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Gene information
General information
TC007572 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 2912614..2914082
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007572
    iBeetle screen iB_01223
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007572 RA
      MNTWWFDQA AVNAFLTLQ EAHSTAVDA VVTGCSTCE 
      ELQCDHTVG YGGSPDENG ETTLDAMIF DGTTMNMGA 
      VGGLRRIKD AALAAKMVL QHTKHSFLV GDLATEFAK 
      NFELHEENL STNYSTNVW KEWKSRKCQ PNFWQNVNP 
      DPTKSCGPY QADSNEILA DEPWKIFNS DNHDTIGMI 
      AIDECGNVA AGTSTNGAS HKIPGRVGD SPIPGAGAY 
      ADSTVGAAV ATGDGDVMM RFLPSFLAV EEMRRGASP 
      AKAAQTAID RIAEKYPDF FGAVLVSTN KGEFGAACN 
      GMDSFPFCV ASEALGGVV VKTVNCTKK A
    CDS sequence
    • >TC007572 RA
      atgaatact tggtggttt gatcaagct gcggtaaat 
      gcattttta actttgcaa gaggctcat tcgaccgct 
      gtggacgct gtagtaacc ggatgttcc acttgcgaa 
      gaactgcag tgtgaccac actgttggg tatggggga 
      agtcccgac gaaaacggg gaaactacc cttgacgcc 
      atgatattt gacggcacc acaatgaat atgggggct 
      gttggtggt ttgagaaga atcaaagac gcggcactt 
      gccgcaaaa atggttcta cagcacaca aaacactca 
      tttcttgtg ggtgattta gcgactgaa tttgcaaaa 
      aatttcgaa ctgcatgaa gagaattta tctacgaat 
      tattccaca aacgtgtgg aaggagtgg aagtccaga 
      aagtgtcag cccaacttt tggcagaat gtaaatccg 
      gaccctacg aaatcatgc ggcccttac caagccgat 
      tcaaacgaa atcctggca gatgagcca tggaaaatt 
      tttaattcc gataaccat gacacaatt ggcatgatt 
      gccatagat gagtgtggg aacgttgca gcggggact 
      tcaacgaac ggagccagt cacaaaatc ccaggacgt 
      gtcggggat tcccccata cctggggcg ggcgcgtac 
      gccgatagc acagtcggg gcggctgtg gcaacaggc 
      gatggcgac gtaatgatg cgatttttg cccagtttt 
      ttggcagtg gaggagatg agacgcggc gcctcgcct 
      gcgaaagca gcccaaacg gccatcgat agaattgcg 
      gaaaaatac cccgatttt ttcggggct gttttagtg 
      agcactaac aagggggaa tttggggct gcctgtaac 
      ggaatggat tcgttcccg ttctgtgtg gcgagcgaa 
      gcgctcggt ggagtggtc gtgaagaca gttaattgc 
      accaaaaag gcatga
    mRNA sequence
    • >TC007572 RA
      acacgacta tgaaatcgt tgtttttaa gagtgaaca 
      gaattttac tgaataggg tttacttct atttttttg 
      cttaataaa atacaacac ttgacgctt atttaaaca 
      atattttta acacttatt ttgtagctt ctggattca 
      ctttataat gactctcaa attttttat tcttgttat 
      tacgatttt ccttatttt cttcgacaa tgctcacgc 
      ttctattcc tattgctat gaatacttg gtggtttga 
      tcaagctgc ggtaaatgc atttttaac tttgcaaga 
      ggctcattc gaccgctgt ggacgctgt agtaaccgg 
      atgttccac ttgcgaaga actgcagtg tgaccacac 
      tgttgggta tgggggaag tcccgacga aaacgggga 
      aactaccct tgacgccat gatatttga cggcaccac 
      aatgaatat gggggctgt tggtggttt gagaagaat 
      caaagacgc ggcacttgc cgcaaaaat ggttctaca 
      gcacacaaa acactcatt tcttgtggg tgatttagc 
      gactgaatt tgcaaaaaa tttcgaact gcatgaaga 
      gaatttatc tacgaatta ttccacaaa cgtgtggaa 
      ggagtggaa gtccagaaa gtgtcagcc caacttttg 
      gcagaatgt aaatccgga ccctacgaa atcatgcgg 
      cccttacca agccgattc aaacgaaat cctggcaga 
      tgagccatg gaaaatttt taattccga taaccatga 
      cacaattgg catgattgc catagatga gtgtgggaa 
      cgttgcagc ggggacttc aacgaacgg agccagtca 
      caaaatccc aggacgtgt cggggattc ccccatacc 
      tggggcggg cgcgtacgc cgatagcac agtcggggc 
      ggctgtggc aacaggcga tggcgacgt aatgatgcg 
      atttttgcc cagtttttt ggcagtgga ggagatgag 
      acgcggcgc ctcgcctgc gaaagcagc ccaaacggc 
      catcgatag aattgcgga aaaataccc cgatttttt 
      cggggctgt tttagtgag cactaacaa gggggaatt 
      tggggctgc ctgtaacgg aatggattc gttcccgtt 
      ctgtgtggc gagcgaagc gctcggtgg agtggtcgt 
      gaagacagt taattgcac caaaaaggc atgaaagct 
      gttattgta attacttta ttcaaaata aataaattt 
      ggatcga