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Gene information
General information
TC031099
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007571
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2914299..2917076
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031099
    iBeetle screen iB_01222

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 14.29% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    mesothorax shape irregular - Number of animals showing the phenotype: 9

    intercoxal process T3 shape irregular - Number of animals showing the phenotype: 9


    mesoepimeron shape irregular - Number of animals showing the phenotype: 9


    prothorax slightly dorsoventral-axis increased - Number of animals showing the phenotype: 9


    thorax thinner - Number of animals showing the phenotype: 9


    abdomen thinner - Number of animals showing the phenotype: 9

    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031099 RA
      MPIDLYYLP GSAPCRAVL LAAKAVGVE 
      LNLKLTDLM KGEHLTPEF IKINPQHTI 
      PTMVDNGFA LWESRAIMT YLADQYGKN 
      DALYPKDPK KRALVDQRL YFDIGTLYA 
      RFADYYYPV IFGGAEYEP AKLEKIKDA 
      FKFLEIFLE GQDFVAGNQ LTLADLSLL 
      ATVTTFEAV NFDLSPYKN VVNWLARAK 
      AAAPGYEEA NGKGAVIFK QMVENLTKK
    CDS sequence
    • >TC031099 RA
      atgcccatc gacctgtac tatcttcct ggaagtgcc 
      ccatgcagg gccgtcctt ttggcggcg aaagccgtc 
      ggtgtggag ctcaattta aaactcaca gacctcatg 
      aaaggcgaa cacctaacg cctgaattt attaagatc 
      aacccgcaa catacgatc ccgaccatg gtcgacaac 
      ggctttgcc ctgtgggag agcagggcc ataatgacc 
      tacctggcc gaccaatac ggcaaaaac gatgcgctt 
      tacccgaaa gacccgaaa aaacgcgcc ctggttgac 
      cagagattg tacttcgac attggcact ttgtacgcc 
      agatttgcc gattattac tacccggtg atttttgga 
      ggcgctgaa tacgagccc gccaaactg gaaaaaatc 
      aaagacgca ttcaagttc ctggagatt ttccttgag 
      ggtcaggac tttgttgcg ggaaatcag ctcactttg 
      gctgatttg tcgcttctt gcaactgtt acgacattc 
      gaagcagtg aattttgac ctttcgccg tacaaaaat 
      gtggttaat tggttggcg agagccaaa gctgcggcg 
      cctgggtat gaggaggcg aatgggaag ggggctgtg 
      atctttaag cagatggtc gaaaattta acgaaaaaa 
      taa
    mRNA sequence
    • >TC031099 RA
      tggaatgaa tagatgccg gcagtgcca acgccaaca 
      accagttca cgattgttc gtttgtaga ggcagttgt 
      tcggtggtc cgttgccgc ataaattct ttccaagtg 
      tctgaaact tttgattaa ttgatcaaa atgcccatc 
      gacctgtac tatcttcct ggaagtgcc ccatgcagg 
      gccgtcctt ttggcggcg aaagccgtc ggtgtggag 
      ctcaattta aaactcaca gacctcatg aaaggcgaa 
      cacctaacg cctgaattt attaagatc aacccgcaa 
      catacgatc ccgaccatg gtcgacaac ggctttgcc 
      ctgtgggag agcagggcc ataatgacc tacctggcc 
      gaccaatac ggcaaaaac gatgcgctt tacccgaaa 
      gacccgaaa aaacgcgcc ctggttgac cagagattg 
      tacttcgac attggcact ttgtacgcc agatttgcc 
      gattattac tacccggtg atttttgga ggcgctgaa 
      tacgagccc gccaaactg gaaaaaatc aaagacgca 
      ttcaagttc ctggagatt ttccttgag ggtcaggac 
      tttgttgcg ggaaatcag ctcactttg gctgatttg 
      tcgcttctt gcaactgtt acgacattc gaagcagtg 
      aattttgac ctttcgccg tacaaaaat gtggttaat 
      tggttggcg agagccaaa gctgcggcg cctgggtat 
      gaggaggcg aatgggaag ggggctgtg atctttaag 
      cagatggtc gaaaattta acgaaaaaa taaacacac 
      aattgtatc tcactgtaa tgtaataaa taaagtgca 
      ttattacag aatctgagt agtttactt tataagtaa 
      atagttgga gcagtcatt gcattcaac tagacactc 
      ttcttcgtc aagatattc cttgtgtat cgtaaaagt 
      gcccccaca acgacacga gtagtttgc gatattatc 
      aaaattaaa actaatttc ggttttatt taatcttat 
      gtggactaa accgttctc attatcgat ctctgaatc 
      taaatacct gtcatactc agctcttga attctcatt 
      ttttaaagt tttccacgc cttatcaat attgaggaa 
      attttcttg ggaaccgcc acttttatc aggatgata 
      ttgtcttat cagctgttg atagctttg tttcaattc 
      ttcgtatgt tgctgaccg gctgcagtt cagtagcga 
      gtaataatc ttcttttct tgcttgtgt agtcattat 
      ctgaaattc acctttaag aaaaaaaat ttgaatgtt 
      tttcctcac cagcaaggt ataattaaa ggtaaatat 
      aaattaaaa caattattg attttaagc ttgccccaa 
      gagatgtct tgtgctctg tcttgtgtg ttatagaca 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY