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Gene information
General information
TC007586 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2760941..2761992
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007586
    iBeetle screen iB_01225

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    remarks: later than schedule, hours 96 pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007586 RA
      MLKTGAHRP CFALIRHFK ALRRLSTAA PEPSEQTRQ 
      KYEAARKSV TDLRRIYPE FLPDPKIEF RNPIREKLE 
      RSDMIARRA NIEIPEFYV GSILAVTLS DPHTPGKFN 
      RFVGICIKR EGCGLRSTF ILRNVIDHQ GIEILYELY 
      DPTIKSIEV LRLEKRLDG ELLYLRDAL PEYSTFDPN 
      MEVEILPEG APVPVNDIK VKLKPRPWV DRWERKHLK 
      GVQDLELPQ RFYDKAKEV DTPWEKYDL MKQYMRTIP 
      EEEQHEIFS ELQTHLHKS GVTQKVKRK RTFVKPTKL 
      A
    CDS sequence
    • >TC007586 RA
      atgttgaaa acgggtgca caccggcct tgttttgcc 
      ttaattcgg cactttaag gctttgcgc cgcctttcg 
      acagcagcc ccagaaccc agcgagcag acccgccag 
      aaatacgaa gccgcccgc aaaagcgta accgacctt 
      cgccgtatc taccccgag ttcctccca gaccccaaa 
      atcgaattc cgaaacccg atccgtgaa aaacttgaa 
      cgcagtgat atgattgct agacgtgca aatattgaa 
      attcctgaa ttttatgta gggtctatc cttgccgtg 
      acgttatcg gacccccac acccccggt aaattcaac 
      cgatttgtg gggatttgt atcaaaaga gaaggttgt 
      ggcttgaga tccactttt atccttcgg aatgtaata 
      gaccatcaa gggattgag attttgtac gagctgtac 
      gatcctaca attaagagt atcgaggtt ttgcggctt 
      gagaaacga cttgatggg gagttgttg tatttgagg 
      gacgcgttg cccgaatat agcactttt gatcccaat 
      atggaggtg gagattttg cctgaaggg gccccagtg 
      ccggtcaac gatattaaa gttaagttg aagccccgg 
      ccctgggtg gaccgctgg gagaggaag catctgaaa 
      ggggtgcag gatttggag ctgccacag cggttctat 
      gataaggca aaggaggtg gatacaccg tgggagaaa 
      tacgattta atgaaacaa tacatgagg actattcca 
      gaggaagag caacatgaa attttctca gaattgcaa 
      actcacttg cacaaatct ggtgttacc cagaaggtg 
      aagaggaag aggactttt gtcaaacca accaagtta 
      gcataa
    mRNA sequence
    • >TC007586 RA
      agccatact taacctcta ccatatcta ttttttttt 
      gtcaatgaa aatgttgaa aacgggtgc acaccggcc 
      ttgttttgc cttaattcg gcactttaa ggctttgcg 
      ccgcctttc gacagcagc cccagaacc cagcgagca 
      gacccgcca gaaatacga agccgcccg caaaagcgt 
      aaccgacct tcgccgtat ctaccccga gttcctccc 
      agaccccaa aatcgaatt ccgaaaccc gatccgtga 
      aaaacttga acgcagtga tatgattgc tagacgtgc 
      aaatattga aattcctga attttatgt agggtctat 
      ccttgccgt gacgttatc ggaccccca cacccccgg 
      taaattcaa ccgatttgt ggggatttg tatcaaaag 
      agaaggttg tggcttgag atccacttt tatccttcg 
      gaatgtaat agaccatca agggattga gattttgta 
      cgagctgta cgatcctac aattaagag tatcgaggt 
      tttgcggct tgagaaacg acttgatgg ggagttgtt 
      gtatttgag ggacgcgtt gcccgaata tagcacttt 
      tgatcccaa tatggaggt ggagatttt gcctgaagg 
      ggccccagt gccggtcaa cgatattaa agttaagtt 
      gaagccccg gccctgggt ggaccgctg ggagaggaa 
      gcatctgaa aggggtgca ggatttgga gctgccaca 
      gcggttcta tgataaggc aaaggaggt ggatacacc 
      gtgggagaa atacgattt aatgaaaca atacatgag 
      gactattcc agaggaaga gcaacatga aattttctc 
      agaattgca aactcactt gcacaaatc tggtgttac 
      ccagaaggt gaagaggaa gaggacttt tgtcaaacc 
      aaccaagtt agcataaat ttgtttatt aagtcaaga 
      aagccattt tatagca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY