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Gene information
General information
TC007562 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2983954..2986172
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007562
    iBeetle screen iB_01220

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants without visible segmentation but polarity - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle dorsaldecreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva appendages sometimesshape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Head appendages sometimes un-identifiable.
    head mostly decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 85.71% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 8

    antenna distal broader - Number of animals showing the phenotype: 8
    maxillary palp size increased - Number of animals showing the phenotype: 8

    wings shape irregular - Number of animals showing the phenotype: 8

    legs orientation irregular - Number of animals showing the phenotype: 8

    Adult morphology
    flagellomeres number decreased - Number of animals showing the phenotype: 5


    flagellomeres partially fused - Number of animals showing the phenotype: 5

    prothoracic leg shortened - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007562 RA
      MPNEARYAL LYSDYETST VLRLPFEKF SIITVGLPL 
      FAFLFCIIY SVIFHFETA TYTHCQVFN VLPSISAAI 
      GNFSPQRQV WQTAIVLHA VPRFSIAFV YLQHHQNVL 
      YQNDQWMGA VACVLNVVE NISLIILSF WTSSQHYPV 
      HEKAFITFI LMSEFYMIL IYILQRRCK KRHKESLKW 
      KKRLLITNI SSILVATYC FLRHNSYCE PYVYSGFAL 
      AEYTVVLTN MAFHMTAYL DFKNKDLVV YRYGVALTE 
      R
    CDS sequence
    • >TC007562 RA
      atgcctaat gaagctcgg tatgcgtta ctatacagt 
      gattacgaa acttccaca gttttgcgt ttgcctttt 
      gaaaaattt tcgattata accgtgggt ttgccgttg 
      tttgctttc ctgttttgc attatttat tcggtgatt 
      tttcatttc gaaaccgcc acttacacc cattgtcaa 
      gtgtttaat gttttaccg tccatttct gcggctatc 
      ggaaatttt tcaccacaa cgacaagtc tggcaaacg 
      gctatagtt ttacatgct gtgccaaga ttctcgatt 
      gcctttgtt tacctccaa caccaccaa aacgtttta 
      taccaaaat gaccaatgg atgggggcc gtcgcatgt 
      gttcttaat gttgttgaa aacatttcg ttgattata 
      ctgtctttt tggacctca tctcagcac tatcctgtt 
      catgaaaaa gccttcatc acctttata ttgatgtcg 
      gagttttat atgatttta atttacatt ctccaaaga 
      cgttgtaaa aagcgccac aaagaaagt ctgaaatgg 
      aagaaaaga ctactcatc acaaacata tcatctatt 
      ttagtagca acgtattgc tttttaagg cacaactca 
      tattgtgag ccttatgta tattctggt tttgccttg 
      gcagaatat acagtagta ttaacaaat atggcgttc 
      cacatgact gcttattta gattttaaa aataaagat 
      ttggtggtc tataggtac ggtgttgct ttaacagaa 
      agataa
    mRNA sequence
    • >TC007562 RA
      aatcaaaca gctgatcta acctaaaat gaattaagt 
      taagtgagt ttttaaatc taatctagc gaagtgtgt 
      ttcgaataa tgcctaatg aagctcggt atgcgttac 
      tatacagtg attacgaaa cttccacag ttttgcgtt 
      tgccttttg aaaaatttt cgattataa ccgtgggtt 
      tgccgttgt ttgctttcc tgttttgca ttatttatt 
      cggtgattt ttcatttcg aaaccgcca cttacaccc 
      attgtcaag tgtttaatg ttttaccgt ccatttctg 
      cggctatcg gaaattttt caccacaac gacaagtct 
      ggcaaacgg ctatagttt tacatgctg tgccaagat 
      tctcgattg cctttgttt acctccaac accaccaaa 
      acgttttat accaaaatg accaatgga tgggggccg 
      tcgcatgtg ttcttaatg ttgttgaaa acatttcgt 
      tgattatac tgtcttttt ggacctcat ctcagcact 
      atcctgttc atgaaaaag ccttcatca cctttatat 
      tgatgtcgg agttttata tgattttaa tttacattc 
      tccaaagac gttgtaaaa agcgccaca aagaaagtc 
      tgaaatgga agaaaagac tactcatca caaacatat 
      catctattt tagtagcaa cgtattgct ttttaaggc 
      acaactcat attgtgagc cttatgtat attctggtt 
      ttgccttgg cagaatata cagtagtat taacaaata 
      tggcgttcc acatgactg cttatttag attttaaaa 
      ataaagatt tggtggtct ataggtacg gtgttgctt 
      taacagaaa gataatatt tagttgtat agctagtta 
      tacaataaa tttttcgtt gtgtaggaa agtatcaaa 
      tggacatac agattgtat atttgtaat tattgccca 
      cacaccaaa acgatatgt attcgaaaa ttttacttt 
      ctggatctt caaaacgta taactttcc caaaaatgg 
      cacttacag attttagat tgtaaattg taagttcgt 
      gatctacga gttgattaa atgaaatgt cttctacgt 
      tttgaacgt ttttaactt gtaataaga taatatatt 
      ctgcatgta cactgccta aaaatcaag tgcacacag 
      tttatttca acagccaag tggtggctg gctgtgcac 
      ttgatttag attacaagc tgaagatat ttttgacga 
      aatgtaagg tcgtaatat cttcgaatt gttccagta 
      atgtagcta ttcctgcat ttcaagtaa tgagtgtgg 
      tttaaatgt tactctttt gtccattgt atctacttt 
      ttgatgtta caatgaaga gcacatttt atacttcgg 
      gcacagtag agatttact acttaattc acaagggtt 
      gcagtttaa tgttgtcgc gaaaaaacg accaaattt 
      tttcgtttc gaacttttt caaattttt agtgacaaa 
      aagacgtct aaaaataga atgttttag gcacttggt 
      atgaaagta cttatctgt gtgaatgta atcttgtaa 
      tataaacgt gctcagttg tgatatgta tttttgaga 
      agcgaaaga tttattata aatagacga aattggtat 
      tgaaaacat atcttgtat ttttcaaat aattattat 
      gattgaaaa aaaaatcgg gacggaaaa cacgaaacg 
      gtgttaata tttttagta attatgcga tggacgcat 
      ttagtgtca aattttgaa gaacaaaaa aattgatgt 
      cttcaccac ccaactttg ttttaagac gatgtgata 
      tgagtttgt gcttccgaa ggagcaatt tttttaatg 
      tcggtacat gtttatatc agtatatga ataattgtg 
      ataaaaaat tggttattt atgcatttc atttaagtt 
      ttacggatg gtaatggtt gtaaacaat gatataact 
      taaggaaat gtctcatta ttgtgcagt atttttgtc 
      ttaaaaaac tgttttttt ttcaaatgt ttattattt 
      cacatttaa gtgatgtgt tactgttaa tacgttgtt 
      aaactcgac tctttctaa ttgtactat tcttgtacg 
      tagctacct tttattaaa aaagttatt cttca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY