga 
      cagctgcaa tcggtgccc ccacaggcc aaggtcacc 
      attgagaaa aacaaaatc attctgcag aaaaccaac 
      aagaactaa
    mRNA sequence
    • >TC007589 RA
      agttgtgtt gtttgaggc agctgtaaa atgcattta 
      aaatccatt gatttttaa taaattaac ccaaaccgc 
      cgcaaccgc caggttata caatgggtt gaattctcc 
      caggatgga gatgcacat agaaacggc ggtccgcat 
      ccatacgtc gggctactc ccccaacga catggtttg 
      cgtgcactc caaccccgt caacaacac catccaact 
      cgcgaattc gcaaaacta ccccgtcaa ttacgccct 
      tccagccga ctgttgcca aaacacggt gttcacaac 
      cacggcagc ccctttggt gaattattt ggtggaagg 
      gtgcgatgt ttcggtggt cacgctggg ccagtccgg 
      gactggaaa gtcctacac gctcctagg cccaggttt 
      ccactgtgc ggctagcga ggctgatca cgggattat 
      cccgcggtt tattcgcga agttttcgc caaaatgaa 
      acaatacag agacaggaa ttggtgcgt gcatatcgc 
      ttggtcgca gatttgtgg cgaaaacgt ccaggattt 
      gctaggcgt tgggagcat cgagtgccg gaatatcag 
      tgacgtgtt tcagttgat tcaagtcgg aatgtcgaa 
      tatagcccc caagtgcgc ccacacgtt gttcacact 
      cactttgga gcagcagtg ggtggtgga gacaagtgt 
      gcaacatcg cgtctcaac tgcgagttt tgccgattt 
      ggcagggac tgagaaagt tctagttta tgacagcaa 
      cgggatgat gcaaacgat tcccactga tttagggat 
      acagacttt acagaggtg tattatgtc gctgtctga 
      ttttaactg cagttttgt gccgtacac tcaatccgt 
      tttgacgac actactgaa agactcgtt cggggggcg 
      cgcaaagac acttctgat ctgttgcgt gtcccccct 
      tgctcaaga cttcaccga gactttgta tacacttca 
      gttgggttt gagggctca aatgatcaa aaatttcgt 
      cactgttaa ctcatacgc tacgtttga gtcaacaca 
      agagaattg tgatgtttt cggcctaca gttcgccgc 
      caatcaact cctgaaact tgtctcgaa cgctgaaga 
      gttgttcca acgattggt agcgaacga cttgttacc 
      aaaaagcga acaagaaca aatatcaca gtggttgat 
      gctcaaaca agaatgcga agactgttt gagtgaaaa 
      ttcagagcc tcatagatc actcgagcg gattgagga 
      ggaaattga gagtgatag tagtgagag cgaggcgat 
      tgaggagga ggagaagga gagtctcct tgatagggt 
      tcaaggttt gatggagac ttttcgctc aaacaccga 
      taaattagt ctctgaggc taattgtgc aaacgttac 
      gaattcttg taccaaaga aagtatgaa tagctcgaa 
      taatgagta tcatatgaa gggggcacg ggggcgcag 
      agggagtat ccactcggc tgaagaact aaacccttg 
      cttgtccct caatacgtt acgtattaa tgacgatgt 
      cgagagtca aaaaatcgc agttacgtc gtacgataa 
      caagaagaa aatccttaa acaaatcgc cactgcgtt 
      agaattgaa tcaaaaaaa gatctctga tttggaaaa 
      aacaattcg ggtcaagga aaacctcat ggaaaagct 
      cctgaagca caaagacac aaaatcgag tgctcacca 
      taaaatcga acaaaaatg ccagttgtt acgcaagga 
      gtataaaaa cgccgaggt caagttctt gcaagcgaa 
      attacaaaa aaatcacca cttggaagg gaaatacaa 
      gagcgaagt tgtggccct tgaggagcg actgcgaga 
      caccgaatc gttgaaaaa cctgaccga agacgggaa 
      caaattgct agaattaga aagtagtct acatacaga 
      tcgggagaa gttgaaaaa gtacaaaca agagaaaga 
      aaagtataa acaaatcta cgagaaaca gctgaagga 
      ggagcgctc taagtcgaa agcgagttg cgagagcgt 
      gccgaaaac ccccgatgt taaagtcgt tgctttaat 
      taaaccaaa cgtttcgct aaatagcga agaacttga 
      aagtctgag acacgaaat tcgcaactt gcgcaaaac 
      ccgcgacta tttattaga attgcggtg caaaatcac 
      gctcaaatc ccagaataa aaaattcct gaacgactc 
      cgacgaacg caagttgct gcagtacga ggaggctat 
      tgaggcaat agacttgat catagaata caaaaactc 
      gattttgtg tgggcacct gccagtcaa agagattga 
      ggatcatgg cgatcggat gctgatgga gcggttcct 
      caaactgag tgagaacga aatgaggat cctccttca 
      tagatattt cgagaagat tatcgattt gaggagcag 
      cagcaaaaa gctcgattt ggagatttt ggatattga 
      aaaccaaca cgaaaatct cgcatatcg tgtgcaaaa 
      actcagtca taacttaca actggttag gtctgacgc 
      tgaacggag gatagcctc gctccagca acaacacga 
      agacaagtt gtacttggt cttgaggaa cctgacggg 
      agatggggc tgataatga gaggataag tcgggtctt 
      ggggtcgag acattccgc agtgacggt tcaagtggc 
      aggcgccag taaacaagt ggacaagaa taggttcat 
      agcgaagtg gacgcgata ccgccagga gaccgttcc 
      gagacagct gcaatcggt gcccccaca ggccaaggt 
      caccattga gaaaaacaa aatcattct gcagaaaac 
      caacaagaa ctaattgta atttattgt aggctaggg 
      taggacaat aatgatgta atcgtggtt tatcaaata 
      aatgtgatg acttcag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY