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Gene information
General information
TC007565 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2946289..2951750
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007565
    iBeetle screen iB_01221

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not completely molted - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    wings blistered - Number of animals showing the phenotype: 7

    dorsal thoracic muscles not present - Number of animals showing the phenotype: 2

    abdomen circumference increased -

    abdomen gin-traps orientation irregular - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007565 RA
      MEDNSLNHQ NGAQIGGPQ TVSVTNNKQ NEEANPKAQ 
      YSIPGILHF IQHEWARFE LERSQWEVD RAELQARIA 
      FLQGERKGQ ENLKNDLVR RIKMLEYAL KQERGKFHK 
      LKYGTELQQ GDMKPPIID EACESQMDI DQSYVAVSN 
      STWKQGRQL LRQYLQEIG YTDRIIDVR STRVRALLG 
      LNNNTEPEE NHNGSSVNG NESIKRASE TQGRRTPAK 
      KPQPSSLAE AMMLDTEAA VMANLDFLS NADVDMEDD 
      DDMSDDVDI EPTDDLDDD VKVVKRKGK LDSLGDDVD 
      AEAMEVLNE LNLLSESGE RQEFKNEHS EWKKGFQGG 
      VPKRPLGLN DDDNIDSAL GLGELAQLT VNNDAETTY 
      DIASSKEAF RKTWNAKYT LRSHFDGVR ALAFHPNES 
      VLITASEDH TLKLWNLQK TVPAKKSAS LDVEPLYTF 
      RNHTGPVLC LAISGTGEF CYSGGLDGV INCWNVPNS 
      NIDPYDLFE PDVLNTTLQ GHTDAVWGL SVLNSRQHL 
      LSCAADGTV KLWAPHSKV QLLNTFTSE QDGIPSSVD 
      FVRDQPNRI VAAYASSHC VVYDSETAK SVMRLESSQ 
      DSVNPNNKQ INRIVCHPT LPLTITAHE DRHVRFWDN 
      HTGKMVHSM VAHLEAVTS LAVDPNGLY LLSGSHDCS 
      IRLWNLDNK TCVQEITAH RKKFHESIL DVAFHPTRP 
      YIASAGADG LAKVFV
    CDS sequence
    • >TC007565 RA
      atggaagac aattcgcta aatcaccag aacggggcc 
      caaatcggg gggccccag accgtctcg gtgaccaac 
      aacaagcag aacgaagag gccaatccc aaagcacag 
      tattcaatt cctggaata ttgcatttt attcaacat 
      gaatgggca cggttcgaa ttggaaaga tcacagtgg 
      gaagtcgac agggctgaa ttacaagcc aggatcgcc 
      ttcctgcag ggcgaacgc aaaggccaa gaaaacttg 
      aaaaatgac ttggttcgc cgcataaaa atgctggag 
      tacgcgctg aagcaagaa cgaggcaaa tttcacaaa 
      ttgaaatac ggtactgaa ttgcaacaa ggagatatg 
      aagcctccg atcatagat gaagcgtgt gaatctcaa 
      atggatatt gaccaatcg tatgttgcc gtttctaat 
      tcaacatgg aaacaaggt cgtcagtta cttagacag 
      tatttacaa gaaatcggt tacaccgat cgaattatc 
      gatgtgaga tcaacacgc gttagagct cttctagga 
      ttgaataac aacaccgaa ccggaagaa aaccacaac 
      ggaagttcg gttaacggc aatgagtcg attaagcgc 
      gccagcgag acgcagggg agaagaact ccagctaag 
      aaacctcaa cccagctca ttggccgaa gcaatgatg 
      ctcgataca gaagcggcg gttatggcc aatttggac 
      ttcctgtct aatgctgat gttgacatg gaagacgat 
      gatgatatg agtgatgat gttgacatt gagcccact 
      gatgatctc gacgacgat gtgaaagtg gtgaaacga 
      aaaggtaaa ctggatagt ttgggagat gatgtggac 
      gccgaagcc atggaagtt ctaaacgaa ctcaatctt 
      ttgtccgag tctggagag cgacaggaa tttaagaat 
      gagcacagc gagtggaag aaaggtttt caaggtgga 
      gtgcctaag aggccgctg ggcctaaac gacgacgat 
      aacattgat tcggcgtta gggctcggc gaactggcc 
      caactgacg gtaaacaat gatgcagag actacgtac 
      gacattgcc agcagtaaa gaggcattt cggaagaca 
      tggaatgcc aaatacacg cttcgaagt cactttgac 
      ggggtccga gcgctagct tttcatcca aatgagtcc 
      gtactgatc acagctagc gaagaccac actttgaaa 
      ctgtggaat ttgcagaaa actgtaccc gcgaaaaaa 
      tcggcatcc cttgatgtg gaacccttg tacaccttt 
      aggaatcat actggaccc gttctttgt ttggcgatt 
      tccgggacc ggagaattt tgttattcc ggtggctta 
      gatggcgtg ataaactgt tggaatgtt cctaattct 
      aacatagat ccgtacgat ttgtttgaa cctgacgtt 
      ctaaacact acgttgcaa ggtcatact gacgctgtt 
      tgggggctg tctgtttta aattctcgg cagcatttg 
      ctctcatgt gcggccgat ggtactgtt aagttgtgg 
      gcaccgcat agcaaggta caattactg aacacgttc 
      acgtccgaa caggacggg atcccctca agtgtagat 
      ttcgtgaga gaccaacca aatcgtata gtcgcagct 
      tacgctagt tctcattgt gtagtatat gattctgaa 
      actgccaaa tccgtcatg cggctcgaa tccagtcaa 
      gacagtgtt aatcctaat aataagcaa ataaataga 
      atagtttgc catccgact ttacccttg accatcaca 
      gcacacgaa gatagacat gttcgtttc tgggataat 
      cacaccggc aaaatggtc cattctatg gttgctcat 
      ctcgaagct gtcactagc ctagcagta gatcctaac 
      gggttgtat ttattatca ggctcacat gattgttca 
      ataagatta tggaacttg gacaataag acttgcgtt 
      caagagatc actgcacac agaaagaag ttccacgaa 
      agcattcta gacgtcgct ttccacccg actcggccg 
      tatattgca agtgcgggg gctgatgga ctggccaaa 
      gtgtttgtc tga
    mRNA sequence
    • >TC007565 RA
      agagaatgt aatggcttc aggacctgt caactgtgt 
      tggacaaag aaagcgaat agcgcacgt agaaaacga 
      ttgaagtgg aaggaggaa aaaggaaat ctaccagat 
      gtgaaatgg ttgaatgaa tgtgaaatt tcgtttgga 
      aatcaatgt ttttatggt acacggccc taacaccaa 
      ttaaaccct gtcccaccg tcgaaatcc gtaaattgt 
      tgtgtttcg gaaaatgat ttcgtgaac agtgaagtg 
      acattcgaa tcccgagcg ttcaagtta aaatggcga 
      tttaatgaa taatggtgg ctttatgaa attttctag 
      tttcggcat cgacattat ggaagacaa ttcgctaaa 
      tcaccagaa cggggccca aatcggggg gccccagac 
      cgtctcggt gaccaacaa caagcagaa cgaagaggc 
      caatcccaa agcacagta ttcaattcc tggaatatt 
      gcattttat tcaacatga atgggcacg gttcgaatt 
      ggaaagatc acagtggga agtcgacag ggctgaatt 
      acaagccag gatcgcctt cctgcaggg cgaacgcaa 
      aggccaaga aaacttgaa aaatgactt ggttcgccg 
      cataaaaat gctggagta cgcgctgaa gcaagaacg 
      aggcaaatt tcacaaatt gaaatacgg tactgaatt 
      gcaacaagg agatatgaa gcctccgat catagatga 
      agcgtgtga atctcaaat ggatattga ccaatcgta 
      tgttgccgt ttctaattc aacatggaa acaaggtcg 
      tcagttact tagacagta tttacaaga aatcggtta 
      caccgatcg aattatcga tgtgagatc aacacgcgt 
      tagagctct tctaggatt gaataacaa caccgaacc 
      ggaagaaaa ccacaacgg aagttcggt taacggcaa 
      tgagtcgat taagcgcgc cagcgagac gcaggggag 
      aagaactcc agctaagaa acctcaacc cagctcatt 
      ggccgaagc aatgatgct cgatacaga agcggcggt 
      tatggccaa tttggactt cctgtctaa tgctgatgt 
      tgacatgga agacgatga tgatatgag tgatgatgt 
      tgacattga gcccactga tgatctcga cgacgatgt 
      gaaagtggt gaaacgaaa aggtaaact ggatagttt 
      gggagatga tgtggacgc cgaagccat ggaagttct 
      aaacgaact caatctttt gtccgagtc tggagagcg 
      acaggaatt taagaatga gcacagcga gtggaagaa 
      aggttttca aggtggagt gcctaagag gccgctggg 
      cctaaacga cgacgataa cattgattc ggcgttagg 
      gctcggcga actggccca actgacggt aaacaatga 
      tgcagagac tacgtacga cattgccag cagtaaaga 
      ggcatttcg gaagacatg gaatgccaa atacacgct 
      tcgaagtca ctttgacgg ggtccgagc gctagcttt 
      tcatccaaa tgagtccgt actgatcac agctagcga 
      agaccacac tttgaaact gtggaattt gcagaaaac 
      tgtacccgc gaaaaaatc ggcatccct tgatgtgga 
      acccttgta cacctttag gaatcatac tggacccgt 
      tctttgttt ggcgatttc cgggaccgg agaattttg 
      ttattccgg tggcttaga tggcgtgat aaactgttg 
      gaatgttcc taattctaa catagatcc gtacgattt 
      gtttgaacc tgacgttct aaacactac gttgcaagg 
      tcatactga cgctgtttg ggggctgtc tgttttaaa 
      ttctcggca gcatttgct ctcatgtgc ggccgatgg 
      tactgttaa gttgtgggc accgcatag caaggtaca 
      attactgaa cacgttcac gtccgaaca ggacgggat 
      cccctcaag tgtagattt cgtgagaga ccaaccaaa 
      tcgtatagt cgcagctta cgctagttc tcattgtgt 
      agtatatga ttctgaaac tgccaaatc cgtcatgcg 
      gctcgaatc cagtcaaga cagtgttaa tcctaataa 
      taagcaaat aaatagaat agtttgcca tccgacttt 
      acccttgac catcacagc acacgaaga tagacatgt 
      tcgtttctg ggataatca caccggcaa aatggtcca 
      ttctatggt tgctcatct cgaagctgt cactagcct 
      agcagtaga tcctaacgg gttgtattt attatcagg 
      ctcacatga ttgttcaat aagattatg gaacttgga 
      caataagac ttgcgttca agagatcac tgcacacag 
      aaagaagtt ccacgaaag cattctaga cgtcgcttt 
      ccacccgac tcggccgta tattgcaag tgcgggggc 
      tgatggact ggccaaagt gtttgtctg aattataag 
      taaacagga catcactta tattaaata gattttgtc 
      aatcgatac attcactgg ctttactat ttgtgtggg 
      ttaggtgta cataaaata ttctgaagg agtttgtat 
      atactacat gattgtact caaaggctt ttcttactt 
      taatttctc atgttttat ttatcacca aagtttaga 
      caaacaact tatgtacat gtttcctac actttgagc 
      ttatatgtg ctcacagaa tgatcttga tgtttcgta 
      ggagttaat ttttgttgt aggatagtc agcgttctg 
      ataaatccc atcatttgc aattatttt cttgtttct 
      ggacttgca taccaaaag aatcgcttt tgtgtacag 
      ctggccata cccaaagtg taagaaaaa attctcatt 
      ttacacaca atgtcgacg cgttttctt attttgtga 
      taactagtt tcaactcac taataaatg aattgccac 
      aggaaaaac atttttaaa agtgtatta atagtaacg 
      tttttataa tagtattaa aatagctgt tattttatt 
      attttaagc atataattt atcacgtat atttttaag 
      tttaatata gtccagtgc ataatagct aaagaaacg 
      taaagtagc agttaacaa tagtaagtg ataataagt 
      ataattgtg aatgtataa aagtgtatt taaatcata 
      ggataatat ttaagaaac tggtctctc tattagtta 
      tctgttttc taaagtcaa taatgtata tattagctt 
      aatcatttt cggttttga tagcgagat tgttgtgtt 
      tatgtaact ttttatagt ggctactat ttattttgt 
      acacaagtt ttaaactgt ttaaaatgc ttcgaaaca 
      gaacgataa gtcccgtat gatttcaac attgtagat 
      acaaagtca taggaacta cttacaccg tcatgttct 
      attttataa agaaatttc atgtttgca atgtataaa 
      acacctgta actttttta aaataacca taagtggtt 
      acgaagcgg tcaaatcac aaggaatga agaatgtat 
      gacttaatg tataaaaca taaaacaat aaactgtaa 
      atactcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY