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Gene information
General information
TC007578 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2820369..2822462
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007578
    iBeetle screen iB_01224

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007578 RA
      MWRFRNVVR LSTCFKFRN RCICAPKFP SFQQNEEKR 
      GFNHPKTDS LAFLGFTGL VIASCDNRT QSNKRLFRA 
      AQYGVVEEL KTLIKNKAN LNARHELGW TPLMVAAVN 
      NQYEAVKAL LEAGAEPNL GEEFISAKR TAKEKGLHP 
      IEVLMTREE EFSNSLNAK VSFRGFTAL HYAVLINNL 
      SIVKLLIDQ GADPTIENE AGHTPLAYA VEGDLKNYL 
      QEKTSKFEE IKKQNELEE RRRFPLEER LKTRIVGQE 
      GAITIVSAT IRRKENGWG DDEHPLVFL FLGSSGIGK 
      TELAKQLAA YIHKEKPQA FIRLDMSEY QEKHEVAKL 
      IGAPPGYIG HDEGGQLTS RLKQCPNAV VLFDEVDKA 
      HPDVLTVLL QLFDEGRLT DGHGKTIEC KNAIFIMTS 
      NLASDEIAQ HGLKLRQEI ENLRANRLS NTTDEEISD 
      NITISRKFK DEVVKPILK RHFKRDEFL GRINEIVYF 
      LPFSRTELL QLVSREMSV WAQRAKDKH KIDLNWDRS 
      VESALADGY DVHYGARSI KYEVERRVV NQLAAAHES 
      GFIGKGSVV QVTTVWHEK TLSPEIKLR VKSKGAKGF 
      VDVENNKLP VRNVNSVF
    CDS sequence
    • >TC007578 RA
      atgtggaga tttaggaac gttgtgaga ctctccact 
      tgtttcaaa tttcgaaac cgctgcatt tgcgcaccc 
      aaatttcct agtttccag caaaatgag gaaaaacgg 
      ggcttcaac caccctaag acagattct ttggcgttt 
      ttagggttc acaggactt gtcattgct tcctgtgat 
      aaccgaacg caaagtaac aagagatta tttcgggct 
      gcccagtat ggcgttgta gaagaatta aaaactctt 
      attaaaaat aaagctaat ttaaacgca cgccacgag 
      ctgggctgg accccttta atggtggcc gcagtcaac 
      aatcaatat gaagcggtt aaagcactt ctagaagcg 
      ggggcagag cccaatttg ggtgaagaa tttatcagt 
      gctaagcgg acggctaag gaaaagggg ctgcaccca 
      atcgaagtt ctcatgact cgggaggaa gaattttca 
      aatagttta aacgccaaa gtgtcattt aggggtttt 
      acagcattg cattacgcc gtcttgatt aataatttg 
      agtattgtt aagcttcta atagatcag ggggctgac 
      cctactatc gaaaacgaa gccgggcat acccccctt 
      gcatatgct gttgaaggt gatttgaaa aactacttg 
      caagaaaaa acctcaaag tttgaagaa atcaaaaag 
      caaaacgaa ttggaagag cgccgaagg tttccgctg 
      gaggaaaga ctcaaaaca cgcatagtt ggccaagaa 
      ggcgcaatc acaatagtt tctgcaacc attcgtagg 
      aaggagaac ggttggggc gatgacgag catcctttg 
      gtgttttta tttttgggc tcttccggg ataggcaag 
      actgagttg gccaaacag ttggctgcg tacattcac 
      aaggagaag ccccaagct ttcatcaga ttggacatg 
      tccgagtat caggaaaaa cacgaagtt gctaagctg 
      attggggca cctccgggt tatatcgga catgacgaa 
      gggggccaa ttgacctcg cgtctaaaa cagtgtcca 
      aacgctgtt gttttgttt gatgaagtg gacaaagcc 
      caccctgac gtccttaca gttcttctt caattgttt 
      gacgagggg cgacttaca gacggtcat ggtaaaaca 
      atcgagtgt aagaatgcg attttcatc atgacttcg 
      aatttggcc agcgacgaa atagcacag catggcttg 
      aagttacgg caggaaatt gaaaatttg cgggccaac 
      agattgagt aacacaaca gatgaagaa atttccgat 
      aatataacc atctcccgg aaattcaag gacgaggtc 
      gtcaagccc atcctgaag cggcacttt aaacgcgac 
      gagtttttg gggagaatc aacgaaatc gtctatttt 
      ttacccttt tccaggacc gaattattg cagcttgtt 
      agtcgggaa atgtcagtg tgggcacag cgagcgaaa 
      gataagcac aagatcgat ttaaattgg gatcgaagt 
      gtggagtcg gctcttgcc gacggttac gatgtccac 
      tacggtgct aggtcaata aaatatgaa gtggagaga 
      cgcgttgtt aatcagttg gctgcggct cacgaaagt 
      ggctttatt gggaaagga agtgtggtt caggttacc 
      acagtgtgg catgaaaaa accctaagt ccggagatt 
      aagttgagg gtgaagagt aaaggagcc aagggtttt 
      gttgatgtg gaaaataat aaattgcca gttagaaat 
      gttaattct gttttttaa
    mRNA sequence
    • >TC007578 RA
      tctgtaaat tcaatttta cgcgatgtg gagatttag 
      gaacgttgt gagactctc cacttgttt caaatttcg 
      aaaccgctg catttgcgc acccaaatt tcctagttt 
      ccagcaaaa tgaggaaaa acggggctt caaccaccc 
      taagacaga ttctttggc gtttttagg gttcacagg 
      acttgtcat tgcttcctg tgataaccg aacgcaaag 
      taacaagag attatttcg ggctgccca gtatggcgt 
      tgtagaaga attaaaaac tcttattaa aaataaagc 
      taatttaaa cgcacgcca cgagctggg ctggacccc 
      tttaatggt ggccgcagt caacaatca atatgaagc 
      ggttaaagc acttctaga agcgggggc agagcccaa 
      tttgggtga agaatttat cagtgctaa gcggacggc 
      taaggaaaa ggggctgca cccaatcga agttctcat 
      gactcggga ggaagaatt ttcaaatag tttaaacgc 
      caaagtgtc atttagggg ttttacagc attgcatta 
      cgccgtctt gattaataa tttgagtat tgttaagct 
      tctaataga tcagggggc tgaccctac tatcgaaaa 
      cgaagccgg gcatacccc ccttgcata tgctgttga 
      aggtgattt gaaaaacta cttgcaaga aaaaacctc 
      aaagtttga agaaatcaa aaagcaaaa cgaattgga 
      agagcgccg aaggtttcc gctggagga aagactcaa 
      aacacgcat agttggcca agaaggcgc aatcacaat 
      agtttctgc aaccattcg taggaagga gaacggttg 
      gggcgatga cgagcatcc tttggtgtt tttattttt 
      gggctcttc cgggatagg caagactga gttggccaa 
      acagttggc tgcgtacat tcacaagga gaagcccca 
      agctttcat cagattgga catgtccga gtatcagga 
      aaaacacga agttgctaa gctgattgg ggcacctcc 
      gggttatat cggacatga cgaaggggg ccaattgac 
      ctcgcgtct aaaacagtg tccaaacgc tgttgtttt 
      gtttgatga agtggacaa agcccaccc tgacgtcct 
      tacagttct tcttcaatt gtttgacga ggggcgact 
      tacagacgg tcatggtaa aacaatcga gtgtaagaa 
      tgcgatttt catcatgac ttcgaattt ggccagcga 
      cgaaatagc acagcatgg cttgaagtt acggcagga 
      aattgaaaa tttgcgggc caacagatt gagtaacac 
      aacagatga agaaatttc cgataatat aaccatctc 
      ccggaaatt caaggacga ggtcgtcaa gcccatcct 
      gaagcggca ctttaaacg cgacgagtt tttggggag 
      aatcaacga aatcgtcta ttttttacc cttttccag 
      gaccgaatt attgcagct tgttagtcg ggaaatgtc 
      agtgtgggc acagcgagc gaaagataa gcacaagat 
      cgatttaaa ttgggatcg aagtgtgga gtcggctct 
      tgccgacgg ttacgatgt ccactacgg tgctaggtc 
      aataaaata tgaagtgga gagacgcgt tgttaatca 
      gttggctgc ggctcacga aagtggctt tattgggaa 
      aggaagtgt ggttcaggt taccacagt gtggcatga 
      aaaaaccct aagtccgga gattaagtt gagggtgaa 
      gagtaaagg agccaaggg ttttgttga tgtggaaaa 
      taataaatt gccagttag aaatgttaa ttctgtttt 
      ttaacattt gtagagctg tatttatgt aaataaacg 
      ttgtttaga aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY